高效液相色谱法同时测定健康人口服加味逍遥散后血清阿魏酸和芍药苷含量及其药动学研究

doi:10.3736/jcim20081114

Abstract

Objective:

To simultaneously determine the contents and explore the pharmacokinetics of ferulic acid and paeoniflorin by high performance liquid chromatography (HPLC) after oral administration of Modified Xiao-yao Decoction (MXYD), a compound of traditional Chinese herbal medicine.

Methods:

A total of 8 healthy men were enlisted in this study. The serum samples were preprocessed by immersion method. The HPLC system was used to determine the contents of ferulic acid and paeoniflorin in the blood samples of the 8 healthy volunteers, and the blood was collected through the ulnar vein at 5 min, 10 min, 15 min, 30 min, 45 min, 1 h, 2 h and 3 h after MXYD administration. The dates of serum concentration-time were fitted by using the 3P97 analytical program of pharmacokinetics. The internal standard (IS) was coumarin. The detection wavelengths of paeoniflorin and ferulic acid were 230 nm from 0 min to 10 min and 320 nm from 10 min to 25 min respectively.

Results:

After MXYD administration, paeoniflorin and ferulic acid were separated completely in the serum and no other interfering peaks were found in the spectrum of the chromatograms. The retention times of the paeoniflorin and ferulic acid were 8.02 min and 13.32 min respectively, and that of the coumarin was 19.14 min. The mean recovery rates of paeoniflorin and ferulic acid were 100.9% and 95.3% with relative standard deviation (RSD) of 3.2% and 3.4%, respectively. The calibration curve for paeoniflorin was linear over the concentration range 40-1 280 ng/ml. Its low-detection limit based on a signal-to-noise ratio of 3 was 5 ng/ml and low-concentration limit was 15 ng/ml with an RSD of 12.5%. Ferulic acid was in the range 10-320 ng/ml; its low-detection limit was 0.65 ng/ml and low-concentration limit was 5 ng/ml with an RSD of 9.7%. The method was found to be highly precise, with an RSD<5% and interday and intraday variability in the range of 92.1%-109.9% for each of the concentrations tested.

Conclusion:

This is a study on simultaneously determining paeoniflorin and ferulic acid in serum of healthy volunteers after oral administration of MXYD. The assay is suitable for identifying the serum levels of ferulic acid and paeoniflorin in clinical investigations.

Key words: Jiawei Xiaoyao Powders, paeoniflorin, ferulic acid, high performance liquid chromatography, pharmacokinetics

CLC Number:

R969.1

Hui Li, Ping Ren, Xi Huang, Wen-fu Tang, Hong-qiang Wei. Simultaneous determination and pharmacokinetic studies of ferulic acid and paeoniflorin in human serum by high performance liquid chromatography after oral administration of Modified Xiao-yao Decoction. Journal of Chinese Integrative Medicine, 2008, 6(11): 1178-1183.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20081114

