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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (3): 330-336.doi: 10.3736/jcim20120313

• Original Experimental Research • Previous Articles     Next Articles

Effects of a compound Chinese medicine Xinji’erkang on isoproterenol-induced ventricular remodeling in mice

Gao Shan1,Wang Xing-hui1,Huang Ling-ling1,Yu Ting-ting1,Du Su-ming1,Guo Yan-wei1,Jia Yuan1,Wang Jian2()   

  1. 1. Department of Pharmacology, College of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China
    2. Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China
  • Received:2011-09-21 Accepted:2011-11-09 Online:2012-03-20 Published:2018-10-10

OBJECTIVE: To investigate the effects of Xinji’erkang (XJEK), a compound Chinese herbal medicine, on isoproterenol-induced ventricular remodeling in mice.
METHODS: Isoproterenol was given subcutaneously (1 mg/kg, twice per day for 7 d) to induce ventricular remodeling in mice. Mice were divided into normal control group, model group, XJEK low-, medium- and high-dose groups, XJEK water layer group, XJEK n-butanol layer group and metoprolol group. All drugs were given by intragastric administration. At the end of the 7th day, the hearts of the rats were weighted, and myocardial hypertrophy index was expressed as heart weight/body weight (HW/BW). The histological changes were observed by hemotoxylin-eosin and Van Gieson staining. Colorimetric method was used to determine the content of hydroxyproline in heart, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in serum.
RESULTS: Compared with the isoproterenol injection only, XJEK potently inhibited cardiomyocyte hypertrophy and the increase of hydroxyproline content in heart (P<0.01), improved cardiac pathology change, inhibited the decrease of SOD activity and the increase of MDA content in serum (P<0.01). XJEK water layer also inhibited the increase of cardiomyocyte hypertrophy (P<0.01) while XJEK n-butanol layer inhibited cardiomyocyte hypertrophy and fibrosis (P<0.01).
CONCLUSION: XJEK possesses protective effects against isoproterenol-induced ventricular remodeling in mice, which may be related to its actions in reducing the oxidative stress and improving the antioxidant activity of the body. XJEK water layer and XJEK n-butanol layer attenuated ventricular remodeling without significant oxidative stress state changing, which indicates that a non-antioxidative stress mechanism may exist.

Key words: compounds, traditional Chinese drugs, isoproterenol, ventricular remodeling, oxdative stress, superoxide dismutase, malonyldialdehyde, mice

Table 1

Effects of XJEK on heart weight index in mice with isoprenaline-induced ventricular remodeling (x±s)"

Group n BW (g) HW (mg) HW/BW (mg/g)
Normal control 9 20.94±2.13 82.33±9.62 3.93±0.24
Model control 12 21.75±2.56 107.83±9.21 4.92±0.24**
XJEK low-dose 10 22.60±3.05 102.90±13.93 4.48±0.35△△
XJEK medium-dose 10 23.45±3.40 108.00±15.89 4.50±0.31△△
XJEK high-dose 10 24.30±3.29 105.20±14.73 4.34±0.40△△
XJEK water layer 11 23.82±1.82 108.36±15.04 4.54±0.35
XJEK n-butanol layer 10 23.50±1.27 103.30±9.93 4.33±0.32△△
Metoprolol 9 23.94±2.43 103.22±11.29 4.31±0.24△△

Table 2

Effects of XJEK on CSA, CVF, PVCA and hydroxyproline content in heart tissue of mice with isoprenaline-induced ventricular remodeling (x±s)"

Group n CSA (arbitrary unit) CVF (%) PVCA (%) Hyp (μg/mg heart tissue)
Normal control 9 244.11±22.90 1.80±0.62 2.85±0.52 0.23±0.05
Model control 12 307.11±33.48** 5.89±0.85** 8.79±1.53** 0.27±0.06*
XJEK low-dose 10 292.96±20.06 5.11±0.71 7.65±0.95 0.24±0.05
XJEK medium-dose 10 290.18±26.23 5.26±0.78 7.56±0.97 0.23±0.03
XJEK high-dose 10 274.14±16.45△△ 3.47±0.75△△ 4.98±0.89△△ 0.21±0.03
XJEK water layer 11 229.14±32.38 5.28±0.86 7.82±1.00 0.25±0.04
XJEK n-butanol layer 10 262.04±20.69△△ 3.31±0.63△△ 5.21±0.65△△ 0.26±0.04
Metoprolol 9 249.19±20.16△△ 2.83±0.66△△ 4.41±0.83△△ 0.20±0.06

