A novel treatment for weight reduction by the recombinant “Pichia pastoris” yeast expressing the hybrid protein of “irisin-furin-transferrin”

doi:10.1016/S2095-4964(16)60242-X

Abstract

Obesity is a major health problem across the world, but there are few ways to effectively treat or manage it in the long term. Researchers are searching for more convenient, cost-effective and noninvasive therapies for overweight and obese people. Recent studies have illustrated that the microbiome of the body's different organs can be used as a vehicle for in-situ gene therapy. We suggest that the recombinant form of “Pichia pastoris” yeast expressing the hybrid protein of “irisin-furin-transferrin” under the control of the enolase 1 promoter is a new nutraceutical strategy to absorb fewer calories from intestinal nutrients, and induce a higher metabolic rate to expend more calories, similar to that from engaging in physical activity. By comparison, this method can be a long-term, noninvasive treatment and can be used for obese patients who have movement limitations.

Key words: Obesity, Nutrition, Biotechnology, DNA, Recombinant

Mahsa Jalili, Zahra Bazi, Azita Hekmatdoost. A novel treatment for weight reduction by the recombinant “Pichia pastoris” yeast expressing the hybrid protein of “irisin-furin-transferrin”. Journal of Integrative Medicine, 2016, 14(1): 1-4.

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References

1 Cao Y.Adipose tissue angiogenesis as a therapeutic target for obesity and metabolic diseases.Nat Rev Drug Discov. 2010; 9(2): 107-115.
2 Cheung WW, Mao P.Recent advances in obesity: genetics and beyond.ISRN Endocrinol. 2012; 2012: 536905.
3 Ali AT, Crowther NJ.Factors predisposing to obesity: a review of the literature.J Endocrinol Metab Diabetes South Afr. 2009; 14(2): 81-84.
4 Kandasamy AD, Sung MM, Boisvenue JJ, Barr AJ, Dyck JR.Adiponectin gene therapy ameliorates high-fat, high-sucrose diet-induced metabolic perturbations in mice.Nutr Diabetes. 2012; 2(9): e45.
5 Kopelman P.Health risks associated with overweight and obesity.Obes Rev. 2007; 8(Suppl 1): 13-17.
6 Bray GA, Tartaglia LA.Medicinal strategies in the treatment of obesity.Nature. 2000; 404(6778): 672-677.
7 Irving BA, Still CD, Argyropoulos G.Does IRISIN have a BRITE future as a therapeutic agent in humans?Curr Obes Rep. 2014; 3: 235-241.
8 Wang F, Wu M, Liu W, Shen Q, Sun H, Chen S.Expression, purification, and lipolytic activity of recombinant human serum albumin fusion proteins with one domain of human growth hormone in Pichia pastoris. Biotechnol Appl Biochem. 2013; 60(4): 405-411.
9 Wanarska M, Kur J.A method for the production of D-tagatose using a recombinantPichia pastoris strain secreting β-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c. Microb Cell Fact. 2012; 11: 113.
10 Contreras C, Gonzalez F, Fernø J, Diéguez C, Rahmouni K, Nogueiras R, López M.The brain and brown fat.Ann Med. 2015; 47(2): 150-168.
11 du Plessis L, Rose SH, van Zyl WH. Exploring improved endoglucanase expression in Saccharomyces cerevisiae strains. Appl Microbiol Biotechnol. 2010; 86(5): 1503-1511.
12 Sun X, Sun H, Ge R, Richter M, Woodworth RC, Mason AB, He QY.The low pKa value of iron-binding ligand Tyr188 and its implication in iron release and anion binding of human transferrin. FEBS Lett. 2004; 573(1-3): 181-185.
13 Moslehi-Jenabian S, Lindegaard L, Jespersen L.Beneficial effects of probiotic and food borne yeasts on human health. Nutrients. 2010; 2(4): 449-473.
14 Ortuño J, Cuesta A, Rodríguez A, Esteban MA, Meseguer J.Oral administration of yeast, Saccharomyces cerevisiae, enhances the cellular innate immune response of gilthead seabream (Sparus aurata L.). Vet Immunol Immunopathol. 2002; 85(1-2): 41-50.
15 D’Silva I. Recombinant technology and probiotics. Int [J] Eng Technol. 2011; 3(4): 288-293.
16 Castillo-Quan JI.From white to brown fat through the PGC- 1α-dependent myokine irisin: implications for diabetes and obesity. Dis Model Mech. 2012; 5(3): 293-295.
17 Mahajan RD, Patra SK.Irisin, a novel myokine responsible for exercise induced browning of white adipose tissue. Indian [J] Clin Biochem. 2013; 28(1): 102-103.
18 Bai Y, Ann DK, Shen WC.Recombinant granulocyte colony-stimulating factor-transferrin fusion protein as an oral myelopoietic agent. Proc Natl Acad Sci U S A. 2005; 102(20): 7292-7296.
19 Matysiak-Budnik T, Moura IC, Arcos-Fajardo M, Lebreton C, Ménard S, Candalh C, Ben-Khalifa K, Dugave C, Tamouza H, van Niel G, Bouhnik Y, Lamarque D, Chaussade S, Malamut G, Cellier C, Cerf-Bensussan N, Monteiro RC, Heyman M. Secretory IgA mediates retrotranscytosis of intact gliadin peptides via the transferrin receptor in celiac disease. [J] Exp Med. 2008; 205(1): 143-154.
20 Xia CQ, Wang J, Shen WC.Hypoglycemic effect of insulin-transferrin conjugate in streptozotocin-induced diabetic rats. [J] Pharmacol Exp Ther. 2000; 295(2): 594-600.
21 Cregg JM, Tolstorukov I, Kusari A, Sunga J, Madden K, Chappell T.Expression in the yeast Pichia pastoris. Methods Enzymol. 2009; 463: 169-189.
22 Griffiths WJ, Cox TM.Co-localization of the mammalian hemochromatosis gene product (HFE) and a newly identified transferrin receptor (TfR2) in intestinal tissue and cells. J Histochem Cytochem. 2003; 51(5): 613-624.
23 Wu J, Boström P, Sparks LM, Ye L, Choi JH, Giang AH, Khandekar M, Virtanen KA, Nuutila P, Schaart G, Huang K, Tu H, van Marken Lichtenbelt WD, Hoeks J, Enerbäck S, Schrauwen P, Spiegelman BM. Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human. Cell. 2012; 150(2): 366-376.

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