[an error occurred while processing this directive]

Journal of Integrative Medicine ›› 2014, Vol. 12 ›› Issue (1): 35-41.doi: 10.1016/S2095-4964(14)60001-7

• Research Article • Previous Articles     Next Articles

Protective effect of diosmin against diabetic neuropathy in experimental rats

Dilpesh Jaina, Manish Kumar Bansala, Rahul Dalvia, Aman Upganlawarb, Rahul Somanic   

  1. a Department of Pharmacology, Sinhgad College of Pharmacy, Vadgaon (Bk.), Pune 411041, India
    b Department of Pharmacology, SSDJ College of Pharmacy, Chandwad, Nashik 423101, India
    c Department of Research, Suresh Gyan Vihar University, Mahal Jagtpura, Jaipur 302025, India
  • Received:2013-08-12 Accepted:2013-10-28 Online:2014-01-10 Published:2014-01-15

RichHTML

PDF (PC)

341

Cited

83

Like

Objective

The present study was undertaken to evaluate the effect of diosmin in diabetic neuropathy in type 2 diabetic rats. 

Methods

Type 2 diabetes was induced in male Sprague-Dawley rats by single intraperitoneal injection of streptozotocin (35 mg/kg) and high-fat diet. Four weeks after the confirmation of diabetes, diabetic rats were treated with diosmin (50 and 100 mg/kg, p.o.) for next 4 weeks. Rats were evaluated for biochemical, behavioral and oxidative stress parameters. Eddy’s hot plate and tail immersion test were performed on 6th, 7th, 8th, 9th and 10th weeks of experiment to assess thermal hyperalgesia and cold allodynia respectively. Further, the walking function test was performed for assessing the motor responses at the end of the treatment schedule. 

Results

Rats were fed with high-fat diet throughout the experiment schedule and administration of low-dose streptozotocin induced significant elevation in blood glucose level and insulin resistance which was confirmed by oral glucose tolerance test. Treatment with diosmin at doses of 50 and 100 mg/kg significantly restored the reduced body weight, elevated blood sugar and lipid profiles. Further the dose-dependent improvement was observed in thermal hyperalgesia, cold allodynia and walking function in diabetic rats treated with diosmin. Elevated levels of malondialdehyde, and nitric oxide and decreased glutathione levels and superoxide dismutase activity in diabetic rats were restored significantly after the 4 weeks of diosmin treatment. 

Conclusion

Diosmin has shown beneficial effect in preventing the progression of early diabetic neuropathy in rats.

Key words: Diosmin, plant extracts, Diabetic neuropathies, Diabetes mellitus, Type 2, Rats

Figure 1

Oral glucose tolerance test curves of normal rats and HFE- and HFD-fed rats **P<0.01, vs normal control rats.NC: normal control; HFD: high-fat diet; HFE: high-fat emulsion."

"

"

Figure 2

Effects of diosmin on thermal hyperalgesiaData are expressed as mean ± standard error of mean, n=6; **P < 0.01, vs normal control rats; △△P < 0.01, vs diabetic control rats.NC: normal control; DC: diabetic control."

Figure 3

Effects of diosmin on tail flick latency Data are expressed as mean ± standard error of mean, n=6; **P < 0.01, vs normal control rats; △△P < 0.01, vs diabetic control rats.NC: normal control; DC: diabetic control."

Figure 4

Effects of diosmin on walking function test Data are expressed as mean ± standard error of mean, n=6; **P < 0.01, vs normal control rats; △△P < 0.01, vs diabetic control rats.NC: normal control; DC: diabetic control."

"

"