Figure 1

Effects of Xinji’erkang on CSA in mice with isoprenaline-induced ventricular remodeling tested by hematoxylin-eosin staining (Light microscopy, ×400) A: Myocardial interstitial fibrosis; B: Myocardial perivascular fibrosis. a: Normal control; b: Model control; c: XJEK low-dose; d: XJEK medium-dose; e: XJEK high-dose; f: XJEK water layer; g: XJEK n-butanol layer: h: Metoprolol. CSA: cross section area; XJEK: Xinji’erkang."

Figure 2

Effects of XJEK on CVF and PVCA in mice with isoprenaline-induced ventricular remodeling tested by Van Gieson staining (Light microscopy, ×400) A: Myocardial interstitial fibrosis; B: Myocardial perivascular fibrosis. a: Normal control; b: Model control; c: XJEK low-dose; d: XJEK medium-dose; e: XJEK high-dose; f: XJEK water layer; g: XJEK n-butanol layer: h: Metoprolol. XJEK: Xinji’erkang; CVF: collagen volume fraction; PVCA: perivascular circumferential collagen area."

Table 3

Effects of XJEK on serum SOD activity and MDA content in mice with isoprenaline-induced ventricular remodeling (x±s)"

Group n SOD (kU/L) MDA (μmol/L)
Normal control 9 104.42±8.31 5.26±0.62
Model control 12 94.10±5.26** 6.30±0.51**
XJEK low-dose 10 98.19±6.21 5.98±0.39
XJEK medium-dose 10 100.74±6.11△△ 5.66±0.46△△
XJEK high-dose 10 101.51±4.97△△ 5.74±0.41△△
XJEK water layer 11 96.02±6.55 5.97±0.35
XJEK n-butanol layer 10 95.39±5.23 6.05±0.43
Metoprolol 9 101.36±4.88△△ 5.55±0.43△△
[1] Takeshita D, Shimizu J, Kitagawa Y, Yamashita D, Tohne K, Nakajima-Takenaka C, Ito H, Takaki M . Isoproterenol-induced hypertrophied rat hearts : does short-term treatment correspond to long-term treatment? J Physiol Sci. 2008; 58(3):179-188.
doi: 10.2170/physiolsci.RP004508
[2] Wang J, Cao EZ , Li LZ. Xin Ji Er Kang in coronary heart disease. Nanjing Zhong Yi Yao Da Xue Xue Bao. 1998; 14(4) : 201-212. Chinese with abstract in English.
王键, 曹恩泽, 李立志 . 心肌尔康治疗胸痹的临床观察.南京中医药大学学报. 1998; 14(4):210-212.
[3] Wang YJ, Jiang AH, Wang QM, Chen GL . Influences of Xinji Erkang granule on the activities of myocardium enzyme, superoxide dismutase and the content of malonyldialdehyde in tissue of mice with adriamycin- induced toxic myocarditis. Anhui Zhong Yi Xue Yuan Xue Bao. 2001 ; 20(3) : 43-45. Chinese with abstract in English.
汪远金, 江安宏, 王钦茂, 陈光亮 . 心肌尔康冲剂对阿霉素致中毒性心肌炎小鼠心肌酶和MDA含量、SOD活性影响.安徽中医学院学报. 2001; 20(3):43-45.
[4] Lin Y, Wang LN, Xi YH, Li HZ, Xiao FG, Zhao YJ, Tian Y, Yang BF, Xu CQ . L-arginine inhibits isoproterenol- induced cardiac hypertrophy through nitric oxide and polyamine pathways. Basic Clin Pharmacol Toxicol. 2008; 103(2):124-130.
doi: 10.1111/pto.2008.103.issue-2
