SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (1): 77-82.doi: 10.3736/jcim20080115

• Original Experimental Research • Previous Articles     Next Articles

Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice

A-gao Zhou1(), Yong Zhang2, Gang Kui1, De-Yun Kong3, Hai-liang Ge2, Qiu-hua Ren2, Jia-rong Dong1, Sheng Hong1, Xu-ming Mao1, Yin Wang2, Hui-zheng Zhang2, Shu-jun Wang2   

  1. 1. Department of Traditional Chinese Medicine, Ninth People s Hospital, School of Medicine, Shanghai Jiao tong University, Shanghai 200011, China
    2. Shanghai Institute of Immunology, Shanghai 200025, China
    3. Department of Traditional Chinese Drug, Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China
  • Received:2007-07-04 Online:2008-01-20 Published:2008-01-15
  • Contact: ZHOU A-gao E-mail:zagssmu@sina.com

RichHTML

2

PDF (PC)

63

Like

Objective:To compare the influence of traditional Chinese compound recipes (TCCRs) with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice.

Methods:H22 cancer-bearing mice were chosen to observe the effects of TCCRs with different efficacy on tumor growth inhibition and detect the proliferation function of T lymphocytes, the activity of natural killer (NK) cells, the changes of T lymphocytes and the content of interferon-γ (IFN-γ）and interleukin-4 (IL-4).

Results:Tumor weight of H22 cancer-bearing mice in Yidu Gongdu Recipe (YDGDR, a compound traditional Chinese herbal medicine using poison as an antidote for poison)-treated group was obviously lighter than that in the other TCCR-treated groups and the tumor inhibition rate in YDGDR-treated group was 65.76% (P<0.01). The tumor inhibition rates in other TCCR-treated groups were ranged from 10.1％ to 17.1%. Body weight of mice in YDGDR-treated group was obviously decreased and depilation was observed at the same time. Pelage of mice in Fuzheng Peiben Recipe (FZPBR, a compound traditional Chinese herbal medicine for supporting the healthy energy)-treated group grew well, and behavior of the mice was active. Stimulation index (SI) of T lymphocyte transformation in YDGDR-treated group was obviously increased (SI=4.34, P<0.01), which showed the proliferation function of T lymphocyte was very strong. The SI of T lymphocyte transformation in the other groups was less than three, which showed the proliferation function of T lymphocytes was not significant. Compared with normal saline (NS)-treated group, percentages of NK cells in Qinre Jiedu Recipe (QRJDR, a compound traditional Chinese herbal medicine for clearing away heat and toxic substances)-treated, Huxue Huayu Recipe (HXHYR, a compound traditional Chinese herbal medicine for activating blood circulation to dissipate blood stasis)-treated and YDGDR-treated groups were obviously increased and 5.05, 4.07 and 5.17 times more than the NS-treated group, respectively (P<0.01). The activity of NK cells wasn't increased in the FZPBR-treated and HXHYR-treated groups. The production of IFN-γ induced by T cells in YDGDR-treated group was obviously raised (P<0.05), and the production of IL-4 induced by T cells in QRJDR-treated, HXHYR-treated, Huatan Sanjie Recipe (a compound traditional Chinese herbal medicine for eliminating phlegm and resolving masses)-treated and YDGDR-treated groups was also raised obviously (P<0.01).

Conclusion:YDGDR has a good effect of inhibiting tumor growth and can reinforce cellular and humoral immune function in tumor-bearing mice. FZPBR can strengthen the body.

