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Journal of Integrative Medicine ›› 2019, Vol. 17 ›› Issue (5): 366-373.

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Cyclophosphamide-induced reproductive toxicity: Beneficial effects of Helichrysum odoratissimum (Asteraceae) in male Wistar rats

Pierre Watchoa(), Ismaelle Rosine Mpecka, Patrick Brice Deeh Defoa, Modeste Wankeu-Nyab, Esther Ngadjuia, Georges Romeo Bonsou Fozina, Pierre Kamtchouingc, Albert Kamanyia   

  1. a. Animal Physiology and Phytopharmacology Laboratory, University of Dschang, P.O. BOX 67, Dschang, Cameroon
    b. Department of Animal Organisms Biology, University of Douala, P.O. BOX 24157, Douala, Cameroon
    c. Department of Animal Biology and Physiology, University of Yaoundé, P.O. BOX 812, Yaoundé, Cameroon
  • Received:2018-08-18 Online:2019-09-06 Published:2019-09-10

Objective
Cyclophosphamide (CP) is commonly used to treat some cancers, but its clinical efficacy is also linked with testicular toxicity. We investigated the effects of aqueous extract (AE) and methanolic extract (ME) of Helichrysum odoratissimum for reducing CP-induced reproductive toxicity in male rats.

Methods
In addition to a normal control (group 1), drugs or vehicles were administered orally to seven groups (n?=?5) of rats that had already received 4-weeks of pre-treatment with CP (5?mg/[kg·d], per oral administration); group 2 received CP?+?distilled water (10?mL/[kg·d]); group 3 received CP?+?5% tween 80 (10?mL/[kg·d]); group 4 received CP?+?clomiphene citrate (0.25?mg/[kg·d]); groups 5 and 6 received CP?+?AE (50 and 100?mg/[kg·d]) and groups 7 and 8 received CP?+?ME (50 and 100?mg/[kg·d]). Animals were sacrificed on day 15, and body and sexual organ weights, sperm characteristics, testosterone level and testicular histology were evaluated.

Results
The CP-treated group showed a significant reduction (P?<?0.001) in the body and seminal vesicle weights, testosterone level, sperm count, sperm motility and sperm viability, but elevated (P?<?0.001) sperm morphological abnormalities and testicular structure alterations, compared to the control group. Interestingly, these detrimental effects of CP were reversed by treatment with H. odoratissimum extracts. For instance, both extracts and all doses of H. odoratissimum significantly increased the sperm count (P?<?0.001), sperm motility (AE, 50?mg/kg, P?<?0.05; ME, 50 and 100?mg/kg, P?<?0.05) and sperm viability (AE, 50?mg/kg, P?<?0.001; ME, 50 and 100?mg/kg, P?<?0.001) compared to the CP group. H. odoratissimum also improved plasmatic and intratesticular testosterone levels and prevented histological alterations of the testes.

Conclusion
H. odoratissimum might be considered as an alternative drug to alleviate/prevent reproductive damage in cancer patients receiving CP chemotherapy.

Key words: Helichrysum odoratissimum, Cyclophosphamide, Reproductive toxicity, Rat

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