心肌尔康对异丙肾上腺素致小鼠心室重构的保护作用及其机制

doi:10.3736/jcim20120313

Abstract

OBJECTIVE: To investigate the effects of Xinji’erkang (XJEK), a compound Chinese herbal medicine, on isoproterenol-induced ventricular remodeling in mice.
METHODS: Isoproterenol was given subcutaneously (1 mg/kg, twice per day for 7 d) to induce ventricular remodeling in mice. Mice were divided into normal control group, model group, XJEK low-, medium- and high-dose groups, XJEK water layer group, XJEK n-butanol layer group and metoprolol group. All drugs were given by intragastric administration. At the end of the 7th day, the hearts of the rats were weighted, and myocardial hypertrophy index was expressed as heart weight/body weight (HW/BW). The histological changes were observed by hemotoxylin-eosin and Van Gieson staining. Colorimetric method was used to determine the content of hydroxyproline in heart, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in serum.
RESULTS: Compared with the isoproterenol injection only, XJEK potently inhibited cardiomyocyte hypertrophy and the increase of hydroxyproline content in heart (P<0.01), improved cardiac pathology change, inhibited the decrease of SOD activity and the increase of MDA content in serum (P<0.01). XJEK water layer also inhibited the increase of cardiomyocyte hypertrophy (P<0.01) while XJEK n-butanol layer inhibited cardiomyocyte hypertrophy and fibrosis (P<0.01).
CONCLUSION: XJEK possesses protective effects against isoproterenol-induced ventricular remodeling in mice, which may be related to its actions in reducing the oxidative stress and improving the antioxidant activity of the body. XJEK water layer and XJEK n-butanol layer attenuated ventricular remodeling without significant oxidative stress state changing, which indicates that a non-antioxidative stress mechanism may exist.

Key words: compounds, traditional Chinese drugs, isoproterenol, ventricular remodeling, oxdative stress, superoxide dismutase, malonyldialdehyde, mice

Gao Shan, Wang Xing-hui, Huang Ling-ling, Yu Ting-ting, Du Su-ming, Guo Yan-wei, Jia Yuan, Wang Jian. Effects of a compound Chinese medicine Xinji’erkang on isoproterenol-induced ventricular remodeling in mice. Journal of Chinese Integrative Medicine, 2012, 10(3): 330-336.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20120313

