抗风湿颗粒对胶原诱导关节炎大鼠骨保护素、核因子-κB受体激活因子配体和巨噬细胞集落刺激因子表达的影响

doi:10.3736/jcim20060318

Abstract

Objective

To observe the effects of Kangfengshi Granules (KFSG) on expressions of the mRNAs of osteoprotegerin (OPG), receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony stimulating factor (M-CSF) in bone tissues of rats with collagen-induced arthritis.

Methods

Forty SD rats were randomly divided into four groups: normal control group, untreated group, cyclosporine A (CsA)-treated group and KFSG-treated group. Except the rats in the normal control group, all the other rats received subcutaneous injection of collagen Ⅱ to establish collagen-induced arthritis (CIA) models. Then the rats in each group were fed normal saline or corresponding drugs for four weeks. Total RNA was extracted from carpal and digital bones. The expressions of OPG, RANKL and M-CSF mRNAs were examined by real-time PCR.

Results

The total incidence of arthritis induced by collagen Ⅱ in the rats was approximately 90%. The expression levels of RANKL and M-CSF mRNAs and the RANKL mRNA/OPG mRNA ratio in the untreated group, KFSG-treated group and CsA-treated group were all significantly higher than those in the normal control group, while the expression levels of OPG mRNA in those three groups were significantly lower than that in the normal control group. The expression level of OPG mRNA in the KFSG-treated group was obviously higher while the expression level of M-CSF mRNA and the RANKL mRNA/OPG mRNA ratio in the same group were both lower as compared with those in the untreated group.

Conclusion

The molecular mechanism of effects of KFSG on bone erosion and destruction induced by rheumatoid arthritis is closely correlated with up-regulating the expression of OPG mRNA, down-regulating the expression of M-CSF mRNA and RANKL mRNA/OPG mRNA ratio.

Key words: Kangfengshi Granules, Arthritis, Osteoprotegerin, Receptor activator of nuclear factor-κB ligand, Macrophage colony stimulating factor

CLC Number:

R684.3

Yi-heng Liu, Hai-ying Zhang, Hong-min Zang, Jun-chang Cheng, Yan-min Li. Effects of Kangfengshi Granules on expressions of osteoprotegerin, RANKL and M-CSF in bone tissues of rats with collagen-induced arthritis. Journal of Chinese Integrative Medicine, 2006, 4(3): 307-310.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20060318

