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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (3): 330-336.doi: 10.3736/jcim20120313

• Original Experimental Research • Previous Articles     Next Articles

Effects of a compound Chinese medicine Xinji’erkang on isoproterenol-induced ventricular remodeling in mice

Gao Shan1,Wang Xing-hui1,Huang Ling-ling1,Yu Ting-ting1,Du Su-ming1,Guo Yan-wei1,Jia Yuan1,Wang Jian2()   

  1. 1. Department of Pharmacology, College of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China
    2. Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China
  • Received:2011-09-21 Accepted:2011-11-09 Online:2012-03-20 Published:2018-10-10

OBJECTIVE: To investigate the effects of Xinji’erkang (XJEK), a compound Chinese herbal medicine, on isoproterenol-induced ventricular remodeling in mice.
METHODS: Isoproterenol was given subcutaneously (1 mg/kg, twice per day for 7 d) to induce ventricular remodeling in mice. Mice were divided into normal control group, model group, XJEK low-, medium- and high-dose groups, XJEK water layer group, XJEK n-butanol layer group and metoprolol group. All drugs were given by intragastric administration. At the end of the 7th day, the hearts of the rats were weighted, and myocardial hypertrophy index was expressed as heart weight/body weight (HW/BW). The histological changes were observed by hemotoxylin-eosin and Van Gieson staining. Colorimetric method was used to determine the content of hydroxyproline in heart, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in serum.
RESULTS: Compared with the isoproterenol injection only, XJEK potently inhibited cardiomyocyte hypertrophy and the increase of hydroxyproline content in heart (P<0.01), improved cardiac pathology change, inhibited the decrease of SOD activity and the increase of MDA content in serum (P<0.01). XJEK water layer also inhibited the increase of cardiomyocyte hypertrophy (P<0.01) while XJEK n-butanol layer inhibited cardiomyocyte hypertrophy and fibrosis (P<0.01).
CONCLUSION: XJEK possesses protective effects against isoproterenol-induced ventricular remodeling in mice, which may be related to its actions in reducing the oxidative stress and improving the antioxidant activity of the body. XJEK water layer and XJEK n-butanol layer attenuated ventricular remodeling without significant oxidative stress state changing, which indicates that a non-antioxidative stress mechanism may exist.

Key words: compounds, traditional Chinese drugs, isoproterenol, ventricular remodeling, oxdative stress, superoxide dismutase, malonyldialdehyde, mice

Table 1

Effects of XJEK on heart weight index in mice with isoprenaline-induced ventricular remodeling (x±s)"

Group n BW (g) HW (mg) HW/BW (mg/g)
Normal control 9 20.94±2.13 82.33±9.62 3.93±0.24
Model control 12 21.75±2.56 107.83±9.21 4.92±0.24**
XJEK low-dose 10 22.60±3.05 102.90±13.93 4.48±0.35△△
XJEK medium-dose 10 23.45±3.40 108.00±15.89 4.50±0.31△△
XJEK high-dose 10 24.30±3.29 105.20±14.73 4.34±0.40△△
XJEK water layer 11 23.82±1.82 108.36±15.04 4.54±0.35
XJEK n-butanol layer 10 23.50±1.27 103.30±9.93 4.33±0.32△△
Metoprolol 9 23.94±2.43 103.22±11.29 4.31±0.24△△

Table 2

Effects of XJEK on CSA, CVF, PVCA and hydroxyproline content in heart tissue of mice with isoprenaline-induced ventricular remodeling (x±s)"

Group n CSA (arbitrary unit) CVF (%) PVCA (%) Hyp (μg/mg heart tissue)
Normal control 9 244.11±22.90 1.80±0.62 2.85±0.52 0.23±0.05
Model control 12 307.11±33.48** 5.89±0.85** 8.79±1.53** 0.27±0.06*
XJEK low-dose 10 292.96±20.06 5.11±0.71 7.65±0.95 0.24±0.05
XJEK medium-dose 10 290.18±26.23 5.26±0.78 7.56±0.97 0.23±0.03
XJEK high-dose 10 274.14±16.45△△ 3.47±0.75△△ 4.98±0.89△△ 0.21±0.03
XJEK water layer 11 229.14±32.38 5.28±0.86 7.82±1.00 0.25±0.04
XJEK n-butanol layer 10 262.04±20.69△△ 3.31±0.63△△ 5.21±0.65△△ 0.26±0.04
Metoprolol 9 249.19±20.16△△ 2.83±0.66△△ 4.41±0.83△△ 0.20±0.06

Figure 1

Effects of Xinji’erkang on CSA in mice with isoprenaline-induced ventricular remodeling tested by hematoxylin-eosin staining (Light microscopy, ×400) A: Myocardial interstitial fibrosis; B: Myocardial perivascular fibrosis. a: Normal control; b: Model control; c: XJEK low-dose; d: XJEK medium-dose; e: XJEK high-dose; f: XJEK water layer; g: XJEK n-butanol layer: h: Metoprolol. CSA: cross section area; XJEK: Xinji’erkang."

Figure 2

Effects of XJEK on CVF and PVCA in mice with isoprenaline-induced ventricular remodeling tested by Van Gieson staining (Light microscopy, ×400) A: Myocardial interstitial fibrosis; B: Myocardial perivascular fibrosis. a: Normal control; b: Model control; c: XJEK low-dose; d: XJEK medium-dose; e: XJEK high-dose; f: XJEK water layer; g: XJEK n-butanol layer: h: Metoprolol. XJEK: Xinji’erkang; CVF: collagen volume fraction; PVCA: perivascular circumferential collagen area."

Table 3

Effects of XJEK on serum SOD activity and MDA content in mice with isoprenaline-induced ventricular remodeling (x±s)"

Group n SOD (kU/L) MDA (μmol/L)
Normal control 9 104.42±8.31 5.26±0.62
Model control 12 94.10±5.26** 6.30±0.51**
XJEK low-dose 10 98.19±6.21 5.98±0.39
XJEK medium-dose 10 100.74±6.11△△ 5.66±0.46△△
XJEK high-dose 10 101.51±4.97△△ 5.74±0.41△△
XJEK water layer 11 96.02±6.55 5.97±0.35
XJEK n-butanol layer 10 95.39±5.23 6.05±0.43
Metoprolol 9 101.36±4.88△△ 5.55±0.43△△
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