http://www.jcimjournal.com/EN/Y2012/V10/I3/330

TrendMD

Figures/Tables 5

Table 1

Table 2

Figure 1

Figure 2

Table 3

References 14

[1]	Takeshita D, Shimizu J, Kitagawa Y, Yamashita D, Tohne K, Nakajima-Takenaka C, Ito H, Takaki M . Isoproterenol-induced hypertrophied rat hearts : does short-term treatment correspond to long-term treatment? J Physiol Sci. 2008; 58(3):179-188. doi: 10.2170/physiolsci.RP004508
[2]	Wang J, Cao EZ , Li LZ. Xin Ji Er Kang in coronary heart disease. Nanjing Zhong Yi Yao Da Xue Xue Bao. 1998; 14(4) : 201-212. Chinese with abstract in English.
	王键, 曹恩泽, 李立志 . 心肌尔康治疗胸痹的临床观察.南京中医药大学学报. 1998; 14(4):210-212.
[3]	Wang YJ, Jiang AH, Wang QM, Chen GL . Influences of Xinji Erkang granule on the activities of myocardium enzyme, superoxide dismutase and the content of malonyldialdehyde in tissue of mice with adriamycin- induced toxic myocarditis. Anhui Zhong Yi Xue Yuan Xue Bao. 2001 ; 20(3) : 43-45. Chinese with abstract in English.
	汪远金, 江安宏, 王钦茂, 陈光亮 . 心肌尔康冲剂对阿霉素致中毒性心肌炎小鼠心肌酶和MDA含量、SOD活性影响.安徽中医学院学报. 2001; 20(3):43-45.
[4]	Lin Y, Wang LN, Xi YH, Li HZ, Xiao FG, Zhao YJ, Tian Y, Yang BF, Xu CQ . L-arginine inhibits isoproterenol- induced cardiac hypertrophy through nitric oxide and polyamine pathways. Basic Clin Pharmacol Toxicol. 2008; 103(2):124-130. doi: 10.1111/pto.2008.103.issue-2
[5]	Shi H, Cheng LY, Ye YP, Li XY, Chen FY, Zheng XL, Zhang HY . Extraction and pharmacodynamic screening on the effective fractions of Artemisia capillaries Thumb. Zhejiang Sheng Yi Xue Ke Xue Yuan Xue Bao.Chinese with abstract in English.
	史红, 程丽艳, 叶益萍, 李晓誉, 陈峰阳, 郑晓亮, 张慧阳 . “茵陈蒿汤”复方有效部位群的提取及药效筛选. 浙江省医学科学院学报. 2007; 71:19-24.
[6]	Zuo YM, Gao S, Cao JF, Liu XY, Yu HJ , Zhang Y. Effects of oligomeric grape seed proanthocyanidins on isoproterenol-induced cardiac remodeling in rats. Yao Xue Xue Bao. 2010 ; 45 (5): 565-570. Chinese with abstract in English.
	左友梅, 髙杉, 曹建飞, 刘晓雨, 於洪建, 张野 . 低聚葡萄籽原花青素对异丙肾上腺素诱导大鼠心室重构的影响.药学学报. 2010; 45(5):565-570.
[7]	Gao S, Long CL, Wang RH, Wang H . K_Atp activation prevents progression of cardiac hypertrophy to failure induced by pressure overload via protecting endothelial function. Cardiovase Res. 2009; 83(3):444-456 doi: 10.1093/cvr/cvp099
[8]	Keles MS, Bayir Y, Suleyman H, Halici Z . Investigation of effects of Lacidipine, Ramipril and Valsartan on DNA damage and oxidative stress occurred in acute and chronic periods following isoproterenol-induced myocardial infarct in rats. Mol Cell Biochem. 2009; 328(1-2):109-117. doi: 10.1007/s11010-009-0080-y
[9]	Zhang GX, Ohmori K, Nagai Y, Fujisawa Y, Nishiyama A, Abe Y, Kimura S . Role of AT 1 receptor in isoproterenol- induced cardiac hypertrophy and oxidative stress in mice. J Mol Cell Cardiol. 2007 ; 42(4) : 804-811. doi: 10.1016/j.yjmcc.2007.01.012
[10]	Deng CQ . Thinking and practice on research of effective components of traditional Chinese medicine compound prescriptions. J Chin Integr Med. 2010 ; 8(12) : 1107- 1113. Chinese with abstract in English.
	邓常青 . 中药复方有效组分研究的思路和实践.中西医结合学报. 2010; 8(12):1107-1113.
[11]	Wang J, Liu CM, Bai HL, Li L . The antioxidative activity evaluations of the saponins in traditional Chinese medicine. Shizhen Guo Yi Guo Yao. 2010 ; 21(6):1485-1487.
	王晶, 刘春明, 白鹤龙, 李丽 . 中药中皂苷类化合物的抗氧化活性评价研究.时珍国医国药. 2010; 21(6):1485-1487.
[12]	Hu XY, Sun AS, Yu LM, Wu Q . Effects of Corydalis ambailismigo total alkaloids on experimental cerebral ischemia. J Chin Integr Med. 2005; 3(1) : 46-49. Chinese with abstract in English.
	胡雪勇, 孙安盛, 余丽梅, 吴芹 . 夏天无总碱抗实验性脑缺血的作用.中西医结合学报. 2005; 3(1):46-49.
[13]	Wu KQ, Zhao HX , Nie TT. Research progress on treating cardiovascular diseases with saponins in traditional Chinese medicine. Qi Lu Yao Shi. 2009 ; 28(9) : 547- 548. Chinese with abstract in English.
	吴克芹, 赵焕新, 聂田田 . 中药皂苷治疗心血管疾病的研究进展.齐鲁药事. 2009; 28(9):547-548.
[14]	Doggrell SA, Brown L . Rat models of hypertension, cardiac hypertrophy and failure. Cardiovasc Res. 1998; 39(1) : 89-105. doi: 10.1016/S0008-6363(98)00076-5

