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Journal of Chinese Integrative Medicine ›› 2003, Vol. 1 ›› Issue (2): 119-121.doi: 10.3736/jcim20030210

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Effect and mechanism of Tangshenning Recipe on micro-albuminuria in rats with early diabetic nephropathy

Li-qun He(), He-xin Cao, Ya-jing Shen   

  1. Research Center of Pharmacology and Toxicology of New Chinese Drugs, Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China
  • Received:2003-03-07 Online:2003-06-20 Published:2018-10-19

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Objective

To study the modifying effect and mechanism of Tangshenning Recipe on micro-albuminuria in rats with early diabetic nephropathy (DN).


Methods

Male Wistar rats were randomly divided into the normal group (n=8)and model group (n=24). Intraperitoneal injecting of streptozotocin (STZ) plus complete Freund's adjuvant (CFA) was applied once a week for 3 times to induce the DN rats model. Three weeks later, the model group rats were randomly divided into pathologic group (n=8), monopril group (n=8) and Tangshenning Recipe group (n=8) according to the 24 h U-Alb. Each group's renal hemodynamics index and SOD, GSH, MDA in renal tissue were determined by radioimmunoassay (RIA) and colorimetric method respectively.


Results

The levels of plasmaticTXB2, the ratio of TXB2 and 6-keto-PGF1α, and the CGRP in pathologic group were significantly higher than those in normal group. The levels of plasmatic ET decreased obviously, SOD decreased and MDA increased significantly in the rats' renal tissue of pathologic group. The levels of plasmatic TXB2, the ratio of TXB2 and 6-keto-PGF1α decreased significantly in both Tangshenning Recipe group and monopril group, and the therapeutic effect of Tangshenning Recipe group was better than that of monopril group. SOD was higher and MDA was lower in Tangshenning Recipe group than that in pathologic group.


Conclusion

The results indicates that Tangshenning Recipe can lower the micro-albuminuria in early DN rats, the mechanism of which probably lies in the modification of glycometabolism, the ratio of TXB2 and 6-keto-PGF1α, the plasmatic CGRP and the renal lipid preoxidation.

Key words: Shenqi Recipe, glomerular mesangial cells, interstitial fibroblasts

CLC Number: 

  • R587.24

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组别 n 血糖(mmol/L) U-Alb(μg/24 h) ET(ng/L) CGRP(ng/L)
正常组 8 6.21±1.38 6.32±0.68 100.23±44.70 8.89±1.23
模型组 7 26.89±1.79* 21.63±20.76* 45.14±14.36* 12.80±1.40*
糖肾宁组 7 20.39±7.39*△ 9.77±3.72△ 57.62±35.09* 9.26±2.33△
蒙诺组 7 23.73±6.88* 10.45±5.18△ 28.72±13.02* 11.06±2.68

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组别 n TXB2(ng/L) 6-keto-PGF1α(ng/L) TXB2/6-keto-PGF1α
正常组 8 50.74±26.97 6.93±2.12 8.39±5.55
模型组 6 139.42±18.99* 7.02±1.63 20.49±4.19*
糖肾宁组 6 88.01±33.64△ 7.57±2.88 9.91±4.46△
蒙诺组 6 93.78±40.16*△ 5.63±2.12 14.41±6.76

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组别 n SOD（μU/L） MDA（pmol/L）
正常组 8 362.54±25.03 18.50±5.71
模型组 7 282.75±24.10* 33.77±9.66*
糖肾宁组 7 321.63±24.10*△ 23.31±8.07△
蒙诺组 7 303.07±15.66* 29.70±10.34*
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