复方虫草制剂延长猪同种异体移植皮存活期的实验研究

doi:10.3736/jcim20060215

Abstract

Objective

To investigate the effects of compound preparation of Cordyceps sinensis and Tripterygium hypoglaucum (CSTHC) on survival time of grafted pigskin after allogeneic transplantation and its mechanism.

Methods

The pigskin was treated with CSTHC solution before allogeneic transplantation, and CSTHC ointment was applied for external use on the grafted pigskin after skin transplantation. Cyclosporine A (CsA) and normal saline were served as control. The survival time, the appearance and the histomorphological changes of the grafted pigskin were observed. The histomorphological changes of testicles in pigs were also examined. The CD4 and CD8 expressions in the grafted pigskins were measured by immunohistochemical method. The white blood cell count in peripheral blood and the liver and renal functions were also examined.

Results

The survival time of the grafted pigskin in the CSTHC-treated group was (28.50±3.26) d, which was much longer as compared with (10.60±1.52) d in the untreated group (P＜0.01). The survival time of the grafted pigskin in the CsA-treated group was (28.33±3.50) d, and there was no remarkable difference in the survival time of the grafted pigskin between the CsA-treated group and the CSTHC-treated group. The expressions of CD4 and CD8 were lower in the CSTHC-treated group than those in the untreated group on the 7th and 14th day after skin graft (P＜0.05), while there was no significant difference in the indices between the CSTHC-treated group and the CsA-treated group. The WBC count was higher in the untreated group than that in the CSTHC-treated group or CsA-treated group on the 7th day after skin graft (P＜0.05).

Conclusion

CSTHC can prolong the survival time of allogeneic grafted pigskin. Its mechanism of inhibiting the immunological rejection may relate to decreasing the expressions of CD4⁺ and CD8⁺ in the grafted pigskin and reducing the local inflammatory reaction.

Key words: Cordyceps sinensis, Allograft, Skin transplantation, Tissue survival, Immunological rejection

CLC Number:

R622.1

Dai-wei Cheng, Yong Zou, Ning Qian, Chao-liang Wang, Ying-biao Tian, Da-li Wang, Gui-xiang Zhao, Zhen-yu Gao. Effects of compound preparation of Cordyceps sinensis and Tripterygium hypoglaucum on survival time of pigskin after allogeneic transplantation. Journal of Chinese Integrative Medicine, 2006, 4(2): 185-188.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20060215

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References 12

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Group	n	Number of CD4⁺T cells	Number of CD8⁺ T cells
CSTHC-treated
Before transplantation	6	0.79±0.62	0.66±0.34
7 d after transplantation	6	3.46±1.20*^△	2.58±0.41*^△
14 d after transplantation	6	6.58±1.66*^△	5.33±0.79*^△
28 d after transplantation	6	7.58±1.93	7.33±1.01
CsA-treated
Before transplantation	6	0.83±0.47	0.83±0.68
7 d after transplantation	6	3.58±1.00*^△	3.38±0.47*^△
14 d after transplantation	6	6.75±2.02*^△	5.75±0.84*^△
28 d after transplantation	6	8.08±2.32	7.17±0.58
Untreated
Before transplantation	5	0.65±0.26	0.79±0.66
7 d after transplantation	5	8.30±1.82*	6.80±1.22*
14 d after transplantation	5	8.25±1.47*	7.95±0.93*
28 d after transplantation	5	–	–

Effects of compound preparation of Cordyceps sinensis and Tripterygium hypoglaucum on survival time of pigskin after allogeneic transplantation

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