Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (2): 128-131.doi: 10.3736/jcim20050213

• Original Experimental Research • Previous Articles     Next Articles

Protective effect of low-level laser irradiation on acupuncture points combined with iontophoresis against focal cerebral ischemia-reperfusion injury in rats

Ju-yun Dai1,Lin-bao Ge2,Yan-li Zhou2,Lin Wang1   

  1. 1. Acupuncture Clinic, Institute of Qigong, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China
    2. Department of Acupuncture, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2004-10-25 Online:2005-03-20 Published:2005-03-20
  • Contact: Ju-yun Dai


To investigate the effects of low-level laser irradiation on acupuncture points combined with iontophoresis against brain damage after middle cerebral artery occlusion (MCAO) in rats.


Sixty-nine SD rats were randomly divided into five groups, including normal group, sham operation group, model group, electro-acupuncture group and low-level laser irradiation on acupuncture points combined with iontophoresis group (LLLI group). The cerebral ischemia-reperfusion (I/R) model was established by thread embolism of middle cerebral artery. The rats in the LLLI group, as well as the electro-acupuncture group were given treatment as soon as the occlusion finished (0 hour) and 12, 24 hours after the occlusion. We observed the changes of neurological deficit scores and the body weight of the rats at different time. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the rat’s brain tissue were tested.


The neurological deficit score of the LLLI group was significantly lower than that of the model group. The body weight and the activity of SOD of the rats decreased slightly, and the content of MDA decreased significantly after the treatment.


The low-level laser irradiation on acupuncture points combined with iontophoresis can prevent focal cerebral ischemia-reperfusion injury. One of its mechanisms may be increasing the activity of SOD and decreasing the damage of the oxidation products to the body.

Key words: Laser therapy, Iontophoresis, Brain ischemia, Superoxide dismutase, Malondialdehyde

CLC Number: 

  • R454.21

Tab 1

Neurological deficit scores at different time in 5 groups ($\bar{x}$±s)"

Group n Neurological deficit scores at different time
0 h 12 h 24 h
Normal group 12 0** 0** 0**
Sham operation group 12 0** 0** 0**
Model group 13 2.69±1.65△△ 2.54±1.39△△ 2.38±1.32△△
Electro-acupuncture group 13 2.77±1.59△△ 1.85±1.68△△ 0.92±1.66**△△
LLLI group 12 3.16±1.75△△ 1.67±1.60△△ 1.00±1.70**△△

Tab 2

Changes of body weight of rats in 5 groups ($\bar{x}$±s, g)"

Group n Body weight before
Body weight after
Body weight difference
before and after treatment
Normal group 12 367.25±19.00 371.25±23.39 –4.00±6.45
Sham operation group 12 367.25±23.65 360.50±21.50* 6.75±7.15△
Model group 13 365.31±19.39 329.54±25.68△△▲ 35.77±14.98△△▲▲
Electro-acupuncture group 13 367.62±20.99 341.62±22.20△▲ 26.00±7.84*△△▲▲
LLLI group 12 374.25±18.03 348.25±19.43* 26.00±5.66*△△▲▲

Tab 3

Activities of SOD and contents of MDA in rat’s brain tissues in 5 groups ($\bar{x}$±s)"