Key words: therapeutic methods, traditional Chinese medicine, composite (traditional Chinese drugs), liver cancer, body weight, immunity, mice

CLC Number: 

  • R735.7

Table 1

Influence of traditional Chinese compound recipes with different effects on body weight and tumour weight of mice ($\bar{x}$±s)"

Group n Body weight (g) Tumor weight (g) Tumor inhibition (%)
Normal 10 24.51±2.75 - -
NS-treated 10 22.62±2.59 3.68±1.08 -
Fuzheng Peiben-treated 10 23.97±3.35 3.26±1.37 11.4
Qingre Jiedu-treated 10 23.94±0.43 3.31±1.74 10.1
Huoxue Huayu-treated 10 22.23±2.54 3.05±1.48 17.1
Huatan Sanjie-treated 10 24.30±2.52 3.07±0.94 16.6
Yidu Gongdu-treated 10 19.06±1.28△△ 1.26±0.67** 65.8

Table 2

Influence of traditional Chinese compound recipes with different effects on proliferation of T cells in tumor-bearing mice ($\bar{x}$±s)"

Group n SI
Normal 10 42.01±5.78
NS-treated 10 1.43±0.71△△
Fuzheng Peiben-treated 10 1.67±0.58△△
Qingre Jiedu-treated 10 1.69±0.26△△
Huoxue Huayu-treated 10 1.69±0.86△△
Huatan Sanjie-treated 10 1.88±0.74△△
Yidu Gongdu-treated 10 4.34±2.27**△△

Table 3

Influence of traditional Chinese compound recipes with different effects on changes of NK cells and T cell subsets in tumor-bearing mice ($\bar{x}$±s)"

Group n NK CD3+ CD4+ CD8+ CD4+/CD8+
Normal 10 0.102±0.020 28.67±3.11 15.75±1.56 14.53±1.99 1.08
NS-treated 10 0.115±0.066 20.43±3.48* 9.33±1.95** 11.66±1.94* 0.80
Fuzheng Peiben-treated 10 0.079±0.041 14.94±6.02* 7.82±3.18** 7.52±3.27* 1.04
Qingre Jiedu-treated 10 0.581±0.187** 19.51±0.95△ 11.88±0.50△ 8.95±1.09△ 1.33
Huoxue Huayu-treated 10 0.468±0.095** 20.78±3.10△ 11.70±1.86△ 10.27±1.51△ 1.14
Huatan Sanjie-treated 10 0.111±0.017 17.34±3.74△ 8.71±2.05△△ 9.65±2.37△ 0.90
Yidu Gongdu-treated 10 0.595±0.187** 22.82±4.07△ 11.13±1.64△ 13.49±2.79△ 0.83

Table 4

Influence of traditional Chinese compound recipes with different effects on excretion of IFN-γ and IL-4 in tumor-bearing mice ($\bar{x}$±s, ng/L)"