Related Articles 15

[1]	Victor Abou Nehmi Filho, Gilson Masahiro Murata, Ruan Carlos Macêdo de Moraes, Gabriely Cristina Alves Lima, Danielle Araujo de Miranda, Katrin Radloff, Raquel Galvão Figuerêdo Costa, Joyce de Cassia Rosa de Jesus, Jéssica Alves De Freitas, Nayara Izabel Viana, Ruan Pimenta, Katia Ramos Moreira Leite, José Pinhata Otoch, Ana Flávia Marçal Pessoa. A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice. Journal of Integrative Medicine, 2021, 19(5): 439-450.
[2]	Hao Gou, Ruo-chen Huang, Yong-hua Su, Wei Li. Design of dual targeting immunomicelles loaded with bufalin and study of their anti-tumor effect on liver cancer. Journal of Integrative Medicine, 2021, 19(5): 408-417.
[3]	Yi Ran Hu, San Li Xing, Chuan Chen, Ding Zhu Shen, Jiu Lin Chen. Tiaoxin Recipe, a Chinese herbal formula, inhibits microRNA-34a expression in the APPswe/PS1ΔE9 mouse model of Alzheimer's disease. Journal of Integrative Medicine, 2019, 17(6): 404-409.
[4]	Bhesh Raj Sharma, Dong Wook Kim, Dong Young Rhyu. Korean Chungtaejeon tea extract attenuates weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2017, 15(1): 56-63.
[5]	Roslinah Mohamad Hussain, Noor Faradilla Abdullah, Zulkhairi Amom. Killing of Staphylococcus aureus by allylpyrocatechol is potentiated by induction of intracellular oxidative stress and inhibition of catalase activity. Journal of Integrative Medicine, 2016, 14(6): 456-464.
[6]	Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[7]	Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[8]	Md. Asadujjaman, Ahmed Ullah Mishuk, Md. Aslam Hossain, Utpal Kumar Karmakar. Medicinal potential of Passiflora foetida L. plant extracts: Biological and pharmacological activities. Journal of Integrative Medicine, 2014, 12(2): 121-126.
[9]	Marjan Nassiri-Asl, Taghi Naserpour Farivar, Esmail Abbasi, Hamid Reza Sadeghnia, Mehdi Sheikhi, Mina Lotfizadeh, Parisa Bazahang. Effects of rutin on oxidative stress in mice with kainic acid-induced seizure. Journal of Chinese Integrative Medicine, 2013, 11(5): 337-342.
[10]	Md. Khirul Islam, Imran Mahmud, Sanjib Saha, Asit Baron Sarker, Himangsu Mondal, A.S.M. Monjur-Al-Hossain, Md. Anisuzzman. Preliminary pharmacological evaluation of Alocasia indica Schott tuber. Journal of Chinese Integrative Medicine, 2013, 11(5): 343-351.
[11]	Sanjib Saha, Faroque Hossain, Md. Anisuzzman, Md. Khirul Islam. Pharmacological evaluation of Musa seminifera Lour. Fruit. Journal of Chinese Integrative Medicine, 2013, 11(4): 253-261.
[12]	Sunil A. Nirmal, Jayashri M. Ingale, Shashikant R. Pattan, Sanjay B. Bhawar. Amaranthus roxburghianus root extract in combination with piperine as a potential treatment of ulcerative colitis in mice. Journal of Chinese Integrative Medicine, 2013, 11(3): 206-212.
[13]	Mei-jiao Mao, Jun-ping Hu, Cong Wang, Yi-yi Zhang, Ping Liu. Effects of Chinese herbal medicine Guanxinkang on expression of PPARγ-LXRα-ABCA1 pathway in ApoE-knockout mice with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(7): 814-820.
[14]	Lin Wang, Guan-liang Wang, Jia-han Liu, Di Li, De-zeng Zhu, Liang-neng Wu . Effects of ursolic acid in ameliorating insulin resistance in liver of KKAy mice via peroxisome proliferator-activated receptors α and γ. Journal of Chinese Integrative Medicine, 2012, 10(7): 793-799.
[15]	Yi-tao Zhao , Xin-guang Zhang , Li Bai , Li-qing Li , Jian-er Yu . Effects of Chinese herbal medicine Pingchuan Formula on airway inflammation, interferon-γ and interleukin-4 in mice with asthma. Journal of Chinese Integrative Medicine, 2012, 10(7): 807-813.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2]	Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3]	Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4]	Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5]	. Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6]	Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7]	Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8]	Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9]	Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10]	Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228

