Curcumin alters motor coordination but not total number of Purkinje cells in the cerebellum of adolescent male Wistar rats

doi:10.3736/jintegrmed2013006

Abstract

Objective

The present study aimed at investigating the effects of curcumin on the motor coordination and the estimate of the total number of cerebellar Purkinje cells of adolescent Wistar rats exposed to ethanol.

Methods

The total of 21 male Wistar rats aged 37 d old were divided into three groups, namely ethanol, ethanol-curcumin, and control groups. The ethanol group received 1.5 g/kg ethanol injected intraperitoneally and water given per oral; the ethanol-curcumin group received 1.5 g/kg ethanol injected intraperitoneally and curcumin extract given per oral; the control group received saline injection and oral water. The treatment was carried out daily for one month, after which the motor coordination performance of the rats was examined using revolving drum apparatus at test days 1, 8, and 15. The rats were finally sacrificed and the cerebellum of the rats was further processed for stereological analysis. The estimate of the total number of Purkinje cells was calculated using physical fractionator method.

Results

The ethanol-curcumin group performed better than both ethanol and control groups in the motor coordination ability at day 8 of testing (P<0.01). No Purkinje cell loss was observed as a result of one month intraperitoneal injection of ethanol.

Conclusion

Curcumin may exert beneficial effects on the motor coordination of adolescent rats exposed to ethanol via undetermined hormetic mechanisms.

Key words: Curcumin, Purkinje cells, Antioxidants, Rats, Wistar

Ginus Partadiredja, Taufik Nur Yahya, Christiana Tri Nuryana, Rina Susilowati. Curcumin alters motor coordination but not total number of Purkinje cells in the cerebellum of adolescent male Wistar rats. Journal of Chinese Integrative Medicine, 2013, 11(1): 32-38.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jintegrmed2013006

