Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (7): 784-792.doi: 10.3736/jcim20120709

• Original Experimental Research • Previous Articles     Next Articles

Effectiveness of flavonoid-rich leaf extract of Acalypha indica in reversing experimental myocardial ischemia: biochemical and histopathological evidence

C. Mouli Kalla1,Vijaya Tartte2(),Dattatreya Rao Sirpurkar3   

  1. 1. Department of Biotechnology, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India
    2. Department of Botany, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India
    3. Department of Shalya, Sri Venkateswara Ayurvedic College, Tirupati 517502, Andhra Pradesh, India
  • Received:2012-01-26 Accepted:2012-02-28 Online:2012-07-20 Published:2018-10-20
  • Contact: Vijaya Tartte

OBJECTIVE: In this study, we investigated the antimyocardial ischemic effects of flavonoid-rich methanolic leaf extract of Acalypha indica (AIE).
METHODS: An animal model of myocardial ischemic injury was induced by isoproterenol (ISO) in adult rats. Albino Wistar rats were pretreated with the AIE (100 and 200 mg/kg body weight orally) for 30 d followed by ISO (85 mg/kg subcutaneously) at an interval of 24 h for 2 d. At the end of the experimental period (12 h after the second dose of ISO injection), rats were sacrificed by anaesthetization with an intramuscular injection of ketamine hydrochloride (24 mg/kg). To ensure anti-ischemic potential of AIE, the plasma lipids such as total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C), free fatty acids (FFA), phospholipids (PL), myocardial lipids and hepatic lipids (TC, TG, FFA and PL) were estimated. Histopathology of heart tissue was also examined.
RESULTS: Administration of AIE maintained the levels of plasma lipids in all the treatment groups (100 and 200 mg/kg) when compared with the ISO-injected model rats. Histopathological examination of heart tissue of ISO-administered model rat showed myofiber loss, extensive subendocardial necrosis, infiltration of inflammatory cells, marked myocellular edema and vacuolar degeneration. However, pretreatment with AIE at 200 mg/kg showed predominantly normal myocardium structure with myofibers appeared and no inflammatory cell infiltration, edema and necrosis.
CONCLUSION: The biochemical and histological evidence from this study shows that AIE is protective against ISO-induced myocardial ischemia.

Key words: Acalypha indica, myocardial ischemia, plant extracts, flavonoids, antilipemic agents, rats


Group n TC (mg/L) TAG (mg/L) HDL-C (mg/L) LDL-C (mg/L) VLDL-C (mg/L)
Control 6 7.475±0.614 5.278±0.327 4.278±0.324 2.141±0.831 1.055±0.065
Model (isoproterenol 85 mg/kg) 6 10.758±0.919* 7.293±0.577* 3.078±0.226* 6.261±0.705* 1.458±0.115*
Isoproterenol plus AIE (100 g/kg) 6 9.256±0.646 5.958±0.323 4.516±0.305 3.548±0.481 1.191±0.064
Isoproterenol plus AIE (200 g/kg) 6 8.573±0.585 5.381±0.291 4.348±0.253 3.148±0.649 1.076±0.058
Group n FFA (mg/L) PL (mg/L) AIP Non-HDL-C (mg/L)
Control 6 5.018±0.357 8.035±0.622 –0.027±0.005 3.196±0.854
Model (isoproterenol 85 mg/kg) 6 6.881±0.639* 11.961±1.068* 0.001±0.004* 7.680±0.768*
Isoproterenol plus AIE (100 g/kg) 6 5.913±0.391 8.886±0.787 –0.024±0.002 4.740±0.507
Isoproterenol plus AIE (200 g/kg) 6 5.348±0.344 7.468±0.525 –0.022±0.000 4.220±0.626


Group n Myocardial lipids
Total cholesterol TAG FFA PL
Control 6 3.56±0.12 3.74±0.11 3.95±0.16 13.04±1.41
Model (isoproterenol 85 mg/kg) 6 4.94±0.10* 5.49±0.18* 5.02±0.20* 9.31±0.92*
Isoproterenol plus AIE (100 g/kg) 6 4.21±0.19 4.61±0.16 4.52±0.16 12.26±1.05
Isoproterenol plus AIE (200 g/kg) 6 3.82±0.11 4.36±0.12 4.11±0.18 11.83±1.06
Group n Hepatic lipids
Total cholesterol TAG FFA PL
Control 6 4.28±0.26 4.68±0.28 6.59±0.34 20.11±1.60
Model (isoproterenol 85 mg/kg) 6 5.81±0.23* 6.62±0.25* 11.02±1.01* 14.28±1.13*
Isoproterenol plus AIE (100 g/kg) 6 5.14±0.19 5.83±0.19 7.93±0.49 17.18±1.32
Isoproterenol plus AIE (200 g/kg) 6 4.91±0.17 5.21±0.27 7.47±0.33 16.49±1.21

