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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (5): 374-377.doi: 10.3736/jcim20050511

• Original Experimental Research • Previous Articles     Next Articles

Effects of Lycium barbarum polysaccharide on tumor microenvironment T-lymphocyte subsets and dendritic cells in H22-bearing mice

Yan-li He1,Yi Ying2,Yan-li Xu2,Jun-fang Su1,Hui Luo1,Hui-feng Wang1   

  1. 1. Department of Pathology, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong Province 510405, China
    2. Department of Hematology, Guangzhou Municipal First People’s Hospital, Guangzhou, Guangdong Province 510180, China;
  • Received:2004-12-19 Online:2005-09-20 Published:2005-09-20
  • Contact: Yan-li He


To study the effects of Lycium barbarum polysaccharide (LBP) on tumor microenvironment T-lymphocyte subsets and dendritic cells in H22-bearing mice and the mechanisms for intervention of tumor immune escape by LBP.


H22-bearing mice were given LBP orally for two weeks. T-lymphocyte subsets and the phenotypes of dendritic cells in tumor-infiltrating lymphocytes (TIL) were detected by flow cytometry (FCM).


LBP could significantly increase the numbers of CD4 + and CD8 + T cells in TIL as compared with those in model control group (P<0.05). In model control group, the number of dendritic cells in tumor microenvironment decreased markedly, while in LBP-treated group, the increased number of dendritic cells and B7-1 expression were observed, but there were no significant differences between these two groups. 


LBP has anti-tumor effect probably by increasing the numbers of CD4 + and CD8 + T cells in TIL to relieve the immunosuppression and enhance the anti-tumor function of the immune system. But whether LBP can recover the phenocyte and function of dendritic cells in H22-bearing mice should be further studied.

Key words: Lycium barbarum polysaccharide, Immunosuppression, T-lymphocyte subsets, Tumor-infiltrating lymphocyte, Dendritic cells

CLC Number: 

  • R730.51

Tab 1

Effect of LBP on thymus index in H22-bearing mice ($\bar{x}$±s )"

Group n Thymus weight
Thymus index
Normal 15 0.0 859±0.0 085 4.31±0.43
Model control 15 0.0 687±0.0 037 3.42±0.19
Low-dose LBP-treated 15 0.0 768±0.0 071 3.84±0.36
High-dose LBP-treated 15 0.0 817±0.0 091 4.02±0.46*

Tab 2

Effect of LBP on T-lymphocyte subsets and phenotypes of dendritic cells in TIL ($\bar{x}$±s, %)"

Group n CD4+
positive cells
positive cells
positive cells
positive cells
CD11c+and CD80+
double positive cells
Model control 10 12.89±1.05 7.72±0.48 23.07±2.36 15.15±1.47 11.65±1.73
Low-dose LBP-treated 8 16.95±1.81 13.20±0.67* 26.76±1.31 16.26±1.76 13.47±0.96
High-dose LBP-treated 8 23.39±2.43* 14.36±1.16* 27.61±2.56 18.75±0.92 13.61±1.88
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