http://www.jcimjournal.com/EN/Y2013/V11/I1/32

[1]	Maggs JL , Schulenberg JE. Trajectories of alcohol use during the transition to adulthood. Alcohol Res Health. 2004/2005 ; 28(4):195-201.
[2]	Ahsltrm SK , sterberg EL. International perspectives on adolescent and young adult drinking. Alcohol Res Health. 2004 /2005; 28(4):258-268.
[3]	Shankar K, Ronis MJ, Badger TM . Effects of pregnancy and nutritional status on alcohol metabolism[J]. Alcohol Res Health, 2007,30(1):55-59
[4]	Butterworth RF . Hepatic encephalopathy — a serious complication of alcoholic liver disease[J]. Alcohol Res Health, 2003,27(2):143-145
[5]	Lukoyanov NV , Brand?o F, Cadete-Leite A, Madeira MD, Paula-Barbosa MM.Synaptic reorganization in the hippocampal formation of alcohol-fed rats may compensate for functional deficits related to neuronal loss[J]. Alcohol, 2000,20(2):139-148 doi: 10.1016/S0741-8329(99)00069-5
[6]	Miki T, Harris SJ, Wilce P, Takeuchi Y, Bedi KS . Neurons in the hilus region of the rat hippocampus are depleted in number by exposure to alcohol during early postnatal life[J]. Hippocampus, 2000,10(3):284-295 doi: 10.1002/(ISSN)1098-1063
[7]	Miki T, Harris SJ, Wilce PA, Takeuchi Y, Bedi KS . Effects of age and alcohol exposure during early life on pyramidal cell numbers in the CA1-CA3 region of the rat hippocampus[J]. Hippocampus, 2004,14(1):124-134 doi: 10.1002/(ISSN)1098-1063
[8]	Tran TD, Kelly SJ . Critical periods for ethanol-induced cell loss in the hippocampal formation[J]. Neurotoxicol Teratol, 2003,25(5):519-528 doi: 10.1016/S0892-0362(03)00074-6
[9]	Miki T, Harris S, Wilce P, Takeuchi Y, Bedi KS . The effect of the timing of ethanol exposure during early postnatal life on total number of Purkinje cells in rat cerebellum[J]. J Anat, 1999,194(Pt 3):423-431 doi: 10.1046/j.1469-7580.1999.19430423.x
[10]	Maier SE, West JR . Regional differences in cell loss associated with binge-like alcohol exposure during the first two trimesters equivalent in the rat[J]. Alcohol, 2001,23(1):49-57 doi: 10.1016/S0741-8329(00)00133-6
[11]	Deitrich R, Zimatkin S, Pronko S . Oxidation of ethanol in the brain and its consequences[J]. Alcohol Res Health, 2006,29(4):266-273
[12]	Zakhari S . Overview: how is alcohol metabolized by the body? Alcohol Res Health. 2006; 29(4):245-254.
[13]	Bala K, Tripathy BC, Sharma D . Neuroprotective and anti-ageing effects of curcumin in aged rat brain regions[J]. Biogerontology, 2006,7(2):81-89 doi: 10.1007/s10522-006-6495-x
[14]	Jana NR, Dikshit P, Goswami A, Nukina N . Inhibition of proteasomal function by curcumin induces apoptosis through mitochondrial pathway[J]. J Biol Chem, 2004,279(12):11680-11685 doi: 10.1074/jbc.M310369200
[15]	Lim GP, Chu T, Yang F, Beech W, Frautschy SA, Cole GM . The curry spice curcumin reduces oxidative damage and amyloid pathology in an Alzheimer transgenic mouse[J]. J Neurosci, 2001,21(21):8370-8377 doi: 10.1523/JNEUROSCI.21-21-08370.2001
[16]	Atamna H, Boyle K . Amyloid-β peptide binds with heme to form a peroxidase: relationship to the cytopathologies of Alzheimer's disease[J]. Proc Natl Acad Sci U S A, 2006,103(9):3381-3386 doi: 10.1073/pnas.0600134103
[17]	Kumar P, Padi SS, Naidu PS, Kumar A . Possible neuroprotective mechanisms of curcumin in attenuating 3-nitropropionic acid-induced neurotoxicity[J]. Methods Find Exp Clin Pharmacol, 2007,29(1):19-25 doi: 10.1358/mf.2007.29.1.1063492
