蒲黄总黄酮对3T3-L1脂肪细胞过氧化物酶体增生物激活受体家族mRNA基因表达的影响

doi:10.3736/jcim20080912

Abstract

Objective: To explore the effects of Pollen Typhae total flavones (PTF) on the mRNA expressions of peroxisome proliferator-activated receptors (PPARs) α, γ, β/δ so as to analyze its possible mechanism in improving the insulin sensitivity of 3T3-L1 adipocytes.
Methods: Adipocytes were treated with PTF, and expressions of PPARα、PPARγ、PPARβ/δ mRNAs relating to the adipocyte glucose and lipid metabolism were determined by reverse transcription polymerase chain reaction.
Results: PTF obviously up-regulated the expressions of PPARα and PPARγ mRNAs, all showing significant differences as compared with those in the normal control group (P<0.01).
Conclusion: With its function as an insulin sensitizer, PTF may enhance the PPARα and PPARγ mRNA expressions in 3T3-L1 adipocytes.

Key words: 3T3-L1 adipocyte, Pollen Typhae total flavones, free fatty acid, peroxisome proliferator-activated receptors

CLC Number:

R285.5

Yan-ming He, Wen-jian Wang, Wua-wei Chen, Jian Ying. Effects of Pollen Typhae total flavones on mRNA expressions of peroxisome proliferator-activated receptors in 3T3-L1 adipocytes. Journal of Chinese Integrative Medicine, 2008, 6(9): 939-941.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20080912

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Group	n	PPARα mRNA	PPARγ mRNA	PPARβ/δ mRNA
Normal control	6	1.65×104±284.36	1.29×104±637.93	1.71×104±113.78
ROS	6	2.73×104±1 039.77**	2.12×104±421.56**	1.85×104±248.82**
PTF	6	2.06×104±810.48**^△△	1.83×104±335.84**^△△	1.33×104±350.68**^△△

Effects of Pollen Typhae total flavones on mRNA expressions of peroxisome proliferator-activated receptors in 3T3-L1 adipocytes

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