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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (4): 286-288.doi: 10.3736/jcim20050411

• Original Experimental Research • Previous Articles     Next Articles

Effects of salvianolic acid B on expressions of TGF-β1 and its receptors in liver of rats with dimethylnitrosamine-induced hepatic fibrosis

Xiao-ning Wang1,Yi-yang Hu1,Cheng-hai Liu1,Ping Liu1,Da-yuan Zhu2   

  1. 1. Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
  • Received:2005-03-18 Online:2005-07-20 Published:2005-07-20
  • Contact: Yi-yang Hu E-mail:yyhu@citiz.net

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Objective

To study the therapeutic mechanism of salvianolic acid B (SA-B) in treating hepatic fibrosis.

Methods

Twenty-four Wistar rats were randomly divided into 3 groups: normal control group, untreated group and SA-B-treated group. Rats in the untreated and SA-B-treated groups were injected intraperitoneally with 0.5% dimethylnitrosamine (DMN) for 4 weeks, daily for 3 days each week at a dose of 10 μg/kg, to induce hepatic fibrosis. Then, rats in the SA-B-treated group were given SA-B orally for another 4 weeks. Hydroxyproline (Hyp) contents in liver tissue of the rats in 3 groups were determined with HCl hydrolysis, and collagen typeⅠ, transforming growth factor-beta 1 (TGF-β1), transforming growth factor-beta receptor typeⅠ(TβRⅠ) and transforming growth factor-beta receptor typeⅡ(TβRⅡ) were detected by Western blotting.

Results

The Hyp content and the expressions of collagen typeⅠ, TGF-β1, TβRⅠ and TβRⅡ in the liver tissue of rats in the untreated group increased significantly as compared with those in the normal control group, while those in SA-B-treated group decreased significantly as compared with those in the untreated group.

Conclusion

The therapeutic mechanism of SA-B in treating hepatic fibrosis may be related to inhibiting the expressions of TGF-β1 and its receptors.

Key words: Liver fibrosis, Dimethylnitrosamine, Salvianolic acid B, TGF-β1, Receptor

CLC Number: 

  • R285.5

Tab 1

Content of Hyp in liver tissue of rats ($\bar{x}$±s, mg/g)"

Group n Hyp
Normal control 8 4.01 ± 0.29
Untreated 8 9.67 ± 2.98**
SA-B-treated 8 4.55 ± 0.57△△

Tab 2

Scanning density integrals of expressions of collagen typeⅠ, TGF-β1, TβRⅠ and TβRⅡ in liver tissue of rats ($\bar{x}$±s, ％)"

Group n Collagen typeⅠ TGF-β1 TβRⅠ TβRⅡ
Normal control 3 8.00±0.17 15.23±3.20 13.20±1.25 12.50±0.17
Untreated 3 32.27±0.15** 27.30±3.26** 29.07±0.67** 32.67±1.57**
SA-B-treated 3 18.33±0.29△△ 20.50±0.36△△ 21.23±1.59△△ 18.57±0.49△△

Fig 1

Expressions of collagen typeⅠ, TGF-β1, TβRⅠ and TβRⅡ in liver tissue of rats (Western blotting) A: Collagen typeⅠ; B: TGF-β1; C: TβRⅠ; D: TβRⅡ; 1. Normal control group; 2. Untreated group; 3. SA-B-treated group"

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