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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 517-520.doi: 10.3736/jcim20070508

• Original Clinical Research • Previous Articles     Next Articles

Effects of three compounds extracted from Tripterygium wilfordii Hook on angiogenesis in chick chorioallantoic membrane

Guo-fu Zhu1, Hui-qing Zhang2, Ai-jun Hou3, Yan-long Yang2, Qiu-shuang Li1, Cong-cong Zhang1   

  1. 1. Teaching and Research Group of Chinese Materia Madica , School of Chinese Materia Madica , Shanghai University of Traditional Chinese Medicine , Shanghai 201203 ,China
    2. School of Traditional Chinese Medicine , Second Military Medical University , Shanghai 200433, China
    3. Teaching and Research Group of Pharmacognosy , School of Pharmacy , Fudan University , Shangha 200032, China
  • Online:2007-09-20 Published:2007-09-15

Objective: To investigate the effects of three compounds extracted from Tripterygium wilfordii Hook (TW) on angiogenesis in the chick chorioallantoic membrane (CAM).Methods: Fifty fresh Hongkong Mahua chicken eggs were divided into five groups: PBS-treated group, TW1-, TW2- and TW3-treated groups and Rg3-treated group. After disinfection, the eggs were incubated for six days in a constant temperature box with the temperature being controlled within 37.8 ℃, then exposed CAM, laid the filter papers with specimen on the CAM, and the eggs were incubated for another two days. CAM was fixed with the mixture of methyl alcohol and acetone at room temperature for about 15 min, and then cutting the CAM, taking photos and observing the angiogenesis in the CAM.Results: There were many CAM vessels in the PBS-treated group and the blood vessel net could be seen clearly. The number of CAM vessels in the TW1-, TW2- and TW3-treated groups (10 μg/egg) was much less than that in the PBS-treated group. Furthermore, the frame of the vessels was not clear, and the color was obscure. Inhibition rates of angiogenesis in the TW1-, TW2- and TW3-treated groups were 80%, 60% and 100% respectively, while the inhibition rate of angiogenesis in the Rg3-treated group (10 μg/egg) was only 10%.Conclusion: >TW1, TW2 and TW3 can obviously restrain the angiogenesis in CAM and still need further study.

CLC Number: 

  • R917.77

Table 1

Effects of TW1, TW2 and TW3 on angiogenesis of CAM"

Group n Dose (μg/egg) Result Inhibition rate (%)
+ ++
PBS-treated 10 10 0 0
TW1-treated 10 10 2 3 5 80
TW2-treated 10 10 4 4 2 60
TW3-treated 10 10 0 1 9 100
Rg3-treated 10 10 9 1 0 10

Figure 1

Effects of TW1, TW2 and TW3 on angiogenesis in CAMA: PBS-treated group; B: TW1-treated group; C: TW2-treated group; D: TW3-treated group; E: Rg3-treated group."

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