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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 536-540.doi: 10.3736/jcim20070512

• Original Experimental Research • Previous Articles     Next Articles

Effects of Astragalus on expression of renal angiopoietin receptor Tie-2 in diabetic rats

Hong-wei Gu1, Zhao-hui Ni1, Le-yi Gu1, Yu-cheng Yan1, Hui-li Dai1, Ning-li Li2, Min-fang Zhang1, Jia-qi Qiang1   

  1. 1. Department of Nephrology,Renji Hospital,Medical College of Shanghai Jiaotong University,Shanghai 200127,China
    2. Institute of Immunology,Medical College of Shanghai Jiaotong University,Shanghai 200025,China
  • Online:2007-09-20 Published:2007-09-15

Objective: To study the expression of angiopoietin receptor Tie-2 in the renal tissue of diabetic rats and the effects of Astragalus.Methods: SD rats were randomly divided into normal control group, diabetes group and Astragalus-treated group. The expression of receptor Tie-2 in the renal tissue was assessed by using real-time quantitative polymerase chain reaction and immunohistochemical method.Results: Glomerule Tie-2 protein expression was significantly elevated in the diabetes group as compared with the normal control group (P<0.01). Glomerule Tie-2 protein expression in the Astragalus-treated group was decreased as compared with the diabetes group (P<0.01).Conclusion: Tie-2 may play an important role in the pathogenesis of the early stage diabetic renal injury. The reno-protection effect of Astragalus may be mediated by down-regulating the expression of Tie-2 in the kidney tissue of diabetic rats.

Key words: diabetic nephropathies, angiogenesis factor, Astragalus, rats

CLC Number: 

  • R587.24

Table 1

Sequences of primers for real-time PCR amplified genes"

mRNA Sequence Forward primer Reverse primer Length

Table 2

Metabolic and physiological parameters in three groups (ヌ±S)"

Group n BG (mmol/L) BW (g) KWI (mg/g) SBP (mmHg) Upro/Cr (μg/μmol)
Normal control 6 12.68±1.14 467.64±6.32 0.30±0.05 114.74±8.24 61.58±7.51
Diabetes 6 37.07±10.67** 254.50±60.45** 0.59±0.07** 139.50±6.08** 169.21±19.19**
Astragalus-treated 6 45.56±10.15** 221.67±67.78** 0.68±0.13** 143.17±17.31** 117.90±44.85**△△

Table 3

Comparisons of VG and RM among three groups (ヌ±S)"

Group n VG(×106, μm3) RM(%)
Normal control 6 18.77±3.89 37.78±3.97
Diabetes 6 31.47±8.00** 52.30±9.44**
Astragalus-treated 6 29.37±3.62** 54.62±8.13**

Table 4

Expression of renal angiopoietin receptor Tie-2 in three groups (ヌ±S)"

Group n Tie-2 mRNA Tie-2 protein (%)
Normal control 6 132.13±63.12 26.55±3.94
Diabetes 6 156.31±33.34 43.04±9.45**
Astragalus-treated 6 117.72±47.92 28.02±6.47△△

Figure 1

Renal Tie-2 expression in 3 groups (Immunohistochemical method, ×400) A: Normal control group; B: Diabetes group; C: Astragalus-treated group."