http://www.jcimjournal.com/EN/Y2006/V4/I3/307

TrendMD

Figures/Tables 3

Table 1

Table 2

Table 3

References 11

[1]	Ono Y, Ogihara Y, Saijo S , et al. Evaluation of Kampo medicines used to treat rheumatoid arthritis in collagen-induced arthritic and pX transgenic mice[J]. Mod Rheumatol, 2003,13(2):50-56
	Ono Y, Ogihara Y, Saijo S , et al. Evaluation of Kampo medicines used to treat rheumatoid arthritis in collagen-induced arthritic and pX transgenic mice[J]. Mod Rheumatol, 2003,13(2):50-56
[2]	韩晓枫, 马宝骊, 张继英 , 等. 鸡Ⅱ型胶原诱导大鼠类风关模型的建立[J]. 上海免疫学杂志, 2002,21(6):330-333
	韩晓枫, 马宝骊, 张继英 , 等. 鸡Ⅱ型胶原诱导大鼠类风关模型的建立[J]. 上海免疫学杂志, 2002,21(6):330-333
[3]	Yin JL, Shackel NA, Zekry A , et al. Real-time reverse transcriptase polymerase chain reaction(RT-PCT) for measurement of cytokine and growth factor mRNA expression with fluorogenic probes or SYBR Green I[J]. Immunol Cell Biol, 2001,79(3):213-221
[4]	Feng X . Regulatory roles and molecular signaling of TNF family members in osteoclasts[J]. Gene, 2005,350(1):1-13 doi: 10.1016/j.gene.2005.01.014 pmid: 15777737
[5]	Wittrant Y, Theoleyre S, Couillaud S , et al. Regulation of osteoclast protease expression by RANKL[J]. Biochem Biophys Res Commun, 2003,310(3):774-778 doi: 10.1016/j.bbrc.2003.09.084 pmid: 14550270
[6]	Saidenberg KN, Corrado A, Lemeiter D , et al. TNF-α antibodies and osteoprotegerin decrease systemic bone loss associated with inflammation through distinct mechanisms in collagen-induced arthritis[J]. Bone, 2004,35(5):1200-1207 doi: 10.1016/j.bone.2004.07.004
[7]	Saidenberg KN, Cohen SM, Bessis N , et al. Role for osteoprotegerin in rheumatoid inflammation[J]. Joint Bone Spine, 2004,71(1):9-13 doi: 10.1016/S1297-319X(03)00131-3 pmid: 14769514
[8]	Sajti E, van Meeteren N, Kavelaars A , et al. Individual differences in behavior of inbred Lewis rats are associated with severity of joint destruction in adjuvant-induced arthritis[J]. Brain Behav Immun, 2004,18(6):505-514 doi: 10.1016/j.bbi.2003.12.004
[9]	田代华 . 实用中药辞典(第1版)[M]. 北京: 人民卫生出版社,2002,(第1版):
[10]	高宗强, 郭荣 . 抗风湿颗粒抗炎镇痛作用的实验研究[J]. 山西中医学院学报, 2003,4(3):19-21
[11]	杨锋, 李彦民, 李引刚 , 等. 仙龙颗粒对大鼠佐剂性关节炎T淋巴细胞亚群的影响[J]. 中医正骨, 2005,17(3):12-13

