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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (9): 939-941.doi: 10.3736/jcim20080912

• Original Experimental Research • Previous Articles     Next Articles

Effects of Pollen Typhae total flavones on mRNA expressions of peroxisome proliferator-activated receptors in 3T3-L1 adipocytes

Yan-ming He, Wen-jian Wang(), Wua-wei Chen, Jian Ying   

  1. Institute of Chinese Integrative Medicine, Fudan University, Shanghai 200040, China
  • Received:2008-01-24 Online:2008-09-20 Published:2008-09-15

Objective: To explore the effects of Pollen Typhae total flavones (PTF) on the mRNA expressions of peroxisome proliferator-activated receptors (PPARs) α, γ, β/δ so as to analyze its possible mechanism in improving the insulin sensitivity of 3T3-L1 adipocytes.
Methods: Adipocytes were treated with PTF, and expressions of PPARα、PPARγ、PPARβ/δ mRNAs relating to the adipocyte glucose and lipid metabolism were determined by reverse transcription polymerase chain reaction.
Results: PTF obviously up-regulated the expressions of PPARα and PPARγ mRNAs, all showing significant differences as compared with those in the normal control group (P<0.01).
Conclusion: With its function as an insulin sensitizer, PTF may enhance the PPARα and PPARγ mRNA expressions in 3T3-L1 adipocytes.

Key words: 3T3-L1 adipocyte, Pollen Typhae total flavones, free fatty acid, peroxisome proliferator-activated receptors

CLC Number: 

  • R285.5

Table 1

Expressions of PPARα, PPARγ and PPARβ/δ mRNAs of 3T3-L1 adipocytes (x±s, copies/106 GAPDH)"

Group n PPARα mRNA PPARγ mRNA PPARβ/δ mRNA
Normal control 6 1.65×104±284.36 1.29×104±637.93 1.71×104±113.78
ROS 6 2.73×104±1 039.77** 2.12×104±421.56** 1.85×104±248.82**
PTF 6 2.06×104±810.48**△△ 1.83×104±335.84**△△ 1.33×104±350.68**△△
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