[1] Nyengaard JR, Rasch R . The impact of experimental diabetes mellitus in rats on glomerular capillary number and sizes[J]. Diabetologia, 1993,36(3):189-194
doi: 10.1007/BF00399948 pmid: 8462766
[2] Yamamoto Y, Maeshima Y, Kitayama H , et al. Tumstatin peptide, an inhibitor of angiogenesis, prevents glomerular hypertrophy in the early stage of diabetic nephropathy[J]. Diabetes, 2004,53(7):1831-1840
[3] Yancopoulos GD, Davis S, Gale NW , et al. Vascular-specific growth factors and blood vessel formation[J]. Nature, 2000,407(6801):242-248
doi: 10.1038/35025215 pmid: 11001067
[4] Kang DH, Kanellis J, Hugo C , et al. Role of the microvascular endothelium in progressive renal disease[J]. J Am Soc Nephrol, 2002,13(3):806-816
doi: 10.1089/089277902753619654 pmid: 11856789
[5] Lee GT, Ha H, Jung M , et al. Delayed treatment with lithospermate B attenuates experimental diabetic renal injury[J]. J Am Soc Nephrol, 2003,14(3):709-720
[6] 郭志新, 邱明才 . 洛沙坦对大鼠糖尿病模型肾脏转化生长因子βⅠ型、Ⅱ型受体表达的影响[J]. 中华内科杂志, 2003,42(6):403-408
Guo ZX, Qiu MC . Losartan downregulates the expression of transforming growth factor-β type Ⅰ and type Ⅱ receptors in kidney of diabetic rat[J]. Zhonghua Nei Ke Za Zhi, 2003,42(6):403-408
[7] Kang DH, Anderson S, Kim YG , et al. Impaired angiogenesis in the aging kidney: vascular endothelial growth factor and thrombospondin-1 in renal disease[J]. Am J Kidney Dis, 2001,37(3):601-611
doi: 10.1016/S0272-6386(01)80019-6
[8] 高艳丽, 谌贻璞, 董鸿瑞 , 等. 慢性马兜铃酸肾病肾间质纤维化发病机制的初步探讨[J]. 中华肾脏病杂志, 2005,21(1):31-35
Gao YL, Chen YP, Dong HR , et al. Experimental study on renal interstitial fibrosis of chronic aristolochic acid nephropathy in rats[J]. Zhonghua Shen Zang Bing Za Zhi, 2005,21(1):31-35
[9] Sato TN, Tozawa Y, Deutsch U , et al. Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation[J]. Nature, 1995,376(6535):70-74
doi: 10.1038/376070a0 pmid: 7596437
[10] Dumont DJ, Gradwohl G, Fong GH , et al. Dominant-negative and targeted null mutations in the endothelial receptor tyrosine kinase, tek, reveal a critical role in vasculogenesis of the embryo[J]. Genes Dev, 1994,8(16):1897-1909
[11] Isner JM, Asahara T . Angiogenesis and vasculogenesis as therapeutic strategies for postnatal neovascularization[J]. J Clin Invest, 1999,103(9):1231-1236
[12] Patel JI, Hykin PG, Gregor ZJ , et al. Angiopoietin concentrations in diabetic retinopathy[J]. Br J Ophthalmol, 2005,89(4):480-483
doi: 10.1136/bjo.2004.049940 pmid: 1772595
[13] 吴文清, 李英, 赵金彩 , 等. 野黄芪甙元对实验性糖尿病大鼠肾脏的保护作用[J]. 中国中西医结合肾病杂志, 2001,2(8):444-446
Wu WQ, Li Y, Zhao JC , et al. Renal protective effect of erigeron breviscapus in diabetic rats[J]. Zhongguo Zhong Xi Yi Jie He Shen Bing Za Zhi, 2001,2(8):444-446
[14] Cai Q, Li X, Wang H . Astragali and angelica protect the kidney against ischemia and reperfusion injury and accelerate recovery[J]. Chin Med J (Engl), 2001,114(2):119-123
[15] 周筱静, 沈建雄, 黄岚 . 益肾固精方改善早期糖尿病肾病大鼠蛋白尿机制探讨[J]. 中西医结合学报, 2003,1(1):39-41
Zhou XJ, Shen JX, Huang L . Study of the mechanism of preventing proteinuria by Yishen Gujing Decoction(YSGJD) in early diabetic nephropathy[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(1):39-41
[16] 徐郁杰, 张庆怡, 陆敏 , 等. 黄芪对糖尿病大鼠肾皮质TGF-β表达的影响[J]. 中华内分泌代谢杂志, 1998,14(5):312-314
Xu YJ, Zhang QY, Lu M , et al. Effect of Astragalus membranaceus on expression of TGF-β in renal cortex of diabetic rats[J]. Zhonghua Nei Fen Mi Dai Xie Za Zhi, 1998,14(5):312-314
[17] 牟姗, 张庆怡, 倪兆慧 , 等. 黄芪对高糖作用下肾间质成纤维细胞表达HGF的影响[J]. 中国中西医结合肾病杂志, 2002,3(1):7-9
Mou S, Zhang QY, Ni ZH , et al. Effect of Astragalus membranaceus on HGF by renal interstitial fibroblasts in the high glucose[J]. Zhongguo Zhong Xi Yi Jie He Shen Bing Za Zhi, 2002,3(1):7-9
[18] 杨蓉, 倪兆慧, 钱家麒 , 等. 人类转化生长因子β诱导基因-克隆3在糖尿病大鼠肾组织中的表达以及洛沙坦和黄芪对其影响[J]. 中华肾脏病杂志, 2004,20(5):347-350
Yang R, Ni ZH, Qian JQ , et al. The significance of βig-h3 expression in renal tissue of diabetic rats and the effects of losartan and Astragalus memberaneous[J]. Zhonghua Shen Zang Bing Za Zhi, 2005,20(5):347-350
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