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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (1): 83-88.doi: 10.3736/jcim20080116

• Original Experimental Research • Previous Articles     Next Articles

Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice

Guo-hong Yuan1(), Xiao-jing Pang2, He-chao Ma2   

  1. 1. Department of General Surgery, Third Hospital, Peking University, Beijing 100038, China
    2. Department Basic Medical Sciences, Chengde Medical College, Chengde, Hebei Province 067000, China
  • Received:2007-03-20 Online:2008-01-20 Published:2008-01-15
  • Contact: YUAN Guo-hong E-mail:2003042anbo@sohu.com

Objective: To study the inhibitory effect of Danggui Buxue Tang (DBT), a compound traditional Chinese herbal medicine for supplementing blood, on tumor growth in tumor-bearing mice after inoculation of EL-4 cells, and its immune mechanism as well as its synergic effect in reducing toxicity of cytoxan (CTX).Methods: Experiment was carried out in tumor-bearing mice after inoculation of EL-4 cells. The mice were randomly divided into four groups after 7 days of the inoculation: untreated group, DBT-treated group [24 g/(kg·d)], CTX-treated group [7.5 mg/(kg·d)] and DBT plus CTX-treated group, with another ten normal mice as control. Inhibitory rate of tumor growth, survival time, immune function and variability of blood cells were measured in the mice during the experiment.Results: After treatment of relevant interventions for 15 days, the tumor in the DBT-treated group, CTX-treated group and DBT plus CTX-treated group grew slower than the untreated group (P<0.05). Murine survival time in the DBT-treated group, CTX-treated group and DBT plus CTX-treated group was lengthened as compared with that in the untreated group (P<0.05). Compared with the untreated group, all kinds of immune indexes in the DBT-treated group and DBT plus CTX-treated group were significantly improved (P<0.05), while the immune indexes in the CTX-treated group were decreased (P<0.05). Compared with the CTX-treated group, all kinds of immune indexes in the DBT plus CTX-treated group were significantly improved (P<0.05).Conclusions: DBT can enhance the immune function in tumor-bearing mice and the inhibitory effect of DBT on tumor growth is related to the enhanced immune response. DBT can also increase the therapeutic effects and reduce the side effects of CTX.

Key words: antineoplastic agents, cyclophosphamide, immunology, mice

CLC Number: 

  • R286.91

Figure 1

Effect of DBT on tumor growth speed of tumor-bearing mice *P<0.05, vs untreated group; △P<0.05, vs CTX-treated group."

Figure 2

Effect of DBT on survival time of tumor-bearing mice"

Table 1

Effect of DBT on the number of plaque forming cells in splenocytes of tumor-bearing mice ($\bar{x}$±s)"

Group n PFC (×104)
Normal control 10 8.67±0.82*
Untreated 10 4.86±0.89
DBT-treated 10 8.53±1.83*
CTX-treated 10 2.41±0.79*
DBT+CTX-treated 10 5.12±0.93

Table 2

Effect of DBT on the delayed type hypersensitivity response in tumor-bearing mice ($\bar{x}$±s, mm)"

Group n Foot pad swelling
Normal control 10 0.90±0.20*
Untreated 10 0.70±0.10
DBT-treated 10 1.00±0.13*
CTX-treated 10 0.40±0.14*
DBT+CTX-treated 10 0.67±0.17

Table 3

Effect of DBT on the activity of cytotoxic T lymphocytes in tumor-bearing mice ($\bar{x}$±s, %)"

Group n Killing rate
Normal control 10 54.2±6.2*
Untreated 10 35.1±5.9
DBT-treated 10 47.3±7.0*
CTX-treated 10 19.0±7.6*
DBT+CTX-treated 10 32.2±6.4

Table 4

Effect of DBT on the activity of NK cells in the tumor-bearing mice ($\bar{x}$±s, %)"

Group n Killing rate
Normal control 10 84.21±7.91*
Untreated 10 49.78±8.12
DBT-treated 10 72.98±5.74*
CTX-treated 10 29.02±6.71*
DBT+CTX-treated 10 52.65±7.45

Table 5

Effect of DBT on TNF-α production of peritoneal macrophages in tumor-bearing mice ($\bar{x}$±s)"

Group n Absorbance at 570 nm
Normal control 10 0.192±0.021*
Untreated 10 0.313±0.040
DBT-treated 10 0.253±0.025*
CTX-treated 10 0.409±0.037*
DBT+CTX-treated 10 0.291±0.028

Table 6

Effect of DBT on the number of red blood cells and white blood cells in peripheral blood and nucleated cells in bone marrow in tumor-bearing mice ($\bar{x}$±s)"

