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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (10): 934-939.doi: 10.3736/jcim20091005

• Original Clinical Research • Previous Articles     Next Articles

Relationship between the phenotypes and functions of peripheral blood dendritic cells and the different spleen deficiency syndrome types in patients with chronic hepatitis B

 Lei Wanga, Xiao-xia Fengb,Wei Zhanga,Lian-jun Xingb,Pei-yong  Zhengb,Guang Jia   

  1. a Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    b Department of Liver Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2009-06-25 Accepted:2009-08-12 Online:2009-10-20 Published:2009-10-15
  • Contact: Lian-jun Xing


To study the phenotypes and functions of dendritic cells (DCs) derived from peripheral blood monocytes of chronic hepatitis B (CHB) patients with different traditional Chinese medicine (TCM) syndrome types, and to explore the relationship between TCM syndrome type and DC functions.

Sixty CHB patients were included in this study. All the CHB patients were divided into spleen deficiency and liver stagnation, spleen deficiency and dampness-heat and deficiency of both spleen and kidney groups according to TCM syndrome diagnosis standard. There were 20 cases in each group, and ten healthy people were included as normal control. The volunteer’s peripheral blood was collected for monocyte separation, biochemical test and hepatitis B virus DNA loads detection. DCs were induced and isolated from peripheral blood monocytes, and then the expressions of surface markers CD80, CD86, CD1a and HLA-DR were detected by flow cytometric analysis method. Interleukin-10 (IL-10) production of the DCs was quantified by enzyme-linked immunosorbent assay.

The proliferation of DCs in the CHB patients was slower than that in the healthy volunteers (P<0.05). The expressions of DC surface molecules such as CD80, CD86, and CD1a were obviously decreased in the CHB patients as compared with those in the healthy volunteers (P<0.05). More over, expressions of DC surface molecules were different among CHB patients with different TCM syndrome types. The positive expressions of CD80, CD1a, and HLA-DR in the CHB patients with spleen deficiency and liver stagnation were obviously higher than those in the CHB patients with deficiency of both spleen and kidney (P<0.05), and the CD1a expression in the CHB patients with spleen deficiency and dampness-heat was higher than that in the CHB patients with deficiency of both spleen and kidney (P<0.05). In DC culture supernatant, the IL-10 concentration of the CHB patients with deficiency of both spleen and kidney was higher than that of the CHB patients with spleen deficiency and liver stagnation (P<0.05), and the IL-10 concentrations of the CHB patients with different TCM syndrome types were higher than that of the healthy volunteers (P<0.05).

During the pathogenic course of CHB, the phenotypes and functions of DCs are different in CHB patients with different TCM syndrome types. It suggests that there is a correlation between TCM syndrome type and body immunity function.

Key words: Chronic hepatitis B, Dendritic cell, Traditional Chinese medicine, Spleen deficiency, Syndrome

Figure 1

Morphology of DC at different time points after adherent cultivation (Inverted phase contrast microscopy, ×200) A: Two hours after adherent cultivation; B: Day three after adherent cultivation; C: Day five after adherent cultivation; D: Day seven after adherent cultivation."

Figure 2

Morphology of mature DC at day seven after adherent cultivation (Electronmicroscopy, ×3 000)"

Table 1

Biochemistry and HBV-DNA loads of CHB patients with different TCM syndrome type"

Group n ALT
($\bar{x}$±s, U/L)
($\bar{x}$±s, U/L)
($\bar{x}$±s, μmol/L)
[Median (Q25, Q75), IU/mL]
Spleen deficiency and liver stagnation 20 35.81±20.74 51.44±32.34 14.78±4.49 1.42×105 (6.04×104, 1.75×107)
Spleen deficiency and dampness-heat 20 61.06±66.75 65.14±47.33 16.54±5.85 4.19×106 (2.16×105, 1.52×107)
Deficiency of spleen and kidney 20 34.94±17.51 41.38±23.19 16.35±6.29 9.67×105 (9.77×104, 8.99×107)

Table 2

Expressions of DC surface markers in CHB patients with different TCM syndrome type ($\bar{x}$±s, %)"

Group n CD80 CD86 CD1a HLA-DR
Normal control 10 64.72±9.85 75.10±7.76 62.77±5.57 59.00±7.94
Spleen deficiency and liver stagnation 20 11.84±11.14* 54.51±12.14* 32.01±11.17* 51.42±12.09
Spleen deficiency and dampness-heat 20 9.00±7.55* 53.98±13.60* 29.30±11.28* 46.09±11.77*
Deficiency of spleen and kidney 20 5.94±2.62* 42.64±22.05* 22.14±10.37*△▲ 39.29±15.52*

Figure 3

Expressions of DC surface markers in CHB patients with different TCM syndrome type A: Normal control group; B: Spleen deficiency and liver stagnation group; C: Spleen deficiency and dampness-heat group; D: Deficiency of spleen and kidney group."