[5] Shi H, Cheng LY, Ye YP, Li XY, Chen FY, Zheng XL, Zhang HY . Extraction and pharmacodynamic screening on the effective fractions of Artemisia capillaries Thumb. Zhejiang Sheng Yi Xue Ke Xue Yuan Xue Bao.Chinese with abstract in English.
史红, 程丽艳, 叶益萍, 李晓誉, 陈峰阳, 郑晓亮, 张慧阳 . “茵陈蒿汤”复方有效部位群的提取及药效筛选. 浙江省医学科学院学报. 2007; 71:19-24.
[6] Zuo YM, Gao S, Cao JF, Liu XY, Yu HJ , Zhang Y. Effects of oligomeric grape seed proanthocyanidins on isoproterenol-induced cardiac remodeling in rats. Yao Xue Xue Bao. 2010 ; 45 (5): 565-570. Chinese with abstract in English.
左友梅, 髙杉, 曹建飞, 刘晓雨, 於洪建, 张野 . 低聚葡萄籽原花青素对异丙肾上腺素诱导大鼠心室重构的影响.药学学报. 2010; 45(5):565-570.
[7] Gao S, Long CL, Wang RH, Wang H . KAtp activation prevents progression of cardiac hypertrophy to failure induced by pressure overload via protecting endothelial function. Cardiovase Res. 2009; 83(3):444-456
doi: 10.1093/cvr/cvp099
[8] Keles MS, Bayir Y, Suleyman H, Halici Z . Investigation of effects of Lacidipine, Ramipril and Valsartan on DNA damage and oxidative stress occurred in acute and chronic periods following isoproterenol-induced myocardial infarct in rats. Mol Cell Biochem. 2009; 328(1-2):109-117.
doi: 10.1007/s11010-009-0080-y
[9] Zhang GX, Ohmori K, Nagai Y, Fujisawa Y, Nishiyama A, Abe Y, Kimura S . Role of AT 1 receptor in isoproterenol- induced cardiac hypertrophy and oxidative stress in mice. J Mol Cell Cardiol. 2007 ; 42(4) : 804-811.
doi: 10.1016/j.yjmcc.2007.01.012
[10] Deng CQ . Thinking and practice on research of effective components of traditional Chinese medicine compound prescriptions. J Chin Integr Med. 2010 ; 8(12) : 1107- 1113. Chinese with abstract in English.
邓常青 . 中药复方有效组分研究的思路和实践.中西医结合学报. 2010; 8(12):1107-1113.
[11] Wang J, Liu CM, Bai HL, Li L . The antioxidative activity evaluations of the saponins in traditional Chinese medicine. Shizhen Guo Yi Guo Yao. 2010 ; 21(6):1485-1487.
王晶, 刘春明, 白鹤龙, 李丽 . 中药中皂苷类化合物的抗氧化活性评价研究.时珍国医国药. 2010; 21(6):1485-1487.
[12] Hu XY, Sun AS, Yu LM, Wu Q . Effects of Corydalis ambailismigo total alkaloids on experimental cerebral ischemia. J Chin Integr Med. 2005; 3(1) : 46-49. Chinese with abstract in English.
胡雪勇, 孙安盛, 余丽梅, 吴芹 . 夏天无总碱抗实验性脑缺血的作用.中西医结合学报. 2005; 3(1):46-49.
[13] Wu KQ, Zhao HX , Nie TT. Research progress on treating cardiovascular diseases with saponins in traditional Chinese medicine. Qi Lu Yao Shi. 2009 ; 28(9) : 547- 548. Chinese with abstract in English.
吴克芹, 赵焕新, 聂田田 . 中药皂苷治疗心血管疾病的研究进展.齐鲁药事. 2009; 28(9):547-548.
[14] Doggrell SA, Brown L . Rat models of hypertension, cardiac hypertrophy and failure. Cardiovasc Res. 1998; 39(1) : 89-105.
doi: 10.1016/S0008-6363(98)00076-5
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