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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (1): 83-88.doi: 10.3736/jcim20080116

• Original Experimental Research • Previous Articles     Next Articles

Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice

Guo-hong Yuan1(), Xiao-jing Pang2, He-chao Ma2   

  1. 1. Department of General Surgery, Third Hospital, Peking University, Beijing 100038, China
    2. Department Basic Medical Sciences, Chengde Medical College, Chengde, Hebei Province 067000, China
  • Received:2007-03-20 Online:2008-01-20 Published:2008-01-15
  • Contact: YUAN Guo-hong E-mail:2003042anbo@sohu.com

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Objective: To study the inhibitory effect of Danggui Buxue Tang (DBT), a compound traditional Chinese herbal medicine for supplementing blood, on tumor growth in tumor-bearing mice after inoculation of EL-4 cells, and its immune mechanism as well as its synergic effect in reducing toxicity of cytoxan (CTX).Methods: Experiment was carried out in tumor-bearing mice after inoculation of EL-4 cells. The mice were randomly divided into four groups after 7 days of the inoculation: untreated group, DBT-treated group [24 g/(kg·d)], CTX-treated group [7.5 mg/(kg·d)] and DBT plus CTX-treated group, with another ten normal mice as control. Inhibitory rate of tumor growth, survival time, immune function and variability of blood cells were measured in the mice during the experiment.Results: After treatment of relevant interventions for 15 days, the tumor in the DBT-treated group, CTX-treated group and DBT plus CTX-treated group grew slower than the untreated group (P<0.05). Murine survival time in the DBT-treated group, CTX-treated group and DBT plus CTX-treated group was lengthened as compared with that in the untreated group (P<0.05). Compared with the untreated group, all kinds of immune indexes in the DBT-treated group and DBT plus CTX-treated group were significantly improved (P<0.05), while the immune indexes in the CTX-treated group were decreased (P<0.05). Compared with the CTX-treated group, all kinds of immune indexes in the DBT plus CTX-treated group were significantly improved (P<0.05).Conclusions: DBT can enhance the immune function in tumor-bearing mice and the inhibitory effect of DBT on tumor growth is related to the enhanced immune response. DBT can also increase the therapeutic effects and reduce the side effects of CTX.

Key words: antineoplastic agents, cyclophosphamide, immunology, mice

CLC Number: 

  • R286.91

Figure 1

Effect of DBT on tumor growth speed of tumor-bearing mice *P<0.05, vs untreated group; △P<0.05, vs CTX-treated group."

Figure 2

Effect of DBT on survival time of tumor-bearing mice"

Table 1

Effect of DBT on the number of plaque forming cells in splenocytes of tumor-bearing mice ($\bar{x}$±s)"

Group n PFC (×104)
Normal control 10 8.67±0.82*
Untreated 10 4.86±0.89
DBT-treated 10 8.53±1.83*
CTX-treated 10 2.41±0.79*
DBT+CTX-treated 10 5.12±0.93△

Table 2

Effect of DBT on the delayed type hypersensitivity response in tumor-bearing mice ($\bar{x}$±s, mm)"

Group n Foot pad swelling
Normal control 10 0.90±0.20*
Untreated 10 0.70±0.10
DBT-treated 10 1.00±0.13*
CTX-treated 10 0.40±0.14*
DBT+CTX-treated 10 0.67±0.17△

Table 3

Effect of DBT on the activity of cytotoxic T lymphocytes in tumor-bearing mice ($\bar{x}$±s, %)"

Group n Killing rate
Normal control 10 54.2±6.2*
Untreated 10 35.1±5.9
DBT-treated 10 47.3±7.0*
CTX-treated 10 19.0±7.6*
DBT+CTX-treated 10 32.2±6.4△

Table 4

Effect of DBT on the activity of NK cells in the tumor-bearing mice ($\bar{x}$±s, %)"

Group n Killing rate
Normal control 10 84.21±7.91*
Untreated 10 49.78±8.12
DBT-treated 10 72.98±5.74*
CTX-treated 10 29.02±6.71*
DBT+CTX-treated 10 52.65±7.45△

Table 5

Effect of DBT on TNF-α production of peritoneal macrophages in tumor-bearing mice ($\bar{x}$±s)"

Group n Absorbance at 570 nm
Normal control 10 0.192±0.021*
Untreated 10 0.313±0.040
DBT-treated 10 0.253±0.025*
CTX-treated 10 0.409±0.037*
DBT+CTX-treated 10 0.291±0.028△

Table 6

Effect of DBT on the number of red blood cells and white blood cells in peripheral blood and nucleated cells in bone marrow in tumor-bearing mice ($\bar{x}$±s)"

Group n RBC (1×1012/L) WBC (1×109/L) Nucleated cell (1×105/femur)
Normal control 10 12.11±1.04 10.32±1.71 10.1±1.6
Untreated 10 10.62±1.73 8.93±1.54 9.2±1.3
DBT-treated 10 12.71±1.58 9.32±1.34 10.6±0.9
CTX-treated 10 5.43±0.32* 4.17±0.87* 4.3±0.5*
DBT+CTX-treated 10 9.79±1.2△ 7.97±1.46△ 7.9±1.2△
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