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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (1): 31-34.doi: 10.3736/jcim20050110

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shenmai Huoxue Decoction on early diabetic peripheral neuropathy in rats

Hong-li Xue,Wen-jian Wang,Jian-qiu Chen   

  1. Institute of Chinese Integrative Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2004-05-18 Online:2005-01-20 Published:2005-01-20
  • Contact: Wen-jian Wang E-mail:wj6518@fudan.edu.cn

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Objective

To evaluate the effects of Shenmai Huoxue Decoction (SMHXD) on early diabetic peripheral neuropathy (DPN) in rats.

Methods

The rats were randomly divided into 3 groups: normal group, diabetic model group and SMHXD-treated diabetic group. The diabetic rats were induced by streptozotocin (STZ) intraperitoneally. After the treatment, sensory nerve conduction velocity (SNCV) of caudal nerve was detected with evoked electromyography, and the activity of aldose reductase (AR) in erythrocytes and the concentration of endothelin (ET) in plasma were measured.

Results

It was showed that the SNCV reduced significantly and the activity of AR in erythrocytes and the concentration of ET in plasma were increased significantly in diabetic group as compared with those in the normal group. The SNCV was increased, and the activity of AR in erythrocytes and the concentration of ET in plasma were improved significantly in SMHXD-treated diabetic group after the treatment.

Conclusion

The results indicate that SMHXD can alleviate the lesion of DPN in the early stage of diabetic rats.

Key words: Shenmai Huoxue Decoction, Diabetic neuropathy, Animals, Laboratory

CLC Number: 

  • R289.5

Tab 1

Effects of SMHXD on weight and blood glucose concentration of experimental rats ($\bar{x}$±s)"

Group n Weight
(g)		 Blood glucose
(mmol/L)
Normal group 11 323.82±29.30 4.01±0.56
Diabetic group 11 268.82±20.56* 31.13±3.05*
SMHXD-treated group 12 261.67±17.38* 26.97±5.44*

Tab 2

Effects of SMHXD on nerve conduction velocity of experimental rats ($\bar{x}$±s)"

Group n SNCV (m/s) Latency (ms) Amplitude (mV)
Normal group 11 34.85±3.00△ 0.89±0.12△ 41.05±10.83△
Diabetic group 10 23.46±5.45 1.28±0.23 12.39±5.80
SMHXD-treated group 12 29.47±3.68△ 1.00±0.15△ 36.19±7.05△

Tab 3

Effects of SMHXD on activity of AR in erythrocytes and concentration of ET in plasma in experimental rats ($\bar{x}$±s)"

Group AR （U/g Hb） ET （ng/L）
Normal group 0.76±0.14△（n=9） 233.20±25.46△（n=10）
Diabetic group 1.42±0.44 （n=10） 304.54±47.15 （n=10）
SMHXD treated group 0.78±0.15△（n=6） 237.01±48.23△（n=11）
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