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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (4): 286-288.doi: 10.3736/jcim20050411

• Original Experimental Research • Previous Articles     Next Articles

Effects of salvianolic acid B on expressions of TGF-β1 and its receptors in liver of rats with dimethylnitrosamine-induced hepatic fibrosis

Xiao-ning Wang1,Yi-yang Hu1,Cheng-hai Liu1,Ping Liu1,Da-yuan Zhu2   

  1. 1. Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
  • Received:2005-03-18 Online:2005-07-20 Published:2005-07-20
  • Contact: Yi-yang Hu E-mail:yyhu@citiz.net

Objective

To study the therapeutic mechanism of salvianolic acid B (SA-B) in treating hepatic fibrosis.

Methods

Twenty-four Wistar rats were randomly divided into 3 groups: normal control group, untreated group and SA-B-treated group. Rats in the untreated and SA-B-treated groups were injected intraperitoneally with 0.5% dimethylnitrosamine (DMN) for 4 weeks, daily for 3 days each week at a dose of 10 μg/kg, to induce hepatic fibrosis. Then, rats in the SA-B-treated group were given SA-B orally for another 4 weeks. Hydroxyproline (Hyp) contents in liver tissue of the rats in 3 groups were determined with HCl hydrolysis, and collagen typeⅠ, transforming growth factor-beta 1 (TGF-β1), transforming growth factor-beta receptor typeⅠ(TβRⅠ) and transforming growth factor-beta receptor typeⅡ(TβRⅡ) were detected by Western blotting.

Results

The Hyp content and the expressions of collagen typeⅠ, TGF-β1, TβRⅠ and TβRⅡ in the liver tissue of rats in the untreated group increased significantly as compared with those in the normal control group, while those in SA-B-treated group decreased significantly as compared with those in the untreated group.

Conclusion

The therapeutic mechanism of SA-B in treating hepatic fibrosis may be related to inhibiting the expressions of TGF-β1 and its receptors.

Key words: Liver fibrosis, Dimethylnitrosamine, Salvianolic acid B, TGF-β1, Receptor

CLC Number: 

  • R285.5

Tab 1

Content of Hyp in liver tissue of rats ($\bar{x}$±s, mg/g)"

Group n Hyp
Normal control 8 4.01 ± 0.29
Untreated 8 9.67 ± 2.98**
SA-B-treated 8 4.55 ± 0.57△△

Tab 2

Scanning density integrals of expressions of collagen typeⅠ, TGF-β1, TβRⅠ and TβRⅡ in liver tissue of rats ($\bar{x}$±s, %)"

Group n Collagen typeⅠ TGF-β1 TβRⅠ TβRⅡ
Normal control 3 8.00±0.17 15.23±3.20 13.20±1.25 12.50±0.17
Untreated 3 32.27±0.15** 27.30±3.26** 29.07±0.67** 32.67±1.57**
SA-B-treated 3 18.33±0.29△△ 20.50±0.36△△ 21.23±1.59△△ 18.57±0.49△△

Fig 1

Expressions of collagen typeⅠ, TGF-β1, TβRⅠ and TβRⅡ in liver tissue of rats (Western blotting) A: Collagen typeⅠ; B: TGF-β1; C: TβRⅠ; D: TβRⅡ; 1. Normal control group; 2. Untreated group; 3. SA-B-treated group"

[1] 刘平, 沟口靖弘, 森泽成司 . 镁-丹酚酸B抗大鼠实验性肝纤维化的作用[J]. 中国医药学报, 1993,8(增刊):65-67
[2] 胡义扬, 刘平, 刘成 , 等. 丹参提取物对CCl4和DMN诱导的大鼠肝纤维化的影响[J]. 上海中医药杂志, 1999,40(10):7-10
[3] 徐列明, 刘成, 刘平 . 丹参酚性酸B对大鼠传代肝贮脂细胞增殖形态和合成细胞外基质的影响[J]. 中华肝脏病杂志, 1996,4(2):86-89
[4] 胡义扬, 王海南, 刘成海 , 等. 丹酚酸B盐对肝星状细胞TGFβ1信号转导的影响[J]. 中国学术期刊文摘, 2001,7(5):664
[5] 王海南, 胡义扬, 洪嘉禾 , 等 .丹酚酸B盐对肝星状细胞增殖和TGFβ1信号转导的影响[J].中华肝脏病杂志, 2002, 10(5): 382, 394
[6] Liu P, Hu YY, Liu C, Zhu DY , et al. Clinical observation of salvianolic acid B in treatment of liver fibrosis in chronic hepatitis B[J]. World J Gastroenterol, 2002,8(4):679-685
doi: 10.3748/wjg.v8.i4.679
[7] Casu A, Canepa M, Nanni G . Perisinusoidal stellate cells or Ito cells and their role in hepatic fibrosis[J]. Pathologica, 1994,86(5):467-499
pmid: 7739873
[8] Jamall IS, Finelli VN, Que Hee SS . A simple method to determine nanogram levels of 4-hydroxyproline in biological tissues[J]. Anal Biochem, 1981,112(1):70-75
doi: 10.1016/0003-2697(81)90261-X pmid: 7258630
[9] 周亚军, 殷冬梅, 陈宏山 , 等. 转化生长因子β信号传导阻断对鼠肝星状细胞培养激活的影响[J]. 中华肝脏病杂志, 2003,11(5):282-284
[10] 刘成海, 刘平, 胡义扬 , 等. 丹酚酸B盐对转化生长因子-β1刺激肝星状细胞活化与胞内信号转导的作用[J]. 中华医学杂志, 2002,82(18):1267-1272
doi: 10.3760/j:issn:0376-2491.2002.18.014
[11] 赵俊芳, 刘成, 刘成海 . 转化生长因子β胞内信号转导与Smads蛋白[J]. 中国病理生理杂志, 2002,18(3):321-325
[12] 吕品, 程凤凤, 陈惠华 , 等. TGF-β1与肝纤维化[J]. 科学技术与工程, 2004,4(4):307-311
doi: 10.3969/j.issn.1671-1815.2004.04.015
[13] 郭顺根, 戴敏, 张玮 , 等. 肝纤维化发生发展与信号转导途径[J]. 中国组织化学与细胞化学杂志, 2003,12(1):106-111
[14] 陶艳艳, 刘成海 . 丹参及其化学成分抗肝纤维化作用机制研究进展[J]. 中西医结合学报, 2004,2(2):145-148
[15] 徐新保, 何振平, 梁志新 , 等. 阻断转化生长因子-β1信号传导治疗大鼠实验性肝纤维化[J]. 中华肝脏病杂志, 2004,12(5):263-266
doi: 10.3760/j.issn:1007-3418.2004.05.003
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