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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (6): 450-454.doi: 10.3736/jcim20050608

• Original Clinical Research • Previous Articles     Next Articles

Effects of flavones extracted from Portulaca oleracea on ability of hypoxia tolerance in mice and its mechanism

Li-wei Dong, Wan-yin Wang, Yi-tian Yue, Min Li   

  1. Department of Military Hygiene, Second Military Medical University, Shanghai 200433, China
  • Received:2005-06-29 Online:2005-11-20 Published:2005-11-20
  • Contact: Min Li E-mail:linlimin115@hotmail.com

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Objective

To identify anti-hypoxia ingredients extracted from Portulaca oleracea and to find out the possible mechanism of its anti-hypoxia actions.

Methods

Seventy mice were randomly divided into seven groups which were untreated (normal saline), ginsenosides-treated, polysaccharide-treated, acidic components-treated, basic components-treated, alkaloids-treated and flavones-treated groups, and the ingredients of polysaccharide, acidic components, basic components, alkaloids and flavones were extracted from Portulaca olerace. The mice in each group were fed with corresponding ingredients for one week respectively. Then the survival time of mice in hypoxic conditions was observed. Another 90 mice were divided into 3 groups: untreated (normal saline), ginsenosides-treated and flavones-treated groups. The mice in each of these 3 groups were divided into 3 subgroups according to 12-, 24- and 36-hour exposure to hypoxia (10% oxygen and 90% nitrogen), respectively. After exposure to hypoxia, the red blood cell count (RBC), hemoglobin (Hb) concentration and hematocrit (HCT) in mice were determined. The plasma erythropoietin (EPO) levels of mice were detected by enzyme-linked immunosorbent assay (ELISA) and the relative values of EPO mRNA in renal tissue and pallium of mice were determined by reverse transcriptase-polymerase chain reaction (RT-PCR).

Results

The survival time of mice in hypoxic conditions in flavones-treated group was significantly longer than that in the untreated group. The RBC, Hb concentration, HCT, plasma EPO level and the relative values of EPO mRNA in renal tissue and pallium of mice were significantly higher in the flavones-treated group than those in the untreated group.

Conclusion

The anti-hypoxia ingredients extracted from Portulaca oleracea are flavones and the anti-hypoxia effects may be obtained by improving the expression level of EPO and accelerating the generations of erythrocyte and Hb.

Key words: Portulaca oleracea, Flavone, anoxia, Hemoglobins, Erythropoietin

CLC Number: 

  • R285.5

Tab 1

Comparisons of body weights of mice before exposure to hypoxia and survival time in hypoxic condition in different groups ($\bar{x}$±s )"

Group n Body weight (g) Survival time in hypoxic condition (min)
Untreated 10 23.3±4.35 37.18±9.41
Polysaccharide-treated 10 27.2±3.11 41.03±7.44
Acidic components-treated 10 26.0±1.63 37.83±12.31
Basic components-treated 10 26.1±3.48 38.12±8.34
Alkaloids-treated 10 26.4±1.90 39.23±10.29
Flavones-treated 10 23.9±0.88 50.49±10.49**△▲
Ginsenosides-treated 10 24.7±2.76 46.63±11.02*

Tab 2

Effects of flavones extracted from Portulaca oleracea on RBC, Hb concentration, HCT and plasma EPO level in mice exposed to hypoxia for different hours ($\bar{x}$±s )"

Group n RBC (×1012/L) Hb (g/L) HCT (L/L) Plasma EPO (kU/L)
Untreated
12-hour exposure to hypoxia 10 7.23±0.72 122.1±14.9 0.388±0.02 0.170±0.016
24-hour exposure to hypoxia 10 7.11±0.64 117.5±13.3 0.388±0.03 0.327±0.020
36-hour exposure to hypoxia 10 6.90±0.90 127.5±5.7 0.401±0.02 0.379±0.026
Ginsenosides-treated
12-hour exposure to hypoxia 10 7.92±0.52* 133.9±10.4 0.405±0.03 0.187±0.024
24-hour exposure to hypoxia 10 7.70±0.68 126.2±12.6 0.416±0.02* 0.359±0.031*
36-hour exposure to hypoxia 10 7.79±0.47* 134.1±6.7* 0.435±0.01** 0.384±0.028
Flavones-treated
12-hour exposure to hypoxia 10 8.14±0.53* 129.3±14.2 0.423±0.01* 0.202±0.012*
24-hour exposure to hypoxia 10 7.99±0.42** 129.3±9.30* 0.423±0.01* 0.381±0.045*
36-hour exposure to hypoxia 10 7.87±0.54* 130.9±6.17 0.422±0.02* 0.426±0.040**

Fig 1

Effects of flavones extracted from Portulaca oleracea on expression level of EPO mRNA in renal tissues of mice exposed to hypoxia for different hours 1: Flavones-treated group; 2: Ginsenosides-treated group; 3: Untreated group"

Tab 3

Effects of flavones extracted from Portulaca oleracea on relative values of EPO mRNA in renal tissues and pallium of mice exposed to hypoxia for different hours ($\bar{x}$±s )"

Group n EPO mRNA/β-actin
Renal tissues Pallium
Untreated
12-hour exposure to hypoxia 10 0.71±0.03 0.85±0.01
24-hour exposure to hypoxia 10 0.80±0.01 0.80±0.02
36-hour exposure to hypoxia 10 0.73±0.02 0.89±0.03
Ginsenosides-treated
12-hour exposure to hypoxia 10 0.93±0.08* 0.93±0.02*
24-hour exposure to hypoxia 10 1.01±0.04** 0.85±0.03*
36-hour exposure to hypoxia 10 0.78±0.02* 0.94±0.01*
Flavones-treated
12-hour exposure to hypoxia 10 0.83±0.04* 0.92±0.02*
24-hour exposure to hypoxia 10 0.88±0.02* 0.86±0.03*
36-hour exposure to hypoxia 10 0.77±0.02* 0.98±0.02**

Fig 2

Effects of flavones extracted from Portulaca oleracea on expression level of EPO mRNA in pallium of mice exposed to hypoxia for different hours 1: Flavones-treated group; 2: Ginsenosides-treated group; 3: Untreated group"

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