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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (1): 54-56.doi: 10.3736/jcim20050116

• Technique and Methods • Previous Articles     Next Articles

Determination of baicalin in Xiaoyanling Decoction by high-performance liquid chromatography

Jun-yi Jiang,Xiang Li,Rong-li Qiu,Hai-xia Zhang,Zhi-ping Wu   

  1. School of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China
  • Received:2004-04-21 Online:2005-01-20 Published:2005-01-20
  • Contact: Xiang Li E-mail:lxiang-mail@sohu.com

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Objective

To establish a high-performance liquid chromatography (HPLC) method for the determination of baicalin in Xiaoyanling Decoction.

Methods

HPLC method was used for determination. A Kromosil C18 column was used. The mobile phase consisted of MeOH-acid water (H2O∶H3PO4=53∶0.2) 40∶60, and the detective wavelength was 277 nm.

Results

The content of baicalin in each Xiaoyanling Decoction sample was not less than 0.40 mg/ml (RSD<5%).

Conclusion

The content of baicalin in Xiao-yanling Decoction is high and stable, and it can be used as the quality standard for Xiaoyanling Decoction.

Key words: Xiaoyanling Decoction, Baicalin, High-performance liquid chromatography

CLC Number: 

  • R284.1

Fig 1

Chromatogram of 3 samples Negative sample; B: Standard control; C: Tested sample"

Tab 1

Test result of recovery rate"

Sample Added （mg） Tested （mg） Recovery rate （%） Average （%） RSD （%）
1 0.389 6 0.880 15 102.1 9.12 2.7
2 0.389 6 0.878 86 101.7
3 0.480 7 0.971 80 100.4
4 0.480 7 0.952 20 96.4
5 0.584 4 1.056 52 98.2
6 0.584 4 1.042 88 95.9

Tab 6

Test result of content of baicalin in Xiaoyanling Decoction"

Lot Sample Content of baicalin （mg/ml） Average （mg/ml） RSD （%）
1 0.482 49
021106 2 0.469 32 0.475 89 1.30
3 0.475 86
4 0.467 17
021107 5 0.466 50 0.469 68 1.05
6 0.475 37
7 0.495 83
021108 8 0.454 10 0.454 77 2.36
9 0.444 38
10 0.411 11
021109 11 0.400 91 0.406 20 1.26
12 0.406 59
13 0.489 80
021110 14 0.500 18 0.497 97 1.47
15 0.503 94
16 0.459 46
021111 17 0.462 27 0.459 54 0.59
18 0.456 88
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