[1] Maiti R, Das UK, Ghosh D . Attenuation of hyperglycemia and hyperlipidemia in streptozotocin-induced diabetic rats by aqueous extract of seed of Tamarindus indica[J]. Biol Pharm Bull, 2005,28(7):1172-1176
doi: 10.1248/bpb.28.1172
[2] Guyton AC, Hall E . Text book of medical physiology.10th ed[M]. New Delhi: Elsevier, 2000: 894-897
[3] Kaeidi A, Esmaeili-Mahani S, Sheibani V, Abbasnejad M, Rasoulian B, Hajializadeh Z, Afrazi S . Olive(Olea europaea L.) leaf extract attenuates early diabetic neuropathic pain through prevention of high glucose-induced apoptosis: in vitro and in vivo studies[J]. J Ethnopharmacol, 2011,136(1):188-196
doi: 10.1016/j.jep.2011.04.038
[4] Yagihashi S, Yamagishi S, Wada R . Pathology and pathogenetic mechanisms of diabetic neuropathy: correlation with clinical signs and symptoms[J]. Diabetes Res Clin Pract, 2007,77(Suppl 1):S184-S189
doi: 10.1002/dmr.5610110304 pmid: 8536541
[5] Lehmann R, Schleicher ED . Molecular mechanisms of diabetic neuropathy[J]. Clin Chim Acta, 2000,297(1-2):135-144
doi: 10.1016/S0009-8981(00)00240-0 pmid: 10841915
[6] Ziegler D. Treatment of diabetic neuropathy and neuropathic pain: how far have we come? Diabetes Care. 2008; 31(Suppl 2):S255-S261.
doi: 10.2337/dc08-s263
[7] Courteix C, Bardin M, Chantelauze C, Lavarenne J, Eschalier A . Study of the sensitivity of the diabetes-induced pain model in rats to a range of analgesics[J]. Pain, 1994,57(2):153-160
doi: 10.1016/0304-3959(94)90218-6 pmid: 8090511
[8] Gosavi TP, Ghosh P, Kandhare A, Bodhankar SL . Unwrapping homeopathic principles in the wake of research: serendipity, placebo or true therapeutic milestones[J]. Pharmacologynline, 2011,1:894-906
[9] Cova D, De Angelis L, Giavarini F, Palladini G, Perego R . Pharmacokinetics and metabolism of oral diosmin in healthy volunteers[J]. Int J Clin Pharmacol Ther Toxicol, 1992,30(1):29-33
doi: 10.3109/08923979209009244 pmid: 1551741
[10] Smith PD . Neutrophil activation and mediators of inflammation in chronic venous insufficiency[J]. J Vasc Res, 1999,36(Suppl 1):24-36
doi: 10.1159/000054071
[11] Srinivasan S, Pari L . Ameliorative effect of diosmin, a citrus flavonoid against streptozotocin-nicotinamide generated oxidative stress induced diabetic rats[J]. Chem Biol Interact, 2012,195(1):43-51
doi: 10.1016/j.cbi.2011.10.003
[12] Xu D, Xu M, Lin L, Rao S, Wang J, Davey AK . The effect of isosteviol on hyperglycemia and dyslipidemia induced by lipotoxicity in rats fed with high-fat emulsion[J]. Life Sci, 2012,90(1-2):30-38
doi: 10.1016/j.lfs.2011.10.010
[13] Srinivasan K, Viswanad B, Asrat L, Kaul CL, Ramarao P . Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: a model for type 2 diabetes and pharmacological screening[J]. Pharmacol Res, 2005,52(4):313-320
doi: 10.1016/j.phrs.2005.05.004
[14] Kastrup J, Petersen P, Dejg?rd A, Angelo HR, Hilsted J. Intravenous lidocaine infusion — a new treatment of chronic painful diabetic neuropathy? Pain. 1987; 28(1):69-75.
doi: 10.1016/0304-3959(87)91061-X
[15] Wybenga DR, Pileggi VJ, Dirstine PH, Di Giorgio J . Direct manual determination of serum total cholesterol with a single stable reagent[J]. Clin Chem, 1970,16(12):980-984
doi: 10.1016/0273-1177(94)90201-1 pmid: 4098216
[16] Bucolo G, David H . Quantitative determination of serum triglycerides by the use of enzymes[J]. Clin Chem, 1973,19(5):476-482
pmid: 4703655
[17] Gornall AG, Bardawill CJ, David MM . Determination of serum proteins by means of the biuret reaction[J]. J Biol Chem, 1949,177(2):751-766
pmid: 18110453
[18] Kuhad A, Sharma S, Chopra K . Lycopene attenuates thermal hyperalgesia in a diabetic mouse model of neuropathic pain[J]. Eur J Pain, 2008,12(5):624-632