Group	n	BW (g)	HW (mg)	HW/BW (mg/g)
Normal control	9	20.94±2.13	82.33±9.62	3.93±0.24
Model control	12	21.75±2.56	107.83±9.21	4.92±0.24^**
XJEK low-dose	10	22.60±3.05	102.90±13.93	4.48±0.35^△△
XJEK medium-dose	10	23.45±3.40	108.00±15.89	4.50±0.31^△△
XJEK high-dose	10	24.30±3.29	105.20±14.73	4.34±0.40^△△
XJEK water layer	11	23.82±1.82	108.36±15.04	4.54±0.35^△
XJEK n-butanol layer	10	23.50±1.27	103.30±9.93	4.33±0.32^△△
Metoprolol	9	23.94±2.43	103.22±11.29	4.31±0.24^△△

Group	n	CSA (arbitrary unit)	CVF (%)	PVCA (%)	Hyp (μg/mg heart tissue)
Normal control	9	244.11±22.90	1.80±0.62	2.85±0.52	0.23±0.05
Model control	12	307.11±33.48^**	5.89±0.85^**	8.79±1.53^**	0.27±0.06^*
XJEK low-dose	10	292.96±20.06	5.11±0.71	7.65±0.95	0.24±0.05
XJEK medium-dose	10	290.18±26.23	5.26±0.78	7.56±0.97	0.23±0.03^△
XJEK high-dose	10	274.14±16.45^△△	3.47±0.75^△△	4.98±0.89^△△	0.21±0.03^△
XJEK water layer	11	229.14±32.38	5.28±0.86	7.82±1.00	0.25±0.04
XJEK n-butanol layer	10	262.04±20.69^△△	3.31±0.63^△△	5.21±0.65^△△	0.26±0.04
Metoprolol	9	249.19±20.16^△△	2.83±0.66^△△	4.41±0.83^△△	0.20±0.06^△

Group	n	SOD (kU/L)	MDA (μmol/L)
Normal control	9	104.42±8.31	5.26±0.62
Model control	12	94.10±5.26^**	6.30±0.51^**
XJEK low-dose	10	98.19±6.21	5.98±0.39
XJEK medium-dose	10	100.74±6.11^△△	5.66±0.46^△△
XJEK high-dose	10	101.51±4.97^△△	5.74±0.41^△△
XJEK water layer	11	96.02±6.55	5.97±0.35
XJEK n-butanol layer	10	95.39±5.23	6.05±0.43
Metoprolol	9	101.36±4.88^△△	5.55±0.43^△△

Effects of a compound Chinese medicine Xinji’erkang on isoproterenol-induced ventricular remodeling in mice

RichHTML

PDF (PC)

Like