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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (3): 307-310.doi: 10.3736/jcim20060318

• Original Experimental Research • Previous Articles     Next Articles

Effects of Kangfengshi Granules on expressions of osteoprotegerin, RANKL and M-CSF in bone tissues of rats with collagen-induced arthritis

Yi-heng Liu1, Hai-ying Zhang2, Hong-min Zang1, Jun-chang Cheng1, Yan-min Li3   

  1. 1. Department of Orthopedics, Second Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi Province 710004, China;
    2. Departmentof Anatomy , Medical College, Xi’an Jiaotong University , Xi’an , Shaanxi Province 710061 , China
    3. 3 . Department of Orthopedics ,Affiliated Hospital , Shaanxi College of Traditional Chinese Medicine, Xianyang, Shaanxi Province 712083 , China
  • Online:2006-05-31 Published:2006-05-15

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Objective

To observe the effects of Kangfengshi Granules (KFSG) on expressions of the mRNAs of osteoprotegerin (OPG), receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony stimulating factor (M-CSF) in bone tissues of rats with collagen-induced arthritis.

Methods

Forty SD rats were randomly divided into four groups: normal control group, untreated group, cyclosporine A (CsA)-treated group and KFSG-treated group. Except the rats in the normal control group, all the other rats received subcutaneous injection of collagen Ⅱ to establish collagen-induced arthritis (CIA) models. Then the rats in each group were fed normal saline or corresponding drugs for four weeks. Total RNA was extracted from carpal and digital bones. The expressions of OPG, RANKL and M-CSF mRNAs were examined by real-time PCR.

Results

The total incidence of arthritis induced by collagen Ⅱ in the rats was approximately 90%. The expression levels of RANKL and M-CSF mRNAs and the RANKL mRNA/OPG mRNA ratio in the untreated group, KFSG-treated group and CsA-treated group were all significantly higher than those in the normal control group, while the expression levels of OPG mRNA in those three groups were significantly lower than that in the normal control group. The expression level of OPG mRNA in the KFSG-treated group was obviously higher while the expression level of M-CSF mRNA and the RANKL mRNA/OPG mRNA ratio in the same group were both lower as compared with those in the untreated group.

Conclusion

The molecular mechanism of effects of KFSG on bone erosion and destruction induced by rheumatoid arthritis is closely correlated with up-regulating the expression of OPG mRNA, down-regulating the expression of M-CSF mRNA and RANKL mRNA/OPG mRNA ratio.

Key words: Kangfengshi Granules, Arthritis, Osteoprotegerin, Receptor activator of nuclear factor-κB ligand, Macrophage colony stimulating factor

CLC Number: 

  • R684.3

Table 1

Onset time of collagen-induced arthritis in rats in different groups (ヌ±S )"

Group n Onset time
Untreated group 10 17.47±2.11
KFSG-treated group 10 24.25±1.86**
CsA-treated group 10 27.82±2.86**△△

Table 2

Arthritis index of rats with collagen-induced arthritis at different time-point of treatment in different groups (ヌ±S)"

Group n Arthritis index
15th day 20th day 25th day 30th day 35th day
Untreated group 10 3.17±0.54 5.12±0.81 7.73±1.18 9.14±1.63 10.73±1.42
KFSG-treated group 10 2.93±0.82 3.79±0.96** 4.25±1.04** 6.17±1.14** 7.89±0.97**
CsA-treated group 10 2.72±0.62* 3.54±0.49** 3.48±0.89**△ 3.42±0.72**△△ 3.38±0.79**△△

Table 3

Expression levels of OPG, RANKL and M-CSF mRNAs and RANKL mRNA/OPG mRNA ratio in rats in different groups (ヌ±S)"

Group n OPG mRNA RANKL mRNA RANKL mRNA/OPG mRNA M-CSF mRNA
Normal control group 10 1.226±0.45 1.215±0.39 0.969±0.29 1.382±0.41
Untreated group 10 0.264±0.14* 5.648±0.71* 21.273±5.18* 3.143±0.65*
KFSG-treated group 10 0.653±0.15*△ 5.518±0.29* 8.481±2.38*△ 1.559±0.55*△
CsA-treated group 10 0.279±0.26* 5.547±0.86* 19.335±3.96* 2.336±1.15*△
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