Figure 1

Photomicrographs showing histopathology of heart tissue in rats by hematoxylin-eosin staining (Light microscopy, ×40) A: Normal struefure of myocardium of rats in the control group; B: Extensive myocardial necrosis and inflammatory cell infiltration in model group; C: Myocardium with the presence of sparse inflammatory cells and focal edema in AIE 100 mg/kg group; D: Myocardium in AIE 200 mg/kg group. Figures are representative of at least four independent experiments. AIE: Acalypha indica extract."

[1] Shimokawa H, Yasuda S . Myocardial ischemia: current concepts and future perspectives[J]. J Cardiol, 2008,52(2):67-78
doi: 10.1016/j.jjcc.2008.07.016 pmid: 18922380
[2] Abhilash PA, Nisha P, Prathapan A, Nampoothiri SV, Lijo Cherian O, Sunitha TK, Raghu KG . Cardioprotective effects of aqueous extract of Oxalis corniculata in experimental myocardial infarction[J]. Exp Toxicol Pathol, 2011,63(6):535-540
doi: 10.1016/j.etp.2010.04.004
[3] Smith GD, Shipley MJ, Marmot MG, Rose G . Plasma cholesterol concentration and mortality. The Whitehall Study[J]. JAMA, 1992,267(1):70-76
doi: 10.1001/jama.1992.03480010078028
[4] Grimm D, Elsner D, Schunkert H, Pfeifer M, Griese D, Bruckschlegel G, Muders F, Riegger GA, Kromer EP . Development of heart failure following isoproterenol administration in the rat: role of the renin-angiotensin system[J]. Cardiovas Res, 1998,37(1):91-100
doi: 10.1016/S0008-6363(97)00212-5
[5] Nair PS, Shyamala Devi CS . Efficacy of mangiferin on serum and heart tissue lipids in rats subjected to isoproterenol induced cardiotoxicity[J]. Toxicology, 2006,228(2-3):135-139
doi: 10.1016/j.tox.2006.08.030
[6] Bandyopadhyay D, Chattopadhyay A, Ghosh G, Datta AG . Oxidative stress-induced ischemic heart disease: protection by antioxidants[J]. Curr Med Chem, 2004,11(3):369-387
doi: 10.2174/0929867043456016 pmid: 14965238
[7] Young IS, Woodside JV . Antioxidants in health and disease[J]. J Clin Pathol, 2001,54(3):176-186
doi: 10.1136/jcp.54.3.176 pmid: 1731363
[8] Kirtikar KR ,Basu BD. Indian medicinal plant. Vol Ⅲ. 2nd ed[M]. Dehradun: International Book Distributors, 1999: 2262-2263
[9] Raj J ,Singh KP. Acalypha indica. CCRH Q Bull. 2000; 22(1-2): 1-6.
[10] Nahrstedt A, Hungeling M ,Petereit F. Flavonoids from Acalypha indica. Fitoterapia. 2006; 77(6): 484-486.
doi: 10.1016/j.fitote.2006.04.007
[11] Jia Z, Tang M, Wu J . The determination of flavonoids content in mulberry and scavenging effect on superoxide radicals[J]. Food Chem, 1999,64(4):555-559
doi: 10.1016/S0308-8146(98)00102-2
[12] Senthil S, Sridevi M, Pugalendi KV . Cardioprotective effect of oleanolic acid on isoproterenol-induced myocardial ischemia in rats[J]. Toxicol Pathol, 2007,35(3):418-423
doi: 10.1080/01926230701230312 pmid: 17455091
[13] Suri R, Abu Bakar H, Noor Rehan A, Rosnah O, Normah A . Preliminary studies on the analysis of fatty acids, essential oils and flavonoids in Acalypha indica L. J Trop Agric Food Sci. 2004; 32(2): 163-169.
[14] Zilversmit DB, Davis AK . Microdetermination of plasma phospholipids by trichloroacetic acid precipitation[J]. J Lab Clin Med, 1950,35(1):155-160
doi: 10.1038/jid.1950.128 pmid: 15400638
[15] Falholt K, Lund B, Falholt W . An easy colorimetric micromethod for routine determination of free fatty acids in plasma[J]. Clin Chim Acta, 1973,46(2):105-111
