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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (1): 100-108.doi: 10.3736/jcim20120115

Special Issue: Traditional Chinese Medicine

• Original Experimental Research • Previous Articles     Next Articles

Dynamic changes of laboratory parameters of rats with type 2 diabetes and insulin resistance: defining their role in development of traditional Chinese medicine syndrome

Liu Yue1,Xie Ming2(),Zhang Ye3   

  1. 1. Center of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
    2. State Key Department of Formulaology, Beijing University of Chinese Medicine, Beijing 100029, China
    3. Department of Formulaology, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, Henan Province, China
  • Received:2011-08-17 Accepted:2011-09-07 Online:2012-01-20 Published:2018-09-21
  • Contact: Xie Ming

OBJECTIVE: To observe the development of Chinese medicine syndromes of rats with type 2 diabetes and insulin resistance by detecting dynamic changes of laboratory parameters.METHODS: A total of 110 male Sprague-Dawley rats were randomly divided into control group (n=50) and model group (n=60) to observe the correlation between laboratory parameter changes and development of Chinese medicine syndromes. A rat model of type 2 diabetes and insulin resistance was established by feeding rats with high-calorie and high-fat diet together with peritoneal injection of streptozocin (30 mg/kg, once). At the end of 0, 6, 8, 10 and 12 weeks, 10 rats were randomly selected form each group and blood was taken from common carotid artery to detect contents of total cholesterol (TC), triacylglycerol (TAG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and leptinin serum, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) contents in plasma and whole blood and plasma viscosities.RESULTS: Compared with the control group, concentrations of TNF-α, IL-6, leptin and cAMP and ratio of cAMP/cGMP increased and content of cGMP decreased markedly from the 6th week to the 12th week. Concentrations of TC, TAG and LDL-C increased from the 8th week to the 12th week, while HDL-C decreased from the 10th week to the 12th week. Whole blood and plasma viscosity values under different shear rates increased significantly from the 10th week to the 12th week.CONCLUSION: Based on the understanding of markers which have close relationship with Chinese medicine syndromes of dual deficiency of qi and yin, turbid phlegm and blood stasis, combining with the dynamic changes of appearance behavior and laboratory parameters of this animal experiment, the authors speculate the development characteristics of Chinese medicine syndromes in rats with type 2 diabetes. The dynamic progress of Chinese medicine syndromes and pathology varies from dual deficiency of qi and yin to dual deficiency of qi and yin with turbid phlegm, and at last to the combination of dual deficiency of qi and yin, turbid phlegm and blood stasis.

Key words: diabetes mellitus, type 2, insulin resistant, models, animal, syndrome, rats

Table 1

Content of serum total cholesterol in two groups at different time points ($\bar{x}$±s, mmol/L)"

Group n Total cholesterol
0 week 6 weeks 8 weeks 10 weeks 12 weeks
Control 10 0.89±0.01 0.91±0.07 0.92±0.03 1.01±0.03 1.02±0.19
Model 10 0.85±0.03 0.95±0.03 0.97±0.17 1.89±0.16*△ 2.13±0.13**△

Table 2

Content of serum triacylglycerol in two groups at different time points ($\bar{x}$±s, mmol/L)"

Group n Triacylglycerol
0 week 6 weeks 8 weeks 10 weeks 12 weeks
Control 10 0.35±0.05 0.41±0.04 0.45±0.13 0.61±0.04 0.84±0.10
Model 10 0.37±0.06 0.43±0.01 0.47±0.03 0.64±0.05 1.09±0.03*△

Table 3

Content of serum low-density lipoprotein cholesterol in two groups at different time points ($\bar{x}$±s, mmol/L)"

Low-density lipoprotein cholesterol
Group n 0 week 6 weeks 8 weeks 10 weeks 12 weeks
Control 10 0.41±0.09 0.45±0.06 0.47±0.10 0.46±0.02 0.49±0.07
Model 10 0.40±0.07 0.51±0.03 0.57±0.04 0.99±0.09*△ 1.23±0.15**△

Table 4

Content of serum high-density lipoprotein cholesterol in two groups at different time points ($\bar{x}$±s, mmol/L)"

Group n High-density lipoprotein cholesterol
0 week 6 weeks 8 weeks 10 weeks 12 weeks
Control 10 0.98±0.01 1.00±0.04 1.02±0.03 1.03±0.06 1.03±0.09
Model 10 0.99±0.02 0.97±0.03* 1.00±0.02 1.00±0.07 0.94±0.02*

