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Journal of Chinese Integrative Medicine ›› 2003, Vol. 1 ›› Issue (3): 218-220.doi: 10.3736/jcim20030323

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ELISA kit for detection of biliary vesicular protein and its preliminary clinical application

Duan Cai(), Jian-bin Xiang, Yan-ling Zhang, Bao-jin Ma   

  1. Surgery Department,Huashan Hospital, Fudan University,Shanghai 200040,China
  • Received:2003-03-15 Online:2003-09-20 Published:2003-09-20


To establish a rapid and precise detective method of 33.5 kd vesicular protein and to screen an effective treatment of cholelithiasis.


Specific antibody of the biliary vesicular protein was obtained by immunizing rabbits and enzyme-linked immunosorbent assay (ELISA) kit was developed. The concentrations of 33.5 kd vesicular protein in serum and bile of gallstone patients and control were examined respectively. The effects of Cholagogue Dry Syrup and Eulektrol Capsule on decreasing 33.5 kd vesicular protein were also studied by ELISA kit.


One-step ELISA equation was Y=0.035 X (r=0.99).The vesicular protein concentrations in serum and bile of cholesterol gallstone group [(179.8±97.9 ) mg/L and (213.4±70.1)mg/L respectively] were significantly (P <0.05) higher than in the pigment stone group and control. Data showed that, with 2-week administration, Cholagogue Dry Syrup significantly decreased both biliary and serum 33.5 kd vesicular protein of cholesterol gallstone patients, while Eulekrol Capsule and control groups didn’t have the same results.


The concentrations of 33.5 kd protein are different in cholesterol gallstone patients and healthy groups which might be related to cholesterol nucleation process. Cholagogue Dry Syrup is of cholagogic and litholytic effect by decreasing biliary lithogenesis.

Key words: enzyme-linked immunosorbent assay, biliary vesicular protein, Cholagogue Dry Syrup

CLC Number: 

  • R446.6-3


蛋白稀释度 1:64 1:32 1:16 1:8 1:4 1:2 1:1
OD值 0.048 0.081 0.147 0.270 0.552 1.001 2.112


组别 血清(n=50) 胆 汁(n=40)
胆固醇性结石组 179.8±97.9 213.4±70.1
胆色素性结石组 82.1±62.3* 71.9±55.4*
非胆石症胆道疾病组 55.6±52.3(n=20)* 70.9±54.7*
正常人组 75.4±58.6* 65.0±51.7*


组别 n 第1天 第3天 第7天 第14天 第21天
对照组 20 194.9±76.0 175.4±56.6 195.6±75.9 191.6±66.1* 193.1±67.8*
利胆冲剂治疗组 20 211.1±47.4 170.9±52.3 192.9±51.3 143.0±59.9 127.4±60.7
胆通胶囊治疗组 20 209.0±47.1 172.7±53.0 196.9±55.1 206.4±46.4* 209.7±51.9*


组别 n 第1天 第3天 第7天 第14天 第21天
对照组 20 43.5±14.7 29.2±12.6 42.8±14.8 41.0±14.7 43.0±13.6*
利胆冲剂治疗组 20 47.3±14.9 30.9±14.1 37.7±12.2 30.8±14.6 29.6±14.1
胆通胶囊治疗组 20 43.3±13.7 29.2±12.2 37.1±12.1 40.4±12.5 42.6±12.7*
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[3] 傅培彬, 张圣道, 郑魁元 . 168例胆石化学成份分析[J]. 中华消化杂志, 1983,3(3):170-172
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