肝硬化门静脉高压模型犬舌下络脉形态和舌下组织血管内皮生长因子及低氧诱导因子1α的表达

doi:10.3736/jcim20090512

Abstract

Objective

To observe sublingual vein characteristics and the expressions of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1α (HIF-1α) proteins in sublingual tissues of Beagle dogs with cirrhotic portal hypertension.
Methods

Twelve Beagle dogs were randomly divided into normal control group and cirrhotic portal hypertension group. There were 6 dogs in each group. A canine model of cirrhosis portal hypertension was established by injecting dimethylnitrosamine (DMN) into portal vein once a week for 7 weeks. The characteristics of sublingual vein were observed. Portal venous pressure was measured by using bioelectric recording techniques. The expressions of VEGF and HIF-1α proteins in sublingual vein were detected by immunohistochemical method.
Results

The shape and color of sublingual vein in beagle dogs in the cirrhotic portal hypertension group changed obviously as compared with the normal control group. Immunohistochemical results showed that there were almost no expressions of VEGF and HIF-1α proteins in sublingual tissues in the normal control group; however, the expressions of VEGF and HIF-1α proteins in sublingual tissues in the cirrhotic portal hypertension group significantly increased.
Conclusion

Changes of portal pressure may lead to the formation of the abnormal sublingual vein by increasing the expressions of VEGF and HIF-1α proteins in sublingual tissues of Beagle dogs with portal hypertension.

Key words: Hypertension, portal, Vascular endothelial growth factors, Hypoxia-inducible factor la, Sublingual vein, Beagle dogs

Bai-yu Li, Li-na Wang, Xiao-qiang Yue, Bai Li. Characteristics of sublingual vein and expressions of vascular endothelial growth factor and hypoxia-inducible factor 1α proteins in sublingual tissues of Beagle dogs with portal hypertension. Journal of Chinese Integrative Medicine, 2009, 7(5): 463-467.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20090512

http://www.jcimjournal.com/EN/Y2009/V7/I5/463

TrendMD

Figures/Tables 5

Figure 1

Figure 2

Table 1

Figure 3

Figure 4

References 10

[1]	Chen Q, Xu ZW, Chai YQ . Research overview of sublingual vein diagnosis[J]. Xin Zhong Yi, 2003,35(3):70-71
	陈群, 徐志伟, 柴雅倩 . 舌下络脉诊法的研究概况[J]. 新中医, 2003,35(3):70-71
[2]	Liu Q, Yue XQ, Ren RZ, Ma CH, Ling CQ . Characteristics of sublingual venae in primary liver cancer patients in different clinical stages[J]. J Chin Integr Med, 2004,2(3):175-177
	刘庆, 岳小强, 任荣政, 马传红, 凌昌全 . 不同临床分期原发性肝癌患者的舌下络脉特征[J]. 中西医结合学报, 2004,2(3):175-177
[3]	Deng WZ, Gu W, Cheng BB, Han J, Yue XQ, Zhai XF, Ling CQ . Characteristics and formation mechanism of abnormal sublingual collaterals in rats with portal hypertension[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2007,13(6):430-432
	邓伟哲, 顾伟, 程彬彬, 韩洁, 岳小强, 翟笑枫, 凌昌全 . 门静脉高压症模型大鼠异常舌下络脉特点及形成机理的探讨[J]. 中国中医基础医学杂志, 2007,13(6):430-432
[4]	Kawasaki S, Umekita N, Beppu T, Wada T, Sugiyama Y, Iga T, Hanano M . Hepatic extraction of chenodeoxycholic acid in dogs chronically intoxicated with dimethylnitrosamine[J]. Biochem Pharmacol, 1984,33(20):3219-3224 doi: 10.1016/0006-2952(84)90080-7
[5]	Wu DB, Jin SY, Si ZX . Experimental study of hemodynamic changes of lingual vessels of rats with cirrhotic portal hypertension and its mechanism[J]. Zhonguo Bing Li Sheng Li Za Zhi, 1996,12(4):422-424
	吴定标, 靳士英, 司兆学 . 肝硬变门脉高压症大鼠舌脉的血流动力学变化及其发生机制的研究[J]. 中国病理生理杂志, 1996,12(4):422-424
[6]	Yue XQ, Gao JD, Zhai XF, Liu Q, Jiang D, Ling CQ . Correlation between the width of lingual vein and the changes of hemodynamics of portal system in patients with primary liver cancer[J]. J Chin Integr Med, 2006,4(5):482-484
	岳小强, 高静东, 翟笑枫, 刘庆, 蒋栋, 凌昌全 . 肝癌患者舌下络脉宽度与肝门脉系统血管及其血流动力学指标的相关性研究[J]. 中西医结合学报, 2006,4(5):482-484
[7]	Fernandez M, Vizzutti F, Garcia-Pagan JC, Rodes J, Bosch J . Anti-VEGF receptor-2 monoclonal antibody prevents portal-systemic collateral vessel formation in portal hypertensive mice[J]. Gastroenterology, 2004,126(3):886-894 doi: 10.1053/j.gastro.2003.12.012
[8]	Fernandez M, Mejias M, Angermayr B, Garcia-Pagan JC, Rodés J, Bosch J . Inhibition of VEGF receptor-2 decreases the development of hyperdynamic splanchnic circulation and portal-systemic collateral vessels in portal hypertensive rats[J]. J Hepatol, 2005,43(1):98-103 doi: 10.1016/j.jhep.2005.02.022
[9]	Moreau R . VEGF-induced angiogenesis drives collateral circulation in portal hypertension[J]. J Hepatol, 2005,43(1):6-8 doi: 10.1016/j.jhep.2005.04.002
[10]	Salceda S, Beck I, Caro J . Absolute requirement of aryl hydrocarbon receptor nuclear translocator protein for gene activation by hypoxia[J]. Arch Biochem Biophys, 1996,334(2):389-394 doi: 10.1006/abbi.1996.0469

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2]	Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3]	Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4]	Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5]	. Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6]	Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7]	Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8]	Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9]	Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10]	Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228

Group	n	VEGF	HIF-1α
Normal control	6	16.35±1.40	6.93±0.85
Cirrhotic portal hypertension	6	55.51±0.82**	187.10±7.14**

Characteristics of sublingual vein and expressions of vascular endothelial growth factor and hypoxia-inducible factor 1α proteins in sublingual tissues of Beagle dogs with portal hypertension

RichHTML

PDF (PC)

Like

Abstract

Cite this article

Share this article