苍耳子中酚酸类化合物及不同品种和居群苍耳子中总酚酸含量的测定

doi:10.3736/jcim20060217

Abstract

Objective

To study the chemical constituents of Fructus Xanthii and to determine the contents of total phenolic acids (TPA) in fruits of Xanthium from different populations for evaluating the quality of them.

Methods

Components in Fructus Xanthii were isolated and purified by various column chromatographies and the contents of TPA were determined by ultraviolet spectrophotometry with chlorogenic acid (CHA) as reference substance.

Results

Six caffeoylquinic acids along with caffeic acid and ferulic acid were isolated from Fructus Xanthii. The contents of TPA of the samples collected from 29 populations in China varied from 0.31% to 1.44%. Among the samples originated from two species and 1 variety of Xanthium, the contents of TPA in X. sibiricum var. subinerme samples with an average of 0.36% were relatively lower than those in other 2 species. While the content of TPA in Sample 3 collected from Shanghai was 1.44% and the highest among all the samples, and that in Sample 12 from Xinjian of Jiangxi Province was 0.38% and the lowest among the X. sibiricum samples.

Conclusion

5-O-caffeoylquinic acid, 1,4-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid were isolated from Xanthium plant for the first time. The difference of contents of TPA in samples from different species and different populations in China was relatively significant. Fructus Xanthii in Shanghai and Sanming of Fujian Province were considered high quality if contents of TPA were used as reference for quality evaluating.

Key words: Fructus Xanthii, Phenolic acid, Caffeoylquinic acid, Ultraviolet spectrophotometry, Chemical structure identification, Population

CLC Number:

R284.1

Ting Han, Hui-liang Li, Yuan Hu, Qiao-yan Zhang, Bao-kang Huang, Han-chen Zheng, Khalid Rahman, Lu-ping Qin. Phenolic acids in Fructus Xanthii and determination of contents of total phenolic acids in different species and populations of Xanthium in China. Journal of Chinese Integrative Medicine, 2006, 4(2): 194-198.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20060217

http://www.jcimjournal.com/EN/Y2006/V4/I2/194

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Sample No.	Latin name	Collected location	Collected date (y.m.d)	TPA (%)
1	X.sibiricum var. subinerme	Renqiu, Hebei Province	2003.11.13	0.31
2	X. sibiricum	Jinan, Shandong Province	2003.11.3	0.68
3	X. sibiricum	Sunqiao, Shanghai	2003.11.4	1.44
4	X. sibiricum	Bengbu, Anhui Province	2003.11.23	0.55
5	X. sibiricum	Hangzhou, Zhejiang Province	2003.11.11	0.94
6	X. sibiricum	Lianyungang, Jiangsu Province	2003.11.13	0.66
7	X. mongolicum	Yantai, Shandong Province	2003.11.7	0.51
8	X. sibiricum	Kaili, Guizhou Province	2004.7.30	0.40
9	X. mongolicum	Guilin, Guangxi Autonomous Region	2003.11.18	0.62
10	X. sibiricum	Ding’an, Hainan Province	2004.2.7	0.87
11	X. sibiricum	Huangshan, Anhui Province	2003.11.30	0.59
12	X. sibiricum	Xinjian, Jiangxi Province	2003.11.25	0.38
13	X. sibiricum	Gaoan, Jiangxi Province	2003.12.9	0.59
14	Unknown	Shenzhen, Guangdong Province	2003.11.17	0.41
15	X. sibiricum	Tianmen, Hubei Province	2004.2.16	0.58
16	X. sibiricum	Zhumadian, Henan Province	2003.11.7	0.75
17	X. sibiricum	Nanjing, Jiangsu Province	2003.10.31	0.40
18	X. sibiricum	Linyi, Shandong Province	2003.11.7	0.45
19	X. sibiricum	Miluo, Hunan Province	2003.12.11	0.52
20	X. sibiricum	Quzhou, Zhejiang Province	2004.1.9	0.45
21	X. mongolicum	Changsha, Hunan Province	2003.12.15	0.59
22	X.sibiricum var. subinerme	Chifeng, the Inner Mongolia Autonomous Region	2003.10.28	0.43
23	X. sibiricum	Kunming, Yunnan Province	2003.12.1	0.54
24	X. sibiricum	Qingdao, Shandong Province	2003.11.8	0.65
25	X.sibiricum var. subinerme	Beijing	2003.11.20	0.34
26	X. sibiricum	Xiangyin, Hunan Province	2003.11.20	0.47
27	X. sibiricum	Sanming, Fujian Province	2003.11.8	1.41
28	X. sibiricum	Quanzhou, Fujian Province	2004.8.7	0.58
29	X.sibiricum var. subinerme	Xi'an, Shaanxi Province	2003.11.4	0.36

No.	Name of compound	R1	R2	R3	R4	R5
Compound 1	3-O-caffeoylquinic acid, chlorogenic acid	H	H	Caffeoyl	H	H
Compound 2	5-O-caffeoylquinic acid	H	H	H	H	Caffeoyl
Compound 3	1,5-di-O-caffeoylquinic acid	H	Caffeoyl	H	H	Caffeoyl
Compound 4	1,4-di-O-caffeoylquinic acid	H	Caffeoyl	H	Caffeoyl	H
Compound 5	4,5-di-O-caffeoylquinic acid	H	H	H	Caffeoyl	Caffeoyl
Compound 6	1,3,5-tri-O-caffeoylquinic acid	Caffeoyl	H	Caffeoyl	HCaffeoyl
Compound 7	Caffeic acid
Compound 8	Ferulic acid

Added (mg)	Found (mg)	Recovery (%)	Mean (%)	RSD (%)
1.10	1.08	98.2
1.08	1.06	98.1
1.17	1.20	102.5	99.78	1.89
1.06	1.07	100.9
1.18	1.17	99.2

Phenolic acids in Fructus Xanthii and determination of contents of total phenolic acids in different species and populations of Xanthium in China

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