[18]	Kumar A, Naidu PS, Seghal N, Padi SS . Effect of curcumin on intracerebroventricular colchicine-induced cognitive impairment and oxidative stress in rats[J]. J Med Food, 2007,10(3):486-494 doi: 10.1089/jmf.2006.076
[19]	Rajeswari A . Curcumin protects mouse brain from oxidative stress caused by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine[J]. Eur Rev Med Pharmacol Sci, 2006,10(4):157-161
[20]	Spear L . Modeling adolescent development and alcohol use in animals[J]. Alcohol Res Health, 2000,24(2):115-123
[21]	Hiller-Sturmh?fel S , Swartzwelder HS. Alcohol’s effects on the adolescent brain. What can be learnd from animal models. Alcohol Res Health. 2004/2005; 28(4):213-221.
[22]	Jana K, Jana N, De DK, Guha SK . Ethanol induces mouse spermatogenic cell apoptosis in vivo through over-expression of Fas/Fas-L, p53, and caspase-3 along with cytochrome c translocation and glutathione depletion[J]. Mol Reprod Dev, 2010,77(9):820-833 doi: 10.1002/mrd.21227
[23]	Lundqvist C, Alling C, Knoth R, Volk B . Intermittent ethanol exposure of adult rats: hippocampal cell loss after one month of treatment[J]. Alcohol Alcohol, 1995,30(6):737-748
[24]	Smart JL, Bedi KS . Early life undernutrition in rats. 3. Motor performance in adulthood[J]. Br J Nutr, 1982,47(3):439-444 doi: 10.1079/BJN19820055
[25]	Partadiredja G, Bedi KS . Mice undernourished before, but not after, weaning perform better in motor coordination and spatial learning tasks than well-fed controls[J]. Nutr Neurosci, 2011,14(4):129-137 doi: 10.1179/147683011X13009738172486
[26]	Gundersen HJ . Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson[J]. J Microsc, 1986,143(Pt 1):3-45 doi: 10.1111/jmi.1986.143.issue-1
[27]	Meydani M, Hasan ST . Dietary polyphenols and obesity[J]. Nutrients, 2010,2(7):737-751 doi: 10.3390/nu2070737
[28]	Shehzad A, Ha T, Subhan F, Lee YS . New mechanisms and the anti-inflammatory role of curcumin in obesity and obesity-related metabolic diseases[J]. Eur J Nutr, 2011,50(3):151-161 doi: 10.1007/s00394-011-0188-1
[29]	Sood PK, Nahar U, Nehru B . Curcumin attenuates aluminum-induced oxidative stress and mitochondrial dysfunction in rat brain[J]. Neurotox Res, 2011,20(4):351-361 doi: 10.1007/s12640-011-9249-8
[30]	Shukla PK, Khanna VK, Khan MY, Srimal RC . Protective effect of curcumin against lead neurotoxicity in rat[J]. Hum Exp Toxicol, 2003,22(12):653-658 doi: 10.1191/0960327103ht411oa
[31]	Sharma V, Nehru B, Munshi A, Jyothy A . Antioxidant potential of curcumin against oxidative insult induced by pentylenetetrazol in epileptic rats[J]. Methods Find Exp Clin Pharmacol, 2010,32(4):227-232 doi: 10.1358/mf.2010.32.4.1452090
[32]	Kumar TP, Antony S, Gireesh G, George N, Paulose CS . Curcumin modulates dopaminergic receptor, CREB and phospholipase C gene expression in the cerebral cortex and cerebellum of streptozotocin induced diabetic rats[J]. J Biomed Sci, 2010,17:43 doi: 10.1186/1423-0127-17-43
[33]	Peeyush KT, Gireesh G, Jobin M, Paulose CS . Neuroprotective role of curcumin in the cerebellum of streptozotocin-induced diabetic rats[J]. Life Sci, 2009,85(19-20):704-710 doi: 10.1016/j.lfs.2009.09.012
[34]	Mattson MP, Son TG, Camandola S . Viewpoint: mechanisms of action and therapeutic potential of neurohormetic phytochemicals[J]. Dose Response, 2007,5(3):174-186
[35]	Rattan SI . Principles and practice of hormetic treatment of aging and age-related diseases[J]. Hum Exp Toxicol, 2008,27(2):151-154 doi: 10.1177/0960327107083409
