Search JIM Advanced Search

Journal of Integrative Medicine ›› 2016, Vol. 14 ›› Issue (1): 1-4.doi: 10.1016/S2095-4964(16)60242-X

• Commentary •     Next Articles

A novel treatment for weight reduction by the recombinant “Pichia pastoris” yeast expressing the hybrid protein of “irisin-furin-transferrin”

MahsaJalilia, ZahraBazib, AzitaHekmatdoosta   

  1. a Clinical Nutrition Department, Nutrition Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran
    b Medical Biotechnology Department, Medicine Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Received:2015-08-14 Accepted:2015-11-09 Online:2016-01-15 Published:2016-01-15
  • Contact: Zahra Bazi; Tel: +98-2123781; E-mail: zahrabazi1986@gmail.com

Obesity is a major health problem across the world, but there are few ways to effectively treat or manage it in the long term. Researchers are searching for more convenient, cost-effective and noninvasive therapies for overweight and obese people. Recent studies have illustrated that the microbiome of the body's different organs can be used as a vehicle for in-situ gene therapy. We suggest that the recombinant form of “Pichia pastoris” yeast expressing the hybrid protein of “irisin-furin-transferrin” under the control of the enolase 1 promoter is a new nutraceutical strategy to absorb fewer calories from intestinal nutrients, and induce a higher metabolic rate to expend more calories, similar to that from engaging in physical activity. By comparison, this method can be a long-term, noninvasive treatment and can be used for obese patients who have movement limitations.