Group n IFN-γ IL-4
Normal 10 47.61±20.50 109.11±18.80
NS-treated 10 43.91±20.83 125.82±10.66
Fuzheng Peiben-treated 10 23.61±17.01* 102.46±16.71
Qingre Jiedu-treated 10 31.94±6.51 297.93±21.93**
Huoxue Huayu-treated 10 46.35±21.68 339.21±31.18**
Huatan Sanjie-treated 10 24.39±9.77* 291.43±8.18**
Yidu Gongdu-treated 10 99.68±37.88** 301.82±11.27**
[1] Tang HJ, Gao SP, Zheng Y , et al. Clinical observation of 288 cases treated by Chinese medicine after surgery of mammary cancer. Shanghai Zhong Yi Yao Da Xue Xue Bao. 2002; 16(3):23-25. Chinese with abstract in English.
doi: 10.3969/j.issn.1008-861X.2002.03.008
唐汉钧, 髙尚璞, 郑勇 , 等. 中医药治疗乳腺癌术后288 例临床观察.上海中医药大学学报. 2002; 16(3):23-25.
doi: 10.3969/j.issn.1008-861X.2002.03.008
[2] Sun TZ, Zheng YL, Wang XQ. Influence of Qirui Fuzheng Capsule on activities of IL-2, NK cells and T cell subgroups in lung cancer. Zhong Yi Yao Xue Kan. 2004; 22(12):2229-2230. Chinese.
doi: 10.3969/j.issn.1673-7717.2004.12.033
孙太振, 郑玉玲, 王祥麒 . 芪瑞扶正胶囊对肺癌IL-2､ NK细胞活性和T细胞亚群的影响.中医药学刊. 2004; 22(12):2229-2230.
doi: 10.3969/j.issn.1673-7717.2004.12.033
[3] Zhou AG, Huang DW, Ding YX , et al. Clinical and experimental studies on treatment postoperative gastric cancer with combined therapy of Fuzhen Huoxue anticancer prescription and chemotherapy. Zhongguo Zhong Xi Yi Jie He Za Zhi. 1996; 16(9):519-521. Chinese with abstract in English.
周阿髙, 黄大慰, 丁钰熊 , 等. 扶正活血抗癌方合并化疗治疗胃癌(术后)的临床与实验研究.中国中西医结合杂志. 1996; 16(9):519-521.
[4] Zhong Y , Zhou RY, Wu LY. Clinical observation on the effect of Fuzheng Ruanjian Decoction in treating 60 cases of progressive cancer patients. Liaoning Zhong Yi Za Zhi. 2003 ; 2003; 30(11):913-914. Chinese with abstract in English.
doi: 10.3969/j.issn.1000-1719.2003.11.031
钟薏, 周荣耀, 吴丽英 . 扶正软坚汤治疗晚期恶性肿瘤 60例临床观察.辽宁中医杂志 . 2003; 30(11):913-914.
doi: 10.3969/j.issn.1000-1719.2003.11.031
[5] Li DT , Sun GZ. Changes of serum immuno-cellular factors in patients of hepatocarcinoma treated with the therapy of Yiqi Huoxue Ruanjian Jiedu decoction. Zhongguo Zhong Xi Yi Jie He Xiao Hua Za Zhi. 2005; 13(4):226-228, 232. Chinese with abstract in English.
doi: 10.3969/j.issn.1671-038X.2005.04.005
李东涛, 孙桂芝 . 益气活血软坚解毒法对肝癌患者免疫因子的影响.中国中西医结合消化杂志. 2005; 13(4):226-228, 232.
doi: 10.3969/j.issn.1671-038X.2005.04.005
[6] Chen Z, Zhai XF, Su YH , et al. Clinical observation of cinobuf acini injection used to treat moderate and advanced primary liver cancer. Zhong Xi Yi Jie He Xue Bao. 2003; 1(3):184-186. Chinese with abstract in English.
陈喆, 翟笑枫, 苏永华 , 等. 华蟾素注射液治疗中晚期原发性肝癌临床疗效观察.中西医结合学报. 2003; 1(3):184-186.
[7] Liu HT , Dai XM. Rearch on effect and mechanism of inhibiting tumor by Fuzheng decoction on LA795 metastatic lung cancer mice. Jiangsu Zhong Yi Yao. 2004; 25(10):56-58. Chinese.
刘海涛, 戴锡孟 . 中药扶正合剂对LA795转移性肺癌小鼠的抑瘤作用和机制研究.江苏中医药. 2004; 25(10):56-58.
[1] Wan-jun Guo, Yi Wang, Yu Deng, Lin-yan Cheng, Xin Liu, Ruo-fan Xi, Sheng-jie Zhu, Xin-yi Feng, Liang Hua, Kan Ze, Jian-yong Zhu, Dong-jie Guo, Fu-lun Li. Therapeutic effects of the extract of Sancao Formula, a Chinese herbal compound, on imiquimod-induced psoriasis via cysteine-rich protein 61. Journal of Integrative Medicine, 2022, 20(4): 376-384.
[2] Shang-jin Song, Xuan Liu, Qing Ji, Da-zhi Sun, Li-juan Xiu, Jing-yu Xu, Xiao-qiang Yue. Ziyin Huatan Recipe, a Chinese herbal compound, inhibits migration and invasion of gastric cancer by upregulating RUNX3 expression. Journal of Integrative Medicine, 2022, 20(4): 355-364.
[3] Marisa Casal. Improving the health and treatment success rates of in vitro fertilization patients with traditional chinese medicine: need for more robust evidence and innovative approaches. Journal of Integrative Medicine, 2022, 20(3): 187-192.
