Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (4): 307-309.doi: 10.3736/jcim20050417

• Original Experimental Research • Previous Articles     Next Articles

Effect of Xingding Injection on expression of heat shock protein 72 in vascular endothelial cells

Jing Li1,Yun-Xia Wu2,Mei-chun Zhang3   

  1. 1. Department of Emergency, Hubei General Hospital of Chinese People’s Armed Police Forces, Wuhan, Hubei Province 430060, China;
    2. Institute of Integrated Traditional Chinese and Western Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province 430030, China
    3. Department of Internal Medicine, Hubei General Hospital of Chinese People’s Armed Police Forces, Wuhan, Hubei Province 430060, China;
  • Received:2004-06-06 Online:2005-07-20 Published:2005-07-20
  • Contact: Yun-xia Wu E-mail:wyx7419@yahoo.com.cn

Objective

To study the effect of Xingding Injection on the expression of heat shock protein 72 (HSP 72) in vascular endothelial cells after ultraviolet radiation.

Methods

Porcine aortic endothelial cells were cultured for 72 hours in culture mediums with different concentrations (0.1, 0.5, 1.0, 5.0, 10 mg/ml) of Xingding Injection. Ultraviolet radiation was administered to the cultured cells for 30 minutes. Western-blot assay was used to measure the expression of HSP 72 in the vascular endothelial cells.

Results

There was no expression of HSP 72 in the cultured vascular endothelial cells without ultraviolet radiation, but there was some expression of HSP 72 after ultraviolet radiation. Xingding Injection of different concentrations could significantly improve the expression of HSP 72. The expression of HSP 72 in the vascular endothelial cells cultured in culture medium with 1.0 mg/ml Xingding Injection was the highest, and there was no more increase of expression when the concentration was higher, instead the expression decreased.

Conclusion

Xingding Injection can protect the vascular endothelial cells from injury during stress. It may be one of its mechanisms in preventing and treating cardio-cerebrovascular disorders.

Key words: Xingding Injection, Vascular endothelial cells, Heat shock protein

CLC Number: 

  • R285.5

Fig 1

Configuration of aortic endothelial cells (×100)"

Fig 2

Positive expression of factor Ⅷ-related antigen of endothelial cells (×400)"

"

Culture medium n Expression of
HSP 72 (OD)
Normal control 5 50.38±2.33
Xingding Injection (0.1 mg/ml) 5 116.82±3.58*
Xingding Injection (0.5 mg/ml) 5 159.19±6.31**
Xingding Injection (1.0 mg/ml) 5 184.86±4.34**
Xingding Injection (5.0 mg/ml) 5 81.43±2.08
Xingding Injection (1 0 mg/ml) 5 60.18±1.67
[1] Ranson NA, White HE, Sailbil HR . Chaperonins[J]. Biochem J, 1998,333(Pt 2):233-242
doi: 10.1042/bj3330233
[2] Gerge J, Shoenfeld Y, Georgopoulos C . Heat shock proteins and stress tolerance in the biology of heat shock proteins and molecular chaperones[J]. Arteri Thromb Vasc Biol, 1999,19(3):505-510
doi: 10.1161/01.ATV.19.3.505 pmid: 10073950
[3] Angelidis CE, Lazaridis I, Pagoulatos GN . Constitutive expression of heat-shock protein 70 in mammalian cells confers thermoresistance[J]. Eur J Biochem, 1991,199(1):35-39
doi: 10.1111/j.1432-1033.1991.tb16088.x pmid: 1712300
[4] Rajalingam R, Mehra NK, Singal DP . Polymorphism in heat-shock protein 70-1(HSP 70-1) gene promoter region and susceptibility to tubercloid leprosy and pulmonary tuberculosis in Asian Indians[J]. Indian J Exp Biol, 2000,38(7):658-662
[5] Slater DN, Sloan JM . The porcine endothelial cell in tissue culture[J]. Atherosclerosis, 1975,21(2):259-272
doi: 10.1016/0021-9150(75)90085-4 pmid: 165826
[6] Basta G, Venneri L, Lazzerini G , et al. In vitro modulation of intracellular oxidative stress of endothelial cells by diagnostic cardiac ultrasound[J]. Cardiovasc Res, 2003,58(1):156-161
doi: 10.1016/S0008-6363(02)00665-X
[7] Yoshida H, Haze K, Yanagi H , et al. Identification of the cis-acting endoplasmic reticulum stress response element responsible for transcriptional induction of mammalian glucose-regulated proteins. Involvement of basic leucine zipper transcription factors[J]. J Biol Chem, 1998,273(50):33741-33749
doi: 10.1074/jbc.273.50.33741
[8] Chu B, Soncin F, Price BD , et al. Sequential phosphorylation by mitogen-activated protein kinase and glycogen synthase kinase 3 represses transcriptional activation by heat shock factor-1[J]. J Biol Chem, 1996,271(48):30847-30857
doi: 10.1074/jbc.271.48.30847
[1] Xue-mei Qing, Peng-tao Li, Jing-hong Hu, Wei-hong Li, Jin-cai Hou, Huan Du, Bing Wang, Lin Suo. Conditioned mediums of different rat cerebral microvascular endothelial cells against damage of ischemia and ischemia/reperfusion neurons. Journal of Chinese Integrative Medicine, 2007, 5(2): 183-188.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9] Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278