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Journal of Integrative Medicine ›› 2016, Vol. 14 ›› Issue (1): 51-59.doi: 10.1016/S2095-4964(16)60238-8

• Research Article • Previous Articles     Next Articles

Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells

Qing-qingZanga, LuZhanga, NingGaob, ChengHuanga   

  1. a Drug Discovery Laboratory, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    b College of Pharmacy, Third Military Medical University, Chongqing 400038, China
  • Received:2015-03-30 Accepted:2015-06-26 Online:2016-01-15 Published:2016-01-15
  • Contact: Cheng Huang, PhD, Professor; Tel: +86-21-51322182; E-mail: chuang@shutcm.edu.cn. Ning Gao; E-mail: gaoning59@aliyun.com

Objective

To investigate the effects of ophiopogonin D on human breast cancer MCF-7 cells.

Methods

Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation experiments. Cell cycle was measured with cell cycle flow cytometry and a living cell assay. Apoptosis and terminal deoxynucleoitidyl transferase-mediated dUTP nick end labeling assays were performed to detect the apoptosis of MCF-7 cells induced by ophiopogonin D. Finally, Western blotting was used to explore the mechanism.

Results

Exposure of cells to ophiopogonin D resulted in marked decreases in viable cells and colony formation with a dose-dependent manner. Treatment of these cells with ophiopogonin D also resulted in cell cycle arrest at the G2/M phase, and increased apoptosis. Mechanistically, ophiopogonin D-induced G2/M cell cycle arrest was associated with down-regulation of cyclin B1. Furthermore, activation of caspase-8 and caspase-9 was involved in ophiopogonin D-induced apoptosis.

Conclusion

The data suggested that ophiopogonin D inhibits MCF-7 cell growth via the induction of cell cycle arrest at the G2/M phase.

Key words: Ophiopogonin D, G2/M arrest, Apoptosis, Cyclin B1, Caspases, Breast neoplasms, Antineoplastic agents, Phytogenic, In vitro

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