枸杞多糖对荷瘤小鼠肿瘤微环境T淋巴细胞亚群及树突状细胞的影响

doi:10.3736/jcim20050511

Abstract

Objective

To study the effects of Lycium barbarum polysaccharide (LBP) on tumor microenvironment T-lymphocyte subsets and dendritic cells in H22-bearing mice and the mechanisms for intervention of tumor immune escape by LBP.

Methods

H22-bearing mice were given LBP orally for two weeks. T-lymphocyte subsets and the phenotypes of dendritic cells in tumor-infiltrating lymphocytes (TIL) were detected by flow cytometry (FCM).

Results

LBP could significantly increase the numbers of CD4 ⁺ and CD8 ⁺ T cells in TIL as compared with those in model control group (P<0.05). In model control group, the number of dendritic cells in tumor microenvironment decreased markedly, while in LBP-treated group, the increased number of dendritic cells and B7-1 expression were observed, but there were no significant differences between these two groups.

Conclusion

LBP has anti-tumor effect probably by increasing the numbers of CD4 ⁺ and CD8 ⁺ T cells in TIL to relieve the immunosuppression and enhance the anti-tumor function of the immune system. But whether LBP can recover the phenocyte and function of dendritic cells in H22-bearing mice should be further studied.

Key words: Lycium barbarum polysaccharide, Immunosuppression, T-lymphocyte subsets, Tumor-infiltrating lymphocyte, Dendritic cells

CLC Number:

R730.51

Yan-li He, Yi Ying, Yan-li Xu, Jun-fang Su, Hui Luo, Hui-feng Wang. Effects of Lycium barbarum polysaccharide on tumor microenvironment T-lymphocyte subsets and dendritic cells in H22-bearing mice. Journal of Chinese Integrative Medicine, 2005, 3(5): 374-377.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20050511

http://www.jcimjournal.com/EN/Y2005/V3/I5/374

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Group	n	Thymus weight (g)	Thymus index (×10^-3)
Normal	15	0.0 859±0.0 085	4.31±0.43
Model control	15	0.0 687±0.0 037	3.42±0.19
Low-dose LBP-treated	15	0.0 768±0.0 071	3.84±0.36
High-dose LBP-treated	15	0.0 817±0.0 091	4.02±0.46*

Group	n	CD4⁺ positive cells	CD8⁺ positive cells	CD11c⁺ positive cells	CD80⁺ positive cells	CD11c⁺and CD80⁺ double positive cells
Model control	10	12.89±1.05	7.72±0.48	23.07±2.36	15.15±1.47	11.65±1.73
Low-dose LBP-treated	8	16.95±1.81	13.20±0.67*	26.76±1.31	16.26±1.76	13.47±0.96
High-dose LBP-treated	8	23.39±2.43*	14.36±1.16*	27.61±2.56	18.75±0.92	13.61±1.88

Effects of Lycium barbarum polysaccharide on tumor microenvironment T-lymphocyte subsets and dendritic cells in H22-bearing mice

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