Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells

doi:10.1016/S2095-4964(16)60238-8

Abstract

Objective

To investigate the effects of ophiopogonin D on human breast cancer MCF-7 cells.

Methods

Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation experiments. Cell cycle was measured with cell cycle flow cytometry and a living cell assay. Apoptosis and terminal deoxynucleoitidyl transferase-mediated dUTP nick end labeling assays were performed to detect the apoptosis of MCF-7 cells induced by ophiopogonin D. Finally, Western blotting was used to explore the mechanism.

Results

Exposure of cells to ophiopogonin D resulted in marked decreases in viable cells and colony formation with a dose-dependent manner. Treatment of these cells with ophiopogonin D also resulted in cell cycle arrest at the G₂/M phase, and increased apoptosis. Mechanistically, ophiopogonin D-induced G₂/M cell cycle arrest was associated with down-regulation of cyclin B1. Furthermore, activation of caspase-8 and caspase-9 was involved in ophiopogonin D-induced apoptosis.

Conclusion

The data suggested that ophiopogonin D inhibits MCF-7 cell growth via the induction of cell cycle arrest at the G₂/M phase.

Key words: Ophiopogonin D, G₂/M arrest, Apoptosis, Cyclin B1, Caspases, Breast neoplasms, Antineoplastic agents, Phytogenic, In vitro

Qing-qing Zang, Lu Zhang, Ning Gao, Cheng Huang. Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G₂/M, and induces apoptosis in human breast carcinoma MCF-7 cells. Journal of Integrative Medicine, 2016, 14(1): 51-59.

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Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G₂/M, and induces apoptosis in human breast carcinoma MCF-7 cells

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