Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (1): 83-88.doi: 10.3736/jcim20080116

• Original Experimental Research • Previous Articles     Next Articles

Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice

Guo-hong Yuan1(), Xiao-jing Pang2, He-chao Ma2   

  1. 1. Department of General Surgery, Third Hospital, Peking University, Beijing 100038, China
    2. Department Basic Medical Sciences, Chengde Medical College, Chengde, Hebei Province 067000, China
  • Received:2007-03-20 Online:2008-01-20 Published:2008-01-15
  • Contact: YUAN Guo-hong

Objective: To study the inhibitory effect of Danggui Buxue Tang (DBT), a compound traditional Chinese herbal medicine for supplementing blood, on tumor growth in tumor-bearing mice after inoculation of EL-4 cells, and its immune mechanism as well as its synergic effect in reducing toxicity of cytoxan (CTX).Methods: Experiment was carried out in tumor-bearing mice after inoculation of EL-4 cells. The mice were randomly divided into four groups after 7 days of the inoculation: untreated group, DBT-treated group [24 g/(kg·d)], CTX-treated group [7.5 mg/(kg·d)] and DBT plus CTX-treated group, with another ten normal mice as control. Inhibitory rate of tumor growth, survival time, immune function and variability of blood cells were measured in the mice during the experiment.Results: After treatment of relevant interventions for 15 days, the tumor in the DBT-treated group, CTX-treated group and DBT plus CTX-treated group grew slower than the untreated group (P<0.05). Murine survival time in the DBT-treated group, CTX-treated group and DBT plus CTX-treated group was lengthened as compared with that in the untreated group (P<0.05). Compared with the untreated group, all kinds of immune indexes in the DBT-treated group and DBT plus CTX-treated group were significantly improved (P<0.05), while the immune indexes in the CTX-treated group were decreased (P<0.05). Compared with the CTX-treated group, all kinds of immune indexes in the DBT plus CTX-treated group were significantly improved (P<0.05).Conclusions: DBT can enhance the immune function in tumor-bearing mice and the inhibitory effect of DBT on tumor growth is related to the enhanced immune response. DBT can also increase the therapeutic effects and reduce the side effects of CTX.

Key words: antineoplastic agents, cyclophosphamide, immunology, mice

CLC Number: 

  • R286.91

Figure 1

Effect of DBT on tumor growth speed of tumor-bearing mice *P<0.05, vs untreated group; △P<0.05, vs CTX-treated group."

Figure 2

Effect of DBT on survival time of tumor-bearing mice"

Table 1

Effect of DBT on the number of plaque forming cells in splenocytes of tumor-bearing mice ($\bar{x}$±s)"

Group n PFC (×104)
Normal control 10 8.67±0.82*
Untreated 10 4.86±0.89
DBT-treated 10 8.53±1.83*
CTX-treated 10 2.41±0.79*
DBT+CTX-treated 10 5.12±0.93

Table 2

Effect of DBT on the delayed type hypersensitivity response in tumor-bearing mice ($\bar{x}$±s, mm)"

Group n Foot pad swelling
Normal control 10 0.90±0.20*
Untreated 10 0.70±0.10
DBT-treated 10 1.00±0.13*
CTX-treated 10 0.40±0.14*
DBT+CTX-treated 10 0.67±0.17

Table 3

Effect of DBT on the activity of cytotoxic T lymphocytes in tumor-bearing mice ($\bar{x}$±s, %)"

Group n Killing rate
Normal control 10 54.2±6.2*
Untreated 10 35.1±5.9
DBT-treated 10 47.3±7.0*
CTX-treated 10 19.0±7.6*
DBT+CTX-treated 10 32.2±6.4

Table 4

Effect of DBT on the activity of NK cells in the tumor-bearing mice ($\bar{x}$±s, %)"

Group n Killing rate
Normal control 10 84.21±7.91*
Untreated 10 49.78±8.12
DBT-treated 10 72.98±5.74*
CTX-treated 10 29.02±6.71*
DBT+CTX-treated 10 52.65±7.45

Table 5

Effect of DBT on TNF-α production of peritoneal macrophages in tumor-bearing mice ($\bar{x}$±s)"

Group n Absorbance at 570 nm
Normal control 10 0.192±0.021*
Untreated 10 0.313±0.040
DBT-treated 10 0.253±0.025*
CTX-treated 10 0.409±0.037*
DBT+CTX-treated 10 0.291±0.028

Table 6

Effect of DBT on the number of red blood cells and white blood cells in peripheral blood and nucleated cells in bone marrow in tumor-bearing mice ($\bar{x}$±s)"

