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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (1): 48-52.doi: 10.3736/jcim20090107

• Original Experimental Research • Previous Articles     Next Articles

Protective effects of n-butanol extract of Potentilla anserina on acute myocardial ischemic injury in mice

 Jian-yu Lia, Yi Lia, Hai-ying Gonga, Xiao-bo Zhaob, Ling-zhi Lia   

  1. a Division of Pharmaceutical Chemistry, Department of Preventive Medicine, Medical College of Chinese People's Armed Police Forces, Tianjin 300162, China
    b Team 061 of Department of Pharmacy, Medical College of Chinese People's Armed Police Forces, Tianjin 300162, China
  • Received:2008-05-18 Accepted:2008-07-08 Online:2009-01-20 Published:2009-01-15

Objective

To investigate the protective effects of the n-butanol extract of Potentilla anserina L. (NP) on pituitrin-induced acute myocardial ischemic injury in mice.
Methods

Ninety healthy female mice were randomly divided into normal control group, untreated group, Salvia miltiorrhiza group and low-, medium- and high-dose NP groups. Except for the normal control group, the mice were intraperitoneally injected with pituitrin (20 U/kg) to induce acute myocardial ischemic injury. Thirty minutes after induction, electrocardiogram was monitored, and height of the J spot was measured also. Activities of lactate dehydrogenase (LDH), creatine kinase (CK) and superoxide dismutase (SOD) and content of malondialdehyde (MDA) in serum of the mice were detected. The degree of myocardial ischemic injury in mice was observed by Nagar-Olsen staining.
Results

The moving up of J spots in the treated groups was significantly inhibited when comparing with the untreated group (P<0.01). Compared with untreated group, high- and medium-dose NP and Salvia miltiorrhiza could significantly decrease the activities of LDH, CK (P<0.01, P<0.05), increase the SOD activity (P<0.01) and decrease the content of MDA (P<0.05). However, no significant difference was observed between low-dose NP group and untreated group (P>0.05). Nagar-Olsen staining showed that high- and medium-dose NP and Salvia miltiorrhiza could significantly diminish the areas of cardiac muscles injured by ischemia, but low-dose NP had no effect on that.
Conclusion

NP has a remarkable protective effect on acute myocardial ischemic injury in mice.

Key words: Potentilla anserina L., Myocardial ischemia, Superoxide dismutase, Creatine kinase, Lactate dehydrogenase, Malondialdehyde, Mice

Table 1

Effects of NP on J spot change of electrocardiogram in mice with myocardial ischemic injury (x±s, mV)"

Group n J spot height
Normal control 15 0.020±0.022
Untreated 15 0.223±0.043**
Salvia miltiorrhiza 15 0.100±0.055**△△
Low-dose NP 15 0.145±0.016**△△
Medium-dose NP 15 0.111±0.034**△△
High-dose NP 15 0.105±0.038**△△

Table 2

Effects of NP on activities of serum LDH and CK in mice with myocardial ischemic injury (x±s, U/L)"

Group n LDH CK
Normal control 15 1 651±755 366±193
Untreated 15 5 769±827** 2 208±258**
Salvia miltiorrhiza 15 4 277±878**△△ 1 304±360**△△
Low-dose NP 15 5 848±788** 2 880±712**
Medium-dose NP 15 4 752±766**△△ 1 382±360**
High-dose NP 15 2 650±917**△△ 715±340*△△

Table 3

Effects of NP on SOD activity and MDA content in serum of mice with myocardial ischemic injury (x±s)"

Group n SOD (kU/L) MDA (μmol/L)
Normal control 15 312.864±32.132 4.369±0.316
Untreated 15 208.781±33.425** 6.216±0.382**
Salvia miltiorrhiza 15 303.220±31.672△△ 5.405±0.382**△△
Low-dose NP 15 209.432±35.423** 6.081±0.506**
Medium-dose NP 15 244.920±37.423**△△ 5.586±0.221**△△
High-dose NP 15 288.621±43.346*△△ 4.595±0.483△△

Figure 1

Nagar-Olsen staining of cardiac muscles of mice in different groups (Light microscopy, ×200) A: Normal control group; B: Untreated group; C: Salvia miltiorrhiza group; D: Low-dose NP group; E: Medium-dose NP group; F: High-dose NP group."

[1] Ma CH . Study on chemical constituents and pharmacologic action of Potentilla anserina L. in Tibetan medicine[J]. Shi Zhen Guo Yi Guo Yao, 2006,17(8):1584-1585
马春花 . 藏药蕨麻的化学成分及药理作用研究概况[J]. 时珍国医国药, 2006,17(8):1584-1585
[2] Li LZ, Zhang L, Gong HY, Zhao C, Zhang L, Zhu Y, Li GC, Chen Y, Li JY . Study on anti-hypoxia and anti-oxidation effects of Potentilla anserina L. alcohol extract[J]. Zhongguo Shi Pin Wei Sheng Za Zhi, 2005,17(4):306-308
李灵芝, 张丽, 龚海英, 赵赤, 张岭, 朱毅, 李广策, 陈洋, 李建宇 . 蕨麻醇提取物的抗缺氧及抗氧化机制研究[J]. 中国食品卫生杂志, 2005,17(4):306-308
[3] Li JY, Li LZ, Zhang XN, Li Y . Protective effect of Potentilla anserina L. alcohol extract on cardiac muscles injured by chronic hypoxia[J]. Wei Sheng Yan Jiu, 2007,36(6):740-742
李建宇, 李灵芝, 张新宁, 李怡 . 蕨麻醇提物对小鼠慢性缺氧心肌损伤的保护作用[J]. 卫生研究, 2007,36(6):740-742
[4] Li JY, Li LZ, Zhang YL, Zhang XN, Gong HY . Protective effect of Potentilla anserina L. on hypoxia injury in neonatal rats cardiomyocytes[J]. Zhongguo Xin Yao Za Zhi, 2007,16(12):944-946
李建宇, 李灵芝, 张永亮, 张新宁, 龚海英 . 蕨麻醇提物对心肌细胞缺氧损伤的保护作用[J]. 中国新药杂志, 2007,16(12):944-946
[5] Li JY, Li LZ, Gong HY, Li Y, Zhang XN . Effect of Potentilla anserina L. on cardiomyocyte apoptosis induced by hypoxia[J]. Zhonghua Lao Nian Xin Nao Xue Guan Bing Za Zhi, 2007,9(9):619-622
李建宇, 李灵芝, 龚海英, 李怡, 张新宁 . 蕨麻对缺氧诱导心肌细胞凋亡的影响[J]. 中华老年心脑血管病杂志, 2007,9(9):619-622
[6] Wang YH, Ma SP . Protective effect of Xin Tai Capusle on acute myocardial ischemic injury induced by pituitrin in rats[J]. Zhongguo Yao Li Yu Lin Chuang, 2007,23(2):52-54
王宇华, 马世平 . 心泰胶囊对垂体后叶素诱发大鼠急性心肌缺血的保护作用[J]. 中国药理与临床, 2007,23(2):52-54
[7] Wang QJ, Pan ZW, Yue P, Kong LY . Effects of total flavones from Herba Epimedii on experimental myocardial ischemia and hemorheology of animals[J]. Zhong Cao Yao, 2007,38(7):1039-1042
王秋娟, 潘志伟, 岳攀, 孔令义 . 淫羊藿总黄酮对动物实验性心肌缺血及血液流变学的影响[J]. 中草药, 2007,38(7):1039-1042
[8] Wang ZW, Hong T, Yang Z . Protective effect of Anxin on pituitrin-induced myocardial ischemic injury[J]. Shi Zhen Guo Yi Guo Yao, 2007,18(1):81-82
王智文, 洪铁, 杨振 . 安心对垂体后叶素所致实验性心肌缺血的保护作用[J]. 时珍国医国药, 2007,18(1):81-82
[9] Zhou Q, Lu DX, Fu YM, Wang HD . Glycine protection against the myocardial ischemic injury in mice[J]. Zhongguo Bing Li Sheng Li Za Zhi, 2002,18(4):360-362
周琼, 陆大祥, 付咏梅, 王华东 . 甘氨酸对小鼠心肌缺血性损伤的防治作用研究[J]. 中国病理生理杂志, 2002,18(4):360-362
[10] Lu GY, Chen SH, Li LW, Zhang XD, Wang LX, Fu HY, Lou ZH, Wu J . Effect of HQSM on CK, LDH and AST in serum of myocardial ischemia rats[J]. Zhejiang Zhong Yi Yao Da Xue Xue Bao, 2006,30(2):208-210
吕圭源, 陈素红, 李立文, 张晓东, 王丽霞, 傅惠英, 楼招欢, 吴晶 . 黄芪生脉饮有效部位对心肌缺血大鼠血清CK、LDH、AST的影响[J]. 浙江中医药大学学报, 2006,30(2):208-210
[11] Wang BY. Technology of pathology[M]. Beijing: People's Medical Publishing House,2001: 149
王伯沄 . 病理学技术[M]. 北京: 人民卫生出版社,2001: 149
[12] Xie CX, Yang YQ, Lu JP, Tang M, Zhou W . Protective effect of Yimucao(Herba leonuri) injection against cerebral ischemia: an experimental study in mice and rats[J]. Nan Fang Yi Ke Da Xue Xue Bao, 2007,27(10):1528-1530
谢崇宣, 杨育群, 泸健沛, 唐冕, 周伟 . 益母草注射液对小鼠及大鼠实验性脑缺血的保护作用[J]. 南方医科大学学报, 2007,27(10):1528-1530
[13] Xie Y, Chen SQ, Li ZP . Research advances in the protective effect of traditional Chinese medicine on myocardial damage[J]. Zhong Xi Yi Jie He Xin Nao Xue Guan Bing Za Zhi, 2007,5(10):980-981
谢赟, 陈寿权, 李章平 . 中药对心肌损伤保护作用的研究进展[J]. 中西医结合心脑血管病杂志, 2007,5(10):980-981
[14] Gao M . Study on the protective effect of traditional Chinese medicine on myocardial ischemia-reperfusion injury[J]. Tianjin Yao Xue, 2007,19(4):60-63
高濛 . 心肌缺血再灌注损伤的中药保护研究[J]. 天津药学, 2007,19(4):60-63
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