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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (5): 488-491.doi: 10.3736/jcim20080511

• Original Experimental Research • Previous Articles     Next Articles

Pollen Typhae total flavones inhibit expression of interleukin-6 in C2C12 skeletal muscle cells cultured with palmitate

Shao-Ying Lou, Yi Liu, Wei-Hua Chen, Jian Ying, Yan-Ming He, Wen-jian Wang()   

  1. Institution of Chinese Integrative Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2008-03-14 Online:2008-05-20 Published:2008-05-15
  • Contact: WANG Wen-jian

Objective: To observe the effects of Pollen Typhae total flavones (PTF) on expression of interleukin-6 (IL-6) mRNA and protein secretion in C2C12 cell strain of skeletal muscle cells cultured with palmitate, and to explore the mechanism of PTF in relieving insulin resistance (IR).
Methods: The IR of C2C12 cells was induced by co-culturing with palmitate. The C2C12 cells were divided into normal control group, untreated group, PDTC (a nuclear factor-kappaB inhibitor) treated group, rosiglitazone (ROS)-treated group, ROS+ PDTC -treated group, PTF-treated group and PTF+PDTC-treated group. Sixteen hours after culture, the transportation rate of glucose was observed by 3H-deoxyglucose uptake method; IL-6 mRNA expression in C2C12 cells was assayed by real time polymerase chain reaction (Real-time PCR), and level of IL-6 protein secretion in culture supernatant was detected by enzyme linked immunosorbent assay.
Results: Compared with the normal control group, the transportation rate of glucose of cells in untreated group was decreased 30.43% after 16-hour palmitate culture, and was increased 32.39% in the PTF-treated group. Compared with the untreated group, the levels of IL-6 mRNA expression in cells and IL-6 protein secretion in supernatant were significantly decreased in the PTF-treated group (P<0.05). The levels of IL-6 mRNA expression in cells and IL-6 protein secretion in supernatant were increased in PTF+PDTC-treated group as compared with PFT-treated group (P<0.05).
Conclusion: PTF can inhibit the IL-6 mRNA expression and IL-6 protein secretion via nuclear factor-kappaB pathway in C2C12 skeletal muscle cells, which may be one of its mechanisms in relieving inflammation conditions and insulin resistance in C2C12 skeletal muscle cells.

Key words: Pollen Typhae total flavones, palmitate, insulin resistance, interleukin-6, C2C12 cell strain

CLC Number: 

  • R285.5

Figure 1

Expression of IL-6 mRNA in C2C12 skeletal muscle cells in different groups **P<0.01, vs normal control group; △P<0.05, △△P<0.01, vs model group; ▲P<0.05, vs PTF-treated group. □P<0.05, vs ROS-treated group; ■P<0.05, vs model+P DTC-treated group. PDTC- refers to no PDTC, PDTC+ refers to PDTC added, n=6."

Figure 2

Contents of IL-6 protein in culture supernatant of C2C12 skeletal muscle cells in different groups **P<0.01, vs normal control group; △△P<0.01, vs model group; ▲P<0.05, vs PTF-treated group; □P<0.05, vs ROS-treated group. PDTC-refers to no PDTC, PDTC+ refers to PDTC added, n=6."

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