Search JIM Advanced Search

Journal of Integrative Medicine ›› 2025, Vol. 23 ›› Issue (2): 195-208.doi: 10.1016/j.joim.2025.02.003

• Original Experimental Research • Previous Articles    

Aloin blocks the malignant behavior of lung squamous cell carcinoma cells and M2 macrophage polarization by modulating the NR3C2/MT1M axis

Ying-na Chen a, Jie-ya Lu b, Cheng-feng Gao c, Zhi-ruo Fang a, Yan Zhou d   

  1. a.School of Pharmacy, Changzhou University, Changzhou 213164, Jiangsu Province, China
    b.Department of Nephrology, Yixing Hospital of Traditional Chinese Medicine, Wuxi 214200, Jiangsu Province, China
    c.Faculty of Chinese Medicine, Macau University of Science and Technology, Macau 999078, China
    d.Department of Digestive Diseases, Changzhou Traditional Chinese Medicine Hospital, Changzhou 213003, Jiangsu Province, China
  • Received:2024-05-27 Accepted:2025-11-20 Online:2025-04-09 Published:2025-04-09
  • Contact: Ying-na Chen; Jie-ya Lu E-mail:chenyingna@cczu.edu.cn; ljy7011645@sina.com

Objective
Aloin, the main active component in Aloe vera (L.) Burm. f., has shown promising anti-tumor effects. This study investigated the impact of aloin in lung squamous cell carcinoma (LUSC) and explored its functional mechanism.
Methods
We analyzed the viability, migration, invasion, proliferation, and apoptosis of two LUSC cell lines after treatment with aloin. Target molecules of aloin and downstream target transcripts of nuclear receptor subfamily 3 group C member 2 (NR3C2) were predicted by bioinformatics. The biological functions of NR3C2 and metallothionein 1 M (MT1M) in the malignant properties of LUSC cells were determined. A co-culture system of LUSC cells with monocyte-derived macrophages was constructed. Mouse xenograft tumor models were generated to analyze the functions of aloin and NR3C2 in the tumorigenic activity of LUSC cells and macrophage polarization in vivo.
Results
Aloin suppressed malignant properties of LUSC cells in vitro. However, these effects were negated by the silencing of NR3C2. NR3C2 was found to activate MT1M transcription by binding to its promoter. Additional upregulation of MT1M suppressed the malignant behavior of LUSC cells augmented by NR3C2 silencing. Analysis of the M1 and M2 markers/cytokines in the macrophages or the culture supernatant revealed that aloin treatment or MT1M overexpression in LUSC cells enhanced M1 polarization while suppressing M2 polarization of macrophages, whereas NR3C2 silencing led to reverse trends. Consistent findings were reproduced in vivo.
Conclusion
This study demonstrated that aloin activates the NR3C2/MT1M axis to suppress the malignant behavior of LUSC cells and M2 macrophage polarization.

Key words: Aloin, NR3C2, MT1M, Macrophages, Lung squamous cell carcinoma

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!