SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (2): 143-147.doi: 10.3736/jcim20110205

• Original Clinical Research • Previous Articles     Next Articles

Correlation between T lymphocyte subsets and different syndrome types in patients with influenza A (H1N1): A retrospective study

Yu Huang1, Xing Zeng1, Wei Zhu2, Sha-sha Li1, Dan Zhou1, Chuan-jian Lu3()   

  1. 1. Central Laboratory, Guangdong Hospital of Traditional Chinese Medicine, Guangzhou 510006, Guangdong Province, China
    2. Laboratory of Chinese Drugs Pharmaceutics, Guangdong Hospital of Traditional Chinese Medicine, Guangzhou 510006, Guangdong Province, China
    3. Department of Dermatology, Guangdong Hospital of Traditional Chinese Medicine, Guangzhou 510006, Guangdong Province, China
  • Received:2010-09-17 Accepted:2010-11-02 Online:2011-02-20 Published:2011-02-15
  • Contact: Lu Chuan-jian E-mail:luchuanjian888@vip.sina.com

RichHTML

1

PDF (PC)

108

Like

Objective: To investigate the changes in T lymphocyte subsets in patients with different syndrome types infected by influenza A (H1N1) virus after treatment.
Methods: In this retrospective study, 111 patients with H1N1 influenza were enrolled and divided into three groups according to their syndromes: exterior heat syndrome group (55 cases), exterior cold syndrome group (20 cases) and heat toxin invading lung (HTIL) syndrome group (36 cases). Patients were treated with Western medicine (acetaminophen or ibuprofen suspension), traditional Chinese medicine (herbal medicine according to their syndromes), or a combination of Western medicine and traditional Chinese medicine with patient’s intentions. Another 20 healthy people were selected as normal control. Phenotypic features of T lymphocyte subsets in peripheral vein blood of patients before and after treatment were determined by flow cytometry.
Results: Before treatment, the numbers of CD3- and CD4-postive T lymphocytes were lower in H1N1 patients with the three types of syndromes than in the normal controls (P<0.01), and the numbers of CD8-positive T lymphocytes were lower in exterior heat syndrome and HTIL syndrome groups than in normal control group (P<0.01). The ratio of CD4-positive to CD8-positive T lymphocytes in exterior cold syndrome group showed no difference as compared with the normal control group, and the ratios of CD4-positive to CD8-positive T lymphocytes in exterior heat syndrome and HTIL syndrome groups were higher than that in the normal control group (P<0.01). After treatment, the numbers of CD4-positive T lymphocytes increased in all the three types of syndromes in patients with H1N1, and showed no difference compared with the normal control group. The numbers of CD3-positive T lymphocytes in patients with exterior heat syndrome and exterior cold syndrome increased, and showed no difference compared with the normal control group. The number of CD3-positive T lymphocytes in H1N1 patients with HTIL syndrome was lower than that in the normal control group (P<0.05), whereas the numbers of CD8-positive T lymphocytes in patients with exterior heat syndrome and HTIL syndrome were lower than that in the normal control group (P<0.05). The ratio of CD4-positive to CD8-positive T lymphocytes in patients with HTIL syndrome was decreased, and displayed no difference as compared with the normal control group. The ratio of CD4-positive to CD8-positive T lymphocytes in patients with exterior heat syndrome was higher than that in the normal control group (P<0.05).
Conclusion: Analysis of T lymphocyte subsets may provide experimental data to support for syndrome differentiation in traditional Chinese medicine and a sound method to establish syndrome differentiation typing for H1N1.