doi: 10.1016/j.ejpain.2007.10.008 pmid: 18055235
[19] Bhutada P, Mundhada Y, Humane V, Rahigude A, Deshmukh P, Latad S, Jain K . Agmatine, an endogenous ligand of imidazoline receptor protects against memory impairment and biochemical alterations in streptozotocin-induced diabetic rats[J]. Prog Neuropsychopharmacol Biol Psychiatry, 2012,37(1):96-105
doi: 10.1016/j.pnpbp.2012.01.009
[20] Kunkel-Bagden E, Bregman BS . Spinal cord transplants enhance the recovery of locomotor function after spinal cord injury at birth[J]. Exp Brain Res, 1990,81(1):25-34
[21] Mihara M, Uchiyama M . Determination of malonaldehyde precursor in tissues by thiobarbituric acid test[J]. Anal Biochem, 1978,86(1):271-278
doi: 10.1016/0003-2697(78)90342-1
[22] Ellman GL . Tissue sulfhydryl groups[J]. Arch Biochem Biophys, 1959,82(1):70-77
doi: 10.1016/0003-9861(59)90090-6
[23] Kono Y . Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase[J]. Arch Biochem Biophys, 1978,186(1):189-195
doi: 10.1016/0003-9861(78)90479-4
[24] Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR . Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids[J]. Anal Biochem, 1982,126(1):131-138
doi: 10.1016/0003-2697(82)90118-X pmid: 7181105
[25] Whiting DR, Guariguata L, Weil C, Shaw J . IDF diabetes atlas: global estimates of the prevalence of diabetes for 2011 and 2030[J]. Diabetes Res Clin Pract, 2011,94(3):311-321
doi: 10.1016/j.diabres.2011.10.029 pmid: 22079683
[26] Sezer A, Usta U, Kocak Z, Yagci MA . The effect of a flavonoid fractions diosmin + hesperidin on radiation-induced acute proctitis in a rat model[J]. J Cancer Res Ther, 2011,7(2):152-156
doi: 10.4103/0973-1482.82927
[27] Head KA . Peripheral neuropathy: pathogenic mechanisms and alternative therapies[J]. Altern Med Rev, 2006,11(4):294-329
doi: 10.1016/j.jep.2006.06.006 pmid: 17176168
[28] Pari L, Srinivasan S . Antihyperglycemic effect of diosmin on hepatic key enzymes of carbohydrate metabolism in streptozotocin-nicotinamide-induced diabetic rats[J]. Biomed Pharmacother, 2010,64(7):477-481
doi: 10.1016/j.biopha.2010.02.001
[29] Kitto KF, Haley JE, Wilcox GL . Involvement of nitric oxide in spinally mediated hyperalgesia in the mouse[J]. Neurosci Lett, 1992,148(1-2):1-5
doi: 10.1016/0304-3940(92)90790-E pmid: 1284437
[30] Calcutt NA, Jorge MC, Yaksh TL, Chaplan SR . Tactile allodynia and formalin hyperalgesia in streptozotocin-diabetic rats: effects of insulin, aldose reductase inhibition and lidocaine[J]. Pain, 1996,68(2-3):293-299
doi: 10.1016/S0304-3959(96)03201-0
[31] Muller KA, Ryals JM, Feldman EL, Wright DE . Abnormal muscle spindle innervation and large-fiber neuropathy in diabetic mice[J]. Diabetes, 2008,57(6):1693-1701
doi: 10.2337/db08-0022 pmid: 18362211
[32] Kim SY, Lee JH, Yang ES, Kil IS, Park JW . Human sensitive to apoptosis gene protein inhibits peroxynitrite-induced DNA damage[J]. Biochem Biophys Res Commun, 2003,301(3):671-674
doi: 10.1016/S0006-291X(03)00018-4 pmid: 12565832
[1] Wan-jun Guo, Yi Wang, Yu Deng, Lin-yan Cheng, Xin Liu, Ruo-fan Xi, Sheng-jie Zhu, Xin-yi Feng, Liang Hua, Kan Ze, Jian-yong Zhu, Dong-jie Guo, Fu-lun Li. Therapeutic effects of the extract of Sancao Formula, a Chinese herbal compound, on imiquimod-induced psoriasis via cysteine-rich protein 61. Journal of Integrative Medicine, 2022, 20(4): 376-384.
[2] Keat Lam Ho, Phaik Har Yong, Chee Woon Wang, Umah Rani Kuppusamy, Chek Tung Ngo, Festo Massawe, Zhi Xiang Ng. Peperomia pellucida (L.) Kunth and eye diseases: A review on phytochemistry, pharmacology and toxicology. Journal of Integrative Medicine, 2022, 20(4): 292-304.