[36]	Rattan SI . Aging, anti-aging, and hormesis[J]. Mech Ageing Dev, 2004,125(4):285-289 doi: 10.1016/j.mad.2004.01.006
[37]	Spear LP, Varlinskaya EI . Low dose effects in psychopharmacology: ontogenetic considerations[J]. Nonlinearity Biol Toxicol Med, 2005,3(1):97-111
[38]	Calabrese EJ, Baldwin LA . Ethanol and hormesis[J]. Crit Rev Toxicol, 2003,33(3-4):407-424 doi: 10.1080/713611043
[39]	Pauli J, Wilce P, Bedi KS . Acute exposure to alcohol during early postnatal life causes a deficit in the total number of cerebellar Purkinje cells in the rat[J]. J Comp Neurol, 1995,360(3):506-512 doi: 10.1002/(ISSN)1096-9861
[40]	Goodlett CR, Lundahl KR . Temporal determinants of neonatal alcohol-induced cerebellar damage and motor performance deficits[J]. Pharmacol Biochem Behav, 1996,55(4):531-540 doi: 10.1016/S0091-3057(96)00248-1
[41]	Goodlett CR, Eilers AT . Alcohol-induced Purkinje cell loss with a single binge exposure in neonatal rats: a stereological study of temporal windows of vulnerability[J]. Alcohol Clin Exp Res, 1997,21(4):738-744
[42]	Light KE, Belcher SM, Pierce DR . Time course and manner of Purkinje neuron death following a single ethanol exposure on postnatal day 4 in the developing rat[J]. Neuroscience, 2002,114(2):327-337 doi: 10.1016/S0306-4522(02)00344-5
[43]	Grisel JJ, Chen WJ . Antioxidant pretreatment does not ameliorate alcohol-induced Purkinje cell loss in the developing rat cerebellum[J]. Alcohol Clin Exp Res, 2005,29(7):1223-1229 doi: 10.1097/01.ALC.0000171932.13148.CF
[44]	Lee Y, Rowe J, Eskue K, West JR, Maier SE . Alcohol exposure on postnatal day 5 induces Purkinje cell loss and evidence of Purkinje cell degradation in lobule I of rat cerebellum[J]. Alcohol, 2008,42(4):295-302 doi: 10.1016/j.alcohol.2008.01.010
[45]	Idrus NM , McGough NN, Riley EP, Thomas JD.Administration of memantine during ethanol withdrawal in neonatal rats: effects on long-term ethanol-induced motor incoordination and cerebellar Purkinje cell loss[J]. Alcohol Clin Exp Res, 2011,35(2):355-364 doi: 10.1111/acer.2011.35.issue-2
[46]	Pierce DR, Williams DK, Light KE . Purkinje cell vulnerability to developmental ethanol exposure in the rat cerebellum[J]. Alcohol Clin Exp Res, 1999,23(10):1650-1659 doi: 10.1111/acer.1999.23.issue-10
[47]	Pentney RJ, Dlugos CA . Cerebellar Purkinje neurons with altered terminal dendritic segments are present in all lobules of the cerebellar vermis of ageing, ethanol-treated F344 rats[J]. Alcohol Alcohol, 2000,35(1):35-43 doi: 10.1093/alcalc/35.1.35
[48]	Dlugos CA, Pentney RJ . Morphometric evidence that the total number of synapses on Purkinje neurons of old F344 rats is reduced after long-term ethanol treatment and restored to control levels after recovery[J]. Alcohol Alcohol, 1997,32(2):161-172 doi: 10.1093/oxfordjournals.alcalc.a008250
[49]	Dlugos CA . Ethanol-related smooth endoplasmic reticulum dilation in purkinje dendrites of aging rats[J]. Alcohol Clin Exp Res, 2006,30(5):883-891 doi: 10.1111/acer.2006.30.issue-5
[50]	Dlugos CA . Ethanol-related increases in degenerating bodies in the Purkinje neuron dendrites of aging rats[J]. Brain Res, 2008,1221:98-107 doi: 10.1016/j.brainres.2008.05.015
[51]	White AM, Truesdale MC, Bae JG, Ahmad S, Wilson WA, Best PJ, Swartzwelder HS . Differential effects of ethanol on motor coordination in adolescent and adult rats[J]. Pharmacol Biochem Behav, 2002,73(3):673-677 doi: 10.1016/S0091-3057(02)00860-2

