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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (5): 488-491.doi: 10.3736/jcim20080511

• Original Experimental Research • Previous Articles     Next Articles

Pollen Typhae total flavones inhibit expression of interleukin-6 in C2C12 skeletal muscle cells cultured with palmitate

Shao-Ying Lou, Yi Liu, Wei-Hua Chen, Jian Ying, Yan-Ming He, Wen-jian Wang()   

  1. Institution of Chinese Integrative Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2008-03-14 Online:2008-05-20 Published:2008-05-15
  • Contact: WANG Wen-jian E-mail:wj6518@163.com

Objective: To observe the effects of Pollen Typhae total flavones (PTF) on expression of interleukin-6 (IL-6) mRNA and protein secretion in C2C12 cell strain of skeletal muscle cells cultured with palmitate, and to explore the mechanism of PTF in relieving insulin resistance (IR).
Methods: The IR of C2C12 cells was induced by co-culturing with palmitate. The C2C12 cells were divided into normal control group, untreated group, PDTC (a nuclear factor-kappaB inhibitor) treated group, rosiglitazone (ROS)-treated group, ROS+ PDTC -treated group, PTF-treated group and PTF+PDTC-treated group. Sixteen hours after culture, the transportation rate of glucose was observed by 3H-deoxyglucose uptake method; IL-6 mRNA expression in C2C12 cells was assayed by real time polymerase chain reaction (Real-time PCR), and level of IL-6 protein secretion in culture supernatant was detected by enzyme linked immunosorbent assay.
Results: Compared with the normal control group, the transportation rate of glucose of cells in untreated group was decreased 30.43% after 16-hour palmitate culture, and was increased 32.39% in the PTF-treated group. Compared with the untreated group, the levels of IL-6 mRNA expression in cells and IL-6 protein secretion in supernatant were significantly decreased in the PTF-treated group (P<0.05). The levels of IL-6 mRNA expression in cells and IL-6 protein secretion in supernatant were increased in PTF+PDTC-treated group as compared with PFT-treated group (P<0.05).
Conclusion: PTF can inhibit the IL-6 mRNA expression and IL-6 protein secretion via nuclear factor-kappaB pathway in C2C12 skeletal muscle cells, which may be one of its mechanisms in relieving inflammation conditions and insulin resistance in C2C12 skeletal muscle cells.

Key words: Pollen Typhae total flavones, palmitate, insulin resistance, interleukin-6, C2C12 cell strain

CLC Number: 

  • R285.5

Figure 1

Expression of IL-6 mRNA in C2C12 skeletal muscle cells in different groups **P<0.01, vs normal control group; △P<0.05, △△P<0.01, vs model group; ▲P<0.05, vs PTF-treated group. □P<0.05, vs ROS-treated group; ■P<0.05, vs model+P DTC-treated group. PDTC- refers to no PDTC, PDTC+ refers to PDTC added, n=6."

Figure 2

Contents of IL-6 protein in culture supernatant of C2C12 skeletal muscle cells in different groups **P<0.01, vs normal control group; △△P<0.01, vs model group; ▲P<0.05, vs PTF-treated group; □P<0.05, vs ROS-treated group. PDTC-refers to no PDTC, PDTC+ refers to PDTC added, n=6."

[1] Hirabara SM, Silveira LR, Abdulkader F , et al. Time-dependent effects of fatty acids on skeletal muscle metabolism[J]. J Cell Physiol, 2007,210(1):7-15
doi: 10.1002/jcp.20811 pmid: 17013887
[2] de Luca C, Olefsky JM . de Luca C, Olefsky JM. Inflammation and insulin resistance[J]. FEBS Lett, 2008,582(1):97-105
doi: 10.1016/j.febslet.2007.11.057
[3] Bruce CR, Dyck DJ . Cytokine regulation of skeletal muscle fatty acid metabolism: effect of interleukin-6 and tumor necrosis factor-alpha[J]. Am J Physiol Endocrinol Metab, 2004,287(4):E616-621
doi: 10.1152/ajpendo.00150.2004
[4] Kem PA, Ranganathan S, Li C , et al. Adipose tissue tumor necrosis factor and interleukin-6 expression in human obesity and insulin resistance[J]. Am J Physiol Endocrinol Metab, 2001,280(5):E745-751
doi: 10.1152/ajpendo.2001.280.5.E745
[5] Jové M, Planavila A, Laguna JC , et al. Palmitate-induced interleukin 6 production is mediated by protein kinase C and nuclear-factor kappaB activation and leads to glucose transporter 4 down-regulation in skeletal muscle cells[J]. Endocrinology, 2005,146(7):3087-3095
doi: 10.1210/en.2004-1560
[6] Jové M, Planavila A, Sánchez RM , et al. Palmitate induces tumor necrosis factor-alpha expression in C2C12 skeletal muscle cells by a mechanism involving protein kinase C and nuclear factor-kappaB activation[J]. Endocrinology, 2006,147(1):552-561
doi: 10.1210/en.2005-0440
[7] Reaven GM, Chen YD . Role of insulin in regulation of lipoprotein metabolism in diabetes[J]. Diabetes Metab Rev, 1988,4(7):639-652
doi: 10.1002/dmr.5610040703 pmid: 3069396
[8] Itani SI, Zhou Q, Pories WJ , et al. Involvement of protein kinase C in human skeletal muscle insulin resistance and obesity[J]. Diabetes, 2000,49(8):1353-1358
doi: 10.2337/diabetes.49.8.1353 pmid: 10923637
[9] Pradhan AD, Manson JE, Rifai N , et al. C-reactive protein, interleukin 6, and risk of developing type 2 diabetes mellitus[J]. JAMA, 2001,286(3):327-334
doi: 10.1001/jama.286.3.327 pmid: 11466099
[10] Yan H, Chen PD, Ding AW . Advancement in research of preparation of Pollen Typhae and its chemical components and pharmacological effect[J]. Jiangsu Zhong Yi YaoJiangsu Zhong Yi Yao, 2005,26(1):59-61
doi: 10.3969/j.issn.1672-397X.2005.01.030
严辉, 陈佩东, 丁安伟 . 蒲黄的炮制及其化学成分、药理作用的研究进展[J]. 江苏中医药, 2005,26(1):59-61
doi: 10.3969/j.issn.1672-397X.2005.01.030
[11] He YM, Wang WJ, Chen WH , et al. Effects of Pollen Typhae total flavone on glucose and lipid metabolism in 3T3-L1 adipocytes[J]. Zhong Xi Yi Jie He Xue Bao, 2006,4(6):593-595
何燕铭, 王文健, 陈伟华 , 等. 蒲黄总黄酮对3T3-L1脂肪细胞糖脂代谢的影响[J]. 中西医结合学报, 2006,4(6):593-595
[12] Zhu MJ . Clinical observation on effect of Pollen Typhae in treating 60 cases of hyperlipidemia[J]. Jiujiang Yi Xue, 2002,17(4):218
朱铭金 . 生蒲黄治疗高脂血症60例疗效观察[J]. 九江医学, 2002,17(4):218
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