[3] Lu Yin, Yun An, Xiao Chen, Hui-xin Yan, Tao Zhang, Xin-gang Lu, Jun-tao Yan. Local vibration therapy promotes the recovery of nerve function in rats with sciatic nerve injury. Journal of Integrative Medicine, 2022, 20(3): 265-273.
[4] Zeinab Vafaeipour, Bibi Marjan Razavi, Hossein Hosseinzadeh. Effects of turmeric (Curcuma longa) and its constituent (curcumin) on the metabolic syndrome: An updated review. Journal of Integrative Medicine, 2022, 20(3): 193-203.
[5] Marisa Casal. Improving the health and treatment success rates of in vitro fertilization patients with traditional chinese medicine: need for more robust evidence and innovative approaches. Journal of Integrative Medicine, 2022, 20(3): 187-192.
[6] Ning Guo, Fei Wu, Mei Wu, Yuan Wang, Qing Lang, Xiao Lin, Yi Feng. Progress in the design and quality control of placeboes for clinical trials of traditional Chinese medicine . Journal of Integrative Medicine, 2022, 20(3): 204-212.
[7] Jia-yi Zhao, Jin Pu, Jian Fan, Xin-yu Feng, Jian-wen Xu, Rong Zhang, Yan Shang. Tanshinone IIA prevents acute lung injury by regulating macrophage polarization. Journal of Integrative Medicine, 2022, 20(3): 274-280.
[8] Yan-jun Lin, Kun-li Jiao, Bo Liu, Lu Fang, Shu Meng. Antiplatelet and myocardial protective effect of shexiang tongxin dropping pill in patients undergoing percutaneous coronary intervention: A randomized controlled trial. Journal of Integrative Medicine, 2022, 20(2): 126-134.
[9] Peter Bablis, Henry Pollard, Anthony L. Rosner. Stress reduction via neuro-emotional technique to achieve the simultaneous resolution of chronic low back pain with multiple inflammatory and biobehavioural indicators: A randomized, double-blinded, placebo-controlled trial. Journal of Integrative Medicine, 2022, 20(2): 135-144.
[10] Jose Antonio Castilla-Jimena, Isabel Ruiz-Pérez, Jesús Henares-Montiel. Impact of socioeconomic and health-related factors on consumption of homeopathic and natural remedies in Spain in 2006, 2011 and 2017. Journal of Integrative Medicine, 2022, 20(1): 52-56.
[11] Jun-peng Yao, Li-ping Chen, Xian-jun Xiao, Ting-hui Hou, Si-yuan Zhou, Ming-min Xu, Kai Wang, Yu-jun Hou, Lin Zhang, Ying Li. Effectiveness and safety of acupuncture for treating functional constipation: An overview of systematic reviews. Journal of Integrative Medicine, 2022, 20(1): 13-25.
[12] Ofer Baranovitch, Meirav Wolff-Bar, Meora Feinmesser, Chen Sade-Zaltz, Ilan Tsarfaty, Victoria Neiman. Searching for the emotional roots of breast cancer: A cross-disciplinary analysis integrating psychology, Chinese medicine, and oncology biomarkers. Journal of Integrative Medicine, 2022, 20(1): 57-64.
[13] Deng-chao Wang, Miao Yu, Wen-xian Xie, Li-yan Huang, Jian Wei, Yue-hua Lei. Meta-analysis on the effect of combining Lianhua Qingwen with Western medicine to treat coronavirus disease 2019. Journal of Integrative Medicine, 2022, 20(1): 26-33.
[14] Miriam Bazzicalupo, Laura Cornara, Bruno Burlando, Alberta Cascini, Marcella Denaro, Antonella Smeriglio, Domenico Trombetta. Carpobrotus edulis (L.) N.E.Br. extract as a skin preserving agent: from traditional medicine to scientific validation. Journal of Integrative Medicine, 2021, 19(6): 526-536.
[15] Meng-ya Shan, Ying Dai, Xiao-dan Ren, Jing Zheng, Ke-bin Zhang, Bin Chen, Jun Yan, Zi-hui Xu. Berberine mitigates nonalcoholic hepatic steatosis by downregulating SIRT1-FoxO1-SREBP2 pathway for cholesterol synthesis. Journal of Integrative Medicine, 2021, 19(6): 545-554.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
[an error occurred while processing this directive]