Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (4): 355-360.doi: 10.3736/jcim20080406

• Original Clinical Research • Previous Articles     Next Articles

Investigation of gene expression profiles in patients with blood stasis syndrome

Xiao-juan Ma, Hui-jun Yin, (), Ke-ji Chen   

  1. Center of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
  • Received:2007-11-26 Online:2008-04-20 Published:2008-04-15
  • Contact: YIN Hui-jun E-mail:huijunyin@yahoo.com.cn

Objective: To investigate the differential gene expression profiles in patients with blood stasis syndrome by oligonucleotide microarray technique.
Methods: Sixteen patients with blood stasis syndrome were divided into patients with coronary heart disease (CAD) (n=8) and non-CAD patients (n=8) by using coronary angiography. The sex- and age-matched eight healthy persons were enrolled as control group. Venous bloods were collected for extracting RNA. Test-3 chip was first employed to examine the quality of samples. Then the samples were hybridized with Affymetrix U133 Plus 2.0 array to compare the gene expression profiles among the three groups. Gene-array scanner and gene chip operating software were applied to screen hybridization signals and analyze gene expression respectively. Based on the comparison of the three groups of samples, the differential genes related with blood stasis syndrome were analyzed by Gene Ontology (GO) and pathway, and confirmed by real-time reverse transcription polymerase chain reaction (RT-PCR).
Results: Forty-eight differential genes were found being associated with blood stasis syndrome, including 26 up-regulated genes and 22 down-regulated genes. Five of the forty-eight genes (10.4%) were related to inflammatory reaction and immune response through the GO analysis. In the pathway analysis, five of ten significant pathways were referred to inflammation and immune response. The results of real-time RT-PCR proved the accuracy of the gene chip.
Conclusion: Inflammatory- and immune-related genes have a remarkable predominance in blood stasis syndrome gene expression profiles, which may explain the function of inflammation and immune response in the occurrence and development of blood stasis syndrome.

CLC Number: 

  • R241.29

Table 1

Clinical characteristics of three groups"

Items CAD patients with blood
stasis syndrome (n=8)
Non-CAD patients with blood
stasis syndrome (n=8)
Healthy control
subjects (n=8)
Age*(years) 53 (48-69) 60 (43-71) 57 (37-63)
Sex (male/female) 7/1 7/1 7/1
BMI ($\bar{x}$±s, kg/m2) 25.29±3.31 24.48±4.01 24.64±1.70
Smoking (cases) 5 4 2
Drinking (cases) 2 3 3
Family history of CAD (cases) 6 3 3
Accumulated score of blood stasis syndrome ($\bar{x}$±s) 26.63±5.24 22.88±6.58 -
Hypertension (cases) 5 6 -
Dyslipidemia (cases) 3 4 -
Diabetes (case) 1 1 -

Figure 1

Electrophoresis results of rRNA in CAD patients with blood stasis syndrome Samples one to five are the random samples picked from RNA pool in CAD patients with blood stasis syndrome. Ratio of electrophoresis band brightness between 28 S rRNA and 18 S rRNA is close to 2︰1."

Figure 2

Hybridization result of gene chip test3 with probe in non-CAD patients with blood stasis syndrome"

Table 2

Differential genes of blood stasis syndrome related with immune response and inflammation"

Gene ID Gene Title Fold change* 1 Fold change* 2
M16276 Major histocompatibility complex, class II 2.50 2.19
AF043337 Interleukin-8 2.46 2.52
AW007751 T cell receptor alpha locus –2.08 –2.67
NM_002261 Killer cell lectin-like receptor subfamily C –2.50 –2.60
W68403 Integrin, beta 2 –2.55 –2.29

Figure 3

Comparison between gene chip results and real-time RT-PCR identification The fold changes of 6 genes are not exactly identical, but the changing tendency are consistent between gene chip results and real-time RT-PCR identification."

