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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (6): 453-455.doi: 10.3736/jcim20040616

• Original Experimental Research • Previous Articles     Next Articles

Effect of acupuncture serum on intracellular Ca2+ concentration in cultured neurons

Rui-wu Li, Jin-ling Zhang, Ying Guo, Cui-hong Li   

  1. Institute of Acupuncture-Moxibustion, China Academy of Traditional Chinese Medicine, Beijing 100700, China
  • Received:2004-06-08 Online:2004-11-20 Published:2018-10-20

Objective: To study the humoral factor of acupuncture in improving functional activities of the organism.Methods: Neurons of the cerebral cortex from the new born rats were taken to be cultured in a strictly controlled medium. Seven to ten days later, fluorescein-molecular probe Fluo-3AM was used for staining intracellular Ca 2+. Ca 2+concentration and its changes after application of acupuncture serum were measured by using a laser confocal microscope. Normal serum and acupuncture serum were derived from the normal control rats and the rats undergoing electro-acupuncture of "Baihui (GV 20)", "Zusanli (ST 36)", "Quchi (LI 11)" and "Sanyinjiao (SP 6)" for 2 weeks. Results: After application of normal serum, the intracellular Ca 2+concentration increased strikingly immediately at the beginning, and gradually got stable afterwards. When acupuncture serum was added to the culture fluid, Ca 2+concentration lowered significantly. Conclusion: These results suggest that bioactive substances in serum collected from rats undergoing acupuncture can affect the intracellular Ca 2+ concentration of the cerebral neurons, and the humoral factor may participate in the effect of acupuncture in regulating functional activities of the organism.

Key words: acupuncture, serology, nerve cells, Ca2+

CLC Number: 

  • R245

Tab 1

Changes of fluorescence intensity of labeled intracellular Ca2+in cultured nerve cells after application of normal serum and acupuncture serum ($\bar{x}$±s )"

Time n Fluorescence intensity (Units)
Application of normal serum Application of acupuncture serum
Before application 10 10.04±3.23 22.66±3.69
50 s after application 10 28.08±10.34** 15.62±5.43**△△
150 s after application 10 23.82±8.40** 14.48±5.81**△△
300 s after application 10 23.52±4.66** 13.84±5.14**△△

Fig 1

Changes of intracellular Ca2+concentration in cultured nerve cells after application of normal serum and acupuncture serum In the coordinate figure, the left upright blue line shows the time of application of normal serum, and the right upright line indicates the time of application of acupuncture serum."

Fig 2

Original dynamic images of intracellular Ca2+before and after adding serum The Arabic numerals in this figure represent time (seconds); the normal serum was applied to the medium at the 60th second, and the acupuncture serum applied at the 500th second."

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