http://www.jcimjournal.com/EN/Y2008/V6/I11/1178

TrendMD

Figures/Tables 7

Figure 1

Figure 2

Table 1

Table 2

Table 3

Figure 3

Table 4

References 6

[1]	Yu GH, Liang SC, Sun QZ , et al. Study on modified Xiaoyao Decoction combining clomipamine in treating depression[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2007,27(4):318-320
	余国汉, 梁绍财, 孙祺章 , 等. 加味逍遥汤合并氯米帕明治疗抑郁症的对照研究[J]. 中国中西医结合杂志, 2007,27(4):318-320
[2]	Ren P, Huang X, Li SQ , et al. Effect of modified Xiaoyao powder on electrogastrographic picture and pharmacokinetic characteristics of ferulic acid in patients with functional dyspepsia of Gan-qi stagnation with Pi-deficiency syndrome type[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2006,26(5):398-402
	任平, 黄熙, 李双庆 , 等. 加味逍遥散对功能性消化不良肝郁脾虚型患者胃电图及阿魏酸药动学特征的影响[J]. 中国中西医结合杂志, 2006,26(5):398-402
[3]	Yang YX, Huang X, Ren P , et al. HPLC determination of concentration of ferulic acid in blood of patients with liver depression and spleen deficiency[J]. Nanjing Zhong Yi Yao Da Xue Xue Bao, 2003,19(2):86-88
	杨玉兴, 黄熙, 任平 , 等. 肝郁脾虚证患者口服加味逍遥散后阿魏酸的血药浓度HPLC测定法[J]. 南京中医药大学学报, 2003,19(2):86-88
[4]	Yang XG, Peng B, Zhang GH , et al.Studies of the pharmacokinetics of paeoniflorin in two Jing-Zhi-Guan-Xin formulations after oral administration to beagle dogs[J].J Pharm Biomed Anal, 2006, 41: 320-324 doi: 10.1016/j.jpba.2005.11.004
[5]	Luo HM, Li XG, Xiao F , et al. Study on pharmacokinetics of ferulic acid and paeoniflorin in Angelic-Paeonia Root Powder[J]. Zhong Yao Cai, 2003,26(3):189-192 doi: 10.3321/j.issn:1001-4454.2003.03.017
	罗焕敏, 李晓光, 肖飞 . 当归芍药散中阿魏酸和芍药苷的药代动力学研究[J]. 中药材, 2003,26(3):189-192 doi: 10.3321/j.issn:1001-4454.2003.03.017
[6]	Xu S, Hu JH . Determination of sodium ferulate in Beagle dog serum by HPLC and its pharmacokinetics[J]. Zhong Cheng Yao, 2005,9(27):1062-1065
	徐术, 胡晋红 . HPLC测定Beagle犬血浆中阿魏酸钠的浓度及药动学研究[J]. 中成药, 2005,9(27):1062-1065

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2]	Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3]	Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4]	Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5]	. Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6]	Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7]	Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8]	Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9]	Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10]	Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228

Component	n	Nominal concentration (ng/ml)	Recovery rate (x±s, %)	Mean of recovery rate (%)	RSD (%)	Mean of RSD (%)
Ferulic acid	8	10.0	88.6±1.9	95.3	4.2	3.2
	8	40.0	98.7±2.4	95.3	2.9	3.2
	8	160.0	98.6±2.0	95.3	2.5	3.2
Paeoniflorin	8	40.0	103.4±2.3	100.9	4.9	3.4
	8	160.0	99.3±4.1	100.9	3.8	3.4
	8	640.0	100.2±3.4	100.9	1.6	3.4

Component	n	Nominal concentration (ng/ml)	Measured concentration (x±s, ng/ml)	Interday variability (%)	RSD (%)
Ferulic acid	5	10.0	9.37±0.46	93.7	4.9
	5	40.0	40.70±1.81	101.7	4.4
	5	160.0	172.77±6.54	100.9	3.8
Paeoniflorin	5	40.0	43.15±1.53	108.0	3.5
	5	160.0	160.22±4.89	100.1	3.1
	5	640.0	589.24±24.92	92.1	4.2

Component	n	Added concentration (ng/ml)	Measured concentration (x±s, ng/ml)	Intraday variability (%)	RSD (%)
Ferulic acid	3	10.0	9.63±0.40	96.3	4.2
	3	40.0	41.65±1.56	104.1	3.7
	3	160.0	170.42±8.06	106.5	4.7
Paeoniflorin	3	40.0	43.99±1.92	109.9	4.4
	3	160.0	158.83±5.24	99.3	3.3
	3	640.0	621.81±25.73	97.2	4.1

Component	n	Tmax (x±s, min)	Cmax (x±s,μg/L)	t1/2α (x±s, min)	CL/F [x±s, L/(min·kg)]	AUC(0-3 min) [x±s, μg/(L·h)]	MRT(0-3 min) (x±s, min)
Ferulic acid	8	45.00±11.34	70.81±7.17	33.39±20.80	714.399±187.451	6 434.77±1 192.56	66.910±10.461
Paeoniflorin	8	43.13±9.61	536.08±49.03	55.70±25.26	66.310±24.606	71 639.31±16 625.00	121.200±17.242

Simultaneous determination and pharmacokinetic studies of ferulic acid and paeoniflorin in human serum by high performance liquid chromatography after oral administration of Modified Xiao-yao Decoction

RichHTML

PDF (PC)

Like

Abstract

Cite this article

Share this article