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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (3): 299-305.doi: 10.3736/jcim20110311

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Jinmaitong-medicated serum on inducible nitric oxide synthase and NADPH oxidase p22-phox subunit of rat Schwann cells cultured in high-glucose medium

Zhao Li1,Liang Xiao-chun1(),Zhang Hong2,Wang Pu-yan1,Sun Qing1,Huang Wen-zhi1,Li Bo-wu1   

  1. 1. Department of Traditional Chinese Medicine,Peking Union Medical College Hospital, Chinese Academy of Medical Sciences,Beijing 100730, China
    2. Institute of Basic Medical Science, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, China
  • Received:2010-12-12 Accepted:2010-12-19 Online:2011-03-20 Published:2018-10-21
  • Contact: Liang Xiao-chun E-mail:xcliang@vip.sina.com

Objective: To investigate the effects of medicated serum prepared by administration of Jinmaitong (JMT), a compound Chinese herbal medicine, on nicotinamide adenine dinucleotide phosphate (NADPH) oxidase p22-phox subunit and inducible nitric oxide synthase (iNOS) of rat Schwann cells cultured in high-glucose medium.
Methods: Wistar rats were divided into normal control group (distilled water), JMT (JMT at dose of 1.31 g/(kg·d)) group and vitamin C (vitamin C at dose of 0.08 g/(kg·d)) group to prepare medicated serum. Bilateral sciatic nerves of new born Wistar rats were used to separate Schwann cells. Schwann cells cultured in high-glucose medium were divided into high glucose group (cultured with 50 mmol/L glucose medium), JMT group (cultured with JMT-medicated serum) and vitamin C (VC) group (cultured with VC-medicated serum). Schwann cells cultured in DMEM were used as the normal control. After 48 h culturing, the expression of iNOS was detected by immunofluorescence method and p22-phox mRNA was tested by real-time fluorescent quantitative polymerase chain reaction.
Results: The expression levels of iNOS and p22-phox mRNA in the high glucose group were higher than those in the normal control group (P<0.01). Compared with the high glucose group, expressions of iNOS protein and p22-phox mRNA in JMT group were significantly decreased (P<0.01) and JMT-medicated serum had better effect than VC (P<0.01).
Conclusion: JMT-medicated serum can down-regulate the expressions of iNOS protein and NADPH oxidase p22-phox subunit mRNA of Schwann cells cultured in high-glucose medium.

Key words: high glucose, Schwann cells, inducible nitric oxide synthase, NADPH oxidase, drugs,Chinese herbal

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Figure 1

Expression of iNOS in Schwann cells cultured in high-glucose medium tested by immunofluorescence method (Confocal laser scanning microscopy, ×400) Schwann cells were incubated with anti-inducible nitric oxide synthase (iNOS) and stained by fluorescein isothiocyanate (green); nuclei were counterstained by propidium iodide (red). A: Control group; B: High glucose (50 mmol/L) group; C: Jinmaitong-containing serum (10%) plus 50 mmol/L glucose group; D: Vitamin C-containing serum (20%) plus 50 mmol/L glucose group."

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