doi: 10.1016/0009-8981(73)90016-8 pmid: 4745354
[16] Friedewald WT, Levy RI, Fredrickson DS . Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge[J]. Clin Chem, 1972,18(6):449-502
[17] Folch J, Lees M, Sloane Stanley GH . A simple method for the isolation and purification of total lipides from animal tissues[J]. J Biol Chem, 1957,226(1):497-509
[18] Zlatkis A, Zak B, Boyle AJ . A new method for the direct determination of serum cholesterol[J]. J Lab Clin Med, 1953,41(3):486-492
doi: 10.1136/jcp.18.6.824 pmid: 13035283
[19] Foster LB, Dunn RT . Stable reagents for determination of serum triglycerides by a colorimetric Hantzsch condensation method[J]. Clin Chem, 1973,19(3):338-340
[20] Fiske CH ,Subbarow Y. The colorimetric determination of phosphorus[J]. J Biol Chem, 1925, 66: 375-400
doi: 10.2307/1536654 pmid: 16744823
[21] Rajadurai M , Stanely Mainzen Prince P. Preventive effect of naringin on lipids, lipoproteins and lipid metabolic enzymes in isoproterenol-induced myocardial infarction in Wistar rats[J]. J Biochem Mol Toxicol, 2006,20(4):191-197
doi: 10.1002/(ISSN)1099-0461
[22] Langin D . Control of fatty acid and glycerol release in adipose tissue lipolysis[J]. C R Biol, 2006,329(8):598-607
doi: 10.1016/j.crvi.2005.10.008 pmid: 16860278
[23] Sharma M, Kishore K, Gupta SK, Joshi S, Arya DS . Cardioprotective potential of Ocimum sanctum in isoproterenol induced myocardial infarction in rats[J]. Mol Cell Biochem, 2001,225(1-2):75-83
doi: 10.1023/A:1012220908636
[24] Raju K, Balaraman R, Hariprasad A, Vinoth Kumar M, Ali A . Cardioprotective effect of Momordica cymbalaria Fenzl in rats with isoproterenol-induced myocardial injury[J]. J Clin Diagn Res, 2008,2(1):699-705
[25] Zhao SP, Xu DY . Oxidized lipoprotein(a) increases the expression of platelet-derived growth factor-B in human umbilical vein endothelial cells[J]. Clin Chim Acta, 2000,296(1-2):121-133
doi: 10.1016/S0009-8981(00)00214-X
[26] Sheela Sasikumar C, Shyamala Devi CS . Protective effect of Abana, a poly-herbal formulation, on isoproterenol-induced myocardial infarction in rats[J]. Indian J Pharmacol, 2000,32(3):198-201
[27] Ravi K, Rajasekaran S, Subramanian S . Antihyperlipidemic effect of Eugenia jambolana seed kernel on streptozotocin-induced diabetes in rats[J]. Food Chem Toxicol, 2005,43(9):1433-1439
doi: 10.1016/j.fct.2005.04.004
[28] Ng TKW, Teh CB, Vidyadaran MK, Tee ES, Thong ML, Kandiah M, Ehalid AH . A critical evaluation of high density lipoprotein cholesterol as an index of coronary artery disease risk in Malaysians[J]. Mal J Nutr, 1997,3(1):61-70
[29] Zou Y, Lu Y, Wei D . Hypocholesterolemic effects of a flavonoid-rich extract of Hypericum perforatum L. in rats fed a cholesterol-rich diet[J]. J Agric Food Chem, 2005,53(7):2462-2466
doi: 10.1021/jf048469r
[30] Fan C, Yan J, Qian Y, Wo X, Gao L . Regulation of lipoprotein lipase expression by effect of hawthorn flavonoids on peroxisome proliferator response element pathway[J]. J Pharmacol Sci, 2006,100(1):51-58
doi: 10.1254/jphs.FP0050748
[31] Guo L, Hu WR, Lian JH, Ji W, Deng T, Qian M, Gong BQ . Anti-hyperlipidemic properties of CM108(a flavone derivative) in vitro and in vivo[J]. Eur J Pharmacol, 2006,551(1-3):80-86
doi: 10.1016/j.ejphar.2006.08.048 pmid: 17026988
[32] Vijaya Padma V, Shyamala Devi CS, Ramkumar KM . Effect of fish oil pretreatment on isoproterenol-induced changes in myocardial membrane phospholipids[J]. Nutrition, 2006,22(11-12):1171-1176