[1] Chen KJ, Shi ZX. Practical workbook of blood-stasis syndrome[M]. Beijing: People's Medical Publishing House, 1999: 5-8
陈可冀, 史载祥 . 实用血瘀证学[M]. 北京: 人民卫生出版社, 1999: 5-8
[2] Xu H, Lu XY, Chen KJ , et al. Study on correlation of blood-stasis syndrome and its accompanied syndromes with pathological changes showed in coronary angiography and restenosis after percutaneous coronary intervention[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2007,27(1):8-13
doi: 10.3321/j.issn:1003-5370.2007.01.002
徐浩, 鹿小燕, 陈可冀 , 等. 血瘀证及其兼证与冠脉造影所示病变及介入治疗后再狭窄的相关性研究[J]. 中国中西医结合杂志, 2007,27(1):8-13
doi: 10.3321/j.issn:1003-5370.2007.01.002
[3] Zhong S, Storch KF, Lipan O , et al. GoSurfer: a graphical interactive tool for comparative analysis of large gene sets in Gene Ontology space[J]. Appl Bioinformatics, 2004,3(4):261-264
doi: 10.2165/00822942-200403040-00009
[4] Shi YH, Zhu SW, Mao XZ , et al. Transcriptome profiling, molecular biological and physiological studies reveal a major role for ethylene in cotton fiber cell elongation[J]. Plant Cell, 2006,18(3):651-664
doi: 10.1105/tpc.105.040303
[5] Mao X, Cai T, Olyarchuk JG , et al. Automated genome annotation and pathway identification using the KEGG Orthology(KO) as a controlled vocabulary[J]. Bioinformatics, 2005,21(15):3787-3793
doi: 10.1093/bioinformatics/bti430
[6] Mlecnik B, Scheideler M, Hackl H , et al. Pathway Explorer: web service for visualizing high-throughput expression data on biological pathways[J]. Nucleic Acids Res, 2005,33(Web Server issue):633-637
doi: 10.1093/nar/gki391
[7] Ma M, Zhang GJ . Research progress of blood stasis syndrome objectivity[J]. Shandong Zhong Yi Yao Da Xue Xue Bao, 2002,2(26):155-158
马民, 张桂娟 . 血瘀证客观化研究进展[J]. 山东中医药大学学报, 2002,2(26):155-158
[8] Ma XJ, Yin HJ, Chen KJ . Research progress of correlation between blood-stasis syndrome and inflammation[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2007,27(7):669-672
马晓娟, 殷惠军, 陈可冀 . 血瘀证与炎症相关性的研究进展[J]. 中国中西医结合杂志, 2007,27(7):669-672
[9] Ferroni P, Martini F, Cardarello CM , et al. Enhanced interleukin-1beta in hypercholesterolemia: effects of simvastatin and low-dose aspirin[J]. Circulation, 2003,108(14):1673-1675
doi: 10.1161/01.CIR.0000094732.02060.27
[10] Tang YH, Zhang SP, Liang Y , et al. Effects of Panax notoginseng saponins on mRNA expressions of interleukin-1β, its correlative factors and cysteinyl-aspartate specific protease after cerebral ischemia-reperfusion in rats[J]. Zhong Xi Yi Jie He Xue Bao, 2007,5(3):328-332
唐映红, 张淑萍, 梁燕 , 等. 三七总皂苷对大鼠脑缺血再灌注后白细胞介素1β和其相关因子及半胱氨酸天冬氨酸特异性蛋白酶表达的影响[J]. 中西医结合学报, 2007,5(3):328-332
[11] Burger PC, Wagner DD . Platelet P-selectin facilitates atherosclerotic lesion development[J]. Blood, 2003,101(7):2661-2666
doi: 10.1182/blood-2002-07-2209
[12] Nannizzi-Alaimo L, Alves VL, Phillips DR . Inhibitory effects of glycoprotein IIb/IIIa antagonists and aspirin on the release of soluble CD40 ligand during platelet stimulation[J]. Circulation, 2003,107(8):1123-1128
doi: 10.1161/01.CIR.0000053559.46158.AD
[13] Jiang H, Du M, Xi Y , et al. Genetic polymorphism of FcγRⅡA-genes in patients with coronary heart disease and dilated cardiomyopathy[J]. Guo Ji Yi Chuan Xue Za Zhi, 2006,29(4):246-248
doi: 10.3760/cma.j.issn.1673-4386.2006.04.002
姜红, 杜明, 西雁 , 等. Fcγ受体ⅡA基因多态性与冠心病和扩张型心肌病的关系[J]. 国际遗传学杂志, 2006,29(4):246-248
doi: 10.3760/cma.j.issn.1673-4386.2006.04.002
[14] Konishi H, Katoh Y, Takaya N , et al. Platelets activated by collagen through immunoreceptor tyrosine-based activation motif play pivotal role in initiation and generation of neointimal hyperplasia after vascular injury[J]. Circulation, 2002,105(8):912-916
doi: 10.1161/hc0802.105256
No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228