Key words: influenza A virus, H1N1 subtype, T lymphocyte subsets, syndrome, clinical trials, retrospective study

"

"

[1] Park AW, Daly JM, Lewis NS, Smith DJ, Wood JL, Grenfell BT . Quantifying the impact of immune escape on transmission dynamics of influenza[J]. Science, 2009,326(5953):726-728
doi: 10.1126/science.1175980 pmid: 3800096
[2] Madsen L, Nielsen A, Lundgren J . Naturally occurring oseltamivir resistance in influenza A[J]. Ugeskr Laeger, 2010,172(32):2165-2167
pmid: 20696118
[3] Langley WA, Bradley KC, Li ZN, Talekar GR, Galloway SE, Steinhauer DA . The effects of preexisting immunity to influenza on responses to influenza vectors in mice[J]. Vaccine, 2010,28(38):6305-6313
doi: 10.1016/j.vaccine.2010.06.112 pmid: 20656032
[4] Ministry of Health of the People’s Republic of China. Influenza A(H1N1) diagnosis and treatment program[M]. 2009
中华人民共和国卫生部. 甲型H1N1流感诊疗方案(2009年试行版第三版)[M]. 2009
[5] Adamson WE, Maddi S, Robertson C , McDonagh S , Molyneaux PJ, Templeton KE , Carman WF .2009 pandemic influenza A(H1N1) virus in Scotland: geographically variable immunity in Spring 2010, following the winter outbreak[J]. Euro Surveill, 2010,15(24):19590
[6] Zhang Q, Ling W, Li X, Lu LH, Chen ZH, Li XW . Changes of T lymphocyte subsets in sixty-seven patients with influenza A(H1N1)[J]. Zhongguo Quan Ke Yi Xue, 2010,13(6):571-573
张琦, 凌伟, 李鑫, 卢联合, 陈志海, 李兴旺 . 甲型H1N1流感患者T细胞亚群的变化特点(附67例报告)[J]. 中国全科医学, 2010,13(6):571-573
[7] McGill J, Van Rooijen N, Legge KL . IL-15 trans-presentation by pulmonary dendritic cells promotes effector CD8 T cell survival during influenza virus infection[J]. J Exp Med, 2010,207(3):521-534
doi: 10.1084/jem.20091711
[8] Keynan Y, Card CM, Ball BT, Li Y, Plummer FA, Fowke KR . Cellular immune responses to recurring influenza strains have limited boosting ability and limited cross-reactivity to other strains[J]. Clin Microbiol Infect, 2010,16(8):1179-1186
doi: 10.1111/j.1469-0691.2010.03142.x
[9] Zhang W, Jia XH, Zhao Y . Analysis of the correlation between pathogenic cold and meteorologic factors in chronic bronchitis[J]. Beijing Zhong Yi Yao Da Xue Xue Bao, 2004,27(1):77-79
doi: 10.3321/j.issn:1006-2157.2004.01.025
张伟, 贾新华, 赵燕 . 慢性支气管炎中寒邪与气象因子相关性分析[J]. 北京中医药大学学报, 2004,27(1):77-79
doi: 10.3321/j.issn:1006-2157.2004.01.025
[10] Zhang W, Liu HY . Difference between different grades of pathogenic cold and cytokines of rat[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2008,14(8):600-601
doi: 10.3969/j.issn.1006-3250.2008.08.016
张伟, 刘海瑜 . 不同梯度变化的寒邪对大鼠细胞因子影响之差异的实验研究[J]. 中国中医基础医学杂志, 2008,14(8):600-601
doi: 10.3969/j.issn.1006-3250.2008.08.016
[1] Zeinab Vafaeipour, Bibi Marjan Razavi, Hossein Hosseinzadeh. Effects of turmeric (Curcuma longa) and its constituent (curcumin) on the metabolic syndrome: An updated review. Journal of Integrative Medicine, 2022, 20(3): 193-203.
[2] Shu-yi Chen, Qun-wei Chen, Liu-mei Shou, Hong Pan, Shan-ming Ruan, Zhe-hao Liang, Qi-jin Shu. Stevens-Johnson syndrome/toxic epidermal necrolysis successfully treated with Chinese herbal medicine Pi-Yan-Ning: A case report. Journal of Integrative Medicine, 2021, 19(6): 555-560.
[3] Rui-jin Qiu, Min Li, Jia-yuan Hu, Jing Chen, Hong-cai Shang. Methods for development of a core outcome set for clinical trials integrating traditional Chinese medicine and Western medicine. Journal of Integrative Medicine, 2021, 19(5): 389-394.
