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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (2): 106-110.doi: 10.3736/jcim20040209

• Original Experimental Research • Previous Articles     Next Articles

Effects of fraction from Phyllanthus urinaria on thrombosis and coagulation system in animals

Zhi-qiang Shen1, Peng Chen2, Li Duan3, Zhe-jun Dong4, Zhi-he Chen1, Ji-kai Liu4   

  1. 1.Yunnan Pharmacological Laboratory of Natural Products, Kunming Medical College, Kunming, Yunnan Province 650031, China
    2.Kunming Shenghuo Pharmaceutical Ltd Co, Kunming, Yunnan Province 650217, China
    3.Department of Radiology, First AffiliatedHospital of Kunming Medical College, Kunming, Yunnan Province 650031, China
    4.Kunming Institute of Botany, Chinese Academyof Sciences, Kunming, Yunnan Province 650204, China
  • Online:2004-03-20 Published:2018-10-25

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Objective: To investigate the effects of PUW (a fraction containing 60% corilagin from a Chinese herbal plant Phyllanthus urinaria ) on thrombosis and coagulation system. Methods: Myers's method was used to evaluate the protection of intravenously administered PUW against the male mouse sudden death caused by injection of 75 mg/kg arachidonic acid in the tail vein; Charlton's method was modified to observe antithrombotic effect of PUW in rat electrically stimulated carotid artery thrombosis model; and the model of venous thrombosis was produced to investigate the antithrombotic effect of PUW. Rosette assay was used to observe the effect of PUW on platelet-neutrophil adhesion. The effects of PUW were monitored on euglobulin lysis time (ELT), prothrombin time (PT), kaolin partial thromboplastin time (KPTT), and bleeding time (BT) in rats, according to the methods of Kowalski, HUANG Zheng-Liang, and GU Yue-Fang, et al, respectively. Results: The results showed that PUW administered intravenously significantly decreased the mouse mortality, prolonged the occlusion time of rat carotid arteries, and reduced the wet and dry thrombus weight of the inferior vena cava, respectively. PUW markedly inhibited the binding of activated platelets to neutrophils, obtaining 39.7 mg/L of the medium inhibitory concentration. Intravenously administered PUW significantly shortened ELT, prolonged KPTT while had no influence on PT; PUW increased BT in rat tail tips but the BT caused by PUW was much shorter than that by aspirin or urokinase. Conclusion: It is suggested that PUW has the potential of antithrombosis due to its inhibition of platelet-neutrophil adhesion. PUW shows the tendency to bleeding, however, it could not cause serious bleeding side effect as compared with aspirin or urokinase.

Key words: Phyllanthus urinaria, thrombosis, platelets, neutrophils, coagulation system

CLC Number: 

  • R285.5

Tab 1

Protective effect of intravenously administeredPUW against mouse sudden death induced by injection of 75 mg/kg arachidonic acid in tail vein"

Group Died/Total (Cases) Mortality（%）
Saline 12/15 80
PUW（2.5 mg/kg） 6/15 40*
PUW（5 mg/kg） 3/15 20*
PUW（10 mg/kg） 3/15 20*
Aspirin（5 mg/kg） 4/15 26.7*

Tab 2

Effect of intravenously administered PUW on electrically stimulated carotid arterial thrombosis in rats (ヌ±S)"

Group n Occlusion time（min）
Saline 8 8.3±1.4
PUW（2.5 mg/kg） 8 27.2±1.7**
PUW（5 mg/kg） 8 31.2±2.1**
PUW（10 mg/kg） 8 36.7±1.9**△
Aspirin（5 mg/kg） 8 27.8±1.6**

Tab 3

Effect of intravenously administered PUW on rat inferior vena cava thrombosis (ヌ±S)"

Group n Thrombus weight (mg)
Wet weight Inhibition (%) Dried weight Inhibition (%)
Saline 8 10.3±1.8 3.8±0.7
PUW（2.5 mg/kg） 8 6.0±1.1* 39.4 2.8±0.6* 31.7
PUW（5 mg/kg） 8 5.2±0.9** 44.4 2.2±0.7** 46.3
PUW（10 mg/kg） 8 3.7±1.0**△ 62.6 1.7±0.6**△ 58.5
Aspirin（5 mg/kg） 8 6.1±1.3** 38.4 2.9±0.5** 29.3

Tab 4

Effect of intravenously administered 10 mg/kg PUW on ELT in rabbits (ヌ±S)"

Group n ELT (min)
0 min 10 min 30 min 60 min 120 min

Tab 5

Effect of intravenously administered 10 mg/kg PUW on PT in rabbits (ヌ±S)"

Group n PT (s)
0 min 10 min 30 min 60 min 120 min
Saline 6 13.7±2.5 12.8±1.6 14.3±2.4 11.6±2.7 12.4±2.7
Urokinase (20 000 IU/kg) 6 12.4±2.1 13.8±1.9 14.6±2.3 13.8±3.1 13.6±1.3
PUW (10 mg/kg) 6 13.4±2.2 13.6±2.6 13.1±1.6 12.4±2.7 13.4±3.6

Tab 6

Effect of intravenously administered 10 mg/kg PUW on KPTT in rabbits (ヌ±S)"

Group n KPTT (s)
0 min 10 min 30 min 60 min 120 min
Saline 6 33.8±3.5 37.6±4.3 36.1±3.7 34.2±4.1 41.5±5.4
Urokinase(20 000 IU/kg) 6 32.8±3.4 35.5±6.2 41.8±3.1 38.6±4.3 34.7±2.8
PUW(10 mg/kg) 6 35.7±4.4 43.1±5.4 50.1±7.8** 85.1±10.9** 57.5±9.4**

Tab 7

Effect of intravenously administered PUW on bleeding time of rat tail tip (ヌ±S)"

Group n Bleeding time (min)
Saline 8 16.4±3.1
PUW (10 mg/kg) 8 19.8±3.6*Δ
Urokinase (20 000 IU/kg) 8 30.2±4.2**
Aspirin (5 mg/kg) 8 26.1±3.8**
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