doi: 10.1016/j.nut.2006.08.010
[33] Reddy BV, Sivagama Sundari J, Balamurugan E, Menon VP . Antihyperlipidemic effect of bis-1,7-(2-hydroxyphenyl)-hepta-1,6-diene-3,5-dione, a curcumin analog, on nicotine and streptozotocin treated rats[J]. Mol Cell Biochem, 2010,335(1-2):249-254
doi: 10.1007/s11010-009-0274-3
[34] Dobiásǒová M, Frohlich J . The plasma parameter log(TG/HDL-C) as an atherogenic index: correlation with lipoprotein particle size and esterification rate in apoB-lipoprotein-depleted plasma(FERHDL)[J]. Clin Biochem, 2001,34(7):583-588
doi: 10.1016/S0009-9120(01)00263-6
[35] Shaw EB, Carter JB, McMaster KR, Daniel BW, Moffatt JE, Armstrong WR, Jones RO ,Spalding JM. Non-HDL cholesterol[J]. Clin Pathol News Med Commun, 2009, 11: 1-2
[36] Venter H, Genade S, Mouton R, Huisamen B, Harper IS, Lochner A . Myocardial membrane cholesterol: effects of ischaemia[J]. J Mol Cell Cardiol, 1991,23(11):1271-1286
doi: 10.1016/0022-2828(91)90084-Y pmid: 1803018
[37] Prince PS, Rajadurai M . Preventive effect of Aegle marmelos leaf extract on isoprenaline-induced myocardial infarction in rats: biochemical evidence[J]. J Pharm Pharmacol, 2005,57(10):1353-1357
doi: 10.1211/jpp.57.10.0015
[38] Paritha IA, Shyamala DCS . Effect of α-tocopherol on isoproterenol-induced changes in lipid and lipoprotein profile in rats[J]. Indian J Pharmacol, 1997,29(6):399-404
[39] Yotsumoto H, Yanagita T, Yamamoto K, Ogawa Y, Cha JY, Mori Y . Inhibitory effects of oren-gedoku-to and its components on cholesteryl ester synjournal in cultured human hepatocyte HepG2 cells: evidence from the cultured HepG2 cells and in vitro assay of ACAT[J]. Planta Med, 1997,63(2):141-145
doi: 10.1055/s-2006-957631
[40] Igarashi K, Ohmuma M . Effects of isorhamnetin, rhamnetin, and quercetin on the concentrations of cholesterol and lipoperoxide in the serum and liver and on the blood and liver antioxidative enzyme activities of rats[J]. Biosci Biotechnol Biochem, 1995,59(4):595-601
doi: 10.1271/bbb.59.595
[41] Bok SH, Lee SH, Park YB, Bae KH, Son KH, Jeong TS, Choi MS . Plasma and hepatic cholesterol and hepatic activities of 3-hydroxy-3-methyl-glutaryl-CoA reductase and acyl CoA: cholesterol transferase are lower in rats fed citrus peel extract or a mixture of citrus bioflavonoids[J]. J Nutr, 1999,129(6):1182-1185
doi: 10.1093/jn/129.6.1182
[42] Teli MR, James OF, Burt AD, Bennett MK, Day CP . The natural history of nonalcoholic fatty liver: a follow-up study[J]. Hepatology, 1995,22(6):1714-1719
doi: 10.1002/hep.1840220616 pmid: 7489979
[43] Feldstein AE, Werneburg NW, Canbay A, Guicciardi ME, Bronk SF, Rydzewski R, Burgart LJ, Gores GJ . Free fatty acids promote hepatic lipotoxicity by stimulating TNF-α expression via a lysosomal pathway[J]. Hepatology, 2004,40(1):185-194
doi: 10.1002/hep.v40:1
[44] Nassuato G, Iemmolo RM, Strazzabosco M, Lirussi F, Deana R, Francesconi MA, Muraca M, Passera D, Fragasso A, Orlando R, Csomós G , Okolicsanyi L . Effect of Silibinin on biliary lipid composition. Experimental and clinical study[J]. J Hepatol, 1991,12(3):290-295
doi: 10.1016/0168-8278(91)90829-Z pmid: 1940257
[45] Borradaile NM, Carroll KK, Kurowska EM . Regulation of HepG2 cell apolipoprotein B metabolism by the citrus flavanones hesperetin and naringenin[J]. Lipids, 1999,34(6):591-598