Key words: Obesity, Nutrition, Biotechnology, DNA, Recombinant

1 Cao Y.Adipose tissue angiogenesis as a therapeutic target for obesity and metabolic diseases.Nat Rev Drug Discov. 2010; 9(2): 107-115.
2 Cheung WW, Mao P.Recent advances in obesity: genetics and beyond.ISRN Endocrinol. 2012; 2012: 536905.
3 Ali AT, Crowther NJ.Factors predisposing to obesity: a review of the literature.J Endocrinol Metab Diabetes South Afr. 2009; 14(2): 81-84.
4 Kandasamy AD, Sung MM, Boisvenue JJ, Barr AJ, Dyck JR.Adiponectin gene therapy ameliorates high-fat, high-sucrose diet-induced metabolic perturbations in mice.Nutr Diabetes. 2012; 2(9): e45.
5 Kopelman P.Health risks associated with overweight and obesity.Obes Rev. 2007; 8(Suppl 1): 13-17.
6 Bray GA, Tartaglia LA.Medicinal strategies in the treatment of obesity.Nature. 2000; 404(6778): 672-677.
7 Irving BA, Still CD, Argyropoulos G.Does IRISIN have a BRITE future as a therapeutic agent in humans?Curr Obes Rep. 2014; 3: 235-241.
8 Wang F, Wu M, Liu W, Shen Q, Sun H, Chen S.Expression, purification, and lipolytic activity of recombinant human serum albumin fusion proteins with one domain of human growth hormone in Pichia pastoris. Biotechnol Appl Biochem. 2013; 60(4): 405-411.
9 Wanarska M, Kur J.A method for the production of D-tagatose using a recombinantPichia pastoris strain secreting β-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c. Microb Cell Fact. 2012; 11: 113.
10 Contreras C, Gonzalez F, Fernø J, Diéguez C, Rahmouni K, Nogueiras R, López M.The brain and brown fat.Ann Med. 2015; 47(2): 150-168.
11 du Plessis L, Rose SH, van Zyl WH. Exploring improved endoglucanase expression in Saccharomyces cerevisiae strains. Appl Microbiol Biotechnol. 2010; 86(5): 1503-1511.
12 Sun X, Sun H, Ge R, Richter M, Woodworth RC, Mason AB, He QY.The low pKa value of iron-binding ligand Tyr188 and its implication in iron release and anion binding of human transferrin. FEBS Lett. 2004; 573(1-3): 181-185.
13 Moslehi-Jenabian S, Lindegaard L, Jespersen L.Beneficial effects of probiotic and food borne yeasts on human health. Nutrients. 2010; 2(4): 449-473.
14 Ortuño J, Cuesta A, Rodríguez A, Esteban MA, Meseguer J.Oral administration of yeast, Saccharomyces cerevisiae, enhances the cellular innate immune response of gilthead seabream (Sparus aurata L.). Vet Immunol Immunopathol. 2002; 85(1-2): 41-50.
15 D’Silva I. Recombinant technology and probiotics. Int [J] Eng Technol. 2011; 3(4): 288-293.
16 Castillo-Quan JI.From white to brown fat through the PGC- 1α-dependent myokine irisin: implications for diabetes and obesity. Dis Model Mech. 2012; 5(3): 293-295.
17 Mahajan RD, Patra SK.Irisin, a novel myokine responsible for exercise induced browning of white adipose tissue. Indian [J] Clin Biochem. 2013; 28(1): 102-103.
18 Bai Y, Ann DK, Shen WC.Recombinant granulocyte colony-stimulating factor-transferrin fusion protein as an oral myelopoietic agent. Proc Natl Acad Sci U S A. 2005; 102(20): 7292-7296.
19 Matysiak-Budnik T, Moura IC, Arcos-Fajardo M, Lebreton C, Ménard S, Candalh C, Ben-Khalifa K, Dugave C, Tamouza H, van Niel G, Bouhnik Y, Lamarque D, Chaussade S, Malamut G, Cellier C, Cerf-Bensussan N, Monteiro RC, Heyman M. Secretory IgA mediates retrotranscytosis of intact gliadin peptides via the transferrin receptor in celiac disease. [J] Exp Med. 2008; 205(1): 143-154.
20 Xia CQ, Wang J, Shen WC.Hypoglycemic effect of insulin-transferrin conjugate in streptozotocin-induced diabetic rats. [J] Pharmacol Exp Ther. 2000; 295(2): 594-600.
21 Cregg JM, Tolstorukov I, Kusari A, Sunga J, Madden K, Chappell T.Expression in the yeast Pichia pastoris. Methods Enzymol. 2009; 463: 169-189.
22 Griffiths WJ, Cox TM.Co-localization of the mammalian hemochromatosis gene product (HFE) and a newly identified transferrin receptor (TfR2) in intestinal tissue and cells. J Histochem Cytochem. 2003; 51(5): 613-624.
23 Wu J, Boström P, Sparks LM, Ye L, Choi JH, Giang AH, Khandekar M, Virtanen KA, Nuutila P, Schaart G, Huang K, Tu H, van Marken Lichtenbelt WD, Hoeks J, Enerbäck S, Schrauwen P, Spiegelman BM. Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human. Cell. 2012; 150(2): 366-376.
[1] Jesmin Mondal, Jayeeta Das, Rajesh Shah, Anisur Rahman Khuda-Bukhsh. A homeopathic nosode, Hepatitis C 30 demonstrates anticancer effect against liver cancer cells in vitro by modulating telomerase and topoisomerase II activities as also by promoting apoptosis via intrinsic mitochondrial pathway. Journal of Integrative Medicine, 2016, 14(3): 209-218.
[2] Jesmin Mondal, Asmita Samadder, Anisur Rahman Khuda-Bukhsh. Psorinum 6× triggers apoptosis signals in human lung cancer cells. Journal of Integrative Medicine, 2016, 14(2): 143-153.
[3] Chen Ling, Yuan Wang, Ying-lu Feng, Ya-ni Zhang, Jun Li, Xin-rui Hu, Li-na Wang, Mao-feng Zhong, Xiao-feng Zhai, Irene Zolotukhin, Arun SrivastavaChang-quan Ling. Prevalence of neutralizing antibodies against liver-tropic adeno-associated virus serotype vectors in 100 healthy Chinese and its potential relation to body constitutions. Journal of Integrative Medicine, 2015, 13(5): 341-346.
