Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2003, Vol. 1 ›› Issue (2): 122-124.doi: 10.3736/jcim20030211

Previous Articles     Next Articles

Influence of Shenqi Recipe on mesangial cells and interstitial fibroblasts of chronic renal failure rats

Jing Mu, Yi-kui Li, Sheng-guang Fu   

  1. Research Center of Pharmacology and Toxicology of New Chinese Drugs, Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China
  • Received:2003-03-30 Online:2003-06-20 Published:2018-10-19


To observe the effect of Shenqi Recipe on the proliferation of glomerular mesangial cells (MC) and interstitial fibroblasts (FC) in rats.


The effect of serum containing prepared drugs from normal and chronic renal failure rats treated by Shenqi Recipe on glomerular mesangial cells and intersititial fibroblasts in vitro was observed by MTT colorimetric assay and serophrmacology.


Compared with the blank sample normal rats, serum containing Shenqi Recipe inhibited the proliferation of MC and FC (P<0.001), and the serum containing Shenqi Recipe of CRF rats had significantly inhibited the proliferation of MC and FC compared with the model group (P<0.001).


Shenqi Recipe can postpone CRF progress by this mechanism.

Key words: Shenqi Recipe, glomerular mesangial cells, interstitial fibroblasts

CLC Number: 

  • R285.5


血清添加浓度 胎牛血清
20% 0.226±0.01 0.214±0.01 0.172±0.01**
40% 0.294±0.03 0.172±0.04**


血清添加浓度 胎牛血清
10% 1.41±0.05 1.47±0.08 1.05±0.12***
20% 1.44±0.06 1.23±0.02***
40% 1.35±0.08 0.79±0.12***


组别 添加浓度 OD值
假手术组 20% 1.03±0.06**
模型组 20% 1.28±0.25
参芪组 20% 0.81±0.24**
洛汀新组 20% 0.82±0.07**


组别 添加浓度 OD值
假手术组 10% 1.40±0.06*
模型组 10% 1.48±0.08
参芪组 10% 1.18±0.14**
洛汀新组 10% 1.26±0.11**
[1] 沈壮雷, 李乃英 . 保元大黄汤治疗慢性肾功能衰竭的临床研究[J]. 中国中西医结合杂志, 1994,14(5):268-270
doi: 10.7661/CJIM.1994.5.268
[2] 于文慧, 王广有, 马腾骧 , 等. 用改良大部分肾切除法制作的慢性肾衰动物模型[J]. 中华实验外科杂志, 1995,12(4):235-237
[3] 李仪奎 . 中药血清药理学实验方法的若干研究[J]. 中药新药与临床药理, 1999,10(2):95-98
[4] 谌贻璞 . 肾小球系膜细胞培养[J]. 北京医科大学学报, 1988,20(4):335-336
[5] 潘晓勤, 王晓燕 . 肾小球系膜细胞培养及鉴定[J]. 南京医科大学学报, 1995,15(1):222-223
[6] Lonnemann G, Shapiro L, Engler-Blum G , et al. Cytokines in human renal interstitial fibrosis.I.Intrinsic interleukin-1 is a paracrine growth factor for cultured fibrosis-derived kidney fibroblasts[J]. Kidney Int, 1995,47(3):837-844
doi: 10.1038/ki.1995.126
[7] 王伟铭, 姚建 . 人肾间质细胞培养的研究[J]. 上海第二医科大学学报, 1998,18(6):455-457
[8] 陈广平, 郭慕依 . 血小板衍生性生长因子对体外培养人肾小球系膜细胞生长的影响[J]. 中华病理学杂志, 1997,26(4):200-202
[9] 王海燕 . 重视和研究小管-间质损害在肾小球疾病发展和预后中的作用[J]. 中华肾脏病杂志, 2000,16(1):5-6
doi: 10.3760/j.issn:1001-7097.2000.01.001
[1] Li-qun He, He-xin Cao, Ya-jing Shen. Effect and mechanism of Tangshenning Recipe on micro-albuminuria in rats with early diabetic nephropathy. Journal of Chinese Integrative Medicine, 2003, 1(2): 119-121.
Full text



[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9] Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278