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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (6): 681-689.doi: 10.3736/jcim20120613

• Original Experimental Research • Previous Articles     Next Articles

Green synthesis, characterization and anticancer potential of platinum nanoparticles Bioplatin

 Yogesh Bendale(), Vineeta Bendale, Saili Paul, Soumya Sundar Bhattacharyya()   

  1. Research and Development Section, Cell and Molecular Biology Department, Rasayani Biologics Private Limited, Pune 411030, IndiaResearch and Development Section, Cell and Molecular Biology Department, Rasayani Biologics Private Limited, Pune 411030, India
  • Received:2012-01-05 Accepted:2012-02-12 Online:2012-06-20 Published:2018-06-15
  • Contact: Bendale Yogesh,Sundar Bhattacharyya Soumya E-mail:dr.bendale@gmail.com;ssbku13@gmail.com

Objective: In the present study, the anticancer potential of platinum nanoparticles Bioplatin is explored and the mode of interactions of Bioplatin with calf thymus DNA and honey was analyzed.
Methods: Bioplatin was synthesized with the help of green nanotechnology and characterized by particle size, zeta potential and surface morphology. The interaction of Bioplatin with DNA and honey was also checked with the help of circular dichroism spectroscopy and Fourier-transform infrared spectroscopy, respectively. The anticancer potential of Bioplatin was evaluated on peripheral blood mononuclear cells and A375 cells in vitro by analyzing results of MTT (3-(4,5)-dimethylthiahiazo-(-z-y1)-3,5-di-phenytetrazoliumromide), fluorescence microscopic studies and DNA fragmentation assay.
Results: Bioplatin exhibited a small particle size of 137.5 nm and a surface charge of –35.8 mV. Bioplatin interacted with DNA and brought in effective changes in structure and conformation of DNA, and formed a new complex that increased its stability of DNA intercalated with the base pair of DNA. In vitro studies demonstrated that Bioplatin arrested cell proliferation, and induced chromatin condensation and internucleosomal DNA fragmentation.
Conclusion: Bioplatin induces apoptosis in cancer cells and may have some beneficial effect against human carcinoma. It interacts with DNA, brings stabilization to DNA, and thus prevents the replication of DNA.

Key words: metal nanoparticles, antineoplastic agents, apoptosis, biosynthetic pathways

Figure 1

Average particle size obtained from dynamic light scattering data for Bioplatin"

Figure 2

Zeta potential of Bioplatin"

Figure 3

Surface morphology of Bioplatin observed by scanning electron microscopy (30.0 kV, ×100 000)"

Figure 4

Surface morphology observed by atomic force microscopy in two different scanning modes"

Figure 5

Circular dichroism spectra of calf thymus DNA incubated with Bioplatin A: Calf thymus DNA; B: Calf thymus DNA plus 20 μmol/L Bioplatin; C: Calf thymus DNA plus 40 μmol/L Bioplatin."

Figure 6

Fourier-transform infrared spectra of Bioplatin mixed with honey"

Figure 7

Cell viability of Bioplatin with different concentrations against PBMCs (A) and A375 cells (B) tested by MTT assay All results were expressed with vehicle control as 100%. Data are represented as mean ± standard deviation, n=3; *P<0.05, **P<0.01, vs control group. PBMC: peripheral blood mononuclear cell; MTT: 3-(4,5)-dimethylthiahiazo-(-z-y1)-3,5-di-phenytetrazoliumromide."

Figure 8

Fluorescence photomicrographs of A375 cells stained with DAPI A: Cells were stained with DAPI (a: Control; b to d: 24, 48, and 72 h after 100 μg/mL Bioplatin treatment). The cells were harvested at different time intervals and the blue fluorescence was monitored by fluorescence microscopy (×10). B: Histogram represents average fluorescence intensity of cells after DAPI staining. Data are represented as mean ± standard error, n=3; **P<0.01, vs control group. DAPI: 4′,6-diamidino-2-phenylindole."

Figure 9

Fluorescence photomicrographs of A375 cells stained with Hoechst 33258 A: Cells were stained with Hoechst 33258 (a: Control; b to d: 24, 48, and 72 h after 100 μg/mL Bioplatin treatment). The cells were harvested at different time intervals and the blue fluorescence was monitored by fluorescence microscopy (×10). B: Histogram represents average fluorescence intensity of cells after Hoechst 33258 staining. Data are represented as mean ± standard error, n=3; **P<0.01, vs control group."

Figure 10

DNA fragmentation after treatment of cells with Bioplatin in different time intervals A375 cells were treated with 100 μg/mL Bioplatin for 24, 48 and 72 h and Genomic DNA was extracted and DNA was separated on 1% agarose gels and stained with ethidium bromide. L1: Control DNA; L2: DNA from treated cells (24 h); L3: DNA from treated cells (48 h); L4: DNA from treated cells (72 h); L5: A standard DNA ladder."

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