[4] Yuan-hui Zhang, Yuan Wang, Yusufali Ali Hussein, Frederick Ashby, Daniel Zhang, Zi-fei Yin, George V. Aslanidi, Arun Srivastava, Chang-quan Ling, Chen Ling. Cytotoxic genes from traditional Chinese medicine inhibit tumor growth both in vitro and in vivo. Journal of Integrative Medicine, 2014, 12(6): 483-494.
[5] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[6] Rajadurai Akilen​, Zeller Pimlott​, Amalia Tsiami​, Nicola Robinson​. The use of complementary and alternative medicine by individuals with features of metabolic syndrome. Journal of Integrative Medicine, 2014, 12(3): 171-174.
[7] Samrat Ghosh, Kausik Bishayee, Anisur Rahman Khuda-Bukhsh​. Oleanolic acid isolated from ethanolic extract of Phytolacca decandra induces apoptosis in A375 skin melanoma cells: Drug-DNA interaction and signaling cascade. Journal of Integrative Medicine, 2014, 12(2): 102-114.
[8] Daniel A. Weber, Janelle M. Wheat , Geoffrey M. Currie. Cancer stem cells and the impact of Chinese herbs, isolates and other complementary medical botanicals: A review. Journal of Chinese Integrative Medicine, 2012, 10(5): 493-503.
[9] Das Sreemanti, Kumar Saha Santu, De Arnab, Das Durba, Rahman Khuda-Bukhsh Anisur. Potential of the homeopathic remedy, Arnica Montana 30C, to reduce DNA damage in Escherichia coli exposed to ultraviolet irradiation through up-regulation of nucleotide excision repair genes. Journal of Chinese Integrative Medicine, 2012, 10(3): 337-246.
[10] De Arnab, Das Durba, Dutta Suman, Chakraborty Debrup, Boujedaini Naoual, Rahman Khuda-Bukhsh Anisur. Potentized homeopathic drug Arsenicum Album 30C inhibits intracellular reactive oxygen species generation and up-regulates expression of arsenic resistance gene in arsenite-exposed bacteria Escherichia coli. Journal of Chinese Integrative Medicine, 2012, 10(2): 210-227.
[11] Min Zhu , Mi Zhou , Ying Shi , Wen-wei Li. Effects of echinacoside on MPP+-induced mitochondrial fragmentation, mitophagy and cell apoptosis in SH-SY5Y cells. Journal of Chinese Integrative Medicine, 2012, 10(12): 1427-1432.
[12] Stephen D. Anton, Jonathan Shuster, Christiaan Leeuwenburgh. Investigations of botanicals on food intake, satiety, weight loss and oxidative stress: Study protocol of a double-blind, placebo-controlled, crossover study. Journal of Chinese Integrative Medicine, 2011, 9(11): 1190-1198.
[13] Xian-juan Kong , Lei Gao, Hao Peng, Xian Shi. Effects of electro-acupuncture on expression of obestatin in hypothalamus of rats with simple obesity. Journal of Chinese Integrative Medicine, 2010, 8(5): 480-485.
[14] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035.
[15] Jun-shan Huang, Wei-bo Zhang, Xing-min Zheng, Qiu-cheng Lin , Jing-yi Li, Zuo-dan Zhang, Jian Lin. Effects of Ruanmailing Oral Liquid on spatial learning and memory ability and expression of APE/Ref-1 in hippocampal CA1 region in rats with experimental vascular dementia. Journal of Chinese Integrative Medicine, 2009, 7(9): 855-.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[2] Peng-fei Gao, Kenji Watanabe. Introduction of the World Health Organization project of the International Classification of Traditional Medicine. Journal of Chinese Integrative Medicine, 2011, 9(11): 1161-1164
[3] Mei-jun Lü, Hui-yong Zhang, Zhe Zhang, Guan-lin Yang. Measurement of health-related quality of life in coronary heart disease: A review. Journal of Chinese Integrative Medicine, 2011, 9(12): 1277-1285
[4] Jia-bin Tai, Xu-bo Shen, Lin Li, Wen-qi Liu, De-zeng Zhu, Chang-quan Ling. Discussion of some problems in studying relationship between sphygmo-diagram and syndrome types or diseases. Journal of Chinese Integrative Medicine, 2005, 3(1): 10-13
[5] Hui-hua Zhu, Yan-ping Chen, Jian-er Yu, Min Wu, Zhan Li. Therapeutic effect of Xincang Decoction on chronic airway inflammation in children with bronchial asthma in remission stage. Journal of Chinese Integrative Medicine, 2005, 3(1): 23-27
[6] Kwan Leung Chia. Symptomatic Tarlov cyst and electroacupuncture: More studies required. Journal of Integrative Medicine, 2015, 13(1): 58-60
[7] Majid Nimrouzi, Mohammad M. Zarshenas. Functional constipation in children: Non-pharmacological approach. Journal of Integrative Medicine, 2015, 13(2): 69-71
[8] Pei-ting Zhu, Jing-zhe Zhang, Xue-lin Zhang, Zuo-biao Yuan, Hong-ying Zhang, Ju Gao. Effects of Shengqing Capsules on cholelithiasis-related genes in guinea pigs. Journal of Chinese Integrative Medicine, 2005, 3(3): 207-210
[9] Hui Li, Chun Li, Shao-hui Du, Yi-wei Li, Dong-feng Chen. Influence of electro-acupuncture of Neiguan on plasmic concentrations of NO and TNFα in endotoxin shock rats. Journal of Chinese Integrative Medicine, 2003, 1(4): 281-284
[10] Li-jun Xu, Yong-hong Hu, Fu-er Lu, Xin Zou. Experimental study on anti-inflammatory and analgesic effects of Yitieling Paste. Journal of Chinese Integrative Medicine, 2005, 3(4): 303-306