[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (1): 31-34.doi: 10.3736/jcim20050110

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shenmai Huoxue Decoction on early diabetic peripheral neuropathy in rats

Hong-li Xue,Wen-jian Wang,Jian-qiu Chen   

  1. Institute of Chinese Integrative Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2004-05-18 Online:2005-01-20 Published:2005-01-20
  • Contact: Wen-jian Wang E-mail:wj6518@fudan.edu.cn

RichHTML

1

PDF (PC)

73

Like

Objective

To evaluate the effects of Shenmai Huoxue Decoction (SMHXD) on early diabetic peripheral neuropathy (DPN) in rats.

Methods

The rats were randomly divided into 3 groups: normal group, diabetic model group and SMHXD-treated diabetic group. The diabetic rats were induced by streptozotocin (STZ) intraperitoneally. After the treatment, sensory nerve conduction velocity (SNCV) of caudal nerve was detected with evoked electromyography, and the activity of aldose reductase (AR) in erythrocytes and the concentration of endothelin (ET) in plasma were measured.

Results

It was showed that the SNCV reduced significantly and the activity of AR in erythrocytes and the concentration of ET in plasma were increased significantly in diabetic group as compared with those in the normal group. The SNCV was increased, and the activity of AR in erythrocytes and the concentration of ET in plasma were improved significantly in SMHXD-treated diabetic group after the treatment.

Conclusion

The results indicate that SMHXD can alleviate the lesion of DPN in the early stage of diabetic rats.

Key words: Shenmai Huoxue Decoction, Diabetic neuropathy, Animals, Laboratory

CLC Number: 

  • R289.5

Tab 1

Effects of SMHXD on weight and blood glucose concentration of experimental rats ($\bar{x}$±s)"

Group n Weight
(g)		 Blood glucose
(mmol/L)
Normal group 11 323.82±29.30 4.01±0.56
Diabetic group 11 268.82±20.56* 31.13±3.05*
SMHXD-treated group 12 261.67±17.38* 26.97±5.44*

Tab 2

Effects of SMHXD on nerve conduction velocity of experimental rats ($\bar{x}$±s)"

Group n SNCV (m/s) Latency (ms) Amplitude (mV)
Normal group 11 34.85±3.00△ 0.89±0.12△ 41.05±10.83△
Diabetic group 10 23.46±5.45 1.28±0.23 12.39±5.80
SMHXD-treated group 12 29.47±3.68△ 1.00±0.15△ 36.19±7.05△

Tab 3

Effects of SMHXD on activity of AR in erythrocytes and concentration of ET in plasma in experimental rats ($\bar{x}$±s)"

