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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (1): 31-34.doi: 10.3736/jcim20050110

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shenmai Huoxue Decoction on early diabetic peripheral neuropathy in rats

Hong-li Xue,Wen-jian Wang,Jian-qiu Chen   

  1. Institute of Chinese Integrative Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2004-05-18 Online:2005-01-20 Published:2005-01-20
  • Contact: Wen-jian Wang


To evaluate the effects of Shenmai Huoxue Decoction (SMHXD) on early diabetic peripheral neuropathy (DPN) in rats.


The rats were randomly divided into 3 groups: normal group, diabetic model group and SMHXD-treated diabetic group. The diabetic rats were induced by streptozotocin (STZ) intraperitoneally. After the treatment, sensory nerve conduction velocity (SNCV) of caudal nerve was detected with evoked electromyography, and the activity of aldose reductase (AR) in erythrocytes and the concentration of endothelin (ET) in plasma were measured.


It was showed that the SNCV reduced significantly and the activity of AR in erythrocytes and the concentration of ET in plasma were increased significantly in diabetic group as compared with those in the normal group. The SNCV was increased, and the activity of AR in erythrocytes and the concentration of ET in plasma were improved significantly in SMHXD-treated diabetic group after the treatment.


The results indicate that SMHXD can alleviate the lesion of DPN in the early stage of diabetic rats.

Key words: Shenmai Huoxue Decoction, Diabetic neuropathy, Animals, Laboratory

CLC Number: 

  • R289.5

Tab 1

Effects of SMHXD on weight and blood glucose concentration of experimental rats ($\bar{x}$±s)"

Group n Weight
Blood glucose
Normal group 11 323.82±29.30 4.01±0.56
Diabetic group 11 268.82±20.56* 31.13±3.05*
SMHXD-treated group 12 261.67±17.38* 26.97±5.44*

Tab 2

Effects of SMHXD on nerve conduction velocity of experimental rats ($\bar{x}$±s)"

Group n SNCV (m/s) Latency (ms) Amplitude (mV)
Normal group 11 34.85±3.00 0.89±0.12△ 41.05±10.83
Diabetic group 10 23.46±5.45 1.28±0.23 12.39±5.80
SMHXD-treated group 12 29.47±3.68 1.00±0.15△ 36.19±7.05

Tab 3

Effects of SMHXD on activity of AR in erythrocytes and concentration of ET in plasma in experimental rats ($\bar{x}$±s)"

Group AR (U/g Hb) ET (ng/L)
Normal group 0.76±0.14n=9) 233.20±25.46n=10)
Diabetic group 1.42±0.44 (n=10) 304.54±47.15 (n=10)
SMHXD treated group 0.78±0.15n=6) 237.01±48.23n=11)
[1] 陈剑秋, 石志芸, 王文健 , 等. 益气养阴活血方治疗糖尿病周围神经病的临床观察[J]. 上海中医药杂志, 2002,36(7):23-24
[2] Kato N, Makino M, Mizuno K , et al. Serial changes of sensory nerve conduction velocity and minimal F-wave latency in streptozotocin-induced diabetic rats[J]. Neurosci Lett, 1998,244(3):169-172
doi: 10.1016/S0304-3940(98)00156-6
[3] 沈守祥, 董砚虎, 逢力男 , 等. 红细胞醛糖还原酶测定荧光法的建立及临床应用[J]. 中国糖尿病杂志, 1999,7(3):154-156
[4] 周云平, 张家庆 . 醛糖还原酶(AR)的荧光光度法测定及其在糖尿病大鼠晶体AR测定中的应用[J]. 中华内分泌代谢杂志, 1989,5(3):159-161
[5] Shimoshige Y, Ikuma K, Yamamoto T , et al. The effects of zenarestat, an aldose reductase inhibitor, on peripheral neuropathy in Zucker diabetic fatty rats[J]. Metabolism, 2000,49(11):1395-1399
doi: 10.1053/meta.2000.17723
[6] Suzen S, Buyukbingol E . Recent studies of aldose reductase enzyme inhibition for diabetic complications[J]. Curr Med Chem, 2003,10(15):1329-1352
doi: 10.2174/0929867033457377
[7] Suh KS, Oh SJ, Woo JT , et al. Effect of cilostazol on the neuropathies of streptozotocin-induced diabetic rats[J]. Korean J Intern Med, 1999,14(2):34-40
doi: 10.3904/kjim.1999.14.2.34
[8] Ibrahim S, Harris ND, Radata M , et al. A new minimally invasive technique to show nerve ischaemia in diabetic neuropathy[J]. Diabetologia, 1999,42(6):737-742
doi: 10.1007/s001250051222
[9] Takahashi K, Ghatei MA, Lam HC , et al. Elevated plasma endothelin in patients with diabetes mellitus[J]. Diabetologia, 1990,33(5):306-310
doi: 10.1007/BF00403325
[10] Park JY, Takahara N, Gabriele A , et al. Induction of endothelin-1 expression by glucose: an effect of protein kinase C activation[J]. Diabetes, 2000,49(7):1239-1248
doi: 10.2337/diabetes.49.7.1239
[11] Zochodne DW, Cheng C . Diabetic peripheral nerves are susceptible to multifocal ischemic damage from endothelin[J]. Brain Res, 1999,838(1-2):11-17
doi: 10.1016/S0006-8993(99)01670-4
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