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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (2): 128-131.doi: 10.3736/jcim20050213

• Original Experimental Research • Previous Articles     Next Articles

Protective effect of low-level laser irradiation on acupuncture points combined with iontophoresis against focal cerebral ischemia-reperfusion injury in rats

Ju-yun Dai1,Lin-bao Ge2,Yan-li Zhou2,Lin Wang1   

  1. 1. Acupuncture Clinic, Institute of Qigong, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China
    2. Department of Acupuncture, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2004-10-25 Online:2005-03-20 Published:2005-03-20
  • Contact: Ju-yun Dai E-mail:djysh2002@yahoo.com.cn

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Objective

To investigate the effects of low-level laser irradiation on acupuncture points combined with iontophoresis against brain damage after middle cerebral artery occlusion (MCAO) in rats.

Methods

Sixty-nine SD rats were randomly divided into five groups, including normal group, sham operation group, model group, electro-acupuncture group and low-level laser irradiation on acupuncture points combined with iontophoresis group (LLLI group). The cerebral ischemia-reperfusion (I/R) model was established by thread embolism of middle cerebral artery. The rats in the LLLI group, as well as the electro-acupuncture group were given treatment as soon as the occlusion finished (0 hour) and 12, 24 hours after the occlusion. We observed the changes of neurological deficit scores and the body weight of the rats at different time. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the rat’s brain tissue were tested.

Results

The neurological deficit score of the LLLI group was significantly lower than that of the model group. The body weight and the activity of SOD of the rats decreased slightly, and the content of MDA decreased significantly after the treatment.

Conclusion

The low-level laser irradiation on acupuncture points combined with iontophoresis can prevent focal cerebral ischemia-reperfusion injury. One of its mechanisms may be increasing the activity of SOD and decreasing the damage of the oxidation products to the body.

Key words: Laser therapy, Iontophoresis, Brain ischemia, Superoxide dismutase, Malondialdehyde

CLC Number: 

  • R454.21

Tab 1

Neurological deficit scores at different time in 5 groups ($\bar{x}$±s)"

Group n Neurological deficit scores at different time
0 h 12 h 24 h
Normal group 12 0** 0** 0**
Sham operation group 12 0** 0** 0**
Model group 13 2.69±1.65△△ 2.54±1.39△△ 2.38±1.32△△
Electro-acupuncture group 13 2.77±1.59△△ 1.85±1.68△△ 0.92±1.66**△△
LLLI group 12 3.16±1.75△△ 1.67±1.60△△ 1.00±1.70**△△

Tab 2

Changes of body weight of rats in 5 groups ($\bar{x}$±s, g)"

Group n Body weight before
treatment		 Body weight after
treatment		 Body weight difference
before and after treatment
Normal group 12 367.25±19.00 371.25±23.39 –4.00±6.45
Sham operation group 12 367.25±23.65 360.50±21.50* 6.75±7.15△
Model group 13 365.31±19.39 329.54±25.68△△▲ 35.77±14.98△△▲▲
Electro-acupuncture group 13 367.62±20.99 341.62±22.20△▲ 26.00±7.84*△△▲▲
LLLI group 12 374.25±18.03 348.25±19.43*△ 26.00±5.66*△△▲▲

Tab 3

Activities of SOD and contents of MDA in rat’s brain tissues in 5 groups ($\bar{x}$±s)"

Group n SOD (nU/mg protein) MDA (nmol/mg protein)
Normal group 12 34.78±5.56* 7.23±0.88**
Sham operation group 12 34.69±5.69* 7.28±0.98**
Model group 13 26.50±3.96 9.80±0.90△△
Electro-acupuncture group 13 33.43±5.80* 8.04±1.32**△
LLLI group 12 32.59±3.55* 7.92±0.85**
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