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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (4): 282-285.doi: 10.3736/jcim20050410

• Original Experimental Research • Previous Articles     Next Articles

Effects of Xuanyinning Recipe on invasion of SPC-A-1 cells and pathomorphological changes of peritoneum in mice inoculated with sarcoma 180

Zhen-ye Xu1,Jian-wen Liu2,Mei-yun Zhou3   

  1. 1. Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, China
    3. Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
  • Received:2004-09-23 Online:2005-07-20 Published:2005-07-20
  • Contact: Zhen-ye Xu E-mail:xuzhenye@csco.org.cn

Objective

To observe the effects of Xuanyinning Recipe (XYNR) in inhibiting SPC-A-1 cellular infiltration and on the pathomorphological changes of peritoneum in mice inoculated with sarcoma 180 (S180).

Methods

On the bases of isolated culture of mouse peritoneal mesothelial cells, we adjusted and added the human lung adenocarcinoma cell line SPC-A-1 into the double-layer culture medium to observe the number of clones formed. We also took out the peritoneum from the mice administered with three different dosages of XYNR and observed its pathomorphological changes with transmission electron microscope.

Results

In the in vitro experiment, the number of clones of SPC-A-1 in culture medium with XYNR (50 μg/ml) decreased distinctly. In the in vivo experiment, it was observed that, in the peritoneum from the XYNR-treated mice inoculated with S180, the mesothelial cells arranged more and more regularly with the increasing of the dosage of XYNR, while the mesothelial cells in the peritoneum of the mice in the control group necrosed and arranged loosely.

Conclusion

XYNR can inhibit the invasion of SPC-A-1 cells. It also can improve the loose arrangement of the peritoneal mesothelial cells in mice inoculated with S180, so as to inhibit the malignant effusion.

Key words: Xuanyinning Recipe, SPC-A-1 cells, Mesothelial cells, Sarcoma 180, Mice

CLC Number: 

  • R286.91

Fig 1

Mouse peritoneal mesothelial cells cultured on a single layer (×100)"

Fig 2

SPC-A-1 cell clones in control culture medium (×100)"

Fig 3

SPC-A-1 cell clones in culture medium with 50 μg/ml XYNR (×100)"

Fig 4

Micrograph of peritoneum observed from transmission electron microscopy A: Normal control mouse (HE staining, ×6 000); B: NS-treated mouse inoculated with S180 (HE staining, ×10 000); C: High dosage XYNR-treated mouse inoculated with S180 (HE staining, ×10 000)"

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