[1] Tang YP, Shao Y . Dendritic cell and hepatitis B infection[J]. Lin Chuang Hui Cui, 2004,19(4):232-234
doi: 10.3969/j.issn.1004-583X.2004.04.038
唐艳萍, 邵沂 . 树突状细胞与乙肝病毒感染[J]. 临床荟萃, 2004,19(4):232-234
doi: 10.3969/j.issn.1004-583X.2004.04.038
[2] Sprengers D, van der Molen RG, Kusters JG, Hansen B, Niesters HG, Schalm SW, Janssen HL . Different composition of intrahepatic lymphocytes in the immune-tolerance and immune-clearance phase of chronic hepatitis B[J]. J Med Virol, 2006,78(5):561-568
doi: 10.1002/(ISSN)1096-9071
[3] Gao YQ, Zheng YJ, Wang LT . Effect of spleen-invigorating prescription on dendritic cell function in patients with chronic hepatitis B of TCM pi-deficiency syndrome type[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2007,27(4):300-302
doi: 10.3321/j.issn:1003-5370.2007.04.005
高月求, 郑亚江, 王灵台 . 健脾方对脾虚型慢性乙型肝炎患者树突细胞功能的影响[J]. 中国中西医结合杂志, 2007,27(4):300-302
doi: 10.3321/j.issn:1003-5370.2007.04.005
[4] Chinese Society of Infectious Diseases and Parasitology and Chinese Society of Hepatology of Chinese Medical Association. Prevention and treatment program for viral hepatitis[J]. Gan Zang, 2000,5(4):257-263
中华医学会传染病与寄生虫病学分会, 肝病学分会. 病毒性肝炎防治方案[J]. 肝脏, 2000,5(4):257-263
[5] Ministry of Health of the People’s Republic of China. Guidelines for clinical research on Chinese new herbal medicines[M]. Beijing: Medical Science and Technology Publishing House of China, 1993
中华人民共和国卫生部. 中药新药临床研究指导原则[M]. 北京: 中国医药科技出版社, 1993
[6] Deng TT, Guo ZQ. Diagnostics of traditional Chinese medicine(5th ed)[M]. Shanghai: Shanghai Scientific and Technical Publishers, 1984,( 5th ed)
邓铁涛, 郭振球 . 中医诊断学(第5版)[M]. 上海: 上海科学技术出版社, 1984,( 第5版)
[7] Chen MQ, Shi GF, Lu Q, Li Q, Zhang QH, Qin G, Weng XH . Phenotypes and functions of dendritic cells derived from peripheral blood monocytes of chronic hepatitis B patients with different HBV DNA loads[J]. Zhonghua Gan Zang Bing Za Zhi, 2007,15(1):19-24
doi: 10.3760/j.issn:1007-3418.2007.01.006
陈明泉, 施光峰, 卢清, 李谦, 张琼华, 秦刚, 翁心华 . 不同病毒载量的慢性乙型肝炎患者外周血树突状细胞的表型和功能[J]. 中华肝脏病杂志, 2007,15(1):19-24
doi: 10.3760/j.issn:1007-3418.2007.01.006
[8] Yu YS, Tang ZH , Zang GQ.The function of immuno-modulating on CDla dendritic cells by IFN-α in chronic hepatitis B patients in vivo[J]. Zhonghua Gan Zang Bing Za Zhi, 2005, 13(1): 838, 843
doi: 10.3760/j.issn:1007-3418.2005.11.016
余永胜, 汤正好, 臧国庆 . 体内观察干扰素α对慢性乙型肝炎患者CD1a树突状细胞的免疫调节作用[J].中华肝脏病杂志, 2005, 13(1): 838, 843
doi: 10.3760/j.issn:1007-3418.2005.11.016
[9] Atassi MZ, Casali P . Molecular mechanisms of autoimmunity[J]. Autoimmunity, 2008,41(2):123-132
doi: 10.1080/08916930801929021
[10] Zammit DJ, Lefrancois L . Dendritic cell-T cell interactions in the generation and maintenance of CD8 T cell memory[J]. Microbes Infect, 2006,8(4):1108-1115
doi: 10.1016/j.micinf.2005.12.002 pmid: 16549379
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