[4] Ning Guo, Fei Wu, Mei Wu, Yuan Wang, Qing Lang, Xiao Lin, Yi Feng. Progress in the design and quality control of placeboes for clinical trials of traditional Chinese medicine . Journal of Integrative Medicine, 2022, 20(3): 204-212.
[5] Yan-jun Lin, Kun-li Jiao, Bo Liu, Lu Fang, Shu Meng. Antiplatelet and myocardial protective effect of shexiang tongxin dropping pill in patients undergoing percutaneous coronary intervention: A randomized controlled trial. Journal of Integrative Medicine, 2022, 20(2): 126-134.
[6] Gai Ran, Xi-lin Feng, Yi-lin Xie, Qing-yun Zheng, Peng-peng Guo, Ming Yang, Ying-lu Feng, Chen Ling, Li-qing Zhu, Chen Zhong. The use of miR122 and its target sequence in adeno-associated virus-mediated trichosanthin gene therapy. Journal of Integrative Medicine, 2021, 19(6): 515-525.
[7] Jia-jia Li, Qing Liang, Guang-chun Sun. Traditional Chinese medicine for prevention and treatment of hepatocellular carcinoma: A focus on epithelial-mesenchymal transition. Journal of Integrative Medicine, 2021, 19(6): 469-477.
[8] Hao Gou, Ruo-chen Huang, Yong-hua Su, Wei Li. Design of dual targeting immunomicelles loaded with bufalin and study of their anti-tumor effect on liver cancer. Journal of Integrative Medicine, 2021, 19(5): 408-417.
[9] Rui-jin Qiu, Min Li, Jia-yuan Hu, Jing Chen, Hong-cai Shang. Methods for development of a core outcome set for clinical trials integrating traditional Chinese medicine and Western medicine. Journal of Integrative Medicine, 2021, 19(5): 389-394.
[10] Liang Ding, Xin-you Zhang, Di-yao Wu, Meng-ling Liu. Application of an extreme learning machine network with particle swarm optimization in syndrome classification of primary liver cancer. Journal of Integrative Medicine, 2021, 19(5): 395-407.
[11] Victor Abou Nehmi Filho, Gilson Masahiro Murata, Ruan Carlos Macêdo de Moraes, Gabriely Cristina Alves Lima, Danielle Araujo de Miranda, Katrin Radloff, Raquel Galvão Figuerêdo Costa, Joyce de Cassia Rosa de Jesus, Jéssica Alves De Freitas, Nayara Izabel Viana, Ruan Pimenta, Katia Ramos Moreira Leite, José Pinhata Otoch, Ana Flávia Marçal Pessoa. A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice. Journal of Integrative Medicine, 2021, 19(5): 439-450.
[12] Mao-xing Pan, Chui-yang Zheng, Yuan-jun Deng, Kai-rui Tang, Huan Nie, Ji-qian Xie, Dong-dong Liu, Gui-fang Tu, Qin-he Yang, Yu-pei Zhang. Hepatic protective effects of Shenling Baizhu powder, a herbal compound, against inflammatory damage via TLR4/NLRP3 signalling pathway in rats with nonalcoholic fatty liver disease . Journal of Integrative Medicine, 2021, 19(5): 428-438.
[13] Ning Zhang, Xiao-he Xiao. Integrative medicine in the era of cancer immunotherapy: Challenges and opportunities. Journal of Integrative Medicine, 2021, 19(4): 291-294.
[14] Thomas Efferth, An-long Xu, Roxana Damiescu, Mita Banerjee, Norbert W. Paul, David Y.W. Lee. Can eastern wisdom resolve western epidemics? Traditional chinese medicine therapies and the opioid crisis. Journal of Integrative Medicine, 2021, 19(4): 295-299.
[15] Si-qi Tang, Yun-liang Wang, Zi-ye Xie, Yang Zhang, Yi Guo, Kang-li Gao, Tang-you Mao, Chun-e Xie, Jun-xiang Li, Xiao-yan Gao. Serum metabolic profiling of traditional Chinese medicine syndromes in patients with diarrhea-predominant irritable bowel syndrome. Journal of Integrative Medicine, 2021, 19(3): 274-281.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9] Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3