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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (1): 39-41.doi: 10.3736/jcim20040115

• Original Experimental Research • Previous Articles     Next Articles

Effect of tetromethylpyrazine and aminoguanidine on expression of vascular endothelial growth factor in kidney of diabetic rats

Yan Huang, Shao-qiang Chen, Geng Zhang, Rui-hua Chen   

  1. Department of Anatomy & Histology,Fujian Medical University,Fuzhou,Fujian Province 350004,China
  • Online:2004-01-20 Published:2018-10-25

Objective: To investigate the therapeutic mechanism of tetromethylpyrazine and aminoguanidine on diabetic nephropathy. Methods: Diabetic rats were induced by streptozotocin. They were divided into 5 groups: normal control group (group C), untreated diabetic group (group DM), tetromethylpyrazine treated group (group TMP), aminoguanidine treated group (group AG) and tetromethylpyrazine and aminoguanidine treated group (group TMP+AG). The expression of vascular endothelial growth factor (VEGF) in renal cortex of the rats in each group was observed by immunohistochemical staining after 12 weeks. Results: The expression of VEGF in renal cortex of the rats in group TMP+AG and group C was alike. The expression of VEGF in renal cortex of group TMP and group AG decreased significantly as compared with that of group C, but was still above normal level.Conclusion: The therapeutic mechanism of tetromethylpyrazine and aminoguanidine on diabetic nephropathy may be inhibiting the over-expression of VEGF in kidney of diabetic rats.

Key words: tetromethylpyrazine, aminoguanidine, diabetic nephropathy, vascular endothelial growth factor

CLC Number: 

  • R587.24

Tab 1

Comparison of weight and blood sugar of 5 groups (ヌ±S)"

Group n Weight (g) Blood sugar(mmol/L)
4th week 8th week 12th week 4th week 8th week 12th week
Group C 10 281±15 301±12 316±11 3.9±1.1 4.2 ±1.5 4.3±0.8
Group DM 10 181±8** 169±11** 158±6** 21.4±2.3** 22.9±1.3** 22.8±1.9**
GroupTMP 10 203±7**△△ 198±9**△△ 193±9**△△ 19.1±1.8**△△ 17.3±1.5**△△ 20.3±2.7**△△
Group AG 10 212±15**△△ 206±17**△△ 213±7**△△ 16.7±2.1**△△ 15.4±1.5**△△ 13.4±1.9**△△
Group TMP+AG 10 205±14**△△ 214±13**△△ 219±9**△△ 12.3±1.7**△△ 10.4±1.2**△△ 5.4±1.5**△△

Tab 2

Comparison of HbA1c, 24 h urine protein, Ccr and kidney weight/body weight of 5 groups (ヌ±S)"

Group n HbA1c(%) 24 h urinary protein
(mg·24h-1)
Ccr(ml·min-1·kg-1) Kidney weight/Body weight
(×10-3)
Group C 10 0.760±0.540 4.25±0.57 3.25±0.41 6.89±0.53
Group DM 10 3.932±1.044** 28.68±1.89** 6.67±1.56** 15.28±1.47**
Group TMP 10 1.745±1.170**△△ 15.99±1.34**△△ 5.49±0.67**△△ 10.46±0.84**△△
Group AG 10 1.840±0.941**△△ 13.15±0.76**△△ 4.82±0.71**△△ 9.33±1.05**△△
Group TMP+AG 10 0.856±0.832**△△ 10.21±1.22**△△ 4.43±0.58**△△ 8.25±0.46**△△

Fig.1

Immunohistochemistry of VEGF in renalcortex of the rat in group C(×400)"

Fig.2

Immunohistochemistry of VEGF in renalcortex of the rat in group DM(×400)"

Fig.3

Immunohistochemistry of VEGF in renalcortex of the rat in group TMP+AG(×400)"

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