[4] Liang Ding, Xin-you Zhang, Di-yao Wu, Meng-ling Liu. Application of an extreme learning machine network with particle swarm optimization in syndrome classification of primary liver cancer. Journal of Integrative Medicine, 2021, 19(5): 395-407.
[5] Si-qi Tang, Yun-liang Wang, Zi-ye Xie, Yang Zhang, Yi Guo, Kang-li Gao, Tang-you Mao, Chun-e Xie, Jun-xiang Li, Xiao-yan Gao. Serum metabolic profiling of traditional Chinese medicine syndromes in patients with diarrhea-predominant irritable bowel syndrome. Journal of Integrative Medicine, 2021, 19(3): 274-281.
[6] Shu-jun Wei, Qing-man He, Qing Zhang, Kang-hua Fu, Ruo-lan Li, Wei Peng, Yong-xiang Gao. Traditional Chinese medicine is a useful and promising alternative strategy for treatment of Sjogren's syndrome: A review. Journal of Integrative Medicine, 2021, 19(3): 191-202.
[7] Dastan M. Khoshnaw, Abhijit A. Ghadge. Yoga as a complementary therapy for metabolic syndrome: A narrative review. Journal of Integrative Medicine, 2021, 19(1): 6-12.
[8] Johannes Loeser, Brigitte Layer, Christopher Plata, Klaus-Maria Perrar, Tim Hucho, Rebecca Kulbida. Hirudotherapy attenuates arthritic pain in patients with various chronic pain syndromes: A retrospective analysis. Journal of Integrative Medicine, 2020, 18(5): 425-433.
[9] . External application of two unrestricted herbal medicines to treat costochondritis in a young collegiate athlete: A case report. Journal of Integrative Medicine, 2020, 18(5): 450-454.
[10] Shi-xin Wang, Yan Wang, Yu-bao Lu, Jie-yun Li, Yu-jun Song, Munkhtuya Nyamgerelt, Xue-xi Wang. Diagnosis and treatment of novel coronavirus infectious pneumonia based on the theory of traditional Chinese medicine. Journal of Integrative Medicine, 2020, 18(4): 275-283.
[11] Zi-jia Zhang, Wen-yong Wu, Jin-jun Hou, Lin-lin Zhang, Fei-fei Li, Lei Gao, Xing-dong Wu, Jing-ying Shi, Rong Zhang, Hua-li Long, Min Lei, Wan-ying Wu, De-an Guo, Kai-xian Chen, Lewis A. Hofmann, Zhonghua Ci. Active constituents and mechanisms of Respiratory Detox Shot, a traditional Chinese medicine prescription, for COVID-19 control and prevention: network-molecular docking-LC-MSE analysis. Journal of Integrative Medicine, 2020, 18(3): 229-241.
[12] Sherman Gu, Arthur Yin Fan. Controversial conclusions from two randomized controlled trials for acupuncture's effects on polycystic ovary syndrome or in vitro fertilization support. Journal of Integrative Medicine, 2020, 18(2): 89-91.
[13] Xue-qing Yu, Shu-guang Yang, Yang Xie, Jian-sheng Li. Traditional Chinese medicine in the treatment of idiopathic pulmonary fibrosis based on syndrome differentiation: Study protocol of exploratory trial. Journal of Integrative Medicine, 2020, 18(2): 163-168.
[14] Rebecca Kulbida, Alexander Mathes, Johannes Loeser. Beneficial effects of hirudotherapy in a chronic case of complex regional pain syndrome. Journal of Integrative Medicine, 2019, 17(5): 383-386.
[15] Arthur Yin Fan, Hui Ouyang, Xinru Qian, Hui Wei, David Dehui Wang, Deguang He, Haihe Tian, Changzhen Gong, Amy Matecki, Sarah Faggert Alemi. Discussions on real-world acupuncture treatments for chronic low-back pain in older adults. Journal of Integrative Medicine, 2019, 17(2): 71-76.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3