Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (2): 185-188.doi: 10.3736/jcim20060215

• Original Experimental Research • Previous Articles     Next Articles

Effects of compound preparation of Cordyceps sinensis and Tripterygium hypoglaucum on survival time of pigskin after allogeneic transplantation

Dai-wei Cheng1, Yong Zou2, Ning Qian3, Chao-liang Wang2, Ying-biao Tian2, Da-li Wang2, Gui-xiang Zhao1, Zhen-yu Gao2   

  1. 1. Department of Burn and Plastic Surgery, Guizhou People’s Hospital, Guiyang, Guizhou Province 550002, China;
    2. First Hospital, Zunyi Medical College, Zunyi, Guizhou Province 563003, China
    3. Center for Animal Laboratory, Guiyang College of Traditional Chinese Medicine, Guiyang, Guizhou Province 550000, China
  • Online:2006-03-20 Published:2006-03-15


To investigate the effects of compound preparation of Cordyceps sinensis and Tripterygium hypoglaucum (CSTHC) on survival time of grafted pigskin after allogeneic transplantation and its mechanism.


The pigskin was treated with CSTHC solution before allogeneic transplantation, and CSTHC ointment was applied for external use on the grafted pigskin after skin transplantation. Cyclosporine A (CsA) and normal saline were served as control. The survival time, the appearance and the histomorphological changes of the grafted pigskin were observed. The histomorphological changes of testicles in pigs were also examined. The CD4 and CD8 expressions in the grafted pigskins were measured by immunohistochemical method. The white blood cell count in peripheral blood and the liver and renal functions were also examined. 


The survival time of the grafted pigskin in the CSTHC-treated group was (28.50±3.26) d, which was much longer as compared with (10.60±1.52) d in the untreated group (P<0.01). The survival time of the grafted pigskin in the CsA-treated group was (28.33±3.50) d, and there was no remarkable difference in the survival time of the grafted pigskin between the CsA-treated group and the CSTHC-treated group. The expressions of CD4 and CD8 were lower in the CSTHC-treated group than those in the untreated group on the 7th and 14th day after skin graft (P<0.05), while there was no significant difference in the indices between the CSTHC-treated group and the CsA-treated group. The WBC count was higher in the untreated group than that in the CSTHC-treated group or CsA-treated group on the 7th day after skin graft (P<0.05). 


CSTHC can prolong the survival time of allogeneic grafted pigskin. Its mechanism of inhibiting the immunological rejection may relate to decreasing the expressions of CD4+ and CD8+ in the grafted pigskin and reducing the local inflammatory reaction.

Key words: Cordyceps sinensis, Allograft, Skin transplantation, Tissue survival, Immunological rejection

CLC Number: 

  • R622.1

Figure 1

Grafted pigskin appearance in untreated group on the 14th day after allogeneic transplantation"

Figure 2

Grafted pigskin appearance in CSTHC-treated group on the 14th day after allogeneic transplantation"

Figure 3

Histomorphological changes of grafted pigskin in untreated group on the 14th day after allogeneic transplantation (HE staining, ×400)"

Figure 4

Histomorphological changes of grafted pigskin in CSTHC-treated group on the 14th day after allogeneic transplantation (HE staining, ×400)"

Table 1

Numbers of CD4+ and CD8+ T cells in grafted pigskin before and after allogeneic transplantation in 3 groups (ヌ±S)"

GroupnNumber of CD4+ T cellsNumber of CD8+ T cells
Before transplantation60.79±0.620.66±0.34
7 d after transplantation63.46±1.20*2.58±0.41*
14 d after transplantation66.58±1.66*5.33±0.79*
28 d after transplantation67.58±1.937.33±1.01
Before transplantation60.83±0.470.83±0.68
7 d after transplantation63.58±1.00*3.38±0.47*
14 d after transplantation66.75±2.02*5.75±0.84*
28 d after transplantation68.08±2.327.17±0.58
Before transplantation50.65±0.260.79±0.66
7 d after transplantation58.30±1.82*6.80±1.22*
14 d after transplantation58.25±1.47*7.95±0.93*
28 d after transplantation5
[1] 龚非力. 同种器官移植免疫耐受的研究进展[J].中国器官移植杂志,1998,19(3):129
doi: 10.3760/cma.j.issn.0254-1785.1998.03.001
[2] 何长民, 石炳毅.器官移植免疫学(第1版)[M].北京: 人民军医出版社,1995,(第1版):29-31
[3] 陆佩华. 异种移植研究[J].中国器官移植杂志,1998,19(1):61-62
[4] 黎鳌, 杨宗成, 萧光夏, 等.实验烧伤外科学(第1版)[M].重庆: 重庆大学出版社,1997,(第1版):432-436
[5] 林素文, 刘延深, 林宜衍, 等.冬虫夏草及中国拟青霉对机体细胞免疫功能的调节作用[J].中成药研究,1987,12(1):22-23
[6] 汪道武, 黄明明.虫草类的免疫药理研究Ⅴ:蝙蝠蛾拟青霉人工发酵培养物对小鼠T细胞及其亚群功能的影响[J].同济医科大学学报,1998,27(5):332-334
[7] 邓文龙, 聂仁吉, 刘家玉.昆明山海棠的药理作用研究Ⅱ:对免疫功能的影响[J].中草药,1981,12(10):26-30
[8] 陈梓璋, 胡尧碧, 温志坚, 等.昆明山海棠提取物的毒理实验[J].生殖与避孕,1990,10(4):56-57
[9] 程代薇, 罗志军, 邹勇, 等.冬虫夏草-山海棠复方制剂对小鼠混合淋巴细胞培养中PCNA、IL-2表达的影响[J].中华烧伤杂志,2002,18(4):244
[10] Bach FH, Auchincloss H.Transplantation immunology(第1版)[M].New York: John Wiley & Sons,1995,(第1版):339-346
[11] Rowbottom AW, Lepper MA, Garland RJ, et al.Interleukin-10 induced CD8 cell proliferation[J].Immunology,1999,98(1):80-89
doi: 10.1046/j.1365-2567.1999.00828.x pmid: 10469237
[12] 于艳秋, 王亚杰, 高杰, 等.高压氧与甲基强地松龙对不同系小鼠皮肤移植局部浸润T淋巴细胞及细胞黏附分子表达的影响[J].中国医科大学学报,2000,29(2):99-100
[1] Wen Sun, Jian Yu, Yu-min Shi, Hao Zhang, Ying Wang, Bing-bing Wu. Effects of Cordyceps extract on cytokines and transcription factors in peripheral blood mononuclear cells of asthmatic children during remission stage. Journal of Chinese Integrative Medicine, 2010, 8(4): 341-346.
Full text



[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228