doi: 10.1007/s11745-999-0403-7
[46] Johannsen TH, Kamstrup PR, Andersen RV, Jensen GB, Sillesen H, Tybjaerg-Hansen A, Nordestgaard BG . Hepatic lipase, genetically elevated high-density lipoprotein, and risk of ischemic cardiovascular disease[J]. J Clin Endocrinol Metab, 2009,94(4):1264-1273
doi: 10.1210/jc.2008-1342
[1] Wan-jun Guo, Yi Wang, Yu Deng, Lin-yan Cheng, Xin Liu, Ruo-fan Xi, Sheng-jie Zhu, Xin-yi Feng, Liang Hua, Kan Ze, Jian-yong Zhu, Dong-jie Guo, Fu-lun Li. Therapeutic effects of the extract of Sancao Formula, a Chinese herbal compound, on imiquimod-induced psoriasis via cysteine-rich protein 61. Journal of Integrative Medicine, 2022, 20(4): 376-384.
[2] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[3] Eric Wei Chiang Chan, Siu Kuin Wong, Joseph Tangah, Tomomi Inoue, Hung Tuck Chan. Phenolic constituents and anticancer properties of Morus alba (white mulberry) leaves. Journal of Integrative Medicine, 2020, 18(3): 189-195.
[4] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[5] Stefania Lamponi, Anna Maria Aloisi, Claudia Bonechi, Marco Consumi, Alessandro Donati, Gemma Leone, Claudio Rossi, Gabriella Tamasi, Luana Ghiandai, Ersilia Ferrini, Paolo Fiorenzani, Ilaria Ceccarelli, Agnese Magnani. Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements. Journal of Integrative Medicine, 2019, 17(3): 213-220.
[6] Rohitash Jamwal. Bioavailable curcumin formulations: A review of pharmacokinetic studies in healthy volunteers. Journal of Integrative Medicine, 2018, 16(6): 367-374.
[7] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Thongchai Koobkokkruad, Chutichot Pattamadilok, Nontima Vardhanabhuti . Anti-adipogenic effect of flavonoids from Chromolaena odorata leaves in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2018, 16(6): 427-434.
[8] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[9] Shohini Chakraborty, Nashra Afaq, Neelam Singh, Sukanta Majumdar. Antimicrobial activity of Cannabis sativaThuja orientalis and Psidium guajava leaf extracts against methicillin-resistant Staphylococcus aureus. Journal of Integrative Medicine, 2018, 16(5): 350-357.
[10] Bruno José Martins Da Silva, Amanda Anastácia Pinto Hage, Edilene Oliveira Silva, Ana Paula Drummond Rodrigues. Medicinal plants from the Brazilian Amazonian region and their antileishmanial activity: A review. Journal of Integrative Medicine, 2018, 16(4): 211-222.
[11] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[12] Pathom Somwong, Rutt Suttisri. Cytotoxic activity of the chemical constituents of Clerodendrum indicum and Clerodendrum villosum roots. Journal of Integrative Medicine, 2018, 16(1): 57-61.
[13] Morad Hebi, Mohamed Eddouks. Hypolipidemic activity of Tamarix articulata Vahl. in diabetic rats. Journal of Integrative Medicine, 2017, 15(6): 476-482.
[14] Nisha Panth, Keshav Raj Paudel, Rajendra Karki. Phytochemical profile and biological activity of Juglans regia. Journal of Integrative Medicine, 2016, 14(5): 359-373.
[15] Musa Toyin Yakubu, Abdulquadri Funmbi Olutoye. Aphrodisiac activity of aqueous extract of Anthonotha macrophylla P. Beauv. leaves in female Wistar rats. Journal of Integrative Medicine, 2016, 14(5): 400-408.
Full text



[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9] Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278