Related Articles 15

[1]	Chiung-Hui Huang, Mei-Ling Yeh, Fang-Pey Chen, Daphne Wu. Low-level laser acupuncture reduces postoperative pain and morphine consumption in older patients with total knee arthroplasty: A randomized placebo-controlled trial. Journal of Integrative Medicine, 2022, 20(4): 321-328.
[2]	Ye-juan Jia, Tian-yu Li, Peng Han, Yu Chen, Li-jia Pan, Chun-sheng Jia. Effects of different courses of moxibustion treatment on intestinal flora and inflammation of a rat model of knee osteoarthritis. Journal of Integrative Medicine, 2022, 20(2): 173-181.
[3]	Ju-Yeon Park, Young-Won Kwon, Sun-Ah Kim, Sun-Dong Park, Chang-Hyun Kim, Jin-Hee Kim, Ju-Hee Lee. Polyherbal formula SC-E3 inhibits rheumatoid arthritis activity in a mouse model of type-II collagen-induced arthritis. Journal of Integrative Medicine, 2021, 19(3): 265-273.
[4]	Johannes Loeser, Brigitte Layer, Christopher Plata, Klaus-Maria Perrar, Tim Hucho, Rebecca Kulbida. Hirudotherapy attenuates arthritic pain in patients with various chronic pain syndromes: A retrospective analysis. Journal of Integrative Medicine, 2020, 18(5): 425-433.
[5]	Sandip B. Bharate, Vikas Kumar, Sonali S. Bharate, Bikarma Singh, Gurdarshan Singh, Amarinder Singh, Mehak Gupta, DeepikaSingh, AjayKumar, Surjeet Singh, Ram A. Vishwakarma. Discovery and preclinical development of IIIM-160, a Bergenia ciliata-based anti-inflammatory and anti-arthritic botanical drug candidate. Journal of Integrative Medicine, 2019, 17(3): 192-204.
[6]	Katy Andrade Monteiro Zacaron, Nélia da Silva Mendes, Yuri Cotta e Silva, Giancarlo Lucchetti. Effects of laying on of hands with and without a spiritual context on pain and functionality of older women with knee osteoarthritis: Study protocol for a randomized controlled trial. Journal of Integrative Medicine, 2018, 16(2): 106-112.
[7]	Kyeong Mee Park, Tae Hwan Cho. Therapeutic effect of acupuncture point injection with placental extract in knee osteoarthritis. Journal of Integrative Medicine, 2017, 15(2): 135-141.
[8]	Susana Seca, Sebastian Kirch, António S.Cabrita, Henry J.Greten. Evaluation of the effect of acupuncture on hand pain, functional deficits and health-related quality of life in patients with rheumatoid arthritis—A study protocol for a multicenter, double-blind, randomized clinical trial. Journal of Integrative Medicine, 2016, 14(3): 219-227.
[9]	MesfinYimam, Young-Chul Lee, Breanna Moore, Ping Jiao, MeiHong, Jeong-Bum Nam, Mi-Ran Kim, Eu-Jin Hyun, Min Chu, Lidia Brownell, Qi Jia. Analgesic and anti-inflammatory effects of UP1304, a botanical composite containing standardized extracts of Curcuma longa and Morus alba. Journal of Integrative Medicine, 2016, 14(1): 60-68.
[10]	Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[11]	Jian Liu, Chuan-bing Huang, Yuan Wang, Gui-qin Xu, Yuan-yuan Cheng, Yun-xia Feng, Lei Liu, Ya-jun Qi. Chinese herbal medicine Xinfeng Capsule in treatment of rheumatoid arthritis: Study protocol of a multicenter randomized controlled trial. Journal of Chinese Integrative Medicine, 2013, 11(6): 428-434.
[12]	Martin Schencking, Stefan Wilm, Marcus Redaelli. A comparison of Kneipp hydrotherapy with conventional physiotherapy in the treatment of osteoarthritis: A pilot trial. Journal of Chinese Integrative Medicine, 2013, 11(1): 17-25.
[13]	Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[14]	Gui-lin Ouyang, Xiao-hui Feng, Lian-bo Xiao, Zheng Huang, Qing Xia, Da-peng Han, Fei Zhu . Effects of Chinese herbal medicine Qianggu Capsule on patients with rheumatoid arthritis-induced osteoporosis: a report of 82 cases. Journal of Chinese Integrative Medicine, 2012, 10(12): 1394-1399.
[15]	Nian-hong Wang , Jun-tao Yan , Wu-quan Sun , Yong-shan Hu , Jun Xia , Li-cheng Wei , Jie Jia , Gui-lin Ouyang , Yong He , Yan-ming Guo , Jie Xu . Effects of early application of Tuina treatment on quadriceps surface myoelectricity in patients after total knee arthroplasty: a randomized controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(11): 1247-1253.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2]	Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3]	Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4]	SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5]	Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6]	Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7]	Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8]	Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9]	Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10]	Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239

Group	n	Onset time
Untreated group	10	17.47±2.11
KFSG-treated group	10	24.25±1.86**
CsA-treated group	10	27.82±2.86**^△△

Group	n	Arthritis index
Group	n	15th day	20th day	25th day	30th day	35th day
Untreated group	10	3.17±0.54	5.12±0.81	7.73±1.18	9.14±1.63	10.73±1.42
KFSG-treated group	10	2.93±0.82	3.79±0.96**	4.25±1.04**	6.17±1.14**	7.89±0.97**
CsA-treated group	10	2.72±0.62*	3.54±0.49**	3.48±0.89**^△	3.42±0.72**^△△	3.38±0.79**^△△

Group	n	OPG mRNA	RANKL mRNA	RANKL mRNA/OPG mRNA	M-CSF mRNA
Normal control group	10	1.226±0.45	1.215±0.39	0.969±0.29	1.382±0.41
Untreated group	10	0.264±0.14*	5.648±0.71*	21.273±5.18*	3.143±0.65*
KFSG-treated group	10	0.653±0.15*△	5.518±0.29*	8.481±2.38*^△	1.559±0.55*^△
CsA-treated group	10	0.279±0.26*	5.547±0.86*	19.335±3.96*	2.336±1.15^*△

Effects of Kangfengshi Granules on expressions of osteoprotegerin, RANKL and M-CSF in bone tissues of rats with collagen-induced arthritis

RichHTML

PDF (PC)

Like