Group n RBC (1×1012/L) WBC (1×109/L) Nucleated cell (1×105/femur)
Normal control 10 12.11±1.04 10.32±1.71 10.1±1.6
Untreated 10 10.62±1.73 8.93±1.54 9.2±1.3
DBT-treated 10 12.71±1.58 9.32±1.34 10.6±0.9
CTX-treated 10 5.43±0.32* 4.17±0.87* 4.3±0.5*
DBT+CTX-treated 10 9.79±1.2 7.97±1.46 7.9±1.2
[1] Yang XW, Chen YZ, Lin ZX , et al. Anti-lymphoma immunity induced by EL-4 cells transfected with B7. 1 or/and GM-CSF gene. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2002; 18(4):359-362. Chinese with abstract in English.
doi: 10.3321/j.issn:1007-8738.2002.04.016
杨旭伟, 陈元仲, 林振兴 , 等. B7. 1、GM-CSF基因修饰的EL-4细胞激发小鼠抗淋巴瘤免疫反应. 细胞与分子免疫学杂志. 2002; 18(4):359-362.
doi: 10.3321/j.issn:1007-8738.2002.04.016
[2] Sun ZQ. Statistics of medicine. Beijing: People's Medical Publishing House. 2002: 278. Chinese.
孙振球 . 医学统计学.北京: 人民卫生出版社. 2002: 278.
[3] Cunningham AJ, Szenberg A . Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968; 14(4) : 599.
pmid: 5653817
[4] Li AL, Jiang JY, Zhang QY , et al. Cytokines transduced bone marrow stromal cell lines promote immuno- hematopoietic reconstitution in mice after allogeneic bone marrow transplantation. Immunol Lett. 2005 ; 98(2):216-224.
doi: 10.1016/j.imlet.2004.11.018
[5] Xie SS, Lu XF, Qin FH , et al. Primary study on the mechanism of immunoregulatory effect of polysaccharide oi Acanthopanax senticosus ( PAS) . Zhonghua Wei Sheng Wu Xue He Mian Yi Xue Za Zhi. 1989; 9(3):153-155. Chinese.
谢蜀生, 吕秀凤, 秦凤华 , 等. 刺五加多糖免疫调节机理初探.中华微生物学和免疫学杂志. 1989; 9(3):153-155.
[6] Wang Q , Wang B. Examination of the killing activity of NK cells with modified MTT approach. Ningxia Yi Xue Yuan Xue Bao. 1999; 21(2):86-88. Chinese.
王琦, 王波 . 改良的MTT比色法测定NK细胞杀伤活性.宁夏医学院学报. 1999; 21(2):86-88.
[7] Ferrari M, Fornasiero MC, Isetta AM . MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J Immunol methods. 1990; 131(2) : 165-172.
doi: 10.1016/0022-1759(90)90187-Z pmid: 2391427
[8] Xu RR , Yin JL. Effects of compound traditional Chinese drugs for tonification on CFU-GM and CFU-L in leukemia mice. Zhongguo Zhong Yi Yao Ke Ji. 1995; 2(2):23-25 Chinese.
徐瑞荣, 殷建林 . 复方补益中药对白血病小鼠CFU- GM及CFU-L的影响.中国中医药科技. 1995; 2(2):23-25.
[9] Kok LD, Wong CK, Leung KN , et al. Activation of the anti-tumor effector cells hy Radix bupleuri. Immu- nopharmacology. 1995 ; 30(1) : 79-87.
doi: 10.1016/0162-3109(95)00010-Q pmid: 7591716
[10] Roitt IM, Delves PJ. Roitt’s essential immunology. 10th ed. London: Blackwell Publishing Ltd. 2001 : 379.
[11] Wu XQ. Research and development of Chinese drugs for invigoration on anticancer action. Hunan Zhong Yi Yao Dao Bao. 1999; 5(9):10-13. Chinese with abstract in English.
吴雪卿 . 补益中药抗癌作用研究进展.湖南中医药导报. 1999; 5(9):10-13.
[12] Tian WW, Li O, Huang YU , et al. Effect of BuXu HuaYu Prescription on myeloid hematopoiesis in tumorbearing mice after combined chemotherapy. Zhong Yao Xin Yao Yu Lin Chuang Yao Li. 2003; 14(1):3-6. Chinese with abstract in English.
doi: 10.3321/j.issn:1003-9783.2003.01.002
田卫卫, 李偶, 黄映红 , 等. 补虚化瘀方对联合化疗荷瘤小鼠骨髓造血功能的影响.中药新药与临床药理. 2003; 14(1):3-6.
doi: 10.3321/j.issn:1003-9783.2003.01.002
[13] Chen WF. Medical immunology. 4th ed. Beijing: People's Medical Publishing House. 2005: 224. Chinese.
陈慰峰 . 医学免疫学.第4版.北京: 人民卫生出版社. 2005: 224.
[14] HeXH,XiXX. Traditional medicine immunology. Beijing: People's Military Medical Publisher. 2002: 379. Chinese.
贺新怀, 席孝贤 . 中医药免疫学.北京: 人民军医出版社. 2002: 379.
[15] Li XY, Li J. New points of immunopharmacology. Beijing : People’s Medical Publishing House. 2004: 242. Chinese.
李晓玉, 李俊 . 免疫药理学新论.北京: 人民卫生出版社. 2004: 242
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