Group n SOD (nU/mg protein) MDA (nmol/mg protein)
Normal group 12 34.78±5.56* 7.23±0.88**
Sham operation group 12 34.69±5.69* 7.28±0.98**
Model group 13 26.50±3.96 9.80±0.90△△
Electro-acupuncture group 13 33.43±5.80* 8.04±1.32**
LLLI group 12 32.59±3.55* 7.92±0.85**
[1] Longa EZ, Weinstein RP, Carlson S , et al. Reversible middle cerebral artery occlusion:without craniectomy in rats[J]. Stroke, 1989,20(1):84-91
doi: 10.1161/01.STR.20.1.84 pmid: 2643202
[2] 张英鸽, 刘天培, 钱之玉 , 等. 人参总甙对大鼠大脑中动脉阻断所致梗塞范围和脑内多胺含量的影响[J]. 中国药理学与毒理学杂志, 1994,8(4):250-255
[3] 祝总骧 . 经络在表皮层和角质层的低阻抗特性及其形态学的研究[J]. 中国医药学报, 1988,3(5):3-5
[4] 吴洁, 戴居云 . 丹芪益心贴方药中桂皮醛经皮渗透的研究[J]. 中国学术期刊文摘, 2000,6(2):226-228
[5] 冀宏, 李澎涛, 黄启福 . 解毒通络方抗大鼠脑缺血再灌注后脂质过氧化损伤的实验研究[J]. 北京中医药大学学报, 2000,23(6):21-23
[6] Yoshida S, Inoh S, Asano T , et al. Effect of transient ischemia on free fatty acids and phospholipids in the gerbil brain. Lipid peroxidation as a possible cause of postischemic injury[J]. J Neurosurg, 1980,53(3):323-331
doi: 10.3171/jns.1980.53.3.0323
[7] Braughler JM, Hall ED . Central nervous system trauma and stroke. I. Biochemical consideration for oxygen radical formation and lipid peroxidation[J]. Free Radic Biol Med, 1989,6(3):289-301
doi: 10.1016/0891-5849(89)90056-7
[8] Butterfield JD Jr , McGraw CP.Free radical pathology[J]. Stroke, 1978,9(5):443-445
doi: 10.1161/01.STR.9.5.443
[9] 刘亚敏, 张赐安, 徐秋英 , 等. 芳香开窍法对全脑缺血再灌注大鼠脑水肿及超氧化物歧化酶、丙二醛水平的影响[J]. 中国中医药信息杂志, 2002,9(7):22-24
doi: 10.3969/j.issn.1005-5304.2002.07.010
[1] Roslinah Mohamad Hussain, Noor Faradilla Abdullah, Zulkhairi Amom. Killing of Staphylococcus aureus by allylpyrocatechol is potentiated by induction of intracellular oxidative stress and inhibition of catalase activity. Journal of Integrative Medicine, 2016, 14(6): 456-464.
[2] Xiao-ping Huang, Huang Ding, Jin-dong Lu, Ying-hong Tang, Bing-xiang Deng, Chang-qing Deng . Autophagy in cerebral ischemia and the effects of traditional Chinese medicine. Journal of Integrative Medicine, 2015, 13(5): 289-296.
[3] Sunil A. Nirmal, Jayashri M. Ingale, Shashikant R. Pattan, Sanjay B. Bhawar. Amaranthus roxburghianus root extract in combination with piperine as a potential treatment of ulcerative colitis in mice. Journal of Chinese Integrative Medicine, 2013, 11(3): 206-212.
[4] Lu Liu, Ying Gao. Study on the correlation between traditional Chinese medicine syndrome and short-term prognosis of ischemic stroke using logistic regression model and repeated-measures analysis of variance. Journal of Chinese Integrative Medicine, 2012, 10(9): 983-990.
[5] Yue-hua Wang, Xiao-li He, Xiao-xiu Li, Hai-lin Qin, Guan-hua Du . Effects of the effective component group of Chinese herbal medicine Xiaoxuming Decoction on brain mitochondria in rats with chronic cerebral ischemia. Journal of Chinese Integrative Medicine, 2012, 10(5): 569-576.
[6] Gao Shan, Wang Xing-hui, Huang Ling-ling, Yu Ting-ting, Du Su-ming, Guo Yan-wei, Jia Yuan, Wang Jian. Effects of a compound Chinese medicine Xinji’erkang on isoproterenol-induced ventricular remodeling in mice. Journal of Chinese Integrative Medicine, 2012, 10(3): 330-336.
[7] Hui Han , Li-min Wu , Ming-xiang Han, Wen-ming Yang , Dao-jun Xie , Zhao-hui Fang , Dan-chen Chu , Fang Fang . Effects of Chinese herbal medicine Shenxiong Yujing Granule on regeneration of neural cells in rats with diabetes-associated cerebral ischemia. Journal of Chinese Integrative Medicine, 2012, 10(10): 1140-1148.
[8] Qi-hong Shi , Jun Xiang, Xu-ying Zhu , Ding-fang Cai. Protective effects of Chinese herbal medicine Naoshuantong on neurovascular unit in rats with cerebral ischemia/reperfusion injury. Journal of Chinese Integrative Medicine, 2012, 10(10): 1135-1139.
[9] Yang Zhuo-xin, Chen Peng-dian, Yu Hai-bo, Luo Wen-shu, Wu Yong-gang, Pi Min, Peng Jun-hua, Liu Yong-feng, Zhang Shao-yun, Gou Yan-hua. Research advances in treatment of cerebral ischemic injury by acupuncture of conception and governor vessels to promote nerve regeneration. Journal of Chinese Integrative Medicine, 2012, 10(1): 19-24.
[10] Lei Chen, Xiao-ying Bi, Li-xun Zhu, Yi-qing Qiu, Su-ju Ding, Ben-qiang Deng. Flavonoids of puerarin versus tanshinone ⅡA for ischemic stroke: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2011, 9(11): 1215-1220.
[11] Ding-zhu Shen , Qing Ta, Jin-xing Du, Sai-dan Ding, Gao-feng Chen, Yi-yang Ru, Ping Liu. Effects of Yiguanjian Decoction on liver cirrhosis formation: A differential proteomics study in rats. Journal of Chinese Integrative Medicine, 2010, 8(2): 158-167.
[12] Jian-yu Li, Yi Li, Hai-ying Gong, Xiao-bo Zhao, Ling-zhi Li. Protective effects of n-butanol extract of Potentilla anserina on acute myocardial ischemic injury in mice. Journal of Chinese Integrative Medicine, 2009, 7(1): 48-52.
[13] Xiu-ying Wen, Qin-hui Hu, Fang Wang. Amelioration effect of Zhenqing Capsule on peripheral neuropathy in type 1 diabetic rats. Journal of Chinese Integrative Medicine, 2008, 6(3): 289-293.
[14] Xue-lin Zhang, Xiao-qiang Liang, Hong-gang Gu, En-wei Ma, Tian-bi Lin, Xun Sun, Jing-zhe Zhang. Effects of Shengqing Capsule on biochemical parameters in mice with cholesterol gallstone. Journal of Chinese Integrative Medicine, 2008, 6(10): 1045-1048.
[15] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76.
Full text



[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228