Table 5

Content of serum tumor necrosis factor-α in two groups at different time points ($\bar{x}$±s, μg/mL)"

Group n Tumor necrosis factor-α
0 week 6 weeks 8 weeks 10 weeks 12 weeks
Control 10 0.76±0.18 0.85±0.16 1.01±0.14 1.02±0.16 1.06±0.09
Model 10 0.82±0.13 1.67±0.11* 1.98±0.17*△ 2.48±0.19*△ 3.11±0.18**△

Table 6

Content of serum interleukin-6 in two groups at different time points ($\bar{x}$±s, ng/L)"

Group n Interleukin-6
0 week 6 weeks 8 weeks 10 weeks 12 weeks
Control 10 101.23±19.15 98.23±18.49 111.48±12.84 93.64±15.49 113.88±10.48
Model 10 90.18±10.26 170.59±19.81* 210.88±22.59*△ 350.18±19.56**△ 420.26±22.56**△

Table 7

Content of serum leptin in two groups at different time points ($\bar{x}$±s, μg/L)"

Group n Leptin
0 week 6 weeks 8 weeks 10 weeks 12 weeks
Control 10 4.82±0.21 5.22±0.33 5.23±0.36 5.39±0.62 5.60±0.61
Model 10 4.78±0.30 6.31±0.58* 6.37±0.71* 7.72±0.66*△ 9.03±0.69*△

Table 8

Content of plasma cyclic adenosine monophosphate in two groups at different time points ($\bar{x}$±s, pmol/L)"

Group n Plasma cyclic adenosine monophosphate
0 week 6 weeks 8 weeks 10 weeks 12 weeks
Control 10 39.21±4.32 38.99±5.17 39.02±6.54 40.10±7.20 38.46±5.69
Model 10 40.19±4.23 41.24±5.94 51.28±5.66*△ 68.94±7.29*△ 72.11±6.1**△

Table 9

Content of plasma cyclic guanosine monophosphate in two groups at different time points ($\bar{x}$±s, pmol/L)"

Group n Plasma cyclic guanosine monophosphate
0 week 6 weeks 8 weeks 10 weeks 12 weeks
Control 10 11.09±0.47 11.21±0.36 11.29±0.39 11.12±0.20 11.24±0.26
Model 10 11.06±0.79 11.53±0.41 9.14±1.01*△ 8.32±1.09*△ 7.23±1.10**△

Table 10

Ratio of cAMP to cGMP in two groups at different time points ($\bar{x}$±s)"

Group n cAMP/cGMP
0 week 6 weeks 8 weeks 10 weeks 12 weeks
Control 10 3.02±0.57 3.25±0.67 3.19±0.59 3.38±0.70 3.40±0.66
Model 10 3.13±0.89 3.89±0.71 5.01±0.92* 7.99±0.98*△ 9.12±1.01**△

Table 11

Whole blood viscosity under high shear in two groups at different time points ($\bar{x}$±s, mPa·s)"

Group n Whole blood viscosity under high shear
0 week 6 weeks 8 weeks 10 weeks 12 weeks
Control 10 5.88±0.15 5.89±0.12 5.89±0.17 5.90±0.13 5.92±0.11
Model 10 5.89±0.10 5.90±0.13 5.91±0.16 5.94±0.19 6.10±0.18*△

Table 12

Whole blood viscosity under low shear in two groups at different time points ($\bar{x}$±s, mPa·s)"

Group n Whole blood viscosity under low shear
0 week 6 weeks 8 weeks 10 weeks 12 weeks
Control 10 12.78±0.11 12.92±0.14 12.04±0.12 13.04±0.33 13.22±0.21
Model 10 12.91±0.23 12.98±0.21 12.99±0.19 13.09±0.12 14.10±0.27*△

Table 13

Plasma viscosity in two groups at different time points ($\bar{x}$±s, mPa·s)"

Group n Plasma viscosity
0 week 6 weeks 8 weeks 10 weeks 12 weeks
Control 10 1.58±0.13 1.59±0.09 1.57±0.11 1.61±0.16 1.63±0.08
Model 10 1.61±0.09 1.62±0.08 1.59±0.13 1.64±0.06 1.79±0.04*△
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