Group n RBC (1×1012/L) WBC (1×109/L) Nucleated cell (1×105/femur)
Normal control 10 12.11±1.04 10.32±1.71 10.1±1.6
Untreated 10 10.62±1.73 8.93±1.54 9.2±1.3
DBT-treated 10 12.71±1.58 9.32±1.34 10.6±0.9
CTX-treated 10 5.43±0.32* 4.17±0.87* 4.3±0.5*
DBT+CTX-treated 10 9.79±1.2 7.97±1.46 7.9±1.2
[1] Yang XW, Chen YZ, Lin ZX , et al. Anti-lymphoma immunity induced by EL-4 cells transfected with B7. 1 or/and GM-CSF gene. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2002; 18(4):359-362. Chinese with abstract in English.
doi: 10.3321/j.issn:1007-8738.2002.04.016
杨旭伟, 陈元仲, 林振兴 , 等. B7. 1、GM-CSF基因修饰的EL-4细胞激发小鼠抗淋巴瘤免疫反应. 细胞与分子免疫学杂志. 2002; 18(4):359-362.
doi: 10.3321/j.issn:1007-8738.2002.04.016
[2] Sun ZQ. Statistics of medicine. Beijing: People's Medical Publishing House. 2002: 278. Chinese.
孙振球 . 医学统计学.北京: 人民卫生出版社. 2002: 278.
[3] Cunningham AJ, Szenberg A . Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968; 14(4) : 599.
pmid: 5653817
[4] Li AL, Jiang JY, Zhang QY , et al. Cytokines transduced bone marrow stromal cell lines promote immuno- hematopoietic reconstitution in mice after allogeneic bone marrow transplantation. Immunol Lett. 2005 ; 98(2):216-224.
doi: 10.1016/j.imlet.2004.11.018
[5] Xie SS, Lu XF, Qin FH , et al. Primary study on the mechanism of immunoregulatory effect of polysaccharide oi Acanthopanax senticosus ( PAS) . Zhonghua Wei Sheng Wu Xue He Mian Yi Xue Za Zhi. 1989; 9(3):153-155. Chinese.
谢蜀生, 吕秀凤, 秦凤华 , 等. 刺五加多糖免疫调节机理初探.中华微生物学和免疫学杂志. 1989; 9(3):153-155.
[6] Wang Q , Wang B. Examination of the killing activity of NK cells with modified MTT approach. Ningxia Yi Xue Yuan Xue Bao. 1999; 21(2):86-88. Chinese.
王琦, 王波 . 改良的MTT比色法测定NK细胞杀伤活性.宁夏医学院学报. 1999; 21(2):86-88.
[7] Ferrari M, Fornasiero MC, Isetta AM . MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J Immunol methods. 1990; 131(2) : 165-172.
doi: 10.1016/0022-1759(90)90187-Z pmid: 2391427
[8] Xu RR , Yin JL. Effects of compound traditional Chinese drugs for tonification on CFU-GM and CFU-L in leukemia mice. Zhongguo Zhong Yi Yao Ke Ji. 1995; 2(2):23-25 Chinese.
徐瑞荣, 殷建林 . 复方补益中药对白血病小鼠CFU- GM及CFU-L的影响.中国中医药科技. 1995; 2(2):23-25.
[9] Kok LD, Wong CK, Leung KN , et al. Activation of the anti-tumor effector cells hy Radix bupleuri. Immu- nopharmacology. 1995 ; 30(1) : 79-87.
doi: 10.1016/0162-3109(95)00010-Q pmid: 7591716
[10] Roitt IM, Delves PJ. Roitt’s essential immunology. 10th ed. London: Blackwell Publishing Ltd. 2001 : 379.
[11] Wu XQ. Research and development of Chinese drugs for invigoration on anticancer action. Hunan Zhong Yi Yao Dao Bao. 1999; 5(9):10-13. Chinese with abstract in English.
吴雪卿 . 补益中药抗癌作用研究进展.湖南中医药导报. 1999; 5(9):10-13.
[12] Tian WW, Li O, Huang YU , et al. Effect of BuXu HuaYu Prescription on myeloid hematopoiesis in tumorbearing mice after combined chemotherapy. Zhong Yao Xin Yao Yu Lin Chuang Yao Li. 2003; 14(1):3-6. Chinese with abstract in English.
doi: 10.3321/j.issn:1003-9783.2003.01.002
田卫卫, 李偶, 黄映红 , 等. 补虚化瘀方对联合化疗荷瘤小鼠骨髓造血功能的影响.中药新药与临床药理. 2003; 14(1):3-6.
doi: 10.3321/j.issn:1003-9783.2003.01.002
[13] Chen WF. Medical immunology. 4th ed. Beijing: People's Medical Publishing House. 2005: 224. Chinese.
陈慰峰 . 医学免疫学.第4版.北京: 人民卫生出版社. 2005: 224.
[14] HeXH,XiXX. Traditional medicine immunology. Beijing: People's Military Medical Publisher. 2002: 379. Chinese.
贺新怀, 席孝贤 . 中医药免疫学.北京: 人民军医出版社. 2002: 379.
[15] Li XY, Li J. New points of immunopharmacology. Beijing : People’s Medical Publishing House. 2004: 242. Chinese.
李晓玉, 李俊 . 免疫药理学新论.北京: 人民卫生出版社. 2004: 242
