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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (12): 1482-1489.doi: 10.3736/jcim20121223

• Original Experimental Research • Previous Articles     Next Articles

Effects of naringenin on allodynia and hyperalgesia in rats with chronic constriction injury-induced neuropathic pain

Kaulaskar Shyam, Bhutada Pravinkumar(), Rahigude Anand, Jain Dilpesh, Harle Uday   

  1. Post-Graduate Research Department, Sinhgad College of Pharmacy, Pune 411041, Maharashtra, IndiaPost-Graduate Research Department, Sinhgad College of Pharmacy, Pune 411041, Maharashtra, India
  • Received:2012-08-03 Accepted:2012-10-28 Online:2012-12-20 Published:2018-12-15

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Objective: To study the analgesic effects of naringenin on chronic constriction injury (CCI) model of neuropathic pain.
Methods: After inducing of neuropathic pain by CCI, treatment with 25 and 50 mg/kg of naringenin and 10 mg/kg of pregabalin was given. Rats were evaluated for behavioral tests using Hargreaves apparatus for thermal hyperalgesia, pin prick test for tactile mechanical hyperalgesia and cold water-induced allodynia on days 0, 3, 5, 7, 14 and 21. At the end of study, oxidative stress parameters were measured.
Results: Naringenin showed ameliorating action against CCI-induced neuropathic pain in all the tested models. Also, naringenin attenuated the elevated levels of lipid peroxidation and nitric oxide, and restored the level of reduced glutathione.
Conclusion: The results of the present investigation suggest that naringenin exhibits analgesic effect in sciatic nerve injury model.

Key words: analgesics, sciatica, hyperalgesia, flavonoids, pain, oxidative stress

Figure 1

Effect of naringenin on CCI-induced neuropathic pain in thermal hyperalgesia model Data are expressed as mean±standard error of mean, and repeated measure of two-way analysis of variance followed by Bonferroni’s post test was used for the statistical analysis; n=6. **P<0.01, vs sham-operated group; △P<0.05,△△P<0.01, vs CCI control group. CCI: chronic constriction injury."

Figure 2

Effect of naringenin on CCI-induced neuropathic pain in cold allodynia model Data are expressed as mean±standard error of mean, and results obtained were analyzed by repeated two-way analysis of variance followed by Bonferroni’s post test, n=6. **P<0.01, vs sham-operated group; △P<0.05, △△P<0.01, vs CCI control group. CCI: chronic constriction injury."

Figure 3

Effect of naringenin on CCI-induced neuropathic pain in mechanical hyperalgesia model Data are expressed as mean±standard error of mean, and results obtained were analyzed by repeated two-way analysis of variance followed by Bonferroni’s post test. **P<0.01, vs sham-operated group; △△P<0.01, vs CCI control group. CCI: chronic constriction injury."

Figure 4

Effect of naringenin on CCI-induced neuropathic pain in exploratory behavior (Locomotor activity) test Data are expressed as mean±standard error of mean. Repeated measure of two-way analysis of variance followed by Bonferronis post test."

Figure 5

Effect of naringenin on oxidative stress markers in CCI-induced neuropathic pain model A: effect of naringenin on MDA level; B: effect of naringenin on GSH level; C: effect of naringenin on NO level. Data are expressed as mean±standard mean of error, and results obtained were analyzed by one-way analysis of variance followed by Tukey’s multiple comparison, n=6. **P<0.01, vs sham-operated group, △△P<0.01, vs CCI control group. MDA: malondialdehyde; GSH: reduced glutathione; NO: nitric oxide; CCI: chronic constriction injury."

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