Related Articles 15

[1]	Talat Albukhari, Bassem Refaat, El-Sayed Bakr, Sameh Baz, Bodour Rajab, Hossam Gadalla, Mohamed El-Boshy. Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats. Journal of Integrative Medicine, 2022, 20(4): 348-354.
[2]	Keat Lam Ho, Phaik Har Yong, Chee Woon Wang, Umah Rani Kuppusamy, Chek Tung Ngo, Festo Massawe, Zhi Xiang Ng. Peperomia pellucida (L.) Kunth and eye diseases: A review on phytochemistry, pharmacology and toxicology. Journal of Integrative Medicine, 2022, 20(4): 292-304.
[3]	Lu Yin, Yun An, Xiao Chen, Hui-xin Yan, Tao Zhang, Xin-gang Lu, Jun-tao Yan. Local vibration therapy promotes the recovery of nerve function in rats with sciatic nerve injury. Journal of Integrative Medicine, 2022, 20(3): 265-273.
[4]	Zeinab Vafaeipour, Bibi Marjan Razavi, Hossein Hosseinzadeh. Effects of turmeric (Curcuma longa) and its constituent (curcumin) on the metabolic syndrome: An updated review. Journal of Integrative Medicine, 2022, 20(3): 193-203.
[5]	Jia-yi Zhao, Jin Pu, Jian Fan, Xin-yu Feng, Jian-wen Xu, Rong Zhang, Yan Shang. Tanshinone IIA prevents acute lung injury by regulating macrophage polarization. Journal of Integrative Medicine, 2022, 20(3): 274-280.
[6]	Marisa Casal. Improving the health and treatment success rates of in vitro fertilization patients with traditional chinese medicine: need for more robust evidence and innovative approaches. Journal of Integrative Medicine, 2022, 20(3): 187-192.
[7]	Ning Guo, Fei Wu, Mei Wu, Yuan Wang, Qing Lang, Xiao Lin, Yi Feng. Progress in the design and quality control of placeboes for clinical trials of traditional Chinese medicine . Journal of Integrative Medicine, 2022, 20(3): 204-212.
[8]	Yan-jun Lin, Kun-li Jiao, Bo Liu, Lu Fang, Shu Meng. Antiplatelet and myocardial protective effect of shexiang tongxin dropping pill in patients undergoing percutaneous coronary intervention: A randomized controlled trial. Journal of Integrative Medicine, 2022, 20(2): 126-134.
[9]	Peter Bablis, Henry Pollard, Anthony L. Rosner. Stress reduction via neuro-emotional technique to achieve the simultaneous resolution of chronic low back pain with multiple inflammatory and biobehavioural indicators: A randomized, double-blinded, placebo-controlled trial. Journal of Integrative Medicine, 2022, 20(2): 135-144.
[10]	Jose Antonio Castilla-Jimena, Isabel Ruiz-Pérez, Jesús Henares-Montiel. Impact of socioeconomic and health-related factors on consumption of homeopathic and natural remedies in Spain in 2006, 2011 and 2017. Journal of Integrative Medicine, 2022, 20(1): 52-56.
[11]	Jun-peng Yao, Li-ping Chen, Xian-jun Xiao, Ting-hui Hou, Si-yuan Zhou, Ming-min Xu, Kai Wang, Yu-jun Hou, Lin Zhang, Ying Li. Effectiveness and safety of acupuncture for treating functional constipation: An overview of systematic reviews. Journal of Integrative Medicine, 2022, 20(1): 13-25.
[12]	Ofer Baranovitch, Meirav Wolff-Bar, Meora Feinmesser, Chen Sade-Zaltz, Ilan Tsarfaty, Victoria Neiman. Searching for the emotional roots of breast cancer: A cross-disciplinary analysis integrating psychology, Chinese medicine, and oncology biomarkers. Journal of Integrative Medicine, 2022, 20(1): 57-64.
[13]	Deng-chao Wang, Miao Yu, Wen-xian Xie, Li-yan Huang, Jian Wei, Yue-hua Lei. Meta-analysis on the effect of combining Lianhua Qingwen with Western medicine to treat coronavirus disease 2019. Journal of Integrative Medicine, 2022, 20(1): 26-33.
[14]	Miriam Bazzicalupo, Laura Cornara, Bruno Burlando, Alberta Cascini, Marcella Denaro, Antonella Smeriglio, Domenico Trombetta. Carpobrotus edulis (L.) N.E.Br. extract as a skin preserving agent: from traditional medicine to scientific validation. Journal of Integrative Medicine, 2021, 19(6): 526-536.
[15]	Meng-ya Shan, Ying Dai, Xiao-dan Ren, Jing Zheng, Ke-bin Zhang, Bin Chen, Jun Yan, Zi-hui Xu. Berberine mitigates nonalcoholic hepatic steatosis by downregulating SIRT1-FoxO1-SREBP2 pathway for cholesterol synthesis. Journal of Integrative Medicine, 2021, 19(6): 545-554.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2]	Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3]	Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4]	Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5]	Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6]	Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7]	Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8]	Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9]	Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10]	Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278