Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2003, Vol. 1 ›› Issue (2): 119-121.doi: 10.3736/jcim20030210

Previous Articles     Next Articles

Effect and mechanism of Tangshenning Recipe on micro-albuminuria in rats with early diabetic nephropathy

Li-qun He(), He-xin Cao, Ya-jing Shen   

  1. Research Center of Pharmacology and Toxicology of New Chinese Drugs, Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China
  • Received:2003-03-07 Online:2003-06-20 Published:2018-10-19


To study the modifying effect and mechanism of Tangshenning Recipe on micro-albuminuria in rats with early diabetic nephropathy (DN).


Male Wistar rats were randomly divided into the normal group (n=8)and model group (n=24). Intraperitoneal injecting of streptozotocin (STZ) plus complete Freund's adjuvant (CFA) was applied once a week for 3 times to induce the DN rats model. Three weeks later, the model group rats were randomly divided into pathologic group (n=8), monopril group (n=8) and Tangshenning Recipe group (n=8) according to the 24 h U-Alb. Each group's renal hemodynamics index and SOD, GSH, MDA in renal tissue were determined by radioimmunoassay (RIA) and colorimetric method respectively.


The levels of plasmaticTXB2, the ratio of TXB2 and 6-keto-PGF1α, and the CGRP in pathologic group were significantly higher than those in normal group. The levels of plasmatic ET decreased obviously, SOD decreased and MDA increased significantly in the rats' renal tissue of pathologic group. The levels of plasmatic TXB2, the ratio of TXB2 and 6-keto-PGF decreased significantly in both Tangshenning Recipe group and monopril group, and the therapeutic effect of Tangshenning Recipe group was better than that of monopril group. SOD was higher and MDA was lower in Tangshenning Recipe group than that in pathologic group.


The results indicates that Tangshenning Recipe can lower the micro-albuminuria in early DN rats, the mechanism of which probably lies in the modification of glycometabolism, the ratio of TXB2 and 6-keto-PGF1α, the plasmatic CGRP and the renal lipid preoxidation.

Key words: Shenqi Recipe, glomerular mesangial cells, interstitial fibroblasts

CLC Number: 

  • R587.24


组别 n 血糖(mmol/L) U-Alb(μg/24 h) ET(ng/L) CGRP(ng/L)
正常组 8 6.21±1.38 6.32±0.68 100.23±44.70 8.89±1.23
模型组 7 26.89±1.79* 21.63±20.76* 45.14±14.36* 12.80±1.40*
糖肾宁组 7 20.39±7.39* 9.77±3.72 57.62±35.09* 9.26±2.33
蒙诺组 7 23.73±6.88* 10.45±5.18 28.72±13.02* 11.06±2.68


组别 n TXB2(ng/L) 6-keto-PGF1α(ng/L) TXB2/6-keto-PGF1α
正常组 8 50.74±26.97 6.93±2.12 8.39±5.55
模型组 6 139.42±18.99* 7.02±1.63 20.49±4.19*
糖肾宁组 6 88.01±33.64 7.57±2.88 9.91±4.46
蒙诺组 6 93.78±40.16* 5.63±2.12 14.41±6.76


组别 n SOD(μU/L) MDA(pmol/L)
正常组 8 362.54±25.03 18.50±5.71
模型组 7 282.75±24.10* 33.77±9.66*
糖肾宁组 7 321.63±24.10* 23.31±8.07
蒙诺组 7 303.07±15.66* 29.70±10.34*
[1] 于德民, 吴锐, 尹潍 , 等. 实验性链脲佐菌素糖尿病动物模型的研究[J]. 中国糖尿病杂志, 1995,3(2):105-109
[2] Cohen MP, Surma ML . Effect of diabetes on in vivo metabolism of S-labeled glomerular basement membrane[J]. Diabetes, 1984,33(1):8-12
doi: 10.2337/diab.33.1.8
[3] 李建生, 郑士荣 . 糖肾安对糖尿病肾脏高滤过因素影响的实验研究[J]. 辽宁中医杂志, 1998,25(1):43-45
[4] 杨俊伟, 黎磊石, 张真 , 等. 大黄治疗糖尿病肾病的实验研究[J]. 中华内分泌代谢杂志, 1993,9(4):222-224
[1] Jing Mu, Yi-kui Li, Sheng-guang Fu. Influence of Shenqi Recipe on mesangial cells and interstitial fibroblasts of chronic renal failure rats. Journal of Chinese Integrative Medicine, 2003, 1(2): 122-124.
Full text



[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228