Group AR （U/g Hb） ET （ng/L）
Normal group 0.76±0.14△（n=9） 233.20±25.46△（n=10）
Diabetic group 1.42±0.44 （n=10） 304.54±47.15 （n=10）
SMHXD treated group 0.78±0.15△（n=6） 237.01±48.23△（n=11）
[1] 陈剑秋, 石志芸, 王文健 , 等. 益气养阴活血方治疗糖尿病周围神经病的临床观察[J]. 上海中医药杂志, 2002,36(7):23-24
[2] Kato N, Makino M, Mizuno K , et al. Serial changes of sensory nerve conduction velocity and minimal F-wave latency in streptozotocin-induced diabetic rats[J]. Neurosci Lett, 1998,244(3):169-172
doi: 10.1016/S0304-3940(98)00156-6
[3] 沈守祥, 董砚虎, 逢力男 , 等. 红细胞醛糖还原酶测定荧光法的建立及临床应用[J]. 中国糖尿病杂志, 1999,7(3):154-156
[4] 周云平, 张家庆 . 醛糖还原酶(AR)的荧光光度法测定及其在糖尿病大鼠晶体AR测定中的应用[J]. 中华内分泌代谢杂志, 1989,5(3):159-161
[5] Shimoshige Y, Ikuma K, Yamamoto T , et al. The effects of zenarestat, an aldose reductase inhibitor, on peripheral neuropathy in Zucker diabetic fatty rats[J]. Metabolism, 2000,49(11):1395-1399
doi: 10.1053/meta.2000.17723
[6] Suzen S, Buyukbingol E . Recent studies of aldose reductase enzyme inhibition for diabetic complications[J]. Curr Med Chem, 2003,10(15):1329-1352
doi: 10.2174/0929867033457377
[7] Suh KS, Oh SJ, Woo JT , et al. Effect of cilostazol on the neuropathies of streptozotocin-induced diabetic rats[J]. Korean J Intern Med, 1999,14(2):34-40
doi: 10.3904/kjim.1999.14.2.34
[8] Ibrahim S, Harris ND, Radata M , et al. A new minimally invasive technique to show nerve ischaemia in diabetic neuropathy[J]. Diabetologia, 1999,42(6):737-742
doi: 10.1007/s001250051222
[9] Takahashi K, Ghatei MA, Lam HC , et al. Elevated plasma endothelin in patients with diabetes mellitus[J]. Diabetologia, 1990,33(5):306-310
doi: 10.1007/BF00403325
[10] Park JY, Takahara N, Gabriele A , et al. Induction of endothelin-1 expression by glucose: an effect of protein kinase C activation[J]. Diabetes, 2000,49(7):1239-1248
doi: 10.2337/diabetes.49.7.1239
[11] Zochodne DW, Cheng C . Diabetic peripheral nerves are susceptible to multifocal ischemic damage from endothelin[J]. Brain Res, 1999,838(1-2):11-17
doi: 10.1016/S0006-8993(99)01670-4
[1] Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[2] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[3] Dong-mei Zhang, Li-xia Lou, Ai-ming Wu, Xi-ying Lü, Zhao-juan Hu, Yan-hong Zhang, Hong-fang Liu. Effects of Astragalus membranaceus and Potentilla discolor mixture on insulin resistance and its related mRNA expressions in KKAy mice with type 2 diabetes. Journal of Chinese Integrative Medicine, 2012, 10(7): 821-826.
[4] Yan Guan , Hui Zhang, Wei Zhang, Shi-bing Su . Analysis of differential gene expression profile in peripheral blood of patients with chronic hepatitis B and syndromes of dual deficiency of liver and kidney yin and accumulation of dampness heat. Journal of Chinese Integrative Medicine, 2012, 10(7): 751-756.
[5] Lin Wang, Guan-liang Wang, Jia-han Liu, Di Li, De-zeng Zhu, Liang-neng Wu . Effects of ursolic acid in ameliorating insulin resistance in liver of KKAy mice via peroxisome proliferator-activated receptors α and γ. Journal of Chinese Integrative Medicine, 2012, 10(7): 793-799.
[6] Su-fang Zhang, Chang-quan Ling , Bai Li, Hong-yun Chen , Zhe Chen. Effects of Sisheng Decoction on the immunity and anti-stress function in mice with spleen deficiency syndrome. Journal of Chinese Integrative Medicine, 2012, 10(12): 1465-1469.
[7] Yan-qin Bian, Bing-bing Ning , Hong-yan Cao , Yan LU , Cheng Liu , Gao-feng Chen , Jia Liu , Ping Liu, Ming-yu Sun. Formula-syndrome correlation study of three classical anti-jaundice formulas in inhibition of liver fibrosis induced by dimethylnitrosamine in rats. Journal of Chinese Integrative Medicine, 2012, 10(12): 1405-1412.
[8] Hui-jiao Liang , Zhen-jiao Liang , Xiao-ping Wang. Three-dimensional reconstruction of the Lung Meridian of Hand-Taiyin with continuous thin-section CT image. Journal of Chinese Integrative Medicine, 2012, 10(12): 1400-1404.
[9] Yuan-jie Wu , Zhao-hui Fang , Shu-guo Zheng , Yuan-bo Wu , Ai-hua Fan. Effects of Chinese herbal medicine Danzhi Jiangtang Capsule and exercise on JNK signaling pathway in pancreatic tissues of diabetic rats. Journal of Chinese Integrative Medicine, 2012, 10(11): 1279-1285.
[10] You-hua Wang , Shang Li , Bin-rui Yang , Lun-qing Zhang , Xin Zhou , Bei-wen Xu, Ming-yuan Li . Use of zebrafish models for the research of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1189-1197.
[11] Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271.
[12] Xiao Zhang , Bing-bing Ning , Shuang Ren , Li-jun Zhang , Wen-meng Zhang , Jia-mei Chen , Gao-feng Chen , Hua Zhang , Yong-ping Mu , Ping Liu. Effects of Chinese herbal medicine Xiaopi Pill in preventing rats from dimethylnitrosamine-induced liver fibrosis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1286-1292.
[13] Hui Han , Li-min Wu , Ming-xiang Han, Wen-ming Yang , Dao-jun Xie , Zhao-hui Fang , Dan-chen Chu , Fang Fang . Effects of Chinese herbal medicine Shenxiong Yujing Granule on regeneration of neural cells in rats with diabetes-associated cerebral ischemia. Journal of Chinese Integrative Medicine, 2012, 10(10): 1140-1148.
[14] Cai Rong-lin, Hu Ling, Wu Zi-jian, Wang Ke-ming, He Lu, Zhou Yi-ping, Zhao Lun, Hu Wu-bin. Effects of acupuncture at Shenmen (HT7) or Taiyuan (LU9) on P3a and P3b of event-related potentials in healthy young adults. Journal of Chinese Integrative Medicine, 2012, 10(1): 48-53.
[15] Liu Yue, Xie Ming, Zhang Ye. Dynamic changes of laboratory parameters of rats with type 2 diabetes and insulin resistance: defining their role in development of traditional Chinese medicine syndrome. Journal of Chinese Integrative Medicine, 2012, 10(1): 100-108.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
[an error occurred while processing this directive]