[1] Talat Albukhari, Bassem Refaat, El-Sayed Bakr, Sameh Baz, Bodour Rajab, Hossam Gadalla, Mohamed El-Boshy. Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats. Journal of Integrative Medicine, 2022, 20(4): 348-354.
[2] Victor Abou Nehmi Filho, Gilson Masahiro Murata, Ruan Carlos Macêdo de Moraes, Gabriely Cristina Alves Lima, Danielle Araujo de Miranda, Katrin Radloff, Raquel Galvão Figuerêdo Costa, Joyce de Cassia Rosa de Jesus, Jéssica Alves De Freitas, Nayara Izabel Viana, Ruan Pimenta, Katia Ramos Moreira Leite, José Pinhata Otoch, Ana Flávia Marçal Pessoa. A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice. Journal of Integrative Medicine, 2021, 19(5): 439-450.
[3] Hao Gou, Ruo-chen Huang, Yong-hua Su, Wei Li. Design of dual targeting immunomicelles loaded with bufalin and study of their anti-tumor effect on liver cancer. Journal of Integrative Medicine, 2021, 19(5): 408-417.
[4] Yi Ran Hu, San Li Xing, Chuan Chen, Ding Zhu Shen, Jiu Lin Chen. Tiaoxin Recipe, a Chinese herbal formula, inhibits microRNA-34a expression in the APPswe/PS1ΔE9 mouse model of Alzheimer's disease. Journal of Integrative Medicine, 2019, 17(6): 404-409.
[5] Pierre Watcho, Ismaelle Rosine Mpeck, Patrick Brice Deeh Defo, Modeste Wankeu-Nya, Esther Ngadjui, Georges Romeo Bonsou Fozin, Pierre Kamtchouing, Albert Kamanyi. Cyclophosphamide-induced reproductive toxicity: Beneficial effects of Helichrysum odoratissimum (Asteraceae) in male Wistar rats. Journal of Integrative Medicine, 2019, 17(5): 366-373.
[6] Bhesh Raj Sharma, Dong Wook Kim, Dong Young Rhyu. Korean Chungtaejeon tea extract attenuates weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2017, 15(1): 56-63.
[7] Jesmin Mondal, Jayeeta Das, Rajesh Shah, Anisur Rahman Khuda-Bukhsh. A homeopathic nosode, Hepatitis C 30 demonstrates anticancer effect against liver cancer cells in vitro by modulating telomerase and topoisomerase II activities as also by promoting apoptosis via intrinsic mitochondrial pathway. Journal of Integrative Medicine, 2016, 14(3): 209-218.
[8] Qing-qing Zang, Lu Zhang, Ning Gao, Cheng Huang. Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells. Journal of Integrative Medicine, 2016, 14(1): 51-59.
[9] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[10] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[11] Md. Asadujjaman, Ahmed Ullah Mishuk, Md. Aslam Hossain, Utpal Kumar Karmakar. Medicinal potential of Passiflora foetida L. plant extracts: Biological and pharmacological activities. Journal of Integrative Medicine, 2014, 12(2): 121-126.
[12] Marjan Nassiri-Asl, Taghi Naserpour Farivar, Esmail Abbasi, Hamid Reza Sadeghnia, Mehdi Sheikhi, Mina Lotfizadeh, Parisa Bazahang. Effects of rutin on oxidative stress in mice with kainic acid-induced seizure. Journal of Chinese Integrative Medicine, 2013, 11(5): 337-342.
[13] Md. Khirul Islam, Imran Mahmud, Sanjib Saha, Asit Baron Sarker, Himangsu Mondal, A.S.M. Monjur-Al-Hossain, Md. Anisuzzman. Preliminary pharmacological evaluation of Alocasia indica Schott tuber. Journal of Chinese Integrative Medicine, 2013, 11(5): 343-351.
[14] Sanjib Saha, Faroque Hossain, Md. Anisuzzman, Md. Khirul Islam. Pharmacological evaluation of Musa seminifera Lour. Fruit. Journal of Chinese Integrative Medicine, 2013, 11(4): 253-261.
[15] Sunil A. Nirmal, Jayashri M. Ingale, Shashikant R. Pattan, Sanjay B. Bhawar. Amaranthus roxburghianus root extract in combination with piperine as a potential treatment of ulcerative colitis in mice. Journal of Chinese Integrative Medicine, 2013, 11(3): 206-212.
Full text



[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9] Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278