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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (9): 877-882.doi: 10.3736/jcim20100912

• Original Experimental Research • Previous Articles     Next Articles

Effects of serum containing Fructus Cnidii and Psoralea corylifolia on highly metastatic human breast cancer cell line MDA-MB-231BO and bone marrow stromal cell line ST-2

 Sheng Liua, Xiao-yun Songa, Chun-yu Wua, Xiang-hui Hana, Ling-lin Liua,Jian-wen Liub,Yi-chun Xub   

  1. a Department of Chinese Traditional Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    b State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
  • Received:2010-03-05 Accepted:2010-05-05 Online:2010-09-20 Published:2010-09-15

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Objective

To explore the effects of different proportions of Fructus Cnidii (Shechuangzi) and Psoralea corylifolia (Buguzhi) on highly metastatic human breast cancer cell line MDA-MB-231BO and bone marrow stromal cell line ST-2 in vitro.
Methods

Thirty-six female SD rats were randomly divided into 6 groups to prepare the drug-medicated sera by administering with different proportions of Fructus Cnidii and Psoralea corylifolia, including 4︰0 group, 3︰1 group, 1︰1 group, 1︰3 group, 0︰4 group and control group. MDA-MB-231BO cells and ST-2 cells were cultured in Dulbecco’s modified Eagle’s medium containing drug-medicated serum. Inhibition rates of MDA-MB-231BO cells and ST-2 cells were measured by methyl thiazolyl tetrazolium (MTT) method; migration ability of MDA-MB-231BO cells was tested by a cell migration experiment; alkaline phosphatase activity (ALP) of ST-2 cells was measured by using 4-nitrophenyl phosphate disodium salt, and mineralized nodule formation of ST-2 cells was measured by alizarin red staining.
Results

Sera contaning different proportions of Fructus Cnidii and Psoralea corylifolia inhibited the migration activity of MDA-MB-231BO cells as compared with the blank serum, and serum contaning Fructus Cnidii and Psoralea Corylifolia at proportion of 1︰1 had the best function (P<0.01). Fructus Cnidii and Psoralea corylifolia at ratio of 1︰1 also enhanced the ALP activity of ST2 cells (P<0.05) and increased the number of mineralized nodules of ST2 cells (P<0.01).
Conclusion

Kidney-warming recipe of Fructus Cnidii and Psoralea corylifolia can inhibit proliferation and migration of MDA-MB-231BO cells and increase the activity of ST-2 cells.

Key words: Breast neoplasms, MDA-MB-231BO cell, ST-2 cell, Fructus Cnidii, Psoralea corylifolia L

Figure 1

Inhibition rates of MDA-MB-231BO cells by sera containing different proportions of Fructus Cnidii and Psoralea corylifolia Data were represented as x±s, n=3. *P<0.05, vs control group."

Figure 2

Inhibition rates of ST-2 cells by sera containing different proportions of Fructus Cnidii and Psoralea corylifolia Data were represented as x±s, n=3. *P<0.05, vs control group."

Figure 3

Effects of sera containing different proportions of Fructus Cnidii and Psoralea corylifolia on migration of MDA-MB-231BO cells Data were represented as x±s, n=3. *P<0.05, **P<0.01, vs control group."

Figure 4

Effects of sera containing different proportions of Fructus Cnidii and Psoralea corylifolia on ALP activity of ST-2 cells Data were represented as x±s, n=3. *P<0.05, vs control group."

Figure 5

Effects of sera containing different proportions of Fructus Cnidii and Psoralea corylifolia on mineralized nodule formation of ST-2 cells Data were represented as x±s, n=3. *P<0.05, **P<0.01, vs control group."

Figure 6

Effects of sera containing different proportions of Fructus Cnidii and Psoralea corylifolia on mineralized nodule formation of ST-2 cells (Inverted biological microscopy, alizarin red staining, ×200) A: Control group; B: 4︰0 group; C: 3︰1 group; D: 1︰1 group; E: 1︰3 group; F: 0︰4 group."

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