蕨麻正丁醇提取部位对小鼠急性心肌缺血损伤的保护作用

doi:10.3736/jcim20090107

Abstract

Objective

To investigate the protective effects of the n-butanol extract of Potentilla anserina L. (NP) on pituitrin-induced acute myocardial ischemic injury in mice.
Methods

Ninety healthy female mice were randomly divided into normal control group, untreated group, Salvia miltiorrhiza group and low-, medium- and high-dose NP groups. Except for the normal control group, the mice were intraperitoneally injected with pituitrin (20 U/kg) to induce acute myocardial ischemic injury. Thirty minutes after induction, electrocardiogram was monitored, and height of the J spot was measured also. Activities of lactate dehydrogenase (LDH), creatine kinase (CK) and superoxide dismutase (SOD) and content of malondialdehyde (MDA) in serum of the mice were detected. The degree of myocardial ischemic injury in mice was observed by Nagar-Olsen staining.
Results

The moving up of J spots in the treated groups was significantly inhibited when comparing with the untreated group (P<0.01). Compared with untreated group, high- and medium-dose NP and Salvia miltiorrhiza could significantly decrease the activities of LDH, CK (P<0.01, P<0.05), increase the SOD activity (P<0.01) and decrease the content of MDA (P<0.05). However, no significant difference was observed between low-dose NP group and untreated group (P>0.05). Nagar-Olsen staining showed that high- and medium-dose NP and Salvia miltiorrhiza could significantly diminish the areas of cardiac muscles injured by ischemia, but low-dose NP had no effect on that.
Conclusion

NP has a remarkable protective effect on acute myocardial ischemic injury in mice.

Key words: Potentilla anserina L., Myocardial ischemia, Superoxide dismutase, Creatine kinase, Lactate dehydrogenase, Malondialdehyde, Mice

Jian-yu Li, Yi Li, Hai-ying Gong, Xiao-bo Zhao, Ling-zhi Li. Protective effects of n-butanol extract of Potentilla anserina on acute myocardial ischemic injury in mice. Journal of Chinese Integrative Medicine, 2009, 7(1): 48-52.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20090107

http://www.jcimjournal.com/EN/Y2009/V7/I1/48

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Figures/Tables 4

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References 14

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Group	n	J spot height
Normal control	15	0.020±0.022
Untreated	15	0.223±0.043**
Salvia miltiorrhiza	15	0.100±0.055**^△△
Low-dose NP	15	0.145±0.016**^△△
Medium-dose NP	15	0.111±0.034**^△△
High-dose NP	15	0.105±0.038**^△△

Group	n	LDH	CK
Normal control	15	1 651±755	366±193
Untreated	15	5 769±827**	2 208±258**
Salvia miltiorrhiza	15	4 277±878**^△△	1 304±360**^△△
Low-dose NP	15	5 848±788**	2 880±712**
Medium-dose NP	15	4 752±766**^△△	1 382±360**^△
High-dose NP	15	2 650±917**^△△	715±340*^△△

Group	n	SOD (kU/L)	MDA (μmol/L)
Normal control	15	312.864±32.132	4.369±0.316
Untreated	15	208.781±33.425**	6.216±0.382**
Salvia miltiorrhiza	15	303.220±31.672^△△	5.405±0.382**^△△
Low-dose NP	15	209.432±35.423**	6.081±0.506**
Medium-dose NP	15	244.920±37.423**^△△	5.586±0.221**^△△
High-dose NP	15	288.621±43.346*^△△	4.595±0.483^△△

Protective effects of n-butanol extract of Potentilla anserina on acute myocardial ischemic injury in mice

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