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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (1): 77-82.doi: 10.3736/jcim20080115

• Original Experimental Research • Previous Articles     Next Articles

Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice

A-gao Zhou1(), Yong Zhang2, Gang Kui1, De-Yun Kong3, Hai-liang Ge2, Qiu-hua Ren2, Jia-rong Dong1, Sheng Hong1, Xu-ming Mao1, Yin Wang2, Hui-zheng Zhang2, Shu-jun Wang2   

  1. 1. Department of Traditional Chinese Medicine, Ninth People s Hospital, School of Medicine, Shanghai Jiao tong University, Shanghai 200011, China
    2. Shanghai Institute of Immunology, Shanghai 200025, China
    3. Department of Traditional Chinese Drug, Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China
  • Received:2007-07-04 Online:2008-01-20 Published:2008-01-15
  • Contact: ZHOU A-gao

Objective:To compare the influence of traditional Chinese compound recipes (TCCRs) with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice.

Methods:H22 cancer-bearing mice were chosen to observe the effects of TCCRs with different efficacy on tumor growth inhibition and detect the proliferation function of T lymphocytes, the activity of natural killer (NK) cells, the changes of T lymphocytes and the content of interferon-γ (IFN-γ)and interleukin-4 (IL-4).

Results:Tumor weight of H22 cancer-bearing mice in Yidu Gongdu Recipe (YDGDR, a compound traditional Chinese herbal medicine using poison as an antidote for poison)-treated group was obviously lighter than that in the other TCCR-treated groups and the tumor inhibition rate in YDGDR-treated group was 65.76% (P<0.01). The tumor inhibition rates in other TCCR-treated groups were ranged from 10.1% to 17.1%. Body weight of mice in YDGDR-treated group was obviously decreased and depilation was observed at the same time. Pelage of mice in Fuzheng Peiben Recipe (FZPBR, a compound traditional Chinese herbal medicine for supporting the healthy energy)-treated group grew well, and behavior of the mice was active. Stimulation index (SI) of T lymphocyte transformation in YDGDR-treated group was obviously increased (SI=4.34, P<0.01), which showed the proliferation function of T lymphocyte was very strong. The SI of T lymphocyte transformation in the other groups was less than three, which showed the proliferation function of T lymphocytes was not significant. Compared with normal saline (NS)-treated group, percentages of NK cells in Qinre Jiedu Recipe (QRJDR, a compound traditional Chinese herbal medicine for clearing away heat and toxic substances)-treated, Huxue Huayu Recipe (HXHYR, a compound traditional Chinese herbal medicine for activating blood circulation to dissipate blood stasis)-treated and YDGDR-treated groups were obviously increased and 5.05, 4.07 and 5.17 times more than the NS-treated group, respectively (P<0.01). The activity of NK cells wasn't increased in the FZPBR-treated and HXHYR-treated groups. The production of IFN-γ induced by T cells in YDGDR-treated group was obviously raised (P<0.05), and the production of IL-4 induced by T cells in QRJDR-treated, HXHYR-treated, Huatan Sanjie Recipe (a compound traditional Chinese herbal medicine for eliminating phlegm and resolving masses)-treated and YDGDR-treated groups was also raised obviously (P<0.01).

Conclusion:YDGDR has a good effect of inhibiting tumor growth and can reinforce cellular and humoral immune function in tumor-bearing mice. FZPBR can strengthen the body.

Key words: therapeutic methods, traditional Chinese medicine, composite (traditional Chinese drugs), liver cancer, body weight, immunity, mice

CLC Number: 

  • R735.7

Table 1

Influence of traditional Chinese compound recipes with different effects on body weight and tumour weight of mice ($\bar{x}$±s)"

Group n Body weight (g) Tumor weight (g) Tumor inhibition (%)
Normal 10 24.51±2.75 - -
NS-treated 10 22.62±2.59 3.68±1.08 -
Fuzheng Peiben-treated 10 23.97±3.35 3.26±1.37 11.4
Qingre Jiedu-treated 10 23.94±0.43 3.31±1.74 10.1
Huoxue Huayu-treated 10 22.23±2.54 3.05±1.48 17.1
Huatan Sanjie-treated 10 24.30±2.52 3.07±0.94 16.6
Yidu Gongdu-treated 10 19.06±1.28△△ 1.26±0.67** 65.8

Table 2

Influence of traditional Chinese compound recipes with different effects on proliferation of T cells in tumor-bearing mice ($\bar{x}$±s)"

Group n SI
Normal 10 42.01±5.78
NS-treated 10 1.43±0.71△△
Fuzheng Peiben-treated 10 1.67±0.58△△
Qingre Jiedu-treated 10 1.69±0.26△△
Huoxue Huayu-treated 10 1.69±0.86△△
Huatan Sanjie-treated 10 1.88±0.74△△
Yidu Gongdu-treated 10 4.34±2.27**△△

Table 3

Influence of traditional Chinese compound recipes with different effects on changes of NK cells and T cell subsets in tumor-bearing mice ($\bar{x}$±s)"

Group n NK CD3+ CD4+ CD8+ CD4+/CD8+
Normal 10 0.102±0.020 28.67±3.11 15.75±1.56 14.53±1.99 1.08
NS-treated 10 0.115±0.066 20.43±3.48* 9.33±1.95** 11.66±1.94* 0.80
Fuzheng Peiben-treated 10 0.079±0.041 14.94±6.02* 7.82±3.18** 7.52±3.27* 1.04
Qingre Jiedu-treated 10 0.581±0.187** 19.51±0.95△ 11.88±0.50△ 8.95±1.09△ 1.33
Huoxue Huayu-treated 10 0.468±0.095** 20.78±3.10△ 11.70±1.86△ 10.27±1.51△ 1.14
Huatan Sanjie-treated 10 0.111±0.017 17.34±3.74△ 8.71±2.05△△ 9.65±2.37△ 0.90
Yidu Gongdu-treated 10 0.595±0.187** 22.82±4.07△ 11.13±1.64△ 13.49±2.79△ 0.83

Table 4

Influence of traditional Chinese compound recipes with different effects on excretion of IFN-γ and IL-4 in tumor-bearing mice ($\bar{x}$±s, ng/L)"

Group n IFN-γ IL-4
Normal 10 47.61±20.50 109.11±18.80
NS-treated 10 43.91±20.83 125.82±10.66
Fuzheng Peiben-treated 10 23.61±17.01* 102.46±16.71
Qingre Jiedu-treated 10 31.94±6.51 297.93±21.93**
Huoxue Huayu-treated 10 46.35±21.68 339.21±31.18**
Huatan Sanjie-treated 10 24.39±9.77* 291.43±8.18**
Yidu Gongdu-treated 10 99.68±37.88** 301.82±11.27**
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