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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (3): 299-305.doi: 10.3736/jcim20110311

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Jinmaitong-medicated serum on inducible nitric oxide synthase and NADPH oxidase p22-phox subunit of rat Schwann cells cultured in high-glucose medium

Zhao Li1,Liang Xiao-chun1(),Zhang Hong2,Wang Pu-yan1,Sun Qing1,Huang Wen-zhi1,Li Bo-wu1   

  1. 1. Department of Traditional Chinese Medicine,Peking Union Medical College Hospital, Chinese Academy of Medical Sciences,Beijing 100730, China
    2. Institute of Basic Medical Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, China
  • Received:2010-12-12 Accepted:2010-12-19 Online:2011-03-20 Published:2018-10-21
  • Contact: Liang Xiao-chun

Objective: To investigate the effects of medicated serum prepared by administration of Jinmaitong (JMT), a compound Chinese herbal medicine, on nicotinamide adenine dinucleotide phosphate (NADPH) oxidase p22-phox subunit and inducible nitric oxide synthase (iNOS) of rat Schwann cells cultured in high-glucose medium.
Methods: Wistar rats were divided into normal control group (distilled water), JMT (JMT at dose of 1.31 g/(kg·d)) group and vitamin C (vitamin C at dose of 0.08 g/(kg·d)) group to prepare medicated serum. Bilateral sciatic nerves of new born Wistar rats were used to separate Schwann cells. Schwann cells cultured in high-glucose medium were divided into high glucose group (cultured with 50 mmol/L glucose medium), JMT group (cultured with JMT-medicated serum) and vitamin C (VC) group (cultured with VC-medicated serum). Schwann cells cultured in DMEM were used as the normal control. After 48 h culturing, the expression of iNOS was detected by immunofluorescence method and p22-phox mRNA was tested by real-time fluorescent quantitative polymerase chain reaction.
Results: The expression levels of iNOS and p22-phox mRNA in the high glucose group were higher than those in the normal control group (P<0.01). Compared with the high glucose group, expressions of iNOS protein and p22-phox mRNA in JMT group were significantly decreased (P<0.01) and JMT-medicated serum had better effect than VC (P<0.01).
Conclusion: JMT-medicated serum can down-regulate the expressions of iNOS protein and NADPH oxidase p22-phox subunit mRNA of Schwann cells cultured in high-glucose medium.

Key words: high glucose, Schwann cells, inducible nitric oxide synthase, NADPH oxidase, drugs,Chinese herbal



Figure 1

Expression of iNOS in Schwann cells cultured in high-glucose medium tested by immunofluorescence method (Confocal laser scanning microscopy, ×400) Schwann cells were incubated with anti-inducible nitric oxide synthase (iNOS) and stained by fluorescein isothiocyanate (green); nuclei were counterstained by propidium iodide (red). A: Control group; B: High glucose (50 mmol/L) group; C: Jinmaitong-containing serum (10%) plus 50 mmol/L glucose group; D: Vitamin C-containing serum (20%) plus 50 mmol/L glucose group."

[1] Coppey LJ, Gellett JS, Davidson EP, Yorek MA . Preventing superoxide formation in epineurial arterioles of the sciatic nerve from diabetic rats restores endothelium-dependent vasodilation[J]. Free Radic Res, 2003,37(1):33-40
doi: 10.1080/1071576021000028442
[2] Vareniuk I, Pavlov IA, Obrosova IG . Inducible nitric oxide synthase gene deficiency counteracts multiple manifestations of peripheral neuropathy in a streptozotocin-induced mouse model of diabetes[J]. Diabetologia, 2008,51(11):2126-2133
doi: 10.1007/s00125-008-1136-3
[3] Yasuda H, Terada M, Maeda K, Kogawa S, Sanada M, Haneda M, Kashiwagi A, Kikkawa R . Diabetic neuropathy and nerve regeneration[J]. Prog Neurobiol, 2003,69(4):229-285
doi: 10.1016/S0301-0082(03)00034-0
[4] Eckersley L . Role of the Schwann cell in diabetic neuropathy[J]. Int Rev Neurobiol, 2002,50:293-321
doi: 10.1016/S0074-7742(02)50081-7
[5] Liang XC, Cui LY, Guo SS, Zhang KJ, Hao WX, Jia L, Zhang H, Sun RY, Li BH, Du HL . Clinical study on Jinmaitong Composita on diabetic peripheral neuropathy[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 1999,19(9):517-519
梁晓春, 崔丽英, 郭赛珊, 张克俭, 郝炜欣, 贾力, 张宏, 孙仁宇, 李本红, 杜红 . 筋脉通治疗糖尿病周围神经病变的临床观察[J]. 中国中西医结合杂志, 1999,19(9):517-519
[6] Hao WX, Jia L, Xu HY, Zhang H, Guo SS, Liang XC . Effect of Jinmaitong mixture on conduction velocity of sciatic nerve and antioxidant level of erythrocyte in diabetic rats[J]. Zhongguo Xin Yao Za Zhi, 2003,12(5):343-345
郝伟欣, 贾力, 徐惠媛, 张宏, 郭赛珊, 梁晓春 . 筋脉通对大鼠坐骨神经传导速度及红细胞抗氧化作用的影响[J]. 中国新药杂志, 2003,12(5):343-345
[7] Qu L, Liang XC, Zhang H, Wu QL, Sun LQ, Gu B . Effect of Jinmaitong serum on the proliferation of rat Schwann cells cultured in high glucose medium[J]. Chin J Integr Med, 2008,14(4):293-297
doi: 10.1007/s11655-008-0293-z
[8] Edwards JL, Vincent AM, Cheng HT, Feldman EL . Diabetic neuropathy: mechanisms to management[J]. Pharmacol Ther, 2008,120(1):1-34
doi: 10.1016/j.pharmthera.2008.05.005 pmid: 18616962
[9] Brownlee M . Biochemistry and molecular cell biology of diabetic complications[J]. Nature, 2001,414(6865):813-820
doi: 10.1038/414813a
[10] Guzik TJ, Mussa S, Gastaldi D, Sadowski J, Ratnatunga C, Pillai R, Channon KM . Mechanisms of increased vascular superoxide production in human diabetes mellitus: role of NAD(P)H oxidase and endothelial nitric oxide synthase[J]. Circulation, 2002,105(14):1656-1662
doi: 10.1161/01.CIR.0000012748.58444.08
[11] Hink U, Li H, Mollnau H, Oelze M, Matheis E, Hartmann M, Skatchkov M, Thaiss F, Stahl RA, Warnholtz A, Meinertz T, Griendling K, Harrison DG, Forstermann U, Munzel T . Mechanisms underlying endothelial dysfunction in diabetes mellitus[J]. Circ Res, 2001,88(2):14-22
[12] Cahilly C, Ballantyne CM, Lim DS, Gotto A, Marian AJ . A variant of p22(phox), involved in generation of reactive oxygen species in the vessel wall, is associated with progression of coronary atherosclerosis[J]. Circ Res, 2000,86(4):391-395
doi: 10.1161/01.RES.86.4.391
[13] Inoguchi T, Li P, Umeda F, Yu HY, Kakimoto M, Imamura M, Aoki T, Etoh T, Hashimoto T, Naruse M, Sano H, Utsumi H, Nawata H . High glucose level and free fatty acid stimulate reactive oxygen species production through protein kinase C-dependent activation of NAD(P)H oxidase in cultured vascular cells[J]. Diabetes, 2000,49(11):1939-1945
doi: 10.2337/diabetes.49.11.1939
[14] Vincent AM, Perrone L, Sullivan KA, Backus C, Sastry AM, Lastoskie C, Feldman EL . Receptor for advanced glycation end products activation injures primary sensory neurons via oxidative stress[J]. Endocrinology, 2007,148(2):548-558
doi: 10.1210/en.2006-0073
[15] San Martin A, Foncea R, Laurindo FR, Ebensperger R, Griendling KK, Leighton F . Nox1-based NADPH oxidase-derived superoxide is required for VSMC activation by advanced glycation end-products[J]. Free Radic Biol Med, 2007,42(11):1671-1679
doi: 10.1016/j.freeradbiomed.2007.02.002
[16] Zochodne DW, Verge VM, Cheng C, Hke A, Jolley C, Thomsen K, Rubin I, Lauritzen M .Nitric oxide synthase activity and expression in experimental diabetic neuropathy[J]. J Neuropathol Exp Neurol, 2000,59(9):798-807
doi: 10.1093/jnen/59.9.798
[17] Olukman M, Orhan CE, Celenk FG, Ulker S . Apocynin restores endothelial dysfunction in streptozotocin diabetic rats through regulation of nitric oxide synthase and NADPH oxidase expressions[J]. J Diabetes Complications, 2010,24(6):415-423
doi: 10.1016/j.jdiacomp.2010.02.001
[18] Liang XC, Tang DQ, Guo SS, Zhang JW, Sun RY, Zhang H, Ling YL . Effect of Wenjingtong Composita on mRNA expression of receptor of the advanced glycation end products in sciatic nerve of streptozotocin-induced diabetic rats[J]. Zhongguo Tang Niao Bing Za Zhi, 2002,10(4):219-221
doi: 10.3321/j.issn:1006-6187.2002.04.008
梁晓春, 唐代屹, 郭赛珊, 张建伟, 孙仁宇, 张宏, 凌允礼 . 温筋通对链脲佐菌素糖尿病大鼠坐骨神经糖化终产物受体mRNA表达的调节[J]. 中国糖尿病杂志, 2002,10(4):219-221
doi: 10.3321/j.issn:1006-6187.2002.04.008
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