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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (5): 463-467.doi: 10.3736/jcim20090512

• Original Experimental Research • Previous Articles     Next Articles

Characteristics of sublingual vein and expressions of vascular endothelial growth factor and hypoxia-inducible factor 1α proteins in sublingual tissues of Beagle dogs with portal hypertension

Bai-yu Li, Li-na Wang, Xiao-qiang Yue, Bai Li   

  1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2009-03-03 Accepted:2009-03-20 Online:2009-05-20 Published:2009-05-15

Objective

To observe sublingual vein characteristics and the expressions of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1α (HIF-1α) proteins in sublingual tissues of Beagle dogs with cirrhotic portal hypertension.
Methods

Twelve Beagle dogs were randomly divided into normal control group and cirrhotic portal hypertension group. There were 6 dogs in each group. A canine model of cirrhosis portal hypertension was established by injecting dimethylnitrosamine (DMN) into portal vein once a week for 7 weeks. The characteristics of sublingual vein were observed. Portal venous pressure was measured by using bioelectric recording techniques. The expressions of VEGF and HIF-1α proteins in sublingual vein were detected by immunohistochemical method.
Results

The shape and color of sublingual vein in beagle dogs in the cirrhotic portal hypertension group changed obviously as compared with the normal control group. Immunohistochemical results showed that there were almost no expressions of VEGF and HIF-1α proteins in sublingual tissues in the normal control group; however, the expressions of VEGF and HIF-1α proteins in sublingual tissues in the cirrhotic portal hypertension group significantly increased.
Conclusion

Changes of portal pressure may lead to the formation of the abnormal sublingual vein by increasing the expressions of VEGF and HIF-1α proteins in sublingual tissues of Beagle dogs with portal hypertension.

Key words: Hypertension, portal, Vascular endothelial growth factors, Hypoxia-inducible factor la, Sublingual vein, Beagle dogs

Figure 1

Pathologic changes in liver tissues observed by HE staining (Light microscopy, ×200) A: Normal control group;B: Cirrhotic portal hypertension group."

Figure 2

Morphology of sublingual vein A: Normal control group; B: Cirrhotic portal hypertension group."

Table 1

Expressions of VEGF and HIF-1α proteins in sublingual tissues of Beagle dogs in different groups (x±s)"

Group n VEGF HIF-1α
Normal control 6 16.35±1.40 6.93±0.85
Cirrhotic portal hypertension 6 55.51±0.82** 187.10±7.14**

Figure 3

Expression of VEGF in sublingual tissues observed by immunohistochemical staining (Light microscopy, ×400) A: Normal control group; B: Cirrhotic portal hypertension group."

Figure 4

Expression of HIF-1α in sublingual tissues observed by immunohistochemical staining (Light microscopy, ×400) A: Normal control group; B: Cirrhotic portal hypertension group."

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