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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (2): 181-185.doi: 10.3736/jcim20100214

• Original Experimental Research • Previous Articles     Next Articles

Effects of Huangqi Guizhi Wuwu Decoction given by different administration methods on rats with frostbite and the mechanism

Yan-yan Lia, Yong-hui Wanga, Yong-gang Zhangb, Yun Baia, Ran Zhoua   

  1. a Department of Herbal Formula Science,School of Basic Sciences, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, Shanxi Province, China
    b Department of Chinese Materia Medica, Pharmacy College of Shanxi Medical University, Taiyuan 030024, Shanxi Province, China
  • Received:2009-04-20 Accepted:2009-12-18 Online:2010-02-20 Published:2010-02-15
  • Contact: Yan-yan Li E-mail:liyy1002@163.com

Objective

To establish a rat model of frostbite and to evaluate the effects of different administration methods of Huangqi Guizhi Wuwu Decoction (HGWD), a compound traditional Chinese herbal medicine for warming meridians to disperse cold, on rats with frostbite.
Methods

Frostbite in rats was induced by the method of soaking feet in hypothermia ethanol-water mixture. Levels of interleukin-6 (IL-6), tumor necrosis factor-β (TNF-β), thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) in serum of rats treated with different administration methods of HGWD, such as oral administration (Oral HGWD), soak (Soak HGWD), and oral administration plus soak (Oral-soak HGWD), were tested by enzyme-linked immunosorbent assay.
Results

IL-6, TNF-β, TXB2 levels were significantly higher (P<0.01) and 6-keto-PGF1α level was lower (P<0.01) in serum of rats in the untreated group than in the normal control group. Compared with the untreated group, the level of IL-6 obviously decreased (P<0.05) in serum of the rats treated by oral HGWD, while no significant decrease (P>0.05) was observed in the soak HGWD group, and there was no interaction (P>0.05) between the two administration methods in regulating the level of IL-6. The levels of TNF-β obviously decreased (P<0.01, P<0.05) in serum of the rats treated by oral and soak HGWD, and there was interaction between the two administration methods. The level of TNF-β in the oral HGWD group was significantly lower than that in the soak HGWD group (P<0.01). Compared with the untreated group, level of TXB2 in oral HGWD or soak HGWD group did not decrease significantly (P>0.05) and there was no interaction (P>0.05) between the two administration methods. The level of 6-keto-PGF1α was obviously increased (P<0.01) in serum of the rats treated by oral HGWD, while there was no significant decrease (P>0.05) in the soak HGWD group as compared with the untreated group, and there was interaction (P<0.05) between the two administration methods in regulating the level of 6-keto-PGF1α.
Conclusion

Rats with frostbite has immunologic dysfunction and a state of forming thrombus easily. The oral-soak HGWD can improve frostbite of local skin in rats. The therapeutic mechanism of HGWD may be to regulate the dysfunction of immune system and the imbalance of TXB2-PGF1α.

Key words: Huangqi Guizhi Wuwu Decoction, Chilblains' disease models, AnimaU administration, OraU administration, TopicaU rats

Figure 1

The frostbite foot of model rat on 1 day after creation of models"

Table 1

Effects of HGWD given by different administration methods on serum content of IL-6 in frostbite rats ($\bar{x}±s$, ng/L, n=10)"

Oral Soak F P
A No C Yes
B No 208.25±47.49 178.28±29.26 6.34a <0.05a
D Yes 161.50±34.53 136.00±19.16
F 2.89a F=0.043b
P >0.05a P>0.05b

Table 2

Effects of HGWD given by different administration methods on serum content of TNF-β in frostbite rats ($\bar{x}±s$, ng/L, n=10)"

Oral Soak F P
A No C Yes
B No 13.88±3.91 9.92±2.40 75.00a
<0.01a
D Yes 2.85±0.98 2.60±0.58
F 7.48a F=6.21b
P <0.05a P<0.05b

Table 3

Effects of HGWD given by different administration methods on serum content of TXB2 in frostbite rats ( $\bar{x}±s$, ng/L, n=10)"

Oral Soak F P
A No C Yes
B No 241.20±43.07 232.45±36.70 4.15a
>0.05a
D Yes 212.78±9.46 172.70±39.87
F 0.24a F=2.01b
P >0.05a P>0.05b

Table 4

Effects of HGWD given ty different administration methods on serum content of 6-keto-PGF1α in frostbite rats ($\bar{x}±s$, ng/L, n=10)"

Oral Soak F P
A No C Yes
B No 2.69±0.19 2.92±0.35 11.88a
<0.01a
D Yes 3.38±0.60 4.41±0.84
F 3